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CAS 29915-38-6 N-Tris (Hydroxymethyl) Methyl-3-Aminopropanesulfonic Acid

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CAS 29915-38-6 N-Tris (Hydroxymethyl) Methyl-3-Aminopropanesulfonic Acid

CAS 29915-38-6 N-Tris (Hydroxymethyl) Methyl-3-Aminopropanesulfonic Acid
CAS 29915-38-6 N-Tris (Hydroxymethyl) Methyl-3-Aminopropanesulfonic Acid CAS 29915-38-6 N-Tris (Hydroxymethyl) Methyl-3-Aminopropanesulfonic Acid CAS 29915-38-6 N-Tris (Hydroxymethyl) Methyl-3-Aminopropanesulfonic Acid

Large Image :  CAS 29915-38-6 N-Tris (Hydroxymethyl) Methyl-3-Aminopropanesulfonic Acid

Product Details:
Place of Origin: hubei
Brand Name: desheng
Certification: ISO9000
Model Number: AR
Payment & Shipping Terms:
Minimum Order Quantity: 500g
Price: Consult the latest price
Packaging Details: 500g / bottle, 25kg / barrel
Delivery Time: 5 days
Payment Terms: L/C, D/P, T/T, Western Union
Supply Ability: 1000kg/a week
Detailed Product Description
Brand: Desheng Purity: >99%
Molecular Formula: C7H17NO6S Molecular Weight: 243.28
EINECS: 249-954-1 Mol: 29915-38-6.mol
High Light:

CAS 29915-38-6 3 aminopropane sulfonic acid

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29915-38-6 3 aminopropane sulfonic acid

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99% 3 aminopropane sulfonic acid

Application of n-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid biological buffer,CAS29915-38-6 supplier

 

Taps, n-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid, is a member of the good's buffer family. It is a kind of amphoteric buffer widely used in biochemistry and molecular biology. It is often used as a buffer for the separation of dyes in planar chromatography and for the purification of enzymes in ion exchange chromatography.

 

appearance White powder
nature Soluble in water
PH buffer range 7.7-9.1
pKa(25℃) 8.40

 

1, Biological buffer for the separation of dyes in chromatography

 

CAS 29915-38-6 N-Tris (Hydroxymethyl) Methyl-3-Aminopropanesulfonic Acid 0

Picture: taps is used for chromatographic separation of dyes

 

Chromatography is a laboratory technique, usually used to separate and purify proteins. In this process, the separation ability of the system is directly related to the change of pH. For example, if the pH value of the mobile phase (solvent) is close to pKa, a small change in the pH value will seriously affect the retention rate, thus leading to separation. Since the retention of ionizable compounds is particularly sensitive to the pH of the mobile phase, a buffer should be added to the system to control this variable.

 

Based on the most relevant literature on chromatography, Desheng's researchers found that although Tris and MES are generally considered to be the best choice, other buffers including taps have also been used in cation exchange chromatography, anion exchange chromatography, high performance liquid chromatography (HPLC) and other similar technologies.

 

2, One of the most suitable biological buffers for cell culture

 

CAS 29915-38-6 N-Tris (Hydroxymethyl) Methyl-3-Aminopropanesulfonic Acid 1

Picture: taps for cell culture

 

As mentioned above, taps is a member of the good's family of buffers. One of the most important features of good's buffers is that they are non-toxic to cells. Therefore, these chemicals are widely used in cell culture to keep the pH value under control. Due to their special functions, many good's Buffers / biological buffers are considered ideal for cell isolation, cell culture, enzyme analysis and many other biochemical applications.


Desheng's researchers found that protonated sulfamate compounds directly inhibit the activity of connexin channels. In good's pH buffer (MES (4-morpholine ethane sulfonic acid), HEPES and taps (3 - {[2-hydroxy-1,1-bis (hydroxymethyl) ethyl] amino] - 1), pH dependent connexin channel activity demonstrated this. -Propane sulfonic acid), which share a common sulfamate moiety and have no pH dependent channel activity in pH buffer without sulfamate moiety. Therefore, taps is more used for flagellate algae experiment in cell culture medium.

 

CAS 29915-38-6 N-Tris (Hydroxymethyl) Methyl-3-Aminopropanesulfonic Acid 2

 

Hubei xindesheng Material Technology Co., Ltd. is committed to the R & D, production and sales of biological buffers. Can provide pure white appearance products, products with high purity, small batch difference and other characteristics. Welcome to contact us www.whdsbio.com.

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