China Wuhan Desheng Biochemical Technology Co., Ltd Company News

First, let's understand the basic concept of anticoagulation Anticoagulation: physical or chemical methods are used to remove or inhibit some coagulation factors in the blood and prevent blood coagulation. It is called the upper pale yellow liquid after anticoagulation centrifugal separation as plasma. Anticoagulant: chemical agent or substance that can prevent blood from clotting. It is called anticoagulant or anticoagulant. Coagulation: the process of helping blood clot quickly. Coagulant: substance that helps blood clot rapidly to precipitate out of serum. The general components are gum substances. In serum test, coagulant can be selected to make blood coagulate rapidly, while in whole blood or plasma test, different anticoagulants are usually needed to make the collected venous blood not coagulate. Anticoagulants account for a large proportion in medical examination, so what are the commonly used anticoagulants? I. EDTA (ethylenediaminetetraacetate) 1. Anticoagulation principle of EDTA: it can form chelate with calcium ion in blood, so as to prevent blood coagulation. Such as EDTA-K2, etc. 2. Blood collection: Purple head tube. 3. Application: commonly used and blood routine examination, blood cell morphology examination, etc. Two, heparin 1. Common heparin products include heparin lithium and heparin sodium. Anticoagulant principle: widely exists in liver, lung and other human tissues, is a physiological anticoagulant. By strengthening the activity of antithrombin Ⅲ (at - Ⅲ), the serine protease is inactivated and the formation of thrombin is prevented, so as to play an anticoagulant role. Such as heparin lithium, heparin sodium, etc. 2. Blood collection: green head tube. 3. Application: it is commonly used in biochemical tests. III. oxalate 1. Anticoagulation principle: oxalate reacts with calcium ion to form calcium oxalate precipitate, so as to prevent blood coagulation. Such as sodium oxalate, ammonium oxalate, etc. 2. Blood collection: gray head tube. 3. Application: it is often mixed with sodium fluoride (which can inhibit glucose fermentation) for blood glucose determination. Since 2005, Desheng technology has developed and produced in vitro diagnostic reagents, such as separation gel, heparin, EDTA potassium salt, etc. it has a deep research on new Trinder's reagents, bicine, Tris and luminol, acridine ester and other biobuffers such as chromogenic substrate TOS, Maos, etc., and has a professional advantage in independent research and development and synthesis.

The market share of in vitro diagnostic reagents in China can reach about 70%. Domestic in vitro diagnostic reagents are relatively mature. After years of development, China's independent innovation ability in the field of in vitro diagnostic reagents has been significantly improved, and the overall technical level has basically reached the international level at the same time. There are more than 200 kinds of in vitro diagnostic reagents. The main products on the market are liver function, kidney function, blood lipid, special protein, etc.                                                             Clinical biochemistry is based on the normal biochemistry of human body to study the biochemical changes in pathological state. By analyzing the changes of related metabolites, we can find the characteristic markers and establish the corresponding detection methods, so as to provide biochemical information and decision-making basis for disease prevention, diagnosis, treatment and prognosis. In the application of in vitro diagnosis, biochemical diagnosis is a commonly used diagnostic method. Biochemical diagnosis refers to the in vitro diagnostic method which uses the Lamber Beer law to determine various inorganic elements, protein and non protein nitrogen, enzyme, sugar, lipid and other biochemical indexes in vitro through various biochemical reactions. Our commonly used inspection items of liver function, renal function, blood glucose, blood lipid and so on belong to Biochemical diagnosis. Clinical biochemical diagnostic reagents are used in combination with biochemical analyzer to diagnose the relevant clinical biochemical indexes of human body by applying the principles of chemistry, enzymology, immunology and other related technologies. Through the specific reaction between reagents and related substances to be tested, specific optical signals are given, recorded by biochemical instrument, and compared with calibrator to give the level of related substances to be tested. Biochemical diagnosis is one of the most commonly used in vitro diagnosis methods, and it is also the earliest and most mature IVD subdivision field at home and abroad. In the 1990s, a large number of in vitro diagnostic reagent manufacturers and import agents have sprung up. With the increase of production enterprises and the fierce increase of the demand for commercial reagents in clinical tests, Desheng technology in vitro diagnostic business has also got the opportunity of rapid development, rapid growth and rapid development. Biochemical production line is increasingly rich and mature. In the 21st century, China's in vitro diagnostic reagent industry has entered a mature stage. In today's biochemical reagent production enterprises, a large number of national and excellent enterprises that can be compared with international famous manufacturers emerge, such as: Wuhan Desheng, Beijing Jiuqiang, Beijing Lidman, Shanghai Kehua, Ningbo Meikang, etc. In recent decades, the biochemical diagnosis industry in China has started from scratch and is growing and improving. At present, it has gone out of the country to the world.

