China Wuhan Desheng Biochemical Technology Co., Ltd Company News

At present, due to the continuous epidemic of foreign epidemics, most imported raw materials are restricted, but the related demand is very large. Carbomer 940 is one of them. Many companies in the market are looking for products, and the quality of domestic production is also uneven, so how to judge the quality of carbomer products?   Carbomer 940 is a very common product of Carbomer series. It has good thickening, high transparency, high viscosity and strong suspending ability. It is widely used in skin care lotions, creams, It is used as emulsifier, suspending agent, thickener and gelling agent in transparent gel products. Due to the important role of carbomer in daily chemical products and the extreme shortage of imported raw materials, many domestic manufacturers began to produce carbomer products, but not every product can meet the demand. Refer to the following methods to easily determine the quality of Carbomer 940 products:   1. From the appearance, high-quality carbomer should be white loose powder, or slightly yellowish, if the color is darker, it means that the product is not pure enough to contain color impurities. 2. In terms of hand feel, carbomer 940 should be lighter and looser. Because its molecules are highly polymerized long chains, the molecules are looser. If the product powder is loose and relatively compact, it means that acrylic acid polymerization control Poor, uneven degree of polymerization or different molecular size, etc .; this way the product is relatively poor in shear resistance, gel transparency and thickening. 3. In terms of odor, since carbomer itself is polyacrylic acid or polycarboxyvinyl, containing acidic carboxyl groups, the polymer is also not volatile, and only has a weak acid odor; if the taste is heavier or has other odors, it may be produced Solvent remains during the drying process. 4. From the transparency of the gel, after dissolving with water or ethanol, the gel formed should be transparent and clear, and the gel is uniform. If the gel is turbid and the light transmission is poor, it will seriously affect the use.   The above is the initial method of judging the quality of Carbomer 940 based on the experience of the production and sales process of Desheng. However, in the process of reagent R&D, it is still necessary to make the final judgment through testing and performance in specific products; In addition to the advantages of synthetic technology, our company also strictly conforms to the ISO9001 management system in the production management of the Carbomer series, which also guarantees the long-term stability of product quality!

Carbomer 940 is a polyacrylic acid polymer. The compound usually appears as a white powder with a slight odor. Properties: (jelly type) very short rheology, high transparency, low shear resistance, high yield value (suspension capacity) ), Can be used as thickener and emulsion stabilizer. It is known for being widely used in the cosmetics industry. Widely used in the pharmaceutical industry, personal care and cosmetics industries. The largest proportion is used for personal care and cosmetics, the ratio is about 54%.   Application of carbomer 940 in skin care emulsion Carbomer 940 has the functions of thickening, suspending, stabilizing the system, regulating the release of water and active substances, and has a simple process and good stability. Therefore, it is widely used in skin care emulsions. It is more refreshing because it has these two advantages, making it more popular with skin care lotions and gel products. Most people often pay attention to the translucent effect when choosing skin care products. Carbomer 940 greatly satisfies many people. In this regard, in addition, the skin care lotion it makes is relatively refreshing and very easy to apply. This has become the first choice for many people with greasy skin, and its status in the skin care field cannot be underestimated.   Application of carbomer 940 in pharmacy The application of Carbomer 940 in pharmacy is mainly manifested in the application of gel. It can be applied to all kinds of oral gel and dental gel, and can be developed into a compound gel preparation which is suitable for consistency and has no greasy feeling and easy to smear. This preparation is effective in treating periodontitis, gingivitis and oral mucosal ulcers, and it has a faster effect and can last for a long time.Carbomer 940 gel matrix has good film forming and adhesion. Adding protein coagulant containing formaldehyde, thymol and other desensitizing drugs to the gel matrix can prolong the residence time of the drug in the teeth. Desensitization effect is enhanced.   Nowadays, the epidemic situation of new coronavirus is getting more and more serious, and all industries are deeply affected by the epidemic situation, resulting in our company's original business not being able to develop normally. Production of virus preservation solution, Carbomer 940, Carbomer 980 and other products have obtained a large number of orders as soon as they are on the market. Due to their wide use, they can be used in many fields. Warmly welcome to come to discuss cooperation!

