Payment & Shipping Terms:
|Molecular Weight:||121.135||Molecular Formula:||C4H11NO3|
|Appearance:||White Crystal Powder||Storage Conditions:||Room Temperature, Light-proof And Moisture-proof|
PH 7.1 Tris(Hydroxymethyl)Amino Methane,
Hcl Tris(Hydroxymethyl)Amino Methane,
PH 7.1 hydroxymethyl aminomethane
Precautions for purchasing tris (CAS77-86-1) when used in different industries
Tris(hydroxymethyl)aminomethane, commonly referred to as Tris, also called tromethamine, is a very commonly used reagent and is widely used in the fields of industrial synthesis, biochemical testing, and biopharmaceuticals; it is used in virus preservation liquid sampling tubes It is an important raw material in the configuration solution. The issues that should be paid attention to in the procurement of these three application fields are different. So what are the precautions? Desheng summarizes 4 points here. I hope to be able to You guys are helpful.
|PH value required(25℃)||7.10||7.20||7.30||7.40||7.50||7.60||7.70||7.80||7.90||8.00||8.10||8.20||8.30||8.40||8.50||8.60||8.70||8.80||8.90|
|0.1mol/L HCI volume||45.7||44.7||43.4||42||40.3||38.5||36.6||34.5||32||29.2||26.2||22.9||19.9||17.2||14.7||12.4||10.3||8.5||7
Desheng Tris TRIS Packaging
1. Tris is used in the direction of industrial synthesis
Because of its simple structure, tris hydroxymethyl amino methane has a tetrahedral structure with a central carbon atom, containing three methylol groups and one amino group, it can undergo a variety of substitutions and redox reactions. It is a high-quality chemical synthesis raw material. A synthetic method of buffer TAPS is to synthesize Tris and 1,3-propane sultone in alcohol. Usually, the Tris raw materials to be purchased for industrial synthesis do not require high reagent purity. The focus is on large quantities. It is more cost-effective to find raw materials manufacturers with larger production capacity.
2. Tris is used for biochemical detection direction
Tris is usually used as a biological buffer to maintain the pH of the biochemical reaction. Because it is weakly alkaline, it is usually used with acid. Mainly used for nucleic acid, protein dissolution buffer, Western blot electrophoresis buffer, Southern blot experiment buffer, DNA agarose gel electrophoresis buffer, etc. Because of the participation of enzymes, proteins and various antibodies in the reaction system, the requirements for buffers are relatively high. Therefore, when purchasing, you need to choose a manufacturer with many years of R&D and production experience. High purity and low impurities can ensure the accuracy of biochemical experiments. Sex. The TRIS buffer has a large temperature effect and is easy to absorb carbon dioxide in the air. The buffer must be tightly sealed.
Desheng Tris Stocking Status
3. Tris is used in medical research and development
There is another alias for trishydroxymethylaminomethane, tromethamine, which is a sodium-free amino buffer base that reacts with H2CO3 in body fluids to reduce H2CO3 and at the same time generate HCO32-, which can take up hydrogen ions. The correction of acidemia has a strong effect and can penetrate cell membranes. It is often used for acute metabolic and respiratory acidemia; as an alkaline drug, it is used to correct acidosis without increasing carbon dioxide retention. Because it is used in pharmaceuticals, Tris has strict requirements on the source of the entire production raw materials, production process routes, product purity, and impurity ion content. The procurement of this type of product must be very strict and cautious, and must undergo strict testing.
4. Tris is used in virus preservation solution
In the virus preservation solution, adding Tris can first maintain the pH value of the sampled sample and act as a biological buffer.
In addition, the pH buffer range of Tris: 7.0-9.0, can maintain the stability of the nucleic acid released after the lysis of the sample to be extracted, to avoid degradation of the nucleic acid, improve the concentration and purity of the nucleic acid, and ensure the accuracy of subsequent nucleic acid detection and analysis operations. Because of the special period of the epidemic, the demand for nucleic acid testing will increase, and the purchase volume of Tris will also increase. Therefore, it is recommended to purchase a small amount of products before trying to confirm that there is no problem, and then buy in large quantities. In addition, there must be technical after-sales professional services. Technical Support.
In addition to considering the application direction, the procurement of Tris sometimes needs to consider its preparation process, but the current domestic situation except
In addition to the higher requirements for pharmaceuticals, other fields mainly require purity and impurities. Tris(hydroxymethyl)aminomethane produced by Desheng
It is mainly used in the field of biochemical reagents and chemical synthesis. The quality and price are very ideal. Welcome to discuss cooperation.
Contact Person: Sales Manager