The application of biological buffer BIS-TRIS in the detection of creatine kinase

In the field of medical diagnosis, enzyme detection has always been an important means of evaluating human health status. Among them, creatine kinase (CK), as an enzyme present in the myocardium, skeletal muscle, and smooth muscle, its activity level changes are often closely related to diseases such as muscle injury and myocardial infarction. Therefore, accurate and rapid detection of creatine kinase activity is of great significance for early detection and diagnosis of diseases.

BIS-TRIS powder

In recent years, with the continuous development of biochemical technology, a buffer called Bis (2-hydroxyethyl) amino (trimethyl) methane (BIS-TRIS buffer) has shown unique advantages in creatine kinase detection. During the detection of creatine kinase, BIS-TRIS can effectively maintain the acid-base balance of the reaction system, ensuring the smooth progress of enzymatic reactions. At the same time, the hydroxyl structure of BIS-TRIS also makes it have good hydrophilicity and biocompatibility, which can tightly bind with enzyme molecules, improve enzyme stability and activity.

The role of BIS-TRIS in creatine kinase detection

1. BIS-TRIS can provide a suitable pH environment. The activity of creatine kinase is greatly affected by pH value, and high or low pH can lead to a decrease or even inactivation of enzyme activity. BIS-TRIS, as a wide range buffering agent, can maintain stable buffering capacity under different pH conditions, providing a stable reaction environment for the detection of creatine kinase.

2. BIS-TRIS can improve the sensitivity and accuracy of detection. In the process of creatine kinase detection, BIS-TRIS can effectively reduce the impact of non-specific reactions and interfering substances, and improve the quality of detection signals. Meanwhile, BIS-TRIS can also promote the binding of enzymes to substrates, improve the efficiency of enzymatic reactions, and further enhance the sensitivity and accuracy of detection.

3. BIS-TRIS also has good stability and safety. During the detection of creatine kinase, BIS-TRIS is not easily affected by external factors such as temperature and light, and can maintain long-term stability. In addition, BIS-TRIS is non-toxic and harmless to the human body, making it suitable for clinical diagnosis and laboratory research.

4. BIS-TRIS also demonstrated good compatibility and scalability in creatine kinase detection. It can be combined with other reagents and detection methods to form a diverse detection system that meets the needs of different clinical and laboratory settings. For example, BIS-TRIS can be combined with techniques such as fluorescent probes and electrochemical sensors to achieve high sensitivity and specificity detection of creatine kinase.

Product packaging

In practical applications, when using BIS-TRIS for creatine kinase detection, it should be noted that different detection systems and experimental conditions may require different concentrations of BIS-TRIS. Therefore, when conducting creatine kinase detection, it is necessary to optimize the concentration of BIS-TRIS according to the specific situation in order to achieve good detection results. On the other hand, the activity of creatine kinase is greatly affected by time and temperature. Therefore, in order to obtain accurate detection results, it is necessary to strictly control the reaction time and temperature to ensure that the enzymatic reaction is carried out under good conditions.

Hubei Xindesheng Material Technology Co., Ltd. has a history of nearly 20 years in developing biological buffering agents. Currently, there are dozens of products on sale, and we have unique insights into the use and precautions of various biological buffering agents, ensuring that each batch of products can be used efficiently. If you are interested, click on the official website to learn more details!

FAQ

How do you make a Bis-Tris buffer?

To prepare 1 liter of 1M Bis-Tris buffer stock solution, dissolve 209.24 g of GoldBio Bis-Tris in 750 mL of dH2O. Adjust to desired pH using concentrated hydrochloric acid. A table is available for you to use in the Bis-Tris PDF protocol. Fill to a final volume of 1L with dH2O and sterilize by filter or autoclave.

What is the use of Bis-Tris?

BIS-TRIS is a popular gel, sample and running buffer for various types of electrophoresis. For instance, it has been used with ACES Buffer as a running buffer for gel electrophoresis (BIS-TRIS/ACES). Its use has also been reported in anion exchange chromatography, NMR spectroscopy and x-ray crystallography.