Chemiluminescent Reagent ME-DMAE-NHS Yellow Powder or solid High purity

Product Name: 2',6'-Dimethylcarbonylphenyl 10-Methyl-9-acridinecarboxylate 4'-NHS Ester Triflate

CAS NO:115853-74-2

Acridine ester ME-DMAE-NHS-Hubei New Dehseng Materials Technology Co., Ltd. specializes in the research, development, production and sales of blood collection tube additives, in vitro diagnostic reagents, buffers and luminescent substrates. In the aspect of blood collection tube additives, it has formed independent research and development capabilities with independent intellectual property rights. Providing products and raw material solutions for more than 100 domestic and foreign manufacturers.

Acridine esters ME-DMAE-NHS and related compounds have proven to be very advantageous chemiluminescent labels with stability, activity and sensitivity over those of radioisotopes. Acridinium esters react with any amino-containing protein. Under alkaline conditions, the NHS will leave and the acridinium ester will combine with the protein to form an acridine compound with a stable amide bond. After the reaction is completed, the excess acridine salt is removed through a desalting column. In the presence of basic hydrogen peroxide, the acridine-labeled protein does not require enzymatic catalysis to self-luminesce. Immediately after the addition of the excitation reagent, the photons are released and can be detected using a 430 nm standard luminometer. This illuminating process is very short (the whole process takes less than 2 seconds) and the triggering scheme must increase the internal photometer and photon detector. Proteins, peptides, antibodies, and nucleic acids can all be labeled with acridine. The labeled compound illuminates rapidly under the excitation of alkaline hydrogen peroxide, and the labeled compound can be detected by collecting photons.
Main uses: Chemiluminescence and immunoassay, receptor analysis, nucleic acid and peptide detection.

Principle of illumination
In an alkaline H2O2 solution, the acridinium ester is attacked by hydrogen peroxide ions to form a strained, unstable dioxyethane which is further decomposed into CO2 and an electronically excited acridone, when the acridone is returned. In the ground state, it emits * absorbs photons with a wavelength of 430 nm.

                               Chemiluminescent Reagent ME-DMAE-NHS powder

Product characteristics
1 Luminescence reaction Before the formation of the electronically excited intermediate, the non-luminous substitution moiety attached to the acridine ring is separated from the luminescent moiety, and thus its luminous efficiency is substantially unaffected by the substituent structure.
2 No catalyst, no need for enhancer, it can emit light in dilute alkaline solution with H2O2.
3 The type of luminescence is flash type. After adding the excitation liquid, the luminescence intensity is about 0.4s and the half-life is about 0.9s.

product advantages
1 low background illumination, high signal to noise ratio
2 luminescence reaction, less interference factors
3 Photon release is fast concentrated, high luminous efficiency, and high luminous intensity
4 Easy to couple with protein, the photon yield will not decrease after coupling, increase sensitivity, and can be stored for several months at 2-8 °C
5 The solid phase separation agent is a very fine magnetic powder. In addition to increasing the coating area and accelerating the reaction, it can also be cleaned at the same time.

Product stability

1 It is stable in acidic solution (pH<4.8). When stored with protein conjugate for 4 weeks at room temperature, its photon yield does not decrease. The lyophilized product can be stored for more than one year at -20 °C.
2 When pH>4.8, especially in alkaline solution, the acridine compound is partially hydrolyzed to reduce its stability, and the hydrolysis process is a dark reaction process that does not emit light; the degree of hydrolysis increases with increasing pH, and rises with temperature. High and increase.
3 acridine amide stability is higher than acridine ester structure, strong resistance to hydrolysis.
4 Acridine ring or phenol ring or benzenesulfonyl ring is connected with a donor group such as a methyl group. Due to its large steric hindrance, the thermal stability increases. The electron-withdrawing group is attached to facilitate the nucleophilic substitution reaction. The stability is declining.
5 After some users feedback the protein label, the amount of luminescence decreases after a period of time. Suggestions:
The buffer used for storage after coupling should be as weak as possible, and the oxygen should be removed by nitrogen gas. Sealed and protected from light and stored at low temperature. Conditionally, it can be made into lyophilized sample and then stored.