Acridine ester chemiluminescence reagent is a commonly used fluorescent marker, and its fluorescence signal can accurately provide information. Its luminescent properties make it an important tool in scientific research, with extensive applications in various fields such as biology, medicine, and chemistry, especially in drug development and biomedical research, providing strong support for scientific experiments. Below, we will take you to a deeper understanding of acridine esters.
The Luminescence Principle of Acridine Esters
According to the different substituents, acridine substituents commonly used as chemiluminescent markers can be divided into two categories: acridine esters and acridine sulfonamides. They all have the same acridine ring in their structures. Their luminescence mechanism is the same: the molecules of acridine esters are attacked by hydrogen peroxide ions in alkaline H2O2 solution, so the substituents on the acridine ring can form unstable ethylene oxide with C-9 and H2O2 on the acridine ring, which can be decomposed into CO2 and N-methylacridone. When it returns to the ground state, it emits photons with a maximum emission wavelength of 430nm. This process does not require a catalyst, the luminescent system is sensitive, and the operation is time-saving.
Characteristics of Acridine Esters
1. Acridine ester emits simple light without the need for additional additives
Acridine ester has simple luminescence and does not require any additional catalysts. It can reduce background luminescence, reduce interference, and improve sensitivity during experiments.
2. Acridine ester with high luminescence efficiency and intensity
The chemiluminescence of acridine esters is rapid and typically performs exceptionally well in the field of chemiluminescence analysis. After adding the initiator for 0.4 seconds, the chemiluminescence intensity of acridine ester is very high, with a half-life of 0.9 seconds, which is usually 5 times or even more than that of luminol.
3. The interference factor of acridine ester luminescence is small
From the above luminescence principle, it can be seen that during the luminescence reaction of acridine ester, when an electron excited intermediate is formed in the early stage of the reaction, the non luminescent substituent connected to the acridine ring is separated from the acridine ring, so the luminescence efficiency is almost not affected by the interference of the substituent structure.
The practical application of acridine ester
1. Fluorescent dyes: Acridine ester, as an excellent fluorescent dye, is widely used in biomedical fields such as cell imaging and protein detection. Its luminescent properties can help experimenters observe and monitor the behavior of biomolecules in real time.
2. Fluorescent labeling: In chemical and biological analysis, acridine ester can be used as a fluorescent marker for tracking and detecting specific molecules or compounds, which is of great significance in drug development and scientific research.
Precautions for using acridine ester
1. To avoid the environmental impact on the luminescence sensitivity of acridine esters, attention should be paid to factors such as solvent selection, pH value, and temperature during the experiment.
2. Acridine ester may have certain irritancy, and safety precautions should be taken during use. At the same time, due to the yellow powder of acridine ester, its stability is easily affected by the environment. Therefore, it is necessary to choose non transparent plastic bottles for sealed storage to reduce potential risks.
2. Acridine esters may cause damage and pollution to the environment in some experiments, and their release needs to be strictly controlled during use. Safety and protection measures need to be formulated in production and use, and the waste after use should be properly disposed of according to relevant regulations.
Acridine ester, as a luminescent organic compound, provides a powerful tool for scientific research and application in many fields. Its luminescent environment is affected by various factors, so operating guidelines should be followed and timely observation should be made when using it. As a manufacturer of acridine ester, Desheng can provide raw materials with a purity of up to 98%. Not only does it emit light sensitively and stably, but it is also sold at the manufacturer's spot price with great discounts! If you have any relevant intentions, please feel free to contact us for purchase at any time!
