Cas No 29915-38-6 99% Good Buffer Solutions Biological Buffer TAPS

buffer solutions；PREPARATION METHOD OF TAPS; GOOD BUFFER;CAS NO 29915-38-6；BIOLOGICAL BUFFER TAPS

TAPS has a preparation method, you must not know
The full name of Taps: Trimethylol methylamino propanesulfonic acid, which belongs to a kind of biological buffer. The appearance is white crystalline powder, and it will have a pungent odor when volatilized in the air at room temperature. It has good water solubility. The average TAPS that can be dissolved per 100 grams of water reaches 50g, and it can also be dissolved in NaOH lye. The aqueous solution is colorless and transparent. The CAS number is 29915-38-6, the molecular formula is C7H17N06S, and the molecular weight is 243.28. Taps is also a dual ion buffer widely used in the fields of biochemistry and molecular biology, mainly used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits.

Preparation method of taps solution (about 1-2l)

(1) Prepare 0.1M solution (a): taps 24.328g/deionized water 1000ml

(2) Prepare 0.1M NaOH solution (b): NaOH 4G / deionized water 1000ml

At present, there are few reports on the synthesis process of TAPS, and the synthesis method of TAPS has been studied in detail based on online information. It is concluded that trimethylolaminomethane and 1,3-propane sultone are used as raw materials to react in an alcohol solvent.
Specific operation: Weigh equimolar amounts of Tris and 1,3-PS, and put an appropriate amount of alcoholic solvent into a 250ml three-necked flask with condensed reflux. Under stirring, heat up and reflux; after a period of reaction, Stop the reaction; at room temperature, let the reacted solution stand and cool to room temperature, and then filter under reduced pressure. The TAPS coarse filter cake is washed with an appropriate amount of absolute ethanol for 2 to 3 times. The TAPS filter cake is taken out and placed in a vacuum drying oven. Dry, and recover the filtrate filtered under reduced pressure for use. In the above synthesis process, the reaction time, different alcohol solvents and the recycling of reaction solvents all have a certain degree of influence on the yield and purity of TAPS. The experimental results show that the reaction time is controlled at 6 hours, and the yield is the highest. Continue to extend the reaction time, and the yield will decrease slightly; for the investigated reaction solvents of ethanol, n-propanol, isopropanol and n-butanol, n-butanol is used as the reaction solvent. TAPS has the highest yield and purity; in the reaction process, the recycling of the reaction solvent is beneficial to improve the yield and purity of TAPS. In summary, using Tris and 1,3-PS as raw materials to synthesize TAPS, the reaction time is controlled at 6 hours, and n-butanol is used as the solvent, and recycled during the reaction process, the yield and purity of TAPS obtained are the best.
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