Carbomer 980 and 940 are another polymerized acrylate material newly developed by the company after acrylate separation gel. Because of its good thickening, emulsifying, suspending and good transparency, it is widely used in skin care milk In cosmetics such as cream, hand-washing gel, sunscreen, non-alcohol perfume, hair conditioner, etc.   Carbomer is a white loose powder, slightly acidic, soluble in water to form a gel, and can produce a high-efficiency thickening effect at a very low dosage (conventional dosage 0.2-1%), thus preparing a very good emulsions, creams, gels and transdermal preparations with a wide viscosity range and different rheological properties; the pH is usually 2.5-4.0 according to the concentration, and it is usually necessary to add a neutralizing agent to adjust the pH to near neutral. Different carbomers have slightly different properties. Compared with 940, carbomer 980 has larger polymer molecules. Under different conditions such as concentration, temperature, pH, etc., its viscosity changes and rheological changes behave differently, each of them has different ionic resistance and shear resistance.     In addition to the above physical and chemical differences, Carbomer 980 and 940 also have differences in application, such as in skin care lotions or gels: 1. Carbomer 980 behaves in addition to elasticity under dynamic vibration of stress, carbomer 940 is worse but easier to apply evenly. 2. When the presence or concentration of ions increases, the transparency of carbomer 980 and 940 is very small, but the resistance of carbomer 980 to ions is higher than that of carbomer 940, so carbomer 980 can provide a relatively higher viscosity and yield value when the ions increase. 3. The skin feel caused by different carbomers is different. Carbomer 980 has a slight elasticity when it is initially applied and the skin feels moisturized and smooth. It is suitable for medium or partial formulas that feel rich, and it is not sticky after use; It feels clear and suitable for refreshing formula.   Desheng Technology has 15 years of experience in the research and development and production of raw materials for acrylic polymer systems. The supply of carbomers 980 and 940 is increasing with each passing day, and the blood collection tube separation gel is also exported to many overseas countries. The rich experience in biochemical reagent synthesis research and development has made its own contribution to the process of replacing imported reagents with excellent domestic reagents.

Carbopol, also known as carbomer, is an acrylic cross-linked resin obtained by cross-linking acrylic acid with pentaerythritol and the like. It is a very important rheology modifier. Carbopol after neutralization is an excellent gel matrix with thickening and suspension and other important uses. The process is simple and its stability is good. It is widely used in emulsions, creams and gels.   Carbomer is a white loose powder; it has a characteristic slight odor; it has hygroscopicity and is a medicinal adjuvant. Carbomer is a high molecular polymer of acrylic acid bonded allyl sucrose or pentaerythritol allyl ether. Calculated on dry goods, containing carboxylic acid group (-COOH) should be 56.0% ~ 68.0%. Carbomer discolors with m-diphenol, and is incompatible with phenol, cationic polymers, strong acids, and high-concentration electrolytes. Carbomer's trade name is carbopol, which is divided into 934, 940, 941, etc. according to viscosity.   Carbomer classification: Carbopol 940: short rheology, high viscosity, high definition clarity, low ion resistance and shear resistance, suitable for gels and creams. Carbopol 941: long rheology, low viscosity, high-definition clarity, medium ion resistance and shear resistance, suitable for gels and emulsions. Carbopol 934: cross-linked polyacrylic resin, local delivery system, stable at high viscosity, used in concentrated gels, emulsions, suspensions. Carbopol 1342: cross-linked polyacrylic resin, local drug delivery system, excellent rheology modifier in the presence of electrolyte, polymerization emulsification. Carbopol 980: cross-linked polyacrylic resin, topical delivery system, crystal clear gel, water or alcohol solvent. Carbopol ETD 2020: acrylate/C10-30 alkyl acrylate cross-linked copolymer, long rheology, low viscosity, high-definition clarity, high ionic resistance and shear resistance, suitable for clear gels. Carbopol AQUA SF-1: liquid, long-rheological, can be formulated into clear formula, has excellent compatibility with various ingredients, acid thickening, can be used in surfactant system. carbopol Ultrez 21: acrylate/C10-30 alkyl acrylate cross-linked copolymer, short rheology, used in gels, cleaning and cleaning products, high electrolyte products, creams, lotions. Carbopol Ultrez 20: acrylate/C10-30 alkyl acrylate cross-linked copolymer, long rheology, shampoo, shower gel, cream/lotion, skin care and hair care gel with electrolyte. Pemulen TR-1: acrylate/C10-30 alkyl acrylate cross-linked copolymer, thickening emulsifier, short rheology, used in creams, lotions. Pemulen TR-2: acrylate/C10-30 alkyl acrylate cross-linked copolymer, thickening emulsifier, long rheology, used in emulsion.  

