China Wuhan Desheng Biochemical Technology Co., Ltd Company News

TAPS buffer is widely used, both as a buffer system commonly used in DNA screening systems and as a buffer component for RNA samples; It is used for electron transfer and phosphorylation studies of chloroplast thin layer preparation; During the drying process, it can protect the oxidation of oxyhemoglobin to methemoglobin; it can also be used as a background electrolyte for capillary zone electrophoresis for protein microanalysis. Desheng is skilled in technology. TAPS buffer is prepared from high-purity, high-quality TAPS. After strict filtration and sterilization, quality testing can be directly added to the medium. It should be noted that TAPS buffer has a pH range of 7.7-9.1 and a pKa (ion concentration set) of 8.4. The method of using TAPS buffer is to add it directly to the sterile medium or add the instrument to filter and sterilize when configuring the medium.   TAPS buffer can be used to control the solid combination of gas or liquid fugacity at high temperature and pressure, or at normal temperature and pressure. It can be solid or liquid. Buffers in chemical engineering are often called acid-base stabilizers, and are generally salts, such as strong acid weak bases or weak acid strong base salts. During the reaction or storage, the acid or base in the salt is gradually released to maintain a stable acid base value. The fHZ is provided externally. Pure solid silver and pure liquid silver chloride are balanced at a certain temperature and pressure to produce a fixed one. fHCl, used in H-O-Cl system. The buffering agent can suppress the change of the pH value of the plating solution during electroplating. For example, if boric acid is added to the nickel plating solution, its buffer range is close to the pH value of the plating solution of 5-6, so it can effectively suppress the pH value of the solution near the cathode caused by the plating process. In addition, in some other electroplating solutions, ammonium salts, acetates, citrates, etc. are also used.   In order to prepare a buffer solution of a certain pH, first select a weak acid whose pKaφ is as close as possible to the pH value of the buffer solution to be prepared, and then calculate the concentration ratio of the acid to the base, according to this concentration ratio, the required buffer solution can be prepared . The above mainly takes the buffer solution composed of weak acid and its salt as an example to explain its working principle, pH calculation and preparation method. The same method can be used for buffer solutions composed of weak bases and their salts.   The buffer solution is widely used in material separation and component analysis. For example, when identifying Mg2 ions, the following reaction can be used: The precipitate of white magnesium ammonium phosphate is soluble in acid, so the reaction needs to be carried out in an alkaline solution, but the alkalinity is too strong. A white Mg (OH)2 precipitate is formed, so the pH of the reaction needs to be controlled within a certain range, so the above reaction is carried out using a buffer solution composed of NH3 · H2O and NH4Cl while maintaining the pH of the solution.

Bicine buffer, dihydroxyethylglycine, CAS No. 150-25-4, is a biological buffer with a wide range of uses. Like Tris, it has superior buffering performance and is gradually replaced in some demanding biochemical tests Traditional phosphate buffer.   Bicine is an amino acid derivative with weak acidity, pH buffer range of 7.6-9.0, soluble in water and commonly used organic solvents such as DMF, DMSO, DMAc, etc. When the powdered bicine is formulated into a solution, sodium hydroxide is usually added to adjust the pH value, so as to meet the pH environment requirements required for experiment or detection. If the experimental environment needs to eliminate the interference of sodium ions, potassium hydroxide can be used instead of sodium hydroxide, and then the pH can be measured by a pH meter to adjust the mixing ratio of the Bicine solution and the lye.   Similar to the working principle of other biological buffers, Bicine is amphoteric. When acid and alkali are added to the reaction system or the pH of the reaction environment changes as the reaction progresses, it can act as a buffer to maintain the reaction system. pH value, which is intuitively important for reactions involving enzymes or proteins and accounting in biochemical detection. The stability and functionality of most organic biomolecules are very sensitive to pH.   The molecular structure of Bicne is similar to glycine. The hydrogen on the amino nitrogen atom is replaced by two hydroxyethyl groups, which reduces the hydrolysis ability of the amino terminal and enhances its stability. At the same time, it has the properties of amino acids and aminoethanol, and can form a metal complex with a single anion Thing. The buffer system configured with Bicine and sodium hydroxide will not participate in the chemical reaction of the reaction system, nor interfere with the experimental reaction, and is resistant to chemical and enzymatic hydrolysis.   Desheng is a manufacturer specializing in biochemical testing, in vitro diagnostic raw material production and research and development. It has rich experience in blood pretreatment, blood pretreatment and biochemical kits. At present, the mature product series include blood collection tube additives, chromogenic reagents, chemiluminescence Reagents and biological buffers including more than ten Good's reagents such as Bcine.  

