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Latest company new about Do you know how to judge the quality of serum separation gel?
2024/07/12

Do you know how to judge the quality of serum separation gel?

Judging the quality of serum separation gel is a crucial step in clinical testing and biological research, as it directly affects the efficiency of blood sample processing and the accuracy of results. Serum separation gel, as a special additive, is widely used in vacuum blood collection tubes. Its main function is to form an isolation membrane during centrifugation to separate serum from blood clots, thereby protecting serum components from contamination by cellular components and maintaining sample stability. The following is a detailed explanation of how to determine the quality of serum separation gel from multiple dimensions. 1.Thixotropic properties and viscosity The thixotropy of serum separation gel is one of its core characteristics, which determines the flow behavior of the gel under different external forces. The ideal separation gel should reduce its viscosity due to force during centrifugation, making it easier to pass through the blood cell layer and form a stable isolation layer. Once centrifugation stops, the viscosity quickly recovers, maintaining the isolation effect. Separation gel with poor thixotropy may result in poor flipping or formation of a uniform isolation layer, affecting the purity of serum. Meanwhile, viscosity is another key indicator that not only affects the smoothness of the gluing process, but also determines the flowability of the colloid during centrifugation. Excessive or insufficient viscosity can cause inconvenience to operation or affect separation efficiency. Although most manufacturers do not explicitly indicate viscosity, good separation adhesives should have suitable and stable viscosity values to ensure the convenience and effectiveness of adding adhesive. 2.Specific gravity and separation effect The specific gravity of serum separation gel is usually designed between 1.045 and 1.065g/cm ³, which ensures that the separation gel can be located between blood cells and serum after centrifugation, forming a good physical barrier. Separation gel with a specific gravity that does not meet the requirements may not be able to accurately separate layers, leading to the infiltration of blood cell components into the serum and affecting subsequent test results. In addition, for specific applications such as PRP (platelet rich plasma) collection, the specific gravity of the separation gel needs to be accurately matched to achieve finer separation.   3、 Shelf life and stability A good serum separation gel should have good long-term stability, and even under specified storage conditions (such as avoiding light, room temperature, or refrigeration), there should be no aging or solidification phenomenon. The shelf life of products from different manufacturers varies, generally ranging from one to three years. However, regardless of the duration, the key is that during this period, the thixotropy, viscosity, and specific gravity of the separation adhesive should remain unchanged. Long term stability testing is an important basis for assessing its quality, ensuring that even after prolonged use, the original characteristics of the blood sample are not compromised. 4、 Sample processing capability The key to testing the efficacy of serum separation gel is whether it can quickly form a stable isolation layer upon contact with blood, and whether it can maintain the original characteristics of the sample over a long period of time. In clinical practice, if the quality of the separation gel in the blood collection tube is poor and the specific gravity does not meet the standard, it may lead to the mixing of serum and blood cell components, and even the appearance of fibrin fibers in the serum, which usually means poor blood coagulation or improper centrifugation conditions. Therefore, a good separation gel should be able to quickly and coagulate blood, avoid contamination of serum by fibrin and other cellular components, and ensure the reliability of analysis results. 5、 Anti interference and compatibility High quality serum separation gel should also have good anti-interference ability, which means it can maintain stable separation effect under various sample conditions, without being affected by sample type, blood composition changes, or external factors such as temperature fluctuations. In addition, compatibility is also important as it should be compatible with various conventional centrifugal equipment, automated analytical instruments, and subsequent biochemical detection methods without affecting the test results.   In summary, when selecting serum separation gels, laboratories and medical institutions should comprehensively evaluate the above factors to ensure that the selected products can meet experimental requirements, improve detection efficiency and accuracy, and provide reliable data support for clinical diagnosis and scientific research. Desheng is a professional manufacturer of serum separation gel, with an independent research and development team and strict quality control by professional personnel. If you have purchasing needs, please feel free to contact us for purchase!
Latest company new about Application of Bicine in the Preparation of Stable Substrate Solutions
2024/07/11

