China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Proteins are essential molecules in life activities, and the study of their structure and function is of great significance for understanding biological processes, disease mechanisms, and drug development. In protein research, biological buffering agents are a crucial tool, especially playing an important role in protein purification and analysis processes. This article will explore the important applications of biological buffering agents in protein purification and analysis.   1、 Types and effects of biological buffering agents   Biological buffering agents are mainly used to maintain the acid-base balance of biological systems and prevent changes in protein structure and function caused by pH fluctuations. Common biological buffering agents include TRIS base, BICINE, HEPES, etc. These buffering agents can dissociate in solution, forming ion pairs, effectively stabilizing the pH value.   2、 Application of Biological Buffers in Protein Purification   In the process of protein purification, the role of biological buffering agents is mainly reflected in maintaining protein stability and regulating ion strength. On the one hand, the ions of the buffer can interact with the surface charges of proteins, affecting their solubility and stability. On the other hand, by adjusting the concentration of the buffer, the ion strength of the solution can be controlled, which helps with the separation and purification of proteins.   3、 The application of biological buffering agents in protein analysis   In the process of protein analysis, such as electrophoresis and mass spectrometry, biological buffering agents also play a crucial role. Firstly, in electrophoresis, a buffer can maintain a stable pH value in the electrophoresis tank, ensuring the smooth progress of electrophoresis. Secondly, in mass spectrometry analysis, buffering agents can prevent protein aggregation and precipitation, improving the sensitivity and accuracy of mass spectrometry analysis.   4、 Selection and use of biological buffering agents   Choosing the appropriate biological buffer is crucial for protein purification and analysis. Generally speaking, buffering agents that have little impact on protein stability and can maintain pH stability should be selected. At the same time, attention should also be paid to the ion strength and chemical properties of the buffer to avoid unnecessary interference with proteins.   5、 Conclusion   Biological buffering agents play an important role in protein purification and analysis. By selecting and using appropriate biological buffering agents, protein stability can be effectively maintained, ion strength can be regulated, and experimental efficiency and accuracy can be improved. With the deepening of protein research and technological progress, the application of biological buffering agents will become more extensive and important. At the same time, we also need to continue exploring and researching new types and properties of biological buffering agents to better serve the field of protein research.   In future research, further exploration can be conducted on how biological buffering agents affect the structure and function of proteins, as well as how to optimize the use of biological buffering agents to improve the efficiency and accuracy of protein purification and analysis. In addition, with the continuous development of biotechnology, new biological buffering agents and substitutes will continue to emerge, providing more choices and possibilities for protein research.   In summary, biological buffering agents are an indispensable and important tool in protein research. By gaining a deeper understanding of its mechanism of action and application methods, we can better utilize biological buffering agents to serve the field of protein research, promoting the development of biology, medicine, and pharmacy.   Desheng is a manufacturer of Hepes buffer with high purity and stable quality, which can be provided in large quantities. We can provide over 30 different types of buffer products, and if you need other biological buffer products, you can also purchase them in one stop. Feel free to contact us anytime if you need anything!

Ethylenediaminetetraacetic acid (EDTA) is a commonly used chelating agent, widely used in various fields, including medical examinations, chemical analysis, etc. And ethylenediaminetetraacetic acid tripotassium (EDTA K3) is the potassium salt form of ethylenediaminetetraacetic acid, which is used as an additive in EDTA tubes for blood anticoagulation in vacuum blood collection vessels. In addition to EDTA tripotassium, EDTA dipotassium and EDTA disodium may also be added to the EDTA tube.   The role of ethylenediaminetetraacetic acid tripotassium in blood collection:   Prevent blood clotting: Tripotassium ethylenediaminetetraacetate can chelate calcium ions in the blood, reducing the concentration of free calcium ions in the blood. When the concentration of calcium ions decreases, the difficulty of activating prothrombin increases, effectively preventing blood clotting.   Protecting blood components: In addition to preventing blood clotting, potassium ethylenediaminetetraacetate can also protect various components in the blood from damage. For example, it can protect the structure and function of blood cells such as red blood cells, white blood cells, and platelets, thereby ensuring the accuracy and reliability of blood samples. This is crucial for subsequent blood testing and analysis.   What tests can potassium ethylenediaminetetraacetate be used for   EDTA potassium anticoagulant tube, as one of the commonly used anticoagulant blood collection vessels in clinical testing, can provide accurate and reliable basis for various tests. Below, we will provide a detailed introduction to the application of EDTA potassium anticoagulant tubes.   1、 Blood cell analysis and blood routine testing   EDTA potassium anticoagulant tubes are commonly used tools for blood cell analysis and blood routine testing. In such testing, it is necessary to obtain a whole blood sample for counting and analyzing blood components such as red blood cells, white blood cells, platelets, etc. The presence of EDTA tripotassium is crucial for accurate measurement of blood cell counts, as it can provide accurate information on the number, morphology, and distribution of blood cells, providing strong support for clinical diagnosis and treatment.   