There are many kinds of additives for blood collection, such as anticoagulants, accelerators, separation gels, etc. In order to distinguish the use of different blood collecting vessels and additives, different cap colors are used internationally to mark blood collecting vessels. The functions of different color caps are different, some added anticoagulants, some added accelerators, and some blood collecting vessels without any additives. What is the role of different additives in blood collection? Desheng, as an old manufacturer of vascular additive, can help you solve your doubts.
Red head: no additives, common serum tube for routine biochemical and immunological tests.
Orange red head cap: coagulant, can activate fibrin, make soluble fibrin into insoluble fibrin polymer, and then form a stable fibrin clot. The rapid serum tube can coagulate the collected blood in 5 minutes, which is suitable for emergency series tests.
Golden head cover: separation gel and accelerator. After centrifugation, the separation gel can completely separate the liquid components (serum or plasma) and solid components (red blood cells, white blood cells, platelets, fibrin, etc.) in the blood, and completely accumulate in the center of the test tube to form a barrier. The sample remains stable within 48 hours. Coagulant can quickly activate the coagulation mechanism and accelerate the coagulation process, which is suitable for emergency series tests.
Green head cover: heparin, suitable for hemorheology, erythrocyte fragility test, blood gas analysis, hematocrit test, general biochemical determination. Heparin has the function of antithrombin and can prolong the clotting time of samples, so it is not suitable for hemagglutination test. Excessive heparin can cause leukocyte aggregation and cannot be used for leukocyte count. Because it can make the background of blood stained light blue, it is not suitable for morphological examination.
Light green head cover: separation gel and heparin lithium can achieve the purpose of rapid separation of plasma. It is the best choice for electrolyte detection. It can also be used for routine plasma biochemical detection and emergency plasma biochemical detection in ICU.
Purple head cover: EDTA anticoagulant, the anticoagulant is ethylenediamine tetraacetic acid (EDTA), which can combine with calcium ion in blood to form chelate, and make Ca2 + lose coagulation, so as to prevent blood coagulation. It is suitable for many kinds of hematological examination. However, EDTA can affect platelet aggregation and is not suitable for coagulation test, platelet function test, calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and PCR test.
Light blue head: sodium citrate anticoagulant, sodium citrate mainly through chelating with calcium ions in blood samples and anticoagulant effect, suitable for coagulation test.
Black head cover: sodium citrate ESR test tube, the concentration of sodium citrate required for ESR test is 3.2% (equivalent to 0.109 mol / L), and the ratio of anticoagulant to blood is 1 ∶ 4.
Gray head cover: potassium oxalate / sodium fluoride. Sodium fluoride is a weak anticoagulant. It is usually used in combination with potassium oxalate or sodium ethyliodate. The ratio is 1 part of sodium fluoride and 3 parts of potassium oxalate. It can not be used in the determination of urea by Urease method, nor in the determination of alkaline phosphatase and amylase. It is recommended to be used in the determination of blood glucose.
What we should pay attention to after blood collection:
A. After the injection of blood, pay attention to shake well, the force should be moderate to prevent hemolysis.
B. The amount of blood injected should be strictly in accordance with the volume marked on the test tube.
C. The blood from different color tubes should not be poured into other color tubes, otherwise it will lead to wrong results.
D. The needle should not contact the wall of the test tube to avoid contact with anticoagulant, resulting in wrong results.
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