Choosing the right biological buffer for the experiment can be a difficult task. There are many options available (Hepes, Mops, Tris, Bis-Tris, etc.), which can easily cause confusion.   After understanding these challenges, Desheng has prepared a short list for your reference when choosing buffer salts for research:   1. Buffer range Each buffer reagent has a pH range with the strongest buffer capacity. This buffer capacity is usually determined by the pKa of the buffer reagent. You must choose a buffer with a pKa value close to the middle of the desired range (typically, it is recommended to use a buffer with a pKa value that is at least within one pH unit of the target pH). The applicable pH range of each buffer 2. pH changes during the experiment Considering in advance whether the pH value may increase or decrease during the experiment is critical to the selection of buffers. If you think the pH will increase during the experiment, you must choose a buffer with a pKa slightly higher than the optimal value at the beginning of the experiment. The opposite is also true: if you think the pH will decrease, choose a buffer with a lower pKa.   3. Buffer concentration The buffer concentration must be adjusted to have sufficient buffering capacity for the experimental system. In general, if the buffer concentration is too low, the pH of the solution cannot be stabilized. Conversely, if the concentration is too high, the buffer is likely to affect the experiment. Generally, it is recommended to use a concentration higher than 25mM.   For systems in which hydrogen proton exchange is not active, a concentration of 25-100mM is generally recommended. For systems where proton exchange may occur, it is recommended to use a buffer concentration 20times higher than the molar concentration of the protons exchanged.   When preparing a buffer solution, remember to adjust the solution concentration to the intended use concentration.   4. Temperature change You must set the pH of the buffer solution according to the temperature at which the experiment will be performed. The temperature directly affects the pKa of the buffer, and thus the pH and buffering capacity of the system. This situation may be critical in biological systems, where accurate hydrogen ion concentrations are required for the reaction system to operate at maximum efficiency.   The pKa of some buffers (such as PIPES) is less sensitive to temperature changes, but other options (such as TRIS, ACES, TAPS, TES, or Tricine) are more affected by these changes.   The figure below shows the approximate pKa change according to the temperature of Desheng Biobuffer:   Approximate pKa change of Desheng Biobuffer Temperature   Desheng is one of the largest manufacturers of biological buffers in the world. Our products are shipped daily to top research centers and biotechnology companies in Europe, America and Asia. Our products are independently developed and produced. We can provide products that meet the quality and packaging requirements of our customers. We provide small packages and bulk packages (from grams to tons). They can be delivered worldwide to assist transportation, and provide comprehensive and powerful after-sales service. Looking forward to your call for further consultation! Tel: +86-711-3702650. Website: www.vacutaineradditives.com.  