2、 Platelet function testing   In addition to blood cell analysis and blood routine testing, EDTA potassium anticoagulant tubes are also widely used for platelet function testing. Platelets play an important role in hemostasis and thrombosis, and abnormal function may lead to bleeding or thrombotic diseases. EDTA potassium anticoagulant tubes can maintain the natural morphology and function of platelets, making the detection of platelet adhesion, aggregation, and release more accurate and reliable.   3、 Serum biochemical testing   EDTA potassium anticoagulant tubes can also be used for serum biochemical testing, such as the determination of liver function, kidney function, myocardial enzymes, amylase and other indicators. In these tests, serum isolation is required to remove cellular components and interfering substances from the blood. The anticoagulant effect of EDTA tripotassium can prevent blood coagulation and the formation of fibrin, which is beneficial for the separation and purification of serum. Meanwhile, it has a relatively small impact on enzyme activity and metabolites in the blood, ensuring the accuracy and reliability of biochemical indicators.   4、 Electrolyte detection   Electrolytes are essential substances for maintaining normal physiological functions in the human body, and their balance is crucial for human health. EDTA triple potassium anticoagulant tubes can be used for the detection of serum electrolytes, such as the determination of serum potassium, sodium, chloride, calcium, phosphorus and other indicators. In these tests, it is necessary to obtain serum samples without clots to ensure measurement accuracy. EDTA tripotassium can effectively prevent interference from blood coagulation and fibrinogen, providing clear serum samples for measurement with a power analyzer.   Desheng is a manufacturer of a complete set of blood collection additives, with nearly 20 years of experience in research and development and production of blood collection additives. More than ten types of blood collection additives can be provided. The product quality is stable, with small inter batch differences, and has been sold to multiple customers both domestically and internationally, receiving widespread praise. If you need anything, please feel free to call us for consultation and ordering!

EDTA-3K is a chemical substance also known as tripotassium ethylenediaminetetraacetic acid or 3K (EDTA). It is a white crystalline powder, odorless, easily soluble in water, and has stable performance. It has good chelating ability and can combine with cations such as calcium ions in the blood to form stable chelates, effectively controlling the concentration of calcium ions in the blood and preventing coagulation for a certain period of time. And EDTA-3K is also used in cosmetics as a moisturizer, stabilizer, etc. It helps to keep the skin hydrated and protect skin cells from free radical damage. At the same time, it is often used to prepare some special cosmetic additives, such as whitening agents.   （1） The reason why potassium ethylenediaminetetraacetate is used in cosmetics:   1. High safety: Tripotassium ethylenediaminetetraacetate is a safe and suitable chelating agent for cosmetics. It is considered non-toxic in many places and is therefore often added to various cosmetics.   2. Efficient chelation ability: Tripotassium ethylenediaminetetraacetate can effectively chelate metal ions, preventing their oxidation on cosmetics, thereby ensuring product quality and stability.   3. Good compatibility: Tripotassium ethylenediaminetetraacetate has good compatibility with other cosmetic ingredients, making it easier to use in combination with other ingredients during the formulation process, reducing production difficulty and cost.   4. Multiple functions: Tripotassium ethylenediaminetetraacetate has various functions, such as moisturizing and anticoagulating, so it can play an important role in the cosmetics field.   5. Good stability: The chemical properties of potassium ethylenediaminetetraacetate are stable, which enables it to play an important role in various application fields. Whether it is food processing, pharmaceutical preparation, or cosmetic production, it can maintain its effectiveness under harsh conditions.   Please note that although potassium ethylenediaminetetraacetate has many applications in cosmetics, its specific use must comply with relevant regulations, and the amount added must comply with relevant requirements and standards.   （2） Tripotassium ethylenediaminetetraacetate can be used as a variety of ingredients in cosmetics, such as moisturizers, antioxidants, anticoagulants, chelating agents, etc. The following are some types of cosmetics that may have added potassium ethylenediaminetetraacetate:   1. Skin care products: EDTA can be added to skin care products as humectant and antioxidant, such as face cream, lotion, essence, etc. It can enhance the moisturizing effect of the product while preventing the oxidation of metal ions in the product.   2. Cosmetics: EDTA can be used as stabilizer and humectant in cosmetics, such as lipstick, eye shadow, liquid foundation, etc. It can improve the stability of the product, extend its service life, and maintain the skin's hydration.   3. Shampoo and shower gel: Tripotassium ethylenediaminetetraacetate can serve as a chelating agent in shampoo and shower gel, removing metal ions from the skin and providing a cleansing effect. At the same time, it can also enhance the moisturizing effect of the product.   4. Anti aging products: EDTA can be used as antioxidant and humectant in anti-aging products, such as eye cream, facial essence, etc. It can effectively prevent the damage of free radicals to the skin while maintaining a hydrated feeling.   It should be noted that the amount of potassium ethylenediaminetetraacetate added to different cosmetics needs to comply with relevant requirements and standards to ensure the safety and effectiveness of the product. Meanwhile, for certain special groups of people, such as those with allergies or sensitive skin, skin testing should be conducted before using cosmetics with added potassium ethylenediaminetetraacetate.  