TAPS, the full name N-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid, CAS No. 29915-38-6; molecular weight 243.28; molecular formula: C7H17NO6S; it is white crystalline powder, soluble in water and other organic solvents , pH buffer range 7.7-9.1. Zwitterionic buffers for biochemical research and molecular biology are currently being used more and more widely in biochemistry, chemistry and medicine. It can be used as bio-buffering agents in biochemical diagnostic kits and DNA / RNA extraction kits. And high-end chemical reagents such as PCR diagnostic kits, cell culture must maintain strict pH values, and as a chelating agent for certain metal ions such as erbium, neodymium, etc., suitable for capillary electrophoresis analysis of DNA and DNA dye complexes , Can inhibit the activity of connexin pipeline, use TAPS physiological buffer in various carbonic anhydrase activities, pKa = 8.4.   Molecular Structure:   There are few reports on the synthesis process of TAPS. In 2010, Chen Gui'e and others studied the synthesis method of TAPS in detail. They use trishydroxymethylaminomethane (Tris) and 1,3-propane sultone (1,3-PS) as raw materials to make them react in alcoholic solvents. Specific operation: Weigh equimolar amount of Tris And 1,3-PS, and take an appropriate amount of alcohol solvent into a 250ml three-necked flask with condensing and refluxing, heating with stirring and refluxing; after a period of reaction, stop the reaction; at room temperature, after the reaction After the solution was allowed to stand and cooled to room temperature, it was suction filtered under reduced pressure. The TAPS crude filter cake was washed 2 to 3 times with an appropriate amount of absolute ethanol. The TAPS filter cake was taken out and placed in a vacuum drying oven to dry. stand-by.   In the above synthesis process, the reaction time, different alcohol solvents and the recycling of the reaction solvent have a certain effect on the TAPS yield and purity. The experimental results show that the reaction time is controlled at 6 hours, and the yield is the highest, reaching 70.3%. Continue to extend the reaction time, the yield decreases slightly; for the ethanol, n-propanol, isopropanol and n-butanol reaction solvents investigated, n-butanol The yield and purity of TAPS as the reaction solvent are the highest, 70.3% and 94.36%, respectively; in the reaction process, the recycling of the reaction solvent is beneficial to improve the yield and purity of TAPS.   Desheng has successfully carried out synthesis experiments based on the results of previous research and development. Now it has accumulated many years of production experience. The production of TAPS standards is high. Now the specifications of Desheng TAPS products are shown as follows: Appearance White crystalline powder Content (Ti) ≥99.0% Water insoluble matter ≤0.01% Loss on drying ≤1.0% pH(1% water solution) 4.4-6.0 Absorbance(260nm 0.1Mwater solution) ≤0.04 Absorbance(280nm 0.1Mwater solution) ≤0.02     The Warning and prevention: ◇ When impurities, foreign matters, abnormal odors, colors, severe moisture absorption and the expiration date are exceeded, the use is prohibited. ◇ Do not continue to use when turbidity appears. ◇ This product is a chemical reagent with strong corrosive properties, fasting, and direct touching by hand are prohibited. ◇ When spilled on the skin, rinse with plenty of water. ◇ If it splashes into the eyes, it can be immediately rinsed with a large amount of water. In an emergency, it should be sent to the hospital for treatment immediately.  