As we all know, the domestic IVD market has a growing demand for immunoassays, and various types of immunoassays are emerging. Chemiluminescence, as a method with more accurate detection results, has gradually replaced ELISA immunoassays. According to the method, chemiluminescence can be divided into: direct chemiluminescence, enzymatic chemiluminescence, and electrochemiluminescence. Luminol and acridinium ester are used in enzymatic chemiluminescence and direct chemiluminescence, respectively. Today we will discuss the different applications of luminol and acridinium ester in chemiluminescence.   I   Chemiluminescent enzyme immunoassay Chemiluminescent enzyme immunoassay (CLEIA) is an enzyme-labeled immunoassay that uses a chemiluminescent agent as a substrate for an enzyme reaction. After two-stage amplification of enzyme and luminescence, it has high sensitivity. Peroxidase is used as a labeling enzyme, luminol is used as a luminescent substrate, and a luminescence enhancer is added to improve sensitivity and luminescence stability. The labeling enzyme used can also be alkaline phosphatase, the luminescent substrate is dioxetane phosphate, and the solid phase carrier is magnetic particles. Luminol chemiluminescence   II  Direct chemiluminescence immunoassay Chemiluminescence immunoassay (CLIA) is a type of immunoassay that directly labels antigens or antibodies with chemiluminescence agents. Acridinium ester is an ideal luminescent substrate and can be oxidized by hydrogen peroxide in an alkaline environment to emit light. The chemiluminescent agent used as a label should meet the following conditions: 1. Can participate in chemiluminescence reactions. 2. Stable conjugate reagent after coupling with antigen or antibody. 3. After coupling, high quantum effect and reaction power are retained. 4. Should not change or rarely change the physical and chemical properties of the labeled substance, especially the immune activity. The advantage of this method is that it is not affected by excitation light scattering or point difference. The figure below schematically shows the mechanism of direct chemiluminescence of the sandwich method. The figure below schematically shows the mechanism of direct chemiluminescence of the sandwich method.   III  Overall, Luminol and acridine ester luminescent agents are commonly used luminescent agents. Luminol is used as an enzymatic chemiluminescent substrate together with horseradish peroxidase for indirect chemiluminescence with a maximum wavelength of 425nm. The characteristics of the reaction are: high sensitivity and good stability of the enzyme. However, the disadvantage is that the longer excitation time results in a relatively slow speed, which may obscure antibody reaction sites during the labeling process. Currently, Antu Biology and others are using this method. As a direct luminescent marker, acridine ester needs to be carried out under alkaline conditions containing H2O2. The maximum wavelength of light emission is 430nm. The reaction characteristics are: the luminescence system is simple and fast, and the luminescence is completed within 5s, and no catalyst is needed, but the acridinium ester is unstable in the buffer salt and easily hydrolyzed. The manufacturer using this system is Abbott.   Desheng Technology is a manufacturer specializing in raw materials for blood testing and in vitro diagnostic reagents, focusing on intensive cultivation in the field for 15 years. It can provide acridinium ester, luminol and its supporting reagents. The products have been verified by more than 100+ manufacturers, the quality is guaranteed, the difference between batches is small, and a full set of subsequent technical services are provided. Welcome to contatct us for further consultation. Tel: +86-711-3702650. Website: www.vacutaineradditives.com

For many people, blood detection are far away from us. Everyone only sees the relevant detection process on TV, but many people certainly do not know that there is a chemiluminescent reagent behind the success of the detection. It is luminous ammonia luminol. The murderer often uses various methods to clean up the scene after the murder. When the scene is processed until the blood can not be found by the naked eye, it is necessary to rely on the luminol reagent to investigate the scene.   In fact, in the early twentieth century, benzidine was a commonly used reagent for detecting blood stains, but because benzidine and its salts are both toxic and carcinogenic substances, their solids and vapors are easy to enter the body through the skin, so this The method is gradually deprecated, and it is now banned in many countries such as China and the United States.   Then there was a comer. In the 1830s, German crime scene experts unintentionally discovered that Lumino would produce fluorescence after contact with blood. Later, Western countries began to study the mechanism. Until now, Lu Mino reagent has become the most widely used star reagent in blood stain survey in various countries.   Even if the blood at the crime scene has been wiped or cleared, forensics can still use Luminol to find their location. In fact, the investigator sprayed the luminol and activator solution in the area to be investigated, and the iron in the blood immediately catalyzed the luminescent reaction of luminol, causing it to produce a blue light. The amount of catalyst required for this reaction is very small, so Luminol can detect traces of blood. The glow lasts about 30 seconds.   Lumino is widely used in criminal investigation, bioengineering, chemical tracing and other fields. It is precisely because of its magical effect that it is sought after by many criminal investigation enthusiasts. The professional manufacturer of chemiluminescent reagents includes not only luminol, but also isoluminol, acridinium ester and other products, welcome to contact us for further consultation~!  