Serum separation gel is a special reagent used to separate serum or blood clots from blood samples, ensuring the accuracy of blood test results.   （1） The main components of serum separation gel   Including polysiloxane, silica, diluents, and additives. The reasonable ratio of these components enables the serum separation gel to have good thixotropic and isolation properties, and can maintain stability under various temperatures and environments. In addition, some serum separation gels also contain small amounts of silica powder and macromolecular hydrocarbons. The components of serum separation gel have high stability and temperature resistance, which can maintain stability in various temperatures and environments. At the same time, it also has good thixotropic and isolation properties, which can efficiently extract high-quality serum samples, improve the accuracy and reliability of experiments.   （2） The use of serum separation gel:   1. Used in experiments such as serum biochemistry, immunology, and drug detection, it can efficiently extract high-quality serum samples.   2.In blood testing, it is used to separate serum or plasma components from cellular components such as red blood cells, white blood cells, and platelets, reducing the interference of impurities such as cell debris, lipids, and fibrin on the test results.   3. In blood collection, it can simplify the blood processing process, avoid blood sample contamination of the operator, prevent virus infection in the blood, reduce medical waste, and improve work efficiency.   （3） Steps for using serum separation gel:   1. Add serum separation gel to test tubes at a dosage of 0.8-1.2g per tube.   2. Heat the serum separation gel at a temperature not exceeding 80 degrees Celsius. The recommended heating temperature is 40-60 degrees Celsius, and the heating time should be based on the uniform temperature of the separation gel gel.   3. Place the test tube with added serum separation gel on a centrifuge and centrifuge at 1500-2100g for 2-3 minutes.   4. When conducting a blood test, wait for the blood to completely coagulate and the serum to begin to precipitate. Leave the blood sample for 15-25 minutes, then centrifuge at 1500-2100g on a centrifuge for 8-10 minutes to separate the serum and blood cells.   （4） Precautions for using serum separation gel:   1. The amount of serum separation gel added should be accurate, as too much or too little can affect the separation effect.   2. The usage temperature of serum separation gel should not exceed 80 degrees Celsius, and the temperature should be controlled between 40-60 degrees Celsius during heating. The heating time should not be too long to prevent the gel temperature from being too high or the heating time from being too long and affecting the separation effect.   3. During the gluing process, colloidal contamination should be avoided to avoid affecting the separation effect.   4. During the centrifugation process, it is necessary to strictly control the centrifugation speed and time to avoid incomplete separation of serum and blood cells or impurities mixed in the serum due to excessive centrifugation speed or time.   5. During the operation, the blood collection tube should be kept upright to better discharge excess air from the serum separation gel and prevent the formation of bubbles.   6. Before use, ensure that the blood collection vessel is clean and dry to avoid affecting the separation effect.   7. The storage temperature of serum separation gel should be controlled within a certain range to avoid adverse factors such as direct sunlight and repeated freeze-thaw that may affect its performance.   Overall, serum separation gel, as an advanced blood separation technology, meets various detection needs, simplifies related operations, reduces error risks, and provides an efficient and reliable blood separation method for healthcare.   Serum separation gel has a wide range of applications in various fields, and the requirements for products are also very high. The manufacturer of serum separation gel needs sufficient experience and technology to ensure that the product can meet the standards for use under any conditions. Hubei Xindesheng Material Technology Co., Ltd., as a professional chemical reagent manufacturer, has always been committed to providing high-quality and high-purity chemical reagents, using advanced production equipment and complete production lines. At present, the company has a complete range of serum separation gel products for production and sale. If you are interested, please feel free to contact us for purchase at any time!