TAPS buffer is widely used, both as a buffer system commonly used in DNA screening systems and as a buffer component for RNA samples; It is used for electron transfer and phosphorylation studies of chloroplast thin layer preparation; During the drying process, it can protect the oxidation of oxyhemoglobin to methemoglobin; it can also be used as a background electrolyte for capillary zone electrophoresis for protein microanalysis. Desheng is skilled in technology. TAPS buffer is prepared from high-purity, high-quality TAPS. After strict filtration and sterilization, quality testing can be directly added to the medium. It should be noted that TAPS buffer has a pH range of 7.7-9.1 and a pKa (ion concentration set) of 8.4. The method of using TAPS buffer is to add it directly to the sterile medium or add the instrument to filter and sterilize when configuring the medium.   TAPS buffer can be used to control the solid combination of gas or liquid fugacity at high temperature and pressure, or at normal temperature and pressure. It can be solid or liquid. Buffers in chemical engineering are often called acid-base stabilizers, and are generally salts, such as strong acid weak bases or weak acid strong base salts. During the reaction or storage, the acid or base in the salt is gradually released to maintain a stable acid base value. The fHZ is provided externally. Pure solid silver and pure liquid silver chloride are balanced at a certain temperature and pressure to produce a fixed one. fHCl, used in H-O-Cl system. The buffering agent can suppress the change of the pH value of the plating solution during electroplating. For example, if boric acid is added to the nickel plating solution, its buffer range is close to the pH value of the plating solution of 5-6, so it can effectively suppress the pH value of the solution near the cathode caused by the plating process. In addition, in some other electroplating solutions, ammonium salts, acetates, citrates, etc. are also used.   In order to prepare a buffer solution of a certain pH, first select a weak acid whose pKaφ is as close as possible to the pH value of the buffer solution to be prepared, and then calculate the concentration ratio of the acid to the base, according to this concentration ratio, the required buffer solution can be prepared . The above mainly takes the buffer solution composed of weak acid and its salt as an example to explain its working principle, pH calculation and preparation method. The same method can be used for buffer solutions composed of weak bases and their salts.   The buffer solution is widely used in material separation and component analysis. For example, when identifying Mg2 ions, the following reaction can be used: The precipitate of white magnesium ammonium phosphate is soluble in acid, so the reaction needs to be carried out in an alkaline solution, but the alkalinity is too strong. A white Mg (OH)2 precipitate is formed, so the pH of the reaction needs to be controlled within a certain range, so the above reaction is carried out using a buffer solution composed of NH3 · H2O and NH4Cl while maintaining the pH of the solution.

Bicine buffer, dihydroxyethylglycine, CAS No. 150-25-4, is a biological buffer with a wide range of uses. Like Tris, it has superior buffering performance and is gradually replaced in some demanding biochemical tests Traditional phosphate buffer.   Bicine is an amino acid derivative with weak acidity, pH buffer range of 7.6-9.0, soluble in water and commonly used organic solvents such as DMF, DMSO, DMAc, etc. When the powdered bicine is formulated into a solution, sodium hydroxide is usually added to adjust the pH value, so as to meet the pH environment requirements required for experiment or detection. If the experimental environment needs to eliminate the interference of sodium ions, potassium hydroxide can be used instead of sodium hydroxide, and then the pH can be measured by a pH meter to adjust the mixing ratio of the Bicine solution and the lye.   Similar to the working principle of other biological buffers, Bicine is amphoteric. When acid and alkali are added to the reaction system or the pH of the reaction environment changes as the reaction progresses, it can act as a buffer to maintain the reaction system. pH value, which is intuitively important for reactions involving enzymes or proteins and accounting in biochemical detection. The stability and functionality of most organic biomolecules are very sensitive to pH.   The molecular structure of Bicne is similar to glycine. The hydrogen on the amino nitrogen atom is replaced by two hydroxyethyl groups, which reduces the hydrolysis ability of the amino terminal and enhances its stability. At the same time, it has the properties of amino acids and aminoethanol, and can form a metal complex with a single anion Thing. The buffer system configured with Bicine and sodium hydroxide will not participate in the chemical reaction of the reaction system, nor interfere with the experimental reaction, and is resistant to chemical and enzymatic hydrolysis.   Desheng is a manufacturer specializing in biochemical testing, in vitro diagnostic raw material production and research and development. It has rich experience in blood pretreatment, blood pretreatment and biochemical kits. At present, the mature product series include blood collection tube additives, chromogenic reagents, chemiluminescence Reagents and biological buffers including more than ten Good's reagents such as Bcine.  