  When it comes to chemiluminescence or acridinium esters, you may think that it is a term that is too professional, far away from your life, even in the university's biochemistry major, it is rarely mentioned.However, if I say immune response, you are probably no stranger. Chemiluminescence immunoassay (chemiluminescence immunoassay, CLIA) started in the early 1980s and rapidly developed in the 1990s, becoming a development after fluorescence immunoassay, radioimmunoassay and enzyme-linked immunoassay Emerging immunodetection technology. It uses the specific reaction of antigen and antibody for detection, and uses isotopes, enzymes, chemiluminescent substances to amplify and display the detection signal, and is often used to detect trace substances such as proteins and hormones. In connection with actual life, that is, you go to the hospital to check the thyroid hormones, the diagnostic methods that sex hormones will use.     Immunological diagnosis occupies a very important position in clinical diagnosis. Common immunological techniques include radioimmunity, enzyme-linked immunity, chemiluminescence, electrochemiluminescence, and nanomagnetic particle chemiluminescence. Chemiluminescence immunoassay technology mainly has the advantages of high sensitivity, strong specificity, good reagent stability and long validity, wide detection linear range, simple operation, stable and fast method, many test items (as shown below), and high degree of automation. It has become the mainstream method of immunoassay technology and is widely used in various fields of clinical testing. Acridinium ester labeled antibody detection IgG     The acridinium ester mentioned above is a type of chemical substance that can be used as a chemiluminescent marker. In alkaline H2O2 solution, when the molecule of the acridinium ester is attacked by hydrogen peroxide ions, the substituent on the acridinium ring can It forms unstable dioxyethane with C-9 and H2O2 (hydrogen peroxide) on the acridinium ring, which decomposes into CO2 and electronically excited N-methylacridone. There are a total of 7 different groups of acridinium ester products, 1. AE-NHS (traditional acridinium ester); 2. DMAE-NHS; 3. Me-DMAE-NHS; 4. NSP-DMAE-NHS; 5 . NSP-SA-NHS; 6. NSP-SA; 7. NSP-SA-ADH.     These acridinium esters produced by Desheng have the characteristics of high product purity and small batch-to-batch difference. They also provide other immune reagents for matching use, provide a full set of technical services and technical support, please contact us for inquiries! Website: www.vacutaineradditives.com, Phone: +86-711-3702650.  

Acridinium ester, a type of chemical substance that can be used as a chemiluminescent marker, with a yellow powder appearance. In alkaline H2O2 solution, the molecules of acridinium ester are affected by hydrogen peroxide ions. The ring C-9 and hydrogen peroxide form unstable dioxane, which decomposes into CO2 and electron-excited N-methylacridone.   Acridinium ester reaction process The acridinium ester chemiluminescence process reacts quickly and has a low background, and can emit light in the presence of sodium hydroxide and hydrogen peroxide. During the oxidation reaction, the conjugate is decomposed without affecting the luminescence of the free acridinium ester; in addition, the acridinium ester chemiluminescent reagent has good stability and is easy to store. The difference between acridinium ester and other mainstream chemiluminescence reagent Type Marking, reaction system Main advantages Main disadvantages     Chemiluminescence Enzymatic Peroxidase, luminol, oxidant, enhancer, etc. Simple measurement method (rate method), low cost, high sensitivity The working curve drifts with time and the low-end slope shifts nonlinearly Spiral adamantane epoxide, alkaline phosphatase Simple measurement method (rate method), high sensitivity Non-enzymatic Acridinium ester, hydrogen peroxide high sensitivity Short luminescence time, complicated measurement method (integral method), in-situ injection (In Situ Injector), high instrument cost and maintenance cost, and high reagent cost Electrochemiluminescence Pulsed electric field excitation of ruthenium (Ru) complex High sensitivity and stable reagents Complex measurement methods (pulse excitation, intermittent measurement, flow cell), high instrument cost and maintenance costs, similar elements in the environment and samples can lead to background interference   Desheng Technology develops and produces 6 acridinium ester products with different groups. Although the substituents are different, they have the same acridinium ring. Whether they are acridinium ester or acridinium hydrazide, their luminescence principles are the same. The acridinium ester developed and produced by Desheng Technology belongs to laboratory production, and the quality control is stricter. Desheng has a professional technical R&D team, an excellent logistics partner, professional packaging services, and more than ten years of production R & D experience. It has the ability to provide end users with a strong problem-solving ability. Looking forward to you further consultation. Please contact tel:0711-3702650 for details.  

