China Wuhan Desheng Biochemical Technology Co., Ltd Company News

In our biochemical experiments, such as Trinder's reaction of chromogen substrate, acridine ester of luminescent substrate and various enzyme reactions, we use biological buffers, such as bicine, Tris, mops, and the most basic acetate and phosphate buffer systems. It can be said that as long as there are pH requirements for the reaction environment, buffers are needed in the system. Buffer in delivery The reason why the buffer solution can adjust pH is that it is a weak electrolyte, which is partially dissociated in the solution and partially exists in the form of molecules, and does not show acidity and alkalinity. Its molecules form a dynamic equilibrium with their ionic conjugated acids or bases (pH = PKA + LG, dissociated concentration is higher than that of the non dissociated concentration). If the pH of the system changes, the sub dynamic equilibrium will move to reach a new equilibrium, thus adjusting the pH.   The effective buffer range of a buffer system is usually in the range of two pH units with pKa value as the midpoint, that is, the effective pH range of the buffer = pKa ± 1. Therefore, when the pH of the buffer solution is equal to the pKa of the buffer, the buffer capacity is the largest. If we want to design a new buffer system, we only need to find out and select the buffers whose pKa value is equal to the required pH value.   In biochemical experiments, there are many commonly used biological buffers. Phosphate is the most widely used and basic buffer in biochemical research. Phosphoric acid is a ternary acid. Because they are secondary dissociation and have two pKa values, the pH range of the buffer prepared with them is the widest, and potassium salt is better than sodium salt, because sodium salt is difficult to dissolve at low temperature and potassium salt is easy to dissolve, but if SDS is prepared- The buffer solution of polyacrylamide gel electrophoresis can only use sodium phosphate instead of potassium phosphate, because SDS (twelve alkyl sulfate) will produce insoluble twelve alkyl potassium sulfate with potassium salt.   Phosphate is easy to be prepared into various concentrations of buffer solution, suitable for a wide range of pH, pH is less affected by temperature, pH change after dilution is small, and pH change after ten times dilution is less than 0.1. However, in the solution system with metal ions, phosphoric acid system will associate with common Ca ions, Mg ions and heavy metal ions to form precipitation.   The Tris buffer produced by us has been used more and more in biochemical research, and has a tendency to exceed phosphate buffer. For example, Tris buffer has been used in SDS- polyacrylamide gel electrophoresis, and phosphate is rarely used. The ACD solution used in our PrP blood collection vessel needs sodium citrate and citric acid to adjust the pH. the citrate here not only regulates the osmotic pressure, but also acts as a buffer. It is also a ternary acid like phosphoric acid.

MOPS, or 3-morpholinopropanesulfonic acid, is a widely used and very important buffer. MOPS buffer itself belongs to Good's buffer, the buffer range is between 6.5~7.9, has good pH stability, is highly polar, is inert to a variety of chemical reagents and enzymes, does not participate in and does not interfere with the process of biochemical reactions, and is chemically resistant to enzymes. The reaction has no inhibitory effect, so it can be specially used for the research of organelles and extremely volatile, pH-sensitive proteins and enzymes.   The main application areas of MOPS   1. As a running buffer in electrophoresis and protein purification in chromatography;   2. Prepare a variety of agar media for the cultivation of bacteria, yeast and mammalian cells. However, higher than 20mM The concentration of is not recommended for mammalian cells;   3. It can be used as a lysis buffer for E. coli cells;   4. As the eluent in gel filtration chromatography;   5. Used in Northern hybridization, as a buffer for RNA isolation and membrane transfer;   6. Suitable for the determination of bicinchoninic acid (BCA);   7. Used in the study of electron transfer and phosphorylation of chloroplast thin layer preparation.     Precautions for using MOPS   1. MOPS biological buffer reagent is a zwitterionic buffer. If the dosage of MOPS is very small each time, it is generally used appropriately after appropriate dispensing.   2. Generally, the biological buffer can easily change the color of the liquid to yellow after encountering light, and the light yellow can be used.   If the color is too dark, it should not be used again.   3. The use of MOPS biological buffer reagent requires special attention. Production safety and personnel health must not be sloppy. Therefore, laboratory clothes should be worn well and disposable gloves should be worn for operation. Once the solution splashes into the eyes, or touches slightly, you must use a lot of water to rinse immediately, and go to the hospital immediately.     MOPS production method   The mainstream production method of MOPS is completed in the following six steps, which can save costs, reduce pollution, and avoid safety risks. The preparation method of this MOPS uses water as a solvent and no organic solvents are used in the process. While reducing production costs, it reduces the risk of health hazards to operators, and there is no trouble of recycling and processing organic solvents, and there is no problem of environmental pollution. The main steps are morpholine dissolution, reaction to generate 3-morpholinepropanesulfonic acid aqueous solution, dehydration to obtain crude 3-morpholinepropanesulfonic acid, crude product recrystallization, crystal suction filtration and concentration, cooling, crystallization, and 3-morpholinopropane The process of sulfonic acid.   Desheng is a diagnostic reagent raw material manufacturer with many years of R&D and production experience. The purity of MOPS products produced is ≥99%, the water solubility is good, the production process is stable, and it has a strict quality management system to ensure that it provides customers with high-quality products and services. The company's products have been sold to many countries around the world, please call for more details!