Choosing the right biological buffer for the experiment can be a difficult task. There are many options available (Hepes, Mops, Tris, Bis-Tris, etc.), which can easily cause confusion.   After understanding these challenges, Desheng has prepared a short list for your reference when choosing buffer salts for research:   1. Buffer range Each buffer reagent has a pH range with the strongest buffer capacity. This buffer capacity is usually determined by the pKa of the buffer reagent. You must choose a buffer with a pKa value close to the middle of the desired range (typically, it is recommended to use a buffer with a pKa value that is at least within one pH unit of the target pH). The applicable pH range of each buffer 2. pH changes during the experiment Considering in advance whether the pH value may increase or decrease during the experiment is critical to the selection of buffers. If you think the pH will increase during the experiment, you must choose a buffer with a pKa slightly higher than the optimal value at the beginning of the experiment. The opposite is also true: if you think the pH will decrease, choose a buffer with a lower pKa.   3. Buffer concentration The buffer concentration must be adjusted to have sufficient buffering capacity for the experimental system. In general, if the buffer concentration is too low, the pH of the solution cannot be stabilized. Conversely, if the concentration is too high, the buffer is likely to affect the experiment. Generally, it is recommended to use a concentration higher than 25mM.   For systems in which hydrogen proton exchange is not active, a concentration of 25-100mM is generally recommended. For systems where proton exchange may occur, it is recommended to use a buffer concentration 20times higher than the molar concentration of the protons exchanged.   When preparing a buffer solution, remember to adjust the solution concentration to the intended use concentration.   4. Temperature change You must set the pH of the buffer solution according to the temperature at which the experiment will be performed. The temperature directly affects the pKa of the buffer, and thus the pH and buffering capacity of the system. This situation may be critical in biological systems, where accurate hydrogen ion concentrations are required for the reaction system to operate at maximum efficiency.   The pKa of some buffers (such as PIPES) is less sensitive to temperature changes, but other options (such as TRIS, ACES, TAPS, TES, or Tricine) are more affected by these changes.   The figure below shows the approximate pKa change according to the temperature of Desheng Biobuffer:   Approximate pKa change of Desheng Biobuffer Temperature   Desheng is one of the largest manufacturers of biological buffers in the world. Our products are shipped daily to top research centers and biotechnology companies in Europe, America and Asia. Our products are independently developed and produced. We can provide products that meet the quality and packaging requirements of our customers. We provide small packages and bulk packages (from grams to tons). They can be delivered worldwide to assist transportation, and provide comprehensive and powerful after-sales service. Looking forward to your call for further consultation! Tel: +86-711-3702650. Website: www.vacutaineradditives.com.  