Serum separation gel is a polymer material with good thixotropic and isolation properties. It can form a network structure under the action of centrifugal force and re form a fluid with higher viscosity after the disappearance of centrifugal force. This thixotropy causes serum separation gel to form an isolation layer between serum and blood cells, and the specific gravity of serum separation gel is between serum and blood cells. Therefore, after blood centrifugation, it can form layered blood clots, separation gel, and serum. Effectively separate serum and blood cells. This makes serum separation gel play an important role in clinical testing.   In clinical testing, serum separation gel is mainly used to separate serum and blood cells, prevent material exchange between serum and blood cells, and ensure the accuracy of test results. By using serum separation gel, the operation process can be simplified, manual intervention and error rate can be reduced, and the accuracy and efficiency of detection can be improved.   The advantages of serum separation gel   1. Efficient separation: It can quickly and efficiently separate serum and blood cells, ensuring the accuracy of experimental results;   2. Maintaining blood purity: It can isolate the contact between serum and blood cells, prevent substances with higher content in blood cells from being released into the serum. If the substances exchange freely between them, it will interfere with the test results. The hydrophobic organic compound composition of serum separation gel gives it thixotropy, which can form an isolation layer between serum and blood cells to prevent material exchange and ensure the clarity of serum.   3. Improving experimental accuracy and reliability: This method can reduce the interference of impurities such as cell debris, lipids, and fibrin on the detection results, thereby improving the accuracy and reliability of the experiment;   4. Stable serum components: Ensure the stability of serum (plasma) components within a certain period of time;   5. Easy to operate: Traditional serum separation methods require multiple steps such as centrifugation, supernatant removal, and sample addition, making the operation cumbersome and prone to errors. To use serum separation gel, simply add the blood to the blood collection vessel containing serum separation gel, and then centrifuge to complete the separation process. This method of simplifying the operation process reduces manual intervention and error rates, and improves the accuracy and efficiency of detection.   6. Other advantages: Serum separation gel also has advantages such as antioxidation, high temperature resistance, low temperature resistance, and good airtightness.   Testing items for serum separation tubes   1. Biochemical testing: used to detect various biochemical indicators in the blood, such as blood sugar, blood lipids, liver function, kidney function, etc.   2. Immunological testing: used to detect various immune indicators, such as immunoglobulins, complement, autoantibodies, etc.   3. Tumor marker detection: used to detect the content of tumor markers, assisting in the diagnosis and monitoring of tumors.   4. Genetic testing: used to detect genes related to genetic mutations and hereditary diseases.   How to choose high-quality serum separation gel   The quality of serum separation gel can be determined through the following aspects:   1. Transparency: A high-quality serum separation gel should be in a transparent and impurity free state, where the separation layer of serum and blood clots can be clearly seen. If the serum separation gel is opaque or contains impurities, it may affect the separation effect and detection results.   2. Specific gravity: The specific gravity of serum separation gel should be between serum and blood cells, forming an isolation layer. Too light or too heavy a specific gravity can lead to ineffective separation of serum and blood cells.   3. Viscosity: The serum separation gel should have an appropriate viscosity to ensure the formation of a stable isolation layer during centrifugation. If the viscosity of the serum separation gel is too low, it may cause the isolation layer to be unstable and the serum and blood cells to mix together.   4. Stability: Serum separation gel should have good stability, maintain its physical properties unchanged for a certain period of time, and ensure the separation effect of serum and blood cells.   5. Safety: Serum separation gel should be non-toxic and harmless, and will not have a negative impact on blood samples and test results.   Desheng is a manufacturer of serum separation gel. Currently, the quality of the separation gel produced is stable and widely recognized by customers in the market. If you have any purchasing needs, please feel free to contact me or click on the official website to learn more details!

PIPES buffer, also known as piperazine-1,4-diethanesulfonic acid, is mainly used as a biological buffer and can be used in laboratory research and chemical production processes. It is a zwitterionic buffering agent with a pKa value of 6.76, which gives it good buffering performance within the physiological pH range (6.1-7.5). The traditional understanding of PIPES buffer is mainly used in fields such as cell culture, chromatography, diagnostic testing, etc. Today, let's take a look at its other hidden uses.   1、 PIPES is used in the ink field   Ink, as an important consumable in printing and dyeing technology, its quality and performance have a decisive impact on the printing and dyeing effect. Traditional dispersed dyes and organosilicon surfactant inks have some problems in the application process, such as reduced filling and spray stability, leading to poor image quality. To address these issues, a new type of ink composition has been developed, consisting of dispersed dyes, dispersed resins, and organosilicon surfactants. This ink combination has excellent performance and can meet various printing requirements.   Among them, PIPES buffer plays an important role in ink compositions. As a pH regulator, the main function of PIPES is to stabilize the acidity and alkalinity of ink, ensuring that it is within the appropriate pH range. At an appropriate pH value, ink is not prone to deterioration, sedimentation, or layering, thus maintaining good printing effects and performance. It can further optimize the quality and stability of ink, extend the shelf life of ink, and improve printing efficiency.   2、 PIPES in enzyme kinetics research   In enzyme kinetics research, enzyme activity is often affected by pH value. PIPES can serve as a reaction buffer, providing a stable pH environment, ensuring enzyme stability and activity, promoting substrate enzyme binding, and maintaining a suitable ion environment. Assist researchers in understanding the kinetic characteristics and mechanisms of enzymatic reactions, and reduce experimental errors.   3、 The application of PIEPS in fertilizers   As a pH regulator in fertilizers, PIPES can stabilize the acidity and alkalinity of fertilizer solutions, thereby optimizing the solubility of fertilizers and the absorption efficiency of plants. This regulatory effect has a significant impact on the solubility, stability, and nutrient absorption of fertilizers by plants. Adding PIPES in moderation can promote plant nutrient absorption and improve plant growth and development.   However, when using PIPES, attention should be paid to the rationality of its usage method and dosage. Prepared according to the type of fertilizer, soil conditions, and plant needs. At the same time, following the advice of fertilizer manufacturers or agricultural experts is also key to ensuring the effectiveness of fertilization. Reasonable use of PIPES can provide strong support for agricultural production.   At present, PIPES buffer has become an important experimental tool in many biological and chemical experiments. Its practical use is broader than described in this article. If you are interested, you can enter the official website of Desheng to inquire and learn more.   Hubei Xindesheng Material Technology Co., Ltd. is a manufacturer of PIPES buffering agents. The appearance of the products is white powder, and they can be configured as a solution when used. The purity of the products can reach over 99%. It has the advantages of good stability, wide temperature range, minimal impact on biological activity, and oxidation resistance. In addition, Desheng can also provide dozens of buffering agents such as TRIS, HEPES, CAPS, BICINE, etc., which can meet the usage needs of different customers. If you need to make a purchase, please feel free to contact us at any time. Looking forward to your call!