As a chemiluminescence reagent, luminol is widely used in blood detection. It was synthesized as early as 1853. Since Albrecht first reported the chemiluminescence reaction of luminol with oxidant in alkaline solution in 1928, it was found for the first time that this compound has a wonderful property, which can emit blue light when it is oxidized.   A few years later, someone thought of using this property to detect blood stains. The blood contains hemoglobin, and the oxygen we breathe in from the air is transported to all parts of the body by this protein. Hemoglobin contains iron, which catalyzes the decomposition of hydrogen peroxide, turning hydrogen peroxide into water and monooxygen, which then oxidizes luminol to make it glow.     When examining bloodstains, luminol reacts with heme, a protein responsible for transporting oxygen in hemoglobin, showing a blue-green fluorescence. This detection method is extremely sensitive. It can detect only one millionth of blood. Even if a small drop of blood drops into a large tank of water, it can be detected. The luminol reaction in criminal investigation, generally speaking, is that at the scene of a murder, as long as blood splashes out and touches any object, No matter what kind of cleaning method is used afterwards, as long as the luminol reagent is sprayed on it and observed in the dark environment, there will be blue and white fluorescence due to fluorescence reaction in the place with blood stains.   The disadvantages of luminol: 1. Luminol fluoresces in the presence of copper, copper containing alloys, horseradish or some bleaching agents. So if the crime scene is completely bleached, luminol's fluorescence will strongly mask the presence of any blood stains. 2. Luminol can detect a small amount of blood in animal blood and urine, so if there is urine or animal blood in the room to be tested, the test results will be biased. 3. Luminol reacts with excreta and emits the same light as blood. 4. Luminol may interfere with other tests, but luminol does not interfere with DNA extraction. 5. Luminol needs to be used in a dark environment, otherwise the fluorescence is difficult to identify 6. Luminol's time to shine is limited, so we should seize the time to take photos   There are several ways to avoid interference in inspection   1. Let the site dry for a few days, the interference of bleach will disappear, and the bloodstain can make luminol glow even after many years.   2. Use a compound that can inhibit the interference of hypochlorite. It's obvious that antioxidants should not be used because they will inhibit the reaction of blood stains with luminol. According to the chemical structure of hypochlorous acid, such as the chlorine atom it contains, suitable inhibitors have been found. The safest way is to use luminol luminescence method to detect the suspected bloodstain, and then use other methods to determine that it is indeed bloodstain. Moreover, after being treated by luminol, the DNA contained in the bloodstain has not been destroyed, and it can be extracted for identification.   Desheng is an old chemical reagent company specializing in the development and production of blood test reagents. Luminol is one of the most commonly used liquid-phase chemiluminescence reagents because of its simple structure, easy synthesis, good water solubility and high luminescent quantum efficiency.