As we all know, the domestic IVD market has a growing demand for immunoassays, and various types of immunoassays are emerging. Chemiluminescence, as a method with more accurate detection results, has gradually replaced ELISA immunoassays. According to the method, chemiluminescence can be divided into: direct chemiluminescence, enzymatic chemiluminescence, and electrochemiluminescence. Luminol and acridinium ester are used in enzymatic chemiluminescence and direct chemiluminescence, respectively. Today we will discuss the different applications of luminol and acridinium ester in chemiluminescence.   I   Chemiluminescent enzyme immunoassay Chemiluminescent enzyme immunoassay (CLEIA) is an enzyme-labeled immunoassay that uses a chemiluminescent agent as a substrate for an enzyme reaction. After two-stage amplification of enzyme and luminescence, it has high sensitivity. Peroxidase is used as a labeling enzyme, luminol is used as a luminescent substrate, and a luminescence enhancer is added to improve sensitivity and luminescence stability. The labeling enzyme used can also be alkaline phosphatase, the luminescent substrate is dioxetane phosphate, and the solid phase carrier is magnetic particles. Luminol chemiluminescence   II  Direct chemiluminescence immunoassay Chemiluminescence immunoassay (CLIA) is a type of immunoassay that directly labels antigens or antibodies with chemiluminescence agents. Acridinium ester is an ideal luminescent substrate and can be oxidized by hydrogen peroxide in an alkaline environment to emit light. The chemiluminescent agent used as a label should meet the following conditions: 1. Can participate in chemiluminescence reactions. 2. Stable conjugate reagent after coupling with antigen or antibody. 3. After coupling, high quantum effect and reaction power are retained. 4. Should not change or rarely change the physical and chemical properties of the labeled substance, especially the immune activity. The advantage of this method is that it is not affected by excitation light scattering or point difference. The figure below schematically shows the mechanism of direct chemiluminescence of the sandwich method. The figure below schematically shows the mechanism of direct chemiluminescence of the sandwich method.   III  Overall, Luminol and acridine ester luminescent agents are commonly used luminescent agents. Luminol is used as an enzymatic chemiluminescent substrate together with horseradish peroxidase for indirect chemiluminescence with a maximum wavelength of 425nm. The characteristics of the reaction are: high sensitivity and good stability of the enzyme. However, the disadvantage is that the longer excitation time results in a relatively slow speed, which may obscure antibody reaction sites during the labeling process. Currently, Antu Biology and others are using this method. As a direct luminescent marker, acridine ester needs to be carried out under alkaline conditions containing H2O2. The maximum wavelength of light emission is 430nm. The reaction characteristics are: the luminescence system is simple and fast, and the luminescence is completed within 5s, and no catalyst is needed, but the acridinium ester is unstable in the buffer salt and easily hydrolyzed. The manufacturer using this system is Abbott.   Desheng Technology is a manufacturer specializing in raw materials for blood testing and in vitro diagnostic reagents, focusing on intensive cultivation in the field for 15 years. It can provide acridinium ester, luminol and its supporting reagents. The products have been verified by more than 100+ manufacturers, the quality is guaranteed, the difference between batches is small, and a full set of subsequent technical services are provided. Welcome to contatct us for further consultation. Tel: +86-711-3702650. Website: www.vacutaineradditives.com

For many people, blood detection are far away from us. Everyone only sees the relevant detection process on TV, but many people certainly do not know that there is a chemiluminescent reagent behind the success of the detection. It is luminous ammonia luminol. The murderer often uses various methods to clean up the scene after the murder. When the scene is processed until the blood can not be found by the naked eye, it is necessary to rely on the luminol reagent to investigate the scene.   In fact, in the early twentieth century, benzidine was a commonly used reagent for detecting blood stains, but because benzidine and its salts are both toxic and carcinogenic substances, their solids and vapors are easy to enter the body through the skin, so this The method is gradually deprecated, and it is now banned in many countries such as China and the United States.   Then there was a comer. In the 1830s, German crime scene experts unintentionally discovered that Lumino would produce fluorescence after contact with blood. Later, Western countries began to study the mechanism. Until now, Lu Mino reagent has become the most widely used star reagent in blood stain survey in various countries.   Even if the blood at the crime scene has been wiped or cleared, forensics can still use Luminol to find their location. In fact, the investigator sprayed the luminol and activator solution in the area to be investigated, and the iron in the blood immediately catalyzed the luminescent reaction of luminol, causing it to produce a blue light. The amount of catalyst required for this reaction is very small, so Luminol can detect traces of blood. The glow lasts about 30 seconds.   Lumino is widely used in criminal investigation, bioengineering, chemical tracing and other fields. It is precisely because of its magical effect that it is sought after by many criminal investigation enthusiasts. The professional manufacturer of chemiluminescent reagents includes not only luminol, but also isoluminol, acridinium ester and other products, welcome to contact us for further consultation~!  