    The chromogenic substrate TOPS, the full name of N-ethyl-N- (3-sulfopropyl) -3-methylaniline sodium salt, is similar to TOOS, the difference in molecular structure is that the sulfopropyl group on the single atom connected to the benzene ring With a hydroxyl group, the stability is slightly stronger, and all have the characteristics of high water solubility and high detection sensitivity. Like TOOS, TOPS can also be used for uric acid detection in kidney function kits. The chromogen used by uric acid detection kits of different companies is slightly different (the chromogenic substrate ESPMT used by some companies refers to TOPS). In addition, TOPS is also commonly used for liver function series free fatty acid detection and renal function series creatinine detection. The following is an example of the principle of uric acid detection.        During clinical testing, first collect venous blood from the test subject. The subject should be fasting for 12 hours. It is best not to drink alcohol in the morning and 12 hours. The blood collection tube should be disinfected. The blood sample is not hemolytic. The detection of uric acid is to first catalyze the complete oxidation of uric acid with oxygen and water to allantoin, carbon dioxide and hydrogen peroxide H2O2. H2O2 reacts with TOPS and 4-AAP under the catalysis of POD. TOPS is oxidized into a chromogenic product, and the absorbance is measured with a spectrophotometer. The intensity of the absorbance is proportional to the uric acid content, so that uric acid analysis is performed. This is a typical biochemical detection method using two-step enzymatic reaction to measure.         Desheng Technology has more than ten years of experience in R&D, production and sales in the field of diagnostic raw materials, especially in the development and improvement of new Trinder's reagents in the aniline sodium salt derivative series. It performs functional groups on the basis of traditional chromogenic substrates. Replacement of the increase and decrease, continue to make optimization and performance improvement for the needs of clinical testing!

     The full name of TOPS (CAS No.:40567-80-4) is N-ethyl-N-(3-sulfopropyl) -3-methylaniline sodium salt, which is a white to light brown powder, also a member of the new Trinder's reagent family. Maybe you think the name sounds humongous, and it is a vocabulary that a fully professional pharmacist only understands. It is even more difficult to relate to your life. If you think so, you are wrong. You may not have heard of this name, but I think most of you or people around you have done biochemical tests in the hospital, that is, liver function, kidney function, uric acid, cholesterol and other tests. Most of these tests are carried out by automatic biochemical analyzers, uric acid analyzers, etc. in conjunction with the corresponding kits. And one of the core raw materials in these kits is the TOPS we talk about today.        In humans and primates, uric acid is the final product of purine metabolism. It is produced by the oxidation of xanthine and hypoxanthine by xanthine oxidase and is excreted in the urine. High serum uric acid and hyperuricemia are associated with insulin resistance, cardiovascular disease and gout. The mechanism leading to hyperuricemia is usually increased uric acid production or decreased urine excretion. Increased serum uric acid may be a sign of kidney disease, so the early diagnosis of kidney disease can be indirectly through the test of uric acid.   TOPS Product description Chinses name N-ethyl-N- (3-sulfopropyl) -3-methylaniline sodium salt English name Sodium 3-(N-ethyl-3-methylanilino)propanesulfonate CAS No. 40567-80-4 Hs code 2922199090 Molecular formula C12H18NNaO3S, H2O Molecular weight 297.34 Appearance White or light brown powder Storage condition 0-5℃，away from light and moisture Transport condition Room temperature（20-25℃） Maximum absorption wavelength 550 nm Oxidation reaction Applacation This product is used for the colorimetric determination of uric acid and cholesterol. It has the characteristics of good water solubility, high sensitivity and strong stability. Cholesterol colorimetric determination; water-soluble reagent, used for catalase photometric determination.         In the presence of hydrogen peroxide and peroxidase, TOPS reagent is formed during the oxidative coupling reaction with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazolone hydrazone (MBTH) Very stable purple or blue dye. The substrate is enzymatically oxidized by its oxidase to produce hydrogen peroxide. The concentration of hydrogen peroxide corresponds to the substrate concentration. Therefore, the amount of the substrate can be determined by the color development of the oxidative coupling reaction to obtain a relatively accurate test result of the concentration of the analyte. TOPS produced by Desheng has obtained the quality certification of more than 100 manufacturers nationwide. Because of its color rendering sensitivity, color stability and anti-interference, as well as the accuracy and precision of specific measurement items, it has been well received by customers. You are welcome to inquire, the company will provide you with quality products and excellent services, so that your products rank among the industry's leading level! Company website: https://www.vacutaineradditives.com, Phone No.: +86-711-3702650.