Luminol is also called luminol and its chemical name is 3-aminophthalic hydrazide. It is often used in chemiluminescence immunoassay biochemical systems. The appearance is yellow crystal or beige powder at room temperature, which is a relatively stable chemical reagent. It is often used for blood testing in criminal investigation scenes. When luminol reagent is sprayed on the blood, it will oxidize with active oxygen and release blue-violet fluorescence, which is called luminol reaction.     The chemiluminescence analysis method is widely used in the direct determination of drugs and biochemical samples because of its high sensitivity, wide linear range, and simple instrument operation. Studies on the determination of ketotifen fumarate by chemiluminescence and electrochemiluminescence analysis have also been reported. However, the study on the determination of ketotifen fumarate based on potassium permanganate chemiluminescence system has not been reported. After experimentation, it is found that in alkaline medium, potassium permanganate can oxidize luminol to produce a luminescence signal, and ketotifen fumarate has a strong sensitizing effect on the luminescence signal.   For this reason, we conducted experiments and analyzed the following conclusions:   1 Flow injection chemiluminescence signal system of flow injection chemiluminescence signal.   In an alkaline medium, potassium permanganate can oxidize luminol to produce chemiluminescence. When ketotifen fumarate is added to the reaction system, the chemiluminescence intensity is significantly enhanced.   2 Chemiluminescence spectrum   The maximum emission wavelength of the two luminescence systems is about 425 nm, which is consistent with the maximum emission wavelength of the luminol luminophore, indicating that the luminophores of the two chemiluminescence systems are excited state 3-aminophthalate. The luminescence intensity of the luminol-potassium permanganate-ketotifen fumarate system is significantly higher than that of the luminol-potassium permanganate system, indicating that the addition of ketotifen fumarate enhances the sensitivity of luminol-permanganate The luminous intensity of the potassium acid system.    3 Selection of flow path and flow   After experimentation, it is found that different mixing modes of the solution have a great influence on the luminescence signal. The various flow paths of the selected system were compared. The influence of different flow rates on the luminescence signal of the system was investigated. The experiment shows that with the increase of the flow rate, it first increases and then decreases, and reaches the maximum value when the flow rate reaches 1.9 mL·min. The test selects the flow rate of each pipeline to be 1.9 mL·min.   4 Selection of the concentration of sodium hydroxide solution   In 0.02～0.3 mol·L-4. Within the scope, the influence of the concentration of sodium hydroxide solution on the luminescence intensity of the reaction system was investigated. The results show that when the concentration of sodium hydroxide solution is 0.05～0.15Mol·L-1, the maximum and basically unchanged, the test chooses the concentration of sodium hydroxide solution to be 0.1 Mol·L-4.   5 The choice of oxidant and its concentration   The experiment investigated the influence of different oxidants such as potassium permanganate, potassium ferricyanide, hydrogen peroxide, potassium periodate on the chemiluminescence signal of the system. The results show that when potassium permanganate is used as the oxidant, the chemiluminescence sensitivity signal value is large and stable. Its concentration is 5.0×10-4mol·L-1. Therefore, the concentration of potassium permanganate solution is 5.0×10-4mol·L-1.   6 Selection of the concentration of luminol solution   Luminol is the main luminescent substance, and its concentration has a greater impact on the luminous intensity, detection sensitivity, and linear range. The experiment investigated the influence of luminol solution concentration in the range of 2.0×10-4～1.0×10-4mol·L_1 on the luminescence signal of the system. The results showed that as the concentration of luminol solution increased, chemiluminescence The increasing intensity indicates that the concentration of the luminol solution should not be too large; if the concentration of the luminol solution is too small, the luminescence signal value is small and the stability is poor. In consideration of stability and sensitivity, the concentration of luminol solution was selected as 3.0×10-4mol·L-1.   Hubei Xindesheng Materials specializes in the production and development of chemiluminescence reagents, luminescent substrates, biological buffers, blood collection tube additives and enzyme preparations. Desheng has been established for decades and has its own R&D team. It has been researching and developing chemiluminescence reagents for many years. There are many kinds of raw chemical products under its control. At present, the products produced by Desheng have been sold to many countries around the world, and they have been repurchased with praise, and have reached long-term cooperation with many enterprises.