Heparin is a commonly used anticoagulant in clinical practice, widely used in blood tests and various medical procedures. Generally, it has two salt forms, namely sodium heparin and lithium heparin, both of which play an anticoagulant role. However, although their effects are similar, their binding ions and degree of dissociation are different. Heparin lithium exhibits better anticoagulant effects than heparin sodium in certain situations and is more suitable for certain blood test items. Let's take a look at the blood test items suitable for heparin lithium.   1、 Biochemical testing of patients after hemodialysis   For biochemical tests of hemodialysis patients, heparin lithium plays a significant role. For example, during hemodialysis, the dialysate usually contains heparin anticoagulants, such as low molecular weight heparin calcium. These anticoagulants can make blood samples less prone to clotting, making it difficult to separate samples and obtain plasma. Therefore, using heparin lithium anticoagulant plasma is an ideal choice for biochemical testing in dialysis patients. It not only effectively prevents blood clotting, but also helps to extract accurate plasma samples. According to reports, the accuracy of the results is high.   2、 Liver function examination   Blood samples treated with lithium heparin also have a special role in liver function tests, which usually involve the analysis of multiple blood parameters to evaluate liver health, effectively treat or prevent diseases. The use of heparin lithium tubes for examination, such as in the detection of liver diseases such as hepatitis, liver cancer, and fatty liver, helps to maintain blood flow, make the measurement of various blood parameters more accurate, and thus improve the efficiency of the examination work.   3、 Hemorheological testing   In the detection of cardiovascular and cerebrovascular diseases, the rheological properties of blood are very important. Some diseases, such as high viscosity, lead to abnormal increases in blood viscosity, which may increase the risk of thrombosis and cardiovascular disease. In these cases, hemorheological testing can help identify risk factors. Heparin lithium, as an anticoagulant, can be used to ensure that blood maintains fluidity during rheological testing, without causing inaccurate results due to coagulation, which is helpful for early diagnosis of cardiovascular and cerebrovascular diseases.   Lithium heparin is widely used in blood tests, and its superior anticoagulant performance makes it an ideal choice for medical staff. As a manufacturer of lithium heparin raw materials, Desheng can supply high-quality reagents of analytical grade. More than 100 domestic blood collection manufacturers have chosen to cooperate with us. If you have any relevant intentions, please feel free to contact us for purchase at any time!

In the modern medical field, the collection and analysis of blood samples are very common clinical operations, and blood testing is one of the important ways for doctors to diagnose and monitor the health status of patients. To ensure that the collected blood samples do not coagulate after collection and to ensure accurate experimental results, appropriate anticoagulants need to be used. And the heparin lithium collection tube is one of them, and its working principle and application will be introduced below.   1、 The working principle of heparin lithium   Heparin lithium is an anticoagulant reagent, usually prepared as a solution from powder and sprayed onto the inner wall of blood collection vessels. Its main function is to prevent blood from clotting after collection, ensuring that the blood sample remains in its original state for subsequent testing. The anticoagulant mechanism is mainly due to the fact that heparin lithium can bind with antithrombin III (AT-III) to form the heparin AT-III complex. AT-III is a naturally occurring protease inhibitor that can reduce the activity of coagulation factors such as Xa and IIa. By binding to AT-III, coagulation factors are inactivated, thereby inhibiting the formation of prothrombin kinase and further preventing blood clotting.   2、 Application of heparin lithium collection tube   Lithium heparin collection tubes have a wide range of applications in medical laboratories and clinical practice. Firstly, it is commonly used in biochemical tests to evaluate liver function, kidney function, glucose metabolism, lipid metabolism, etc. It can ensure that blood samples remain stable after collection, thereby providing reliable detection results. In addition, in coagulation tests, it is necessary to analyze coagulation factors in the plasma, such as PT (prothrombin time) and APTT (activated partial coagulation time). The heparin lithium collection tube can ensure the stability of the plasma and is suitable for these tests.   3、 Precautions for using heparin lithium collection tubes   To ensure sufficient anticoagulation of the blood in the collection tube, the blood sample should be reversed and mixed 5-8 times as soon as possible after collection, especially when the blood collection environment is above 25 degrees Celsius. Effective contact between the blood and heparin lithium must be ensured, otherwise local coagulation and non coagulation may occur, which is not conducive to subsequent testing. In addition, as heparin anticoagulation is not irreversible, the collection tube should be tested within 6 hours after blood collection and should not be left idle for a long time, which may cause accidents.   Overall, the use of heparin lithium collection tubes in medical laboratories ensures the accuracy and reliability of blood samples, helping medical staff to make accurate diagnoses and treatments. This anticoagulant is also recognized as an ideal anticoagulant in the medical field. Note that when using heparin lithium collection tubes, it is necessary to follow standard blood collection procedures and operating procedures to ensure the quality of the collected samples.   As a supplier of blood vessel additives, Desheng can provide high-quality raw material powders and has the ability to provide long-term supply and improve after-sales service. It has high market recognition and many repeat customers. If you are interested, please feel free to contact us for purchase at any time!