The full name of the chromogenic substrate MAOS is N-ethyl-N- (2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt monohydrate, CAS No. is 82692-97-5. As other new Trinder ’s reagents, it has high water solubility and sensitivity much higher than traditional chromogenic reagents commonly used in some laboratories, so it is widely used in clinical testing and biochemical reagent kits.   First, the biggest feature of MAOS is that the maximum absorption wavelength of its oxidized product is far more than that of ordinary chromogenic reagents. Even in the new Trinder ’s reagent, its product UV absorption wavelength is 630nm, which is quite high. The maximum absorption wavelength of products of many chromogenic reagents is in the visible light region. If testing human blood or other body fluids, due to the complex components in the sample to be tested, the sample itself contains a small amount of components that absorb wavelengths in the visible light region or absorb with chromogenic products. The wavelengths are close, which will make the detection result high. If you use something like MAOS, the maximum absorption wavelength of the product is in the ultraviolet region and it is relatively high, which will greatly reduce interference. Therefore, it is recommended to use MAOS as a chromogenic substrate for some biochemical testing items that require highly accurate values.   Secondly, the chromogenic reaction of MAOS chromogenic substrate has a wider adaptability to the pH of the reaction system, which greatly improves its adaptability. The reaction of some chromogenic reagents is often performed under acidic conditions, but the biochemical detection usually requires the participation of enzymes. We know that the catalytic effect of enzymes is relatively sensitive to the pH of the reaction environment, and it may not match the chromogenic reagent Inactivation will be greatly restricted in use, and MAOS reagents do not have this problem.   Another point is that the MAOS chromogenic reaction has a larger molar absorption intensity, so its sensitivity is relatively high. It should be noted that MAOS will fade when the detection time is too long, so the MAOS detection needs to be completed in time and cannot be interrupted. In addition, MAOS is also relatively safe. Unlike DAB and other biphenyl chromogenic reagents, although the price is relatively low, it has certain carcinogenicity or mutagenicity.   Desheng Technology has been committed to the development, production and sales of various chromogenic substrates, especially TOOS, MAOS, ADPS, etc. have a good reputation at home and abroad, and have good cooperation with many biochemical kit manufacturers Relationship, and jointly contribute to the field of domestic diagnostic reagents!