HEPES is a kind of nonionic amphoteric buffer. The main component of HEPES buffer is 2 - [4 - (2-hydroxyethyl) - 1-piperazinyl] ethanesulfonic acid. HEPES is a kind of nonionic amphoteric buffer with good buffering capacity in the range of pH 7.2-7.4. In this condition, the lid of the cell culture bottle should be tightened to prevent a small amount of carbonate needed in the culture medium from scattering into the air.   Most of the culture media contain phenol red as pH indicator. The acidic culture media are orange yellow, and the alkaline culture media are dark red. HEPES is expensive and may be toxic to some cells at high concentrations, HEPES can be used as buffer instead of bicarbonate to remove the limitation of high concentration of CO2 culture environment. In practice, it is not so simple. Dissolved CO2 and bicarbonate are also very important for good cell growth.   There are two ways to use HEPES   (1) HEPES can be directly added into the prepared culture medium according to the required concentration, and then filtered to remove bacteria. Add 2.38g HEPES into every 1000ml culture medium, adjust pH to 7.2 with LN NaOH after dissolving, filter and use after sterilization. The concentration of HEPES was 10 mmol / L.   (2) Before use, 99 ml of culture medium was added to 1 ml of storage medium, and the final concentration was still 10 mmol / L. Methods: 23.8g HEPES was dissolved in 90ml double distilled water, adjusted to pH 7.5-8.0 with LN NaOH, and then diluted to 100ml with water, filtered and sterilized, sub packed into small bottles (2ml / bottle), stored at 4 ℃ or - 20 ℃.     4-hydroxyethyl piperazine ethanesulfonic acid has no toxic effect on cells. It is a hydrogen ion buffer, which can control the constant pH range for a long time. When the final concentration is 10-50 mmol / L and the culture medium contains 20 mmol / L HEPES, the buffer capacity can be achieved. It can be used as biological buffer, reaction buffer, pre hybridization buffer and hybridization buffer for separation and analysis of RNA nuclear components, and for RNA and t4rna.   Molecular biology grade is used for RNA3 '- end labeling with t4rna ligase chemicalbook, separation and analysis of reaction buffer components, pre hybridization buffer and buffer components of nuclear RNA. Cell to cell adhesion, short-term cell aggregation culture, tissue and cell cleaning buffer.   Desheng began to develop and produce vascular additives, color reagents, chemiluminescent reagents and biological buffers in 2005. It has a long history of research on biological buffers, has a mature technology and R & D team, and has excellent sales service and logistics service. In addition to HEPES, there are Tris, caps, taps, bicine and pipes products for biological buffers. If you need to call, Desheng welcomes your call.

DNA radiation damage usually occurs in two ways: direct and indirect. In direct action, DNA itself is ionized. The indirect effect involves the radiolysis of the solution around DNA and the subsequent interaction between DNA and radiolysis products.   It is estimated that 80% of the lethal effects of high LET radiation are caused by direct effects. Therefore, it is necessary to study the DNA damage induced by high LET radiation, which is also helpful to clarify the molecular mechanism of high relative biological effect of high LET radiation.   TRIS base powder   In the study of DNA damage caused by ionizing radiation, it is obviously helpful to obtain more accurate and reliable experimental results to select the appropriate DNA dissolution system. The most common dissolving system of DNA is te buffer, and its main components are 10 mmol / L tris (trimethyl aminomethane) and 1 mmol / L EDTA (ethylenediamine tetraacetic acid) at pH 8.0. Let's look at the main principles of the study.   PUC19 plasmid DNA dissolved in pure water, 10 mmol / L Tris base, 1 mmol / L led RA and te buffer was irradiated by 100 keV / p.m carbon ion beam (initial energy 290 MeV / U). The proportion of various DNA molecules in different solutions was analyzed by agarose gel electrophoresis, and the average number of single strand breaks (SSB) and double strand breaks (DSB) in each plasmid was calculated at different doses.   It was found that Tris could significantly protect plasmid DNA from carbon heavy ion irradiation by inhibiting the production of SSB and DSB, while EDTA could enhance the production of SSB and inhibit the formation of DSB.   Electrophoretogram of pUC19 DNA molecule in different solution and irradiation dose   It can be seen from the above figure that with the increase of irradiation dose, the percentage of supercoiled DNA in total DNA decreases. Compared with water and EDTA, the content of supercoiled DNA in Tris and te buffer decreased slowly. When the dose increased to 150Gy, the content of supercoiled DNA in Tris and te buffer was still more than 80%, but the content of supercoiled DNA in EDTA and water was less than 6O%. The results showed that the DNA in tri produced less strand breaks than that in water and EDTA.   A series of results show that Tris has obvious protective effect on DNA strand break damage induced by 100 keV / L ~ m carbon ion irradiation. In Tris and te buffer solutions, the supercoiled DNA existed all the time and showed obvious protection compared with pure water and EDTA solution.