  When it comes to chemiluminescence or acridinium esters, you may think that it is a term that is too professional, far away from your life, even in the university's biochemistry major, it is rarely mentioned.However, if I say immune response, you are probably no stranger. Chemiluminescence immunoassay (chemiluminescence immunoassay, CLIA) started in the early 1980s and rapidly developed in the 1990s, becoming a development after fluorescence immunoassay, radioimmunoassay and enzyme-linked immunoassay Emerging immunodetection technology. It uses the specific reaction of antigen and antibody for detection, and uses isotopes, enzymes, chemiluminescent substances to amplify and display the detection signal, and is often used to detect trace substances such as proteins and hormones. In connection with actual life, that is, you go to the hospital to check the thyroid hormones, the diagnostic methods that sex hormones will use.     Immunological diagnosis occupies a very important position in clinical diagnosis. Common immunological techniques include radioimmunity, enzyme-linked immunity, chemiluminescence, electrochemiluminescence, and nanomagnetic particle chemiluminescence. Chemiluminescence immunoassay technology mainly has the advantages of high sensitivity, strong specificity, good reagent stability and long validity, wide detection linear range, simple operation, stable and fast method, many test items (as shown below), and high degree of automation. It has become the mainstream method of immunoassay technology and is widely used in various fields of clinical testing. Acridinium ester labeled antibody detection IgG     The acridinium ester mentioned above is a type of chemical substance that can be used as a chemiluminescent marker. In alkaline H2O2 solution, when the molecule of the acridinium ester is attacked by hydrogen peroxide ions, the substituent on the acridinium ring can It forms unstable dioxyethane with C-9 and H2O2 (hydrogen peroxide) on the acridinium ring, which decomposes into CO2 and electronically excited N-methylacridone. There are a total of 7 different groups of acridinium ester products, 1. AE-NHS (traditional acridinium ester); 2. DMAE-NHS; 3. Me-DMAE-NHS; 4. NSP-DMAE-NHS; 5 . NSP-SA-NHS; 6. NSP-SA; 7. NSP-SA-ADH.     These acridinium esters produced by Desheng have the characteristics of high product purity and small batch-to-batch difference. They also provide other immune reagents for matching use, provide a full set of technical services and technical support, please contact us for inquiries! Website: www.vacutaineradditives.com, Phone: +86-711-3702650.  

Acridinium ester, a type of chemical substance that can be used as a chemiluminescent marker, with a yellow powder appearance. In alkaline H2O2 solution, the molecules of acridinium ester are affected by hydrogen peroxide ions. The ring C-9 and hydrogen peroxide form unstable dioxane, which decomposes into CO2 and electron-excited N-methylacridone.   Acridinium ester reaction process The acridinium ester chemiluminescence process reacts quickly and has a low background, and can emit light in the presence of sodium hydroxide and hydrogen peroxide. During the oxidation reaction, the conjugate is decomposed without affecting the luminescence of the free acridinium ester; in addition, the acridinium ester chemiluminescent reagent has good stability and is easy to store. The difference between acridinium ester and other mainstream chemiluminescence reagent Type Marking, reaction system Main advantages Main disadvantages     Chemiluminescence Enzymatic Peroxidase, luminol, oxidant, enhancer, etc. Simple measurement method (rate method), low cost, high sensitivity The working curve drifts with time and the low-end slope shifts nonlinearly Spiral adamantane epoxide, alkaline phosphatase Simple measurement method (rate method), high sensitivity Non-enzymatic Acridinium ester, hydrogen peroxide high sensitivity Short luminescence time, complicated measurement method (integral method), in-situ injection (In Situ Injector), high instrument cost and maintenance cost, and high reagent cost Electrochemiluminescence Pulsed electric field excitation of ruthenium (Ru) complex High sensitivity and stable reagents Complex measurement methods (pulse excitation, intermittent measurement, flow cell), high instrument cost and maintenance costs, similar elements in the environment and samples can lead to background interference   Desheng Technology develops and produces 6 acridinium ester products with different groups. Although the substituents are different, they have the same acridinium ring. Whether they are acridinium ester or acridinium hydrazide, their luminescence principles are the same. The acridinium ester developed and produced by Desheng Technology belongs to laboratory production, and the quality control is stricter. Desheng has a professional technical R&D team, an excellent logistics partner, professional packaging services, and more than ten years of production R & D experience. It has the ability to provide end users with a strong problem-solving ability. Looking forward to you further consultation. Please contact tel:0711-3702650 for details.