As a new Trinder's reagent, MAOS occupies a pivotal position in it. It is a white powder that is easily soluble in water. Chinese name: N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3 , 5-Dimethylaniline sodium salt monohydrate, sensitive to light and humidity, CAS number: 82692-97-5, purity requirement> 99%, molecular structure is shown in the following figure:   Application of MAOS The new Trinder's reagent is a highly water-soluble aniline derivative, which is widely used in diagnostic tests and biochemical tests. It has several advantages over conventional color-generating reagents in the colorimetric determination of hydrogen peroxide activity. The new Trinder's reagent is stable enough to be used in both solutions and test line inspection systems. In the presence of hydrogen peroxide and peroxidase, the new Trinder's reagent during the oxidative coupling reaction with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazole sulfone hydrazone (MBTH), Forms a very stable purple or blue dye. The molar absorbance of the coupling dye with MBTH is 1.5-2 times higher than that with 4-AA; however, the 4-AA solution is more stable than the MBTH solution. The substrate is enzymatically oxidized by its oxidase to produce hydrogen peroxide. The concentration of hydrogen peroxide corresponds to the substrate concentration. Therefore, the amount of substrate can be determined by the color development of the oxidative coupling reaction. Glucose, alcohol, acyl-CoA and cholesterol can be used to detect those substrates coupled with the new Trinder's reagent and 4-AA. There are 10 new Trinder's reagents, and the use of MAOS members is also very important. For specific substrates, testing different types of new Trinder's reagents is necessary to develop the best detection system.   Product introduction Oxidizing chromogen reagents, high water solubility, stable aniline analogues, a wide pH range for color development and oxidation reactions   Preparation of detection solution-MAOS (other substrate configuration methods are basically the same) 1. Dissolve 20 mg MAOS in 10 ml PBS to prepare a 6.6 mM MAOS solution. (The specific solubility is adjusted as needed) 2. Dissolve 14 mg 4-aminoantipyrine (4-AA) in 10 ml PBS to prepare a 6.6 mM 4-AA solution. 3. Prepare 2 U / ml horseradish peroxidase solution in PBS. 4. Mix equal volumes of each solution to prepare a test solution. Store the test solution in the dark at 4 ℃   Detection steps 1. Prepare a sample solution for the enzymatic oxidation reaction. The pH value of the buffer solution should be 5.5-9.5. 2. Use the same buffer to prepare a standard solution containing a known amount of substrate. 3. Add appropriate units of oxidase to the sample solution, followed by an equal volume of detection solution. 4. Incubate the mixture at room temperature or 37 ° C for 30 minutes to 1 hour. 5. Determine the O.D. value at 555 nm. 6. Prepare a standard curve and determine the substrate concentration in the sample solution     MAOS belongs to one of Desheng Biochemical's key sales varieties. At the same time, TOOS, TOPS, ADOS, ADPS, ALPS, DAOS, HDAOS, MADB and other new Trinder's reagents are available for customers to choose. Desheng always upholds: Honesty first; quality first, technology leading; customer-oriented, symbiosis and win-win; pioneering and innovative, the pursuit of excellence in business philosophy, dedicated to providing our customers with quality products and services, welcome to consult and cooperate!  

TOOS is also called EHSPT (N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt), which is a highly water-soluble sodium salt of aniline. It is sensitive when performing Trinder reaction. It is much higher than traditional phenol, aniline, ADA and other color-developing substrates. Clinical biochemical testing is often used in liver function kits, blood glucose metabolism kits, etc. In the presence of hydrogen peroxide and peroxidase POD, Trinder's reagent forms a very stable orange or red quinone with 4-aminoantipyrine (4-AAP). The molar absorbance of TOOS and MBTH coupling dye is 1.5-2 times higher than that of 4-AA coupling dye; however, 4-AAP solution is more stable than MBTH solution, so Trinder ’s reagent is more used with 4-AAP. When tested with a biochemical kit, the measured indicators such as uric acid, glucose, glycated albumin, and 1,5-anhydroglucitol are equal to the enzymatic oxidation of its oxidase to produce hydrogen peroxide. The concentration of hydrogen peroxide corresponds to the concentration of the substance to be tested Therefore, the amount of the analyte index can be determined by the color development of the oxidative coupling reaction. Certainly, the indicators of enzyme activity can also be measured, such as the adenosine dehydrogenase detection kit and 5'-nucleotidase detection in the liver function series, which is the reaction of the enzyme to be detected and its corresponding catalytic substance to generate peroxide Hydrogen is then reacted with the generated hydrogen peroxide through the coloring substrate TOOS coupling 4-AAP, and the final coloring product production rate corresponds to the activity of the tested enzyme, thereby achieving the purpose of measuring the index. The TOOS color development substrate developed by Desheng Technology has the characteristics of high purity, high water solubility, and low interference ion. It is very convenient to use when it is used, and the company also produces Tris buffer used with it for biochemical detection. Wait, the quality and service are well received!