Before we learn about biological buffers taps, let's know what is a diagnostic kit? What is the relationship between taps and kit?   Diagnostic kit is a kind of qualitative and quantitative test for body fluids, blood and other substances reflecting human health.   3-((1,3-Dihydroxy-2-(Hydroxymethyl)Propan-2-Yl)Amino)Propane-1-Sulfonic Acid (TAPS) is a kind of amphoteric buffer widely used in biochemistry and molecular biology. It has a pH buffer range of 7.7-9.1 and is soluble in water (25g / 50ml). In clinical diagnosis, taps as a biological buffer is used in biochemical diagnostic kit, DNA / RNA extraction kit and PCR diagnostic kit.   It can be seen that taps is one of the raw materials of our kit.   Desheng buffer taps   [purpose] as a buffer system commonly used in DNA screening system, it can also be used as a buffer component of RNA sample. It is suitable for electron transfer and phosphorylation research of chloroplast thin layer preparation. It can protect oxyhemoglobin from oxidation to methemoglobin during freeze-drying process. It can also be used as a background electrolyte for protein microanalysis by capillary zone electrophoresis.   [product advantage] purity ≥ 99%, good water solubility, stable process, can ensure the appearance of pure white crystal.   [note] the secondary product only needs to be stored at room temperature. It can't see strong light. It needs a container device with better shading. The place where it is placed should be moistureproof and dry. If the secondary product can't be used up at one time, it is recommended to pack it into the amount you need each time and seal the bottle well, so as to avoid moisture absorption or product quality problems.   Taps buffer, toos, Maos, tops and other buffer products produced by Desheng have white crystal, high purity (more than 99%), good solubility, and absorbance < 0.05, which are different from light blue or brown products on the market.   Desheng technology's product quality and technology in China's local market has been widely recognized and used by customers. We will continue to develop and research advanced biochemical reagents to meet the needs of the development of global medical laboratory science and technology and strive for human health.

Heparin is common in clinical blood test with sodium salt and lithium salt, which has unique application value. Heparin has low chelating force, little influence on the movement of water molecules, less interference on blood components, no influence on the volume of red blood cells, and no hemolysis. Therefore, heparin is recommended as an anticoagulant in a variety of tests based on whole blood or plasma.   Heparin lithium and heparin sodium have anticoagulant effects in clinic. They are mainly used in thromboembolic diseases, myocardial infarction, cardiovascular surgery, cardiac catheterization, extracorporeal circulation, hemodialysis and so on. Because of the different ions combined, their dissociation degree is different. The effect of heparin lithium is better than that of heparin sodium.   In the detection of pH value, blood gas, electrolyte and calcium ion, heparin is the only anticoagulant that can be used, and heparin lithium has the least possibility of interference in the detection of non lithium ion, so heparin lithium is recommended as anticoagulant. At present, heparin lithium is gradually replacing heparin sodium in blood test.   heparin lithium powder   It is used for biochemical test of patients after hemodialysis   The blood sample of patients after hemodialysis is a kind of special sample that many hospital laboratory departments have to face. Many inspectors often feel helpless when facing such samples. This is because hemodialysis patients use anticoagulant drugs containing heparin, such as low molecular weight heparin calcium, etc. at this time, because there is a certain amount of heparin anticoagulant drugs in the blood, it is very difficult to coagulate and separate the blood after the blood is extracted and placed in the common tube or separation gel / coagulant tube.   It has been reported that there is bias in the analysis of blood K detected by heparin lithium anticoagulant plasma and serum, which may be caused by the high rotation speed of blood sample during centrifugation, the destruction and hemolysis of red blood cells in fibrin clot, and the release of K ions in red blood cells into serum. In addition, a small amount of red blood cell damage is inevitable in the process of transportation and coagulation of blood samples, resulting in slightly high blood K.   