Anticoagulation mechanism of EDTA-K2   EDTA-K2 is one of the most commonly used and important anticoagulants and reagents in the clinical work. Its mechanism is to prevent blood coagulation by forming stable chelates with calcium ions in the water phase. The salts of EDTA include potassium, sodium, lithium salt, which are all soluble in water. The solubility of potassium salt is higher than that of sodium salt. It is better to use potassium salt of EDTA for whole blood cell count. EDTA can affect the activity of some enzymes and inhibit lupus erythematosus factor, so it is not suitable for making histochemical staining and blood smear to examine lupus erythematosus cells. EDTA can also affect platelet aggregation and leukocyte phagocytosis, and is not suitable for hemostatic test and platelet function test. The smear prepared with EDTA-K2 anticoagulant has an effect on the morphology of granulocytes, the degree of which is related to the time of anticoagulant placement and the concentration of   anticoagulant.                                                                             Anticoagulation mechanism of heparin sodium Heparin is the best anticoagulant in the determination of blood chemical composition. Heparin is a kind of mucopolysaccharide with sulphuric acid group, with molecular weight of 15000. Its anticoagulation mechanism is to work with anticoagulant II to inhibit the action of factors ⅸ a, Ⅷ and PF3 in low concentration, and to strengthen the action of anticoagulant III to inactivate serine protease, so as to prevent the formation of thrombin; it also has the effect of inhibiting the self catalysis of thrombin and inhibiting factor X. The salts of heparin are sodium, lithium and ammonium. Generally, the anticoagulant dosage of heparin is 10.0-12.5iu/ml blood. Although the price of heparin lithium is expensive, its anticoagulation effect is better. Because heparin sodium can increase the content of plasma sodium, while heparin ammonium can increase the content of urea ammonia. There are obvious differences in some biochemical components between serum and heparin anticoagulant plasma. In the process of coagulation, the content of serum potassium is higher than that of plasma due to the dissolution and destruction of red blood cells. Therefore, when determining potassium ion, pay attention to the difference between serum and plasma. In addition, heparin excess can cause leukocyte aggregation and thrombocytopenia, so it is not suitable for leukocyte classification and platelet count, nor for hemostatic test. The results showed that heparin sodium anticoagulant had a significant effect on the determination of total protein in blood, which was 3% - 5% higher than that in serum. However, after adding anticoagulant, the total protein content is lower than the total protein content of serum. The reason is that after adding heparin sodium anticoagulant to blood, the production of thrombin is blocked, the blood coagulation is prevented, the fibrinogen cannot be hydrolyzed into fibrin monomer, and the formation of fibrin monomer is effectively blocked, and these fibrin remains in plasma. Serum is the blood after coagulation, fibrinogen hydrolysis into fibrin monomer, and because of coagulation and consumption, after centrifugation with the blood cells separated. Therefore, the total protein quality in plasma is all the proteins including fibrinogen, while the total protein content in serum is no fibrinogen. Theoretically, it should be lower than the total protein content in plasma. Therefore, when determining the total protein content of blood, it's better to take serum as the sample. For some emergency samples, or some patients who need to be determined urgently for other reasons, when it's necessary to use plasma as the sample to measure the total protein, after measuring the total protein, the content of fibrinogen should be deducted (generally 3% - 5%), so as to be consistent with the total protein content measured by taking serum as the sample, It truly reflects the actual total protein content in patients. The quality of EDTA 2k and heparin sodium produced by Desheng technology can fully compete with the imported enterprises. Our products have established long-term cooperative relations with many enterprises. Some customers order 100 kg of EDTA 2kg of potassium in Desheng technology every month. We believe that good products and good services will surely bring more long-term customers, and that customer-oriented, symbiotic and win-win situation is the common goal of Desheng and all major enterprises.