||ADOS/N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methoxyaniline sodium salt dihydrate
||Light Yellow Powder
The chromogenic substrate ADOS is a kind of raw material used in the biochemical kit for color development, CAS No. 82692-96-4, which can react with 4-AAP in the presence of peroxidase catalyzed by hydrogen peroxide to produce a chromogenic reaction. The chromogenic reaction is sensitive and the detection is rapid.Guidelines for the use of ADOS reagents manufactured by our company are provided here for reference only.
Appearance and packaging of ADOS reagent
The packaging box is foam incubator, containing blue ice or ice bag, with product instructions and test reports. If incomplete, please call customer service for electronic version.The reagent is packaged in dark brown bottles and white crystalline powder. If the color deepens, it may have grown bacteria or partially oxidized and cannot be used.
ADOS reagent bottle
After receipt, the built-in ice pack has melted, will it cause the product to deteriorate?
The product is stable at room temperature, and low temperature transportation is to prevent extreme temperature conditions that may occur during transportation.So when you receive the product, if the ice pack has been melted, it will not affect its quality, please be assured to use it.If long-term storage is recommended to be frozen and protected from light, ready-to-use solution is recommended to be prepared to avoid oxidative discoloration in contact with air.
The chromogenic substrate ADOS is a highly water-soluble sodium aniline derivative modified from the traditional chromogenic phenol and aniline.Reagent powder may be attached to the tube wall or bottle wall, allowing low-speed centrifugation with a centrifuge to reduce product loss; the product has good solubility and can be dissolved directly with deionized water; double-distilled water or ultrapure water can be used for higher experimental requirements; when special circumstances require DMSO as a solvent, its solubility in DMSO should be consulted first, and a corresponding DMSO concentration control group should be set up;In special cases, water bath or ultrasonic vibration can be used to assist dissolution; when the concentration changes too quickly during the dilution process and the substance precipitates, ultrasonic method can be used to reconstitute it.
Whether the product is sterile
This product is powdered reagent, which is cryopreserved to reduce the possibility of long-term bacteria in solution reagents. When preparing solution, it is only necessary to keep the operating environment and equipment and instruments sterile. If sterilization is really necessary, it is recommended to use bacterial filters, and do not sterilize at high temperature and high pressure.
When ADOS is used as chromogenic substrate to participate in the hydrogen peroxide coupling chromogenic reaction, peroxidase is required. Therefore, the pH of the reaction system is strictly required. It is suggested that the biological buffer produced by Desheng Technology be used to adjust the reaction and the environmental pH to ensure the activity of the enzyme and improve the detection sensitivity.