In addition, the results of CK-MB, LDH and Glu detected by heparin lithium anticoagulant plasma and serum were significantly different, which may be due to hemolysis or decomposing blood glucose after long storage. On the contrary, heparin anticoagulant does not affect the cell volume and is not easy to cause hemolysis. It does not need to be placed in a water bath and can be directly centrifuged. The blood K, Glu and LDH measured by heparin lithium anticoagulant plasma are more close to the real concentration of patients. Therefore, establishing the reference value system of plasma biochemical test as soon as possible is helpful for clinicians to make accurate judgment on patients' condition.   Used for routine biochemical test   The results showed that heparin lithium anticoagulant plasma could be used to replace serum in most routine biochemical tests, but the reference range of Glu, K +, Na +, CI -, P3 - should be established, and the reference range of serum could be used to explain the results of plasma test in other items; LP (a), PA, HBDH, LDH, CK, CKMB, IgM and other items should not be measured with heparin lithium anticoagulant plasma samples, and the change of plasma Lp (a) with heparin concentration It increases with the increase of temperature.   Desheng has been engaged in the R & D and production of blood collection additives for 15 years. It can be said that Desheng is an old manufacturer of blood collection additives. Heparin sodium and heparin lithium are our main products. They are non injection grade. They are mainly used as anticoagulants in blood collection additives, that is, in vitro anticoagulants. They are recommended and purchased by many companies. Moreover, we can also customize heparin products with potency of 150iu-180iu according to the needs of customers.

HEPES is a kind of amphoteric buffer, which belongs to good's buffer. It is an important biochemical agent in biochemical industry and one of the important buffers in the field of biological research. HEPES buffer chemical name: N-hydroxyethyl piperazine-1-ethanesulfonic acid, white crystalline powder, is a weak acid, its pH buffer range is 6.8-8.2, soluble in water, does not form stable complexes with metal ions, in most cases, will not interfere with the biochemical process. Because of its advantages, we can find its trace in many fields.   HEPES (7365-45-9) is a cosmetic ingredient with EWG green certification, which is safe and mild. HEPES has the advantages of safety to human body, non toxicity, good physiological compatibility and stable chemical properties.   HEPES can stabilize the pH range of skin care products for a long time, better maintain the activity of microbial fermentation products extract, and make the anti-aging effect of products last for a long time, so it is often used as a stabilizer and activator in cosmetics. HEPES is also a common penetration enhancer, which can promote the transdermal absorption of various functional components in cosmetics. Compared with azone penetration enhancers, HEPES has the characteristics of short onset time, less dosage and high penetration rate. In addition, in some famous cosmetics such as L'Oreal and Lancome, HEPES is mainly used as a peeling agent to soften cutin and promote the metabolism of skin cells.     HEPES is more commonly used in cell culture, in vitro fertilization and embryo culture buffer, but HEPES can be combined with metal ions, most of the metal ion research is carried out by using HEPES buffer, although Tris and phosphate can also be used for metal ion research, but based on the characteristics of these buffers, it is necessary to choose different directions There will be some differences.   The application range of Desheng HEPES buffer is just in the range of ph6.8-ph8.2, which is in line with the characteristics of the pH value needed for cell culture. Compared with other buffers, HEPES has higher stability in the environment of maintaining the pH value of cell culture medium, so HEPES has higher stability Buffer is widely used in cell culture, tissue culture, protein purification and extraction, immunoprecipitation, cell division, living cell imaging and other biological and biochemical research.   Gold nanoparticles were prepared by HEPES NaOH reduction method based on the reducibility of HEPES to excessive metals at a specific pH value. The method is simple, fast, easy to control, less by-products and environmentally friendly.   In protein research, HEPES is often used as the component and eluent of binding buffer in cation exchange chromatography; in DNA research, HEPES is used as the buffer of calcium phosphate and DNA precipitate formation system, AFM and electroporation experiment. In addition, HEPES interferes with the reaction between DNA and restriction enzyme and is not suitable for Lowry's method. HEPES buffer is also used in chemical diagnostic kits, DNA / RNA extraction kits and PCR diagnostic kits.   There are many uses of HEPES waiting for us to discover. Anything of value is worth studying and discovering. Desheng, as a manufacturer of biological buffers, besides HEPES, has a series of buffers such as Tris, Tris HCl, bicine, caps, mops, taps, etc.

