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Some problems in the purchase of color reagent

2021-03-11
Some problems in the purchase of color reagent

Before purchasing color reagent, let's distinguish the difference between color reaction and color reaction. Color reaction changes the color of chemical substances through chemical reaction, while color reaction of color reagent refers to the reaction of hydrogen peroxide (H2O2) produced by the tested substances through enzymatic reaction in the presence of 4-aminotripyrine (4-AAP) and peroxidase (POD) to make the chromogenic substances produce red quinone Imine compounds, the following listed under the purchase of color reagents need to pay attention to several issues.

 

Pay attention to the parameters of different reagents

Chromogenic reagents usually have various parameter values, such as molar absorbance, color reaction sensitivity, maximum absorption wavelength, purity, etc. the detection principle of chromogenic substrate is to use spectrophotometry to measure the absorbance change at a specific maximum absorption wavelength before and after reaction, so as to analyze the concentration of the substance to be measured. Therefore, it is necessary to select different types of chromogenic reagents We must make clear the requirements of the laboratory for each parameter.

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How to choose developer manufacturer

There is no big difference for the commonly used reagents required by the manufacturer, but for those that are not commonly used, especially those with high sensitivity demand such as chromogenic reagents, the manufacturer should choose the direct manufacturer rather than the foreign trader, the manufacturer with R & D and production capacity, and the manufacturer with standard and complete packaging should not choose the bulk self filling. Choose a formal purchase platform or channel, so that it will not affect the process of scientific research.

 

It's easy to ignore the purity level required by the reagent. In some reactions, there will be great differences. Here are some common reagent levels, GR: superior purity. It is suitable for accurate analysis and research, and some can be used as reference materials.

 

Ar: analytical pure. It is suitable for industrial analysis and chemical experiments.

 

CP: chemical purity. It is suitable for chemical experiment and synthesis.

 

LR: experimental pure. It is only suitable for general chemical experiments and synthetic preparation.

 

Br: biochemical reagent. It is used for preparing biochemical test solution and biochemical synthesis. Quality indicators focus on bioactive impurities. It can replace indicator and organic synthesis.

 

BS: biological dye. It is suitable for preparing the staining solution of biological samples. Quality indicators focus on bioactive impurities. It can replace indicator and organic synthesis.

 

Desheng has been committed to the development and production of in vitro diagnostic reagents. There are a wide range of color reagents, including toos, tops, ADOS, ADPS, Alps, Daos, hdaos, MADB and other products. Compared with the color reagent products of other manufacturers, the reaction reagent is shorter, the accuracy is higher, and the accurate diagnosis can be made more quickly in the experiment.