Recently, our company has achieved significant results in scientific and technological innovation, with a total of 5 scientific and technological achievements winning the 2023 Hubei Province Science and Technology Achievement Registration Certificate. These achievements have reached the leading level in terms of technological content, innovation, and practicality in China, injecting new impetus into the company's development.   The Science and Technology Achievement Registration Certificate is a legally effective technical certification document issued by the science and technology management department. It can serve as an important basis for science and technology achievement registration, new product tax incentives, science and technology awards, science and technology plan approval, production license approval, technology transfer, science and technology achievement promotion, asset evaluation, and intellectual property rights. The 5 registration certificates for scientific and technological achievements that our company has won this time fully demonstrate our strength and achievements in scientific and technological innovation and research and development.   1、 "A method for preparing low density serum separation gel using a microchannel reactor"   This technological achievement was carefully researched and completed by our R&D team, with the main members including Wen Heng, Hu Huidi, Wang Anqi, and Wang Zhongxi. They have made significant breakthroughs in the preparation of microchannel reactors, successfully developing an efficient and reliable method for preparing low density serum separation gel. This method uses a microchannel reactor and achieves efficient preparation of low density serum separation gel by optimizing reaction conditions. The highlight of this technology lies in improving the stability of the separation gel, providing a more reliable experimental tool for the biomedical field.   2、 "A method for synthesizing luminol or isoluminol using a one pot method"   Another noteworthy technological achievement was completed by Wang Zhongxi, Wang Anqi, Xiong Fei, and Liu Jie. They have successfully developed a new method for synthesizing luminol or isoluminol using a one pot method, providing a more convenient preparation pathway for the field of photochemiluminescence. This innovative method not only simplifies the synthesis steps, improves synthesis efficiency, but also reduces waste generation during the synthesis process, which is in line with the development concept of green production. This has a positive significance for the widespread application of luminol and isoluminol in chemiluminescence.   3、 "A N-ethylation catalyst and its application in the preparation of N-ethylaniline derivatives"   The technological achievement jointly completed by team members such as Wang Zhongxi, Liu Jie, and Wang Anqi involves the development of N-ethylation catalysts and their application in the preparation of N-ethylaniline derivatives. This catalyst has the advantages of high efficiency and selectivity in the synthesis of N-ethylaniline derivatives, providing a new and feasible synthesis path, and has a positive promoting effect on research and production in related fields.   4、 "An opaque polymer resin and its application in blood separation gel"   This achievement involves the development of opaque polymer resin and its application in blood separation gel. The introduction of opaque polymer resin makes the separation gel more transparent and stable, improving the effectiveness in the blood separation process. This provides new material choices for the research and production of related medical equipment.   5、 A Carbomer and Its Preparation Method   This mainly involves the study of carbomer and its preparation methods. Carbomer has a wide range of applications in the field of daily chemical products, and the innovative preparation method of this technological achievement provides a new way for the efficient synthesis of carbomer, which has important economic and social value.   Behind obtaining the 2023 Hubei Province Science and Technology Achievement Registration Certificate is the hard work and continuous pursuit of scientific and technological innovation of our technology team over the years. The certification of these technological achievements will further stimulate our enthusiasm in the field of technological innovation, laying a solid foundation for the company's future development. We will continue to uphold the concept of innovation, continuously promote the transformation and application of scientific and technological achievements, and provide better services to society and customers.