N-Tris(Hydroxymethyl)Methyl-3-Aminopropanesulfonic Acid (TAPS) is a white powder with molecular formula c7h17no6s, molecular weight 243.278, CAS number 29915-38-6, and TAPS buffer is also called tabs Buffer, a sulfonic zwitterionic buffer, is widely used in biochemistry and molecular biology. Its pH buffer range is 7.7-9.1, soluble in water (25g / 50ml) and PKA is 8.4. It is commonly used in biochemical diagnostic kit, DNA / RNA extraction kit and PCR diagnostic kit in clinical diagnosis. In biological and biochemical experiments, it is often necessary to use the medium with stable pH range, so buffer reagent is often used. The traditional buffer composed of weak acid and its salt or the mixture of weak base and its salt has many kinds, low price and easy to obtain, but it has some shortcomings, such as insufficient buffer capacity, affecting the biochemical reaction process and so on.   Sulfonic acid type biological buffer is an ideal biochemical buffer at present, which has many outstanding characteristics superior to the traditional buffer. Taps sulfonic biological buffer is often used as a buffer system in DNA screening system, and can also be used as a buffer component of RNA samples; it is suitable for electron transfer and phosphorylation research of chloroplast thin layer preparation; it can protect oxyhemoglobin from oxidation to methemoglobin during freeze-drying; it can also be used as a background electrolyte for protein microanalysis by capillary zone electrophoresis.   Desheng biochemical, as an old manufacturer of in vitro diagnostic reagents, produces taps buffers with high purity, good quality, stable buffering performance and little impact on the experimental results. In addition to taps, we can also provide other biological buffers such as Tris, HEPES, caps, mops, as well as various in vitro diagnostic reagent raw materials such as chemiluminescence reagent, chromogen substrate, blood collection vessel additive, etc.   Desheng biochemical friendly tips:   1. Protection should be done according to the situation of the test. Wear necessary protective tools, test clothes, rubber gloves, protective mask, gas mask, etc. according to the possible dangerous accidents. Before the experiment, we should pay attention to clean up the potential safety hazards around the test site. Check whether the test device, drugs and related articles do not meet the requirements.   2. Follow the nature of chemicals and the law of chemical reaction to avoid the experimental accidents caused by improper operation. The matching reaction device should be selected according to the nature and process of chemical reaction, and the necessary safety measures should not be omitted.   3. To estimate the risk of the experiment, attention should be paid to the following types of experiments: ① unknown reactions and operations; ② experiments with multiple risks (such as fire, toxic and harmful gases, etc.); ③ experiments under harsh reaction conditions (such as high temperature, high pressure, etc.).   4. Take accident prevention measures and check: be familiar with the position and operation method of switches and fire extinguishers around the experimental environment.   5. Post treatment: pay attention to the cleaning of the experimental platform, the arrangement of articles, the treatment of waste liquid, etc.

Biological buffer is a kind of solution that can keep the pH value of the solution relatively stable when a small amount of acid or alkali is added. Most cells can only carry out activities in a very narrow pH range, and need a buffer system to resist the pH changes in the process of metabolism. In biochemical research, buffer solution is often used to maintain the pH of the experimental system. Let's take a look at the commonly used biological buffer solutions and their characteristics.   Desheng buffer packaging   Phosphate buffer   Phosphate buffer is the most widely used buffer. Due to the secondary dissociation, the pH range of the buffer is wide, and the acidic, alkaline and neutral buffers with different pH values can be configured   When preparing acidic buffer solution, NaH2PO4 or KH2PO4 can be used directly, and the pH value ranges from 1 to 5; Alkaline buffer can be directly used Na2HPO4 or K2HPO4 with pH range of 9-12; The neutral buffer contains the same amount of NaH2PO4 and Na2HPO4 or the same amount of KH2PO4 and K2HPO4 solution, and the pH value is 5.5 ~ 8.5.   Advantages: ① easy to prepare into various concentrations; ② wide range of pH value; ③ little influence of temperature on pH value; Disadvantages: ① it is easy to precipitate with common calcium, magnesium and heavy metal ions; ② it inhibits some biochemical processes; Desheng Laboratory Tris buffer Tris buffer is widely used in biochemical research. It is a weak base and is usually used in the "neutral" range. In addition to tris - HCl, Tris has a variety of derivatization buffers   TBS = Tris HCl + NaCl + KCl, which is often used to clean the Western blotting membrane of immunostained tissue or Western blotting; Tbst = Tris HCl + NaCl + Tween20, a membrane buffer commonly used for Western blotting; TE = Tris HCl + EDTA, which has protective effect on DNA base and is often used for DNA stabilization and storage; Tae = Tris base + acetic acid + EDTA is a buffer system widely used in short DNA electrophoresis; Tbe = tribasic + boric acid + EDTA, suitable for long-term DNA electrophoresis, with good separation effect for small fragments   Advantages: ① because Tris base has strong alkalinity, it can only be used to prepare buffer solution with a wide range of pH from acidic to alkaline; ② it has little interference to biochemical process and does not precipitate with calcium, magnesium ions and heavy metal ions. Disadvantages: ① the pH value of the buffer solution is greatly affected by the concentration of the solution, and the pH value changes more than 0.1 when the buffer solution is diluted ten times; ② the temperature effect is large, and the temperature change has a great influence on the pH value of the buffer solution, so it must be prepared at the use temperature, and the tris HCl buffer prepared at room temperature cannot be used at 0 ℃ ~ 4 ℃. (3) it is easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed. ④ The buffer solution interferes with some pH electrodes, so the electrode compatible with tris solution should be used.   Glycine buffer   Glycine buffer has a wide range of pH value and wide range of application, ranging from 2.0 to 11.0.   Advantages: provide a closer natural environment for cell components and various extracts. Disadvantages: similar to phosphate buffer system; interferes with some biochemical processes, such as metabolism.   Desheng technology was founded in 2005. After years of innovation and development, the company has become a national high-tech enterprise with R & D, production, sales and service as a whole, which is dominated by in vitro diagnostic reagent raw materials, biological buffer, blood collection additive, color reagent and chemiluminescence reagent.

The full name of toos is n-ethyl-n - (2-hydroxy-3-sulfopropyl) - 3-methylaniline sodium salt, which is a widely used in vitro diagnostic reagent and is most commonly used in the new Trinder's reagent. Tools has been used in many clinical biochemical tests. Which tests can tools be used in?   Toos is widely used in diagnostic detection and biochemical test by Trinder reaction. Trinder reaction is also known as "coupling end-point colorimetry". The principle is that hydrogen peroxide (H2O2) produced by enzymatic reaction of the tested substance can generate red quinoline compound in the presence of 4-aminoantipyrine (4-AAP) and peroxidase (POD). Blood glucose test items: toos is used to prepare glucose test reagent and glycosylated albumin test reagent in blood glucose metabolism items.   Glucose oxidase was used to catalyze the oxidation of glucose to produce hydrogen peroxide. Platinum bismuth nanomimetic peroxidase was used to catalyze the oxidation of 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) and sodium n-ethyl-n - (2-hydroxy-3-sulfopropyl) - 3-methylaniline (toos) by hydrogen peroxide. The color of the solution was purple. With the increase of glucose concentration, the color of the solution increased, The maximum absorption wavelength of the chromogenic system is 590 nm, and the content of glucose can be obtained from the absorbance at 590 nm.   Routine examination items of liver function: adenosine deaminase (ADA) activity is a sensitive index reflecting liver injury, and it is one of routine examination items of liver function. TOOS, bovine serum albumin, disodium ethylenediamine tetraacetate and other components of adenosine deaminase detection reagent have the advantages of good color effect, rapid response, stability and high precision.   In addition, tools is also used in triglyceride and other blood lipid testing items and cholesterol testing items of diagnostic reagents, which has important value in clinical diagnosis.   "Toos" here refers to the in vitro diagnostic reagent toos. Hubei xindesheng Material Technology Co., Ltd. is a manufacturer of in vitro diagnostic reagent raw materials. In addition to providing toos, it can also provide many chromogen substrates, such as heparin lithium, heparin sodium, EDTA dipotassium / Tripotassium, and biological buffers such as Tris, Tris-HCl, HEPES, mops, etc.