In the medical field, blood testing is an important means of diagnosing and treating various diseases. To ensure the accuracy and reliability of blood testing, experimental tools are crucial. Heparin sodium anticoagulant blood collection with a green cap is a commonly used medical consumable, mainly used for collecting blood samples from patients. Its unique green cap design makes the blood collection vessel easy to identify, making it convenient for medical staff to use correctly during the blood collection process. Heparin sodium, as an anticoagulant, can effectively prevent blood clotting and ensure the fluidity of blood samples, which is crucial for subsequent blood testing.   Introduction to heparin sodium anticoagulant blood collection   Heparin sodium anticoagulant is present in blood collection vessels. It is a naturally occurring substance with strong anticoagulant effects. By binding with antithrombin, it inhibits its activity and prevents blood clotting. Widely used in clinical laboratory testing. In addition, other additives such as anti irradiation serum separation gel, blood coagulant, EDTA, etc. can be added to other colored blood collection vessels to optimize the separation and collection of blood samples.   The application of heparin sodium for anticoagulation and blood collection   Heparin sodium anticoagulant blood collection is mainly used to collect blood samples from patients and perform blood pre-treatment. The processed samples can be used for clinical testing. These testing items include blood routine, biochemical testing, immune testing, etc. Through these tests, doctors can understand the patient's physiological status, diagnose the disease, and monitor treatment effectiveness.   Precautions for using heparin for anticoagulation and blood collection   When using heparin sodium for anticoagulation and blood collection, the following points should be noted:   1. Ensure that the blood collection vessel is not damaged or leaking to prevent blood leakage;   2. The correct blood collection process should be followed, including steps such as disinfection, puncture, and blood collection;   3.During the blood collection process, excessive compression or force should be avoided to avoid affecting the quality of the blood sample;   4.In addition, the collected blood samples should be sent to the laboratory for testing as soon as possible to avoid prolonged storage that may cause blood coagulation or deterioration;   5. When transporting blood samples, it is important to ensure that the blood collection vessel is placed steadily and avoid shaking or inversion to avoid affecting the test results.   The advantages of heparin sodium anticoagulation for blood collection   1. Protecting blood samples: Heparin sodium has anticoagulant, antioxidant, and protective effects on bioactive substances in blood samples, which can prolong the storage time of blood samples.   2. Easy to use: The use of heparin sodium anticoagulant blood collection simplifies the clinical testing process, improves detection efficiency, and provides strong support for diagnosis and treatment;   3. Good biocompatibility: Heparin sodium is a naturally occurring substance with good biocompatibility, which can reduce adverse reactions between blood collection vessels and blood;   4. Safety: When used at normal doses, heparin sodium has a lower incidence of adverse reactions and higher safety.   Hubei Xindesheng Material Technology Co., Ltd. is a manufacturer of blood sampling additives. Desheng has accumulated rich knowledge and experience in blood sample pretreatment and has a professional technical service team. The main products for pre-treatment of blood samples include heparin sodium, heparin lithium, serum separation gel, EDTA dipotassium, tripotassium, disodium, etc. The product quality and technology have been widely recognized by customers both domestically and internationally. If you need to make a purchase, please feel free to call us for consultation and ordering. Looking forward to your call!

Biological buffer PIPES powder is a chemical reagent widely used in biological experiments, and its full name is piperazine-1,4-diethanesulfonic acid. As a biological buffer, PIPES solution plays an important role in maintaining the stability of the intracellular environment and protecting cells from pH fluctuations. This article will provide a detailed introduction to the properties, uses, and preparation methods of the biological buffering agent PIPES powder.   PIPES is a white powdered chemical reagent with the chemical formula C8H12N2O4S and a molecular weight of 248.26. It is a zwitterionic buffering agent with stable pH buffering ability, capable of maintaining a certain pH value in acidic or alkaline environments. In addition, PIPES also has high ion strength and low permeability, making it widely used in biological experiments such as cell culture, electrophoresis, and immunoblotting.   The application of biological buffer PIPES in biological experiments   PIPES powder, as a biological buffer, has been widely used in many biological experiments. The following are several typical application areas:   1. Cell culture: In cell culture experiments, PIPES can serve as a pH buffer to maintain the pH stability of the culture medium and ensure normal cell growth and division. It can serve as an ion carrier to facilitate ion exchange inside and outside the cell, affecting the physiological functions of the cell.   2. Electrophoresis: In electrophoresis experiments, PIPES powder can be used as a molecular sieve and ion exchanger to improve the resolution and effectiveness of the experiment. For example, in protein electrophoresis experiments, PIPES can bind to proteins, slowing down their movement in an electric field, thereby improving the resolution of the experiment   3. Immunoblotting: In the immunoblotting experiment, PIPES can be used as the main component of the transmembrane buffer to help transfer proteins from the gel to the membrane. Meanwhile, PIPES can also serve as an ion carrier to improve the sensitivity and specificity of experiments.   4. Purification of microtubule proteins: In microtubule protein purification experiments, PIPES can serve as the main component of the buffer to assist in the extraction and purification of microtubule proteins from tissues. It can also inhibit the polymerization reaction of microtubule proteins and improve purification efficiency.   5. Peroxisome localization: In peroxisome localization experiments, PIPES can serve as the main component of the buffer to assist in the ultrastructural localization of peroxisomes in cells. It can also protect cells from damage by peroxisomes.   In addition to the aforementioned application areas, PIPES can also be applied in biological experiments such as the separation and purification of nucleic acids and proteins, protein crystallization, tissue culture, and drug screening. In summary, as an important biological buffer, it plays an indispensable role in many biological experiments.   Preparation method of biological buffer PIPES   1. Prepare the required reagents and equipment   PIPES powder, ultrapure water, glass bottle, weighing paper, electronic balance, magnetic stirrer or vortex oscillator.   2. Weighing PIPES powder   Accurately weigh PIPES powder using an electronic balance based on the required concentration and volume. For example, if 100 mL of 0.25 mol/L PIPES solution needs to be prepared, 25.7g of PIPES powder needs to be weighed.   3. Dissolve PIPES powder   Add the weighed PIPES powder into a glass bottle, add an appropriate amount of ultrapure water, and then stir with a magnetic stirrer or vortex oscillator until dissolved. Note that sufficient ultrapure water should be used to ensure complete dissolution.   4. Filtering and sterilization   Filter and sterilize the prepared PIPES solution to remove impurities and bacteria from the solution. Disposable syringes or filters can be used for filtration.   5. Storage and Use   Store the filtered PIPES solution in a clean glass bottle and store it in a refrigerator at 4 ℃. When using, an appropriate amount of PIPES solution should be added according to experimental requirements.   Precautions for the use of biological buffer PIPES   1. PIPES powder has hygroscopicity, so it should be stored in a dry environment to avoid moisture;   2. During the configuration process, ultrapure water or deionized water should be used to ensure the purity and quality of the solution;   3. The prepared PIPES solution should be a colorless and transparent liquid. If turbidity or precipitation occurs, it may be due to incomplete dissolution of the powder or the presence of impurities, and it should be reconfigured;   4. The prepared solution should be used up within the specified time to avoid prolonged storage that may affect the quality and effectiveness of the solution.   Desheng is a manufacturer of PIPES powder with high purity and stable quality, which can be provided in large quantities. We can provide over 30 different types of buffer products, and if you need other biological buffer, you can also purchase them in one stop. Feel free to contact us anytime if you need anything!  

The collection and processing of blood samples are crucial in clinical laboratory examinations. Among them, procoagulant blood collection is often used for coagulation tests and early diagnostic experiments for some diseases. However, hemolysis may occur when using blood collection vessels containing coagulants. Hemolysis is a complex process that can affect the accuracy and reliability of experimental results. This article will explore the reasons for hemolysis in blood collection using coagulants.   The components and effects of coagulants in blood collection   Coagulant agents usually contain coagulant powder (a white powder substance that needs to be prepared as a solution when used) or coagulant suspension (a milky white suspension that can be directly sprayed for use) in blood collection. The function of these two reagents is to accelerate the coagulation process of blood, causing it to quickly form clots in the blood collection vessel. This component has no effect on clinical biochemical testing and can facilitate subsequent experimental analysis.   The causes of hemolysis phenomenon   Hemolysis refers to the process in which red blood cells rupture and release intracellular substances during the preparation process after blood samples are collected from blood collection vessels. This may affect the experimental results, especially for experiments that require detection of intracellular components in red blood cells. There are various reasons for hemolysis during the use of coagulant blood collection vessels. Below, we will explore in detail some of the main reasons.   1. Improper blood collection   During the blood collection process, if the operation is improper or the blood collection technique is not proficient, it may cause damage to the blood vessel wall, leading to hemolysis. In addition, excessive compression or forceful shaking of the blood collection tube may also cause red blood cell rupture.   2. The stimulating effect of coagulants   Some coagulants may have a stimulating effect on red blood cells, leading to their rupture. When choosing products, it is important to purchase from reputable manufacturers or channels. Ensure reliable product quality.   3. Transportation and storage conditions   Improper transportation and storage conditions may cause shaking or temperature changes in the blood collection vessel, leading to hemolysis. For example, long-distance transportation or extreme temperature environments may cause the components inside the blood collection vessel to separate or produce bubbles, leading to the rupture of red blood cells.   4. Improper specimen handling   During specimen processing, if the mixing time is too long and the force is too strong, it may damage red blood cells and cause hemolysis. In addition, if the blood collection tube is not evenly filled and applied before use, it may also lead to some blood samples not fully contacting the coagulant, forming clots, while other parts continue to undergo hemolysis.   Strategies and methods to reduce hemolysis   In response to the possible causes of hemolysis mentioned above, the following strategies and methods are provided to reduce the occurrence of hemolysis:   1. Improve blood collection techniques   Strengthen training and technical guidance for blood collection personnel to enable them to master correct blood collection techniques and methods. Meanwhile, during the blood collection process, it should be kept gentle and stable, avoiding excessive force or compression of the blood collection needle.   2. Choose the appropriate coagulant   Select appropriate coagulants for different experimental needs and ensure their quality. Hubei Xindesheng Material Technology Co., Ltd. is an old brand manufacturer of blood collection additives. Their coagulants have stable quality, good usage effects, and can quickly promote blood coagulation, making them the best choice for blood collection manufacturers. It is also worth noting that the expiration date of the coagulant should be regularly checked to avoid using expired products.   3. Optimize transportation and storage conditions   Ensure that the blood collection vessel remains stable during transportation and storage, avoiding violent shaking or temperature fluctuations. For long-distance transportation of blood collection vessels, necessary shockproof and insulation measures should be taken.   4. Standardize specimen processing procedures   When handling blood samples, standardized procedures should be followed. For example, the shaking or stirring time should not be too long, and the force should not be too strong; The blood collection tube should be thoroughly shaken before use. In addition, for specimens suspected of hemolysis, timely evaluation and treatment should be carried out.   Hubei Xindesheng Material Technology Co., Ltd. was established in 2005 and is a production-oriented enterprise specializing in the research and development, production, and sales of blood testing reagents. We can provide a complete set of blood collection additive products to meet the different usage needs of customers. Stable quality and high purity, please feel free to call us for inquiries and orders if needed.