Application of Bicine in the Preparation of Stable Substrate Solutions

Application of Bicine in the Preparation of Stable Substrate Solutions The stability of substrate solutions is crucial for the accuracy of experimental results in biochemistry and molecular biology experiments. Among them, Bicine (N, N-di (hydroxyethyl) glycine) is highly favored as a biological buffer due to its excellent properties, playing an indispensable role in preparing stable substrate solutions. This article will discuss in detail the application of Bicine in the preparation of stable substrate solutions from multiple aspects.     The basic characteristics of Bicine Bicine, Also known as N, N-di (hydroxyethyl) glycine, it is a zwitterionic buffer and belongs to the Good's buffer series. Its CAS number is 150-25-4, and its pH buffer range is between 7.6-9.0. As a biological buffer, Bicine is widely used in low-temperature biochemical work, especially in the preparation of stable substrate solutions. Application of Bicine in the Preparation of Stable Substrate Solutions 1. Enzyme reaction buffer In enzymatic reactions, enzymes act as biocatalysts and their activity is greatly influenced by pH values. Bicine, as an enzyme reaction buffer, can maintain pH stability within a certain range, providing a suitable environment for enzymatic reactions. Meanwhile, Bicine has strong buffering ability and can resist external acid-base interference, ensuring the stability of the substrate solution. 2. Serum guanine enzyme determination In the experiment of serum guanine enzyme determination, the stability of the substrate solution is crucial for the accuracy of the measurement results. Bicine, as a biological buffer, can effectively stabilize substrate solutions and prevent them from changing during the measurement process. Meanwhile, Bicine can also interact with other components in the serum, improving the sensitivity and accuracy of the measurement. 3. Electrophoretic buffer Electrophoresis buffer is an important part of nucleic acid and protein gel electrophoresis system, which is of great significance for maintaining a constant pH value of the electrophoresis system. Bicine, as an electrophoresis buffer, can provide a stable pH environment for biological macromolecules such as nucleic acids and proteins during the electrophoresis process, ensuring their stability during the electrophoresis process. In addition, Bicine's buffering ability can also resist external acid-base interference, ensuring the accuracy of electrophoresis results. Preparation method of Bicine The preparation method of Bicine is relatively simple, usually requiring the preparation of Bicine powder and deionized water. Firstly, dissolve Bicine powder in deionized water in a certain proportion to prepare a certain concentration of Bicine solution. Then, according to experimental needs, the pH value of Bicine solution can be adjusted by adding alkaline solutions such as NaOH. It should be noted that during the preparation process, factors such as temperature, stirring speed, and mixing ratio should be strictly controlled to ensure the quality and stability of Bicine solution. conclusion In summary, Bicine plays an important role as an excellent biological buffer in the preparation of stable substrate solutions. Its pH buffering ability and stability make it an ideal choice for enzymatic reactions, serum guanine enzyme determination, and electrophoresis buffer solutions. With the continuous deepening of research in biochemistry and molecular biology, the application prospects of Bicine will be even broader. As a supplier of Bicine buffer, Desheng always adheres to the pursuit of quality and continuous exploration of innovation. Its Bicine raw materials are not only of superior quality and stable performance, but also suitable for various fields. As a customer-centric manufacturer, Desheng understands the importance of customer needs and provides a wide range of buffer products, from commonly used specifications to customization, ensuring that customers can find solutions that meet their needs here. At the same time, Desheng also provides professional technical support and after-sales service, allowing customers to enjoy a more convenient shopping experience. If you have any relevant intentions, please feel free to click on the website to inquire about details and purchase at any time!
Latest company new about The application of the new Trinder's reagent TOOS in blood glucose and liver function detection
2024/07/10

The application of the new Trinder's reagent TOOS in blood glucose and liver function detection

With the continuous progress of biotechnology and medical technology, biochemical testing plays an increasingly important role in clinical diagnosis and treatment. Blood glucose and liver function testing, as important components of biochemical testing, have profound significance in evaluating human health status, monitoring disease progression, and guiding clinical treatment. In recent years, the new Trinder's reagent TOOS (N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt) has gradually demonstrated its unique advantages and application value as a new biochemical detection reagent in blood glucose and liver function detection. This article will explore in detail the application of TOOS in blood glucose and liver function detection. The basic principle of the new Trinder's reagent TOOS TOOS is an organic compound with specific chemical structures and reaction properties. In biochemical testing, TOOS is usually used as a reaction substrate to undergo specific chemical reactions with the tested substance, generating colored products. The generation amount of this colored product is directly proportional to the concentration of the tested substance, so the concentration of the tested substance can be indirectly determined by measuring the absorbance, fluorescence intensity, and other parameters of the colored product. The advantages of TOOS lie in its high sensitivity, strong specificity, simple operation, and strong anti-interference ability, making it widely used in blood glucose and liver function detection. The application of the new Trinder's reagent TOOS in blood glucose detection Blood glucose is the concentration of glucose in the blood and an important diagnostic indicator of metabolic diseases such as diabetes. Traditional blood glucose detection methods such as glucose oxidase method, although mature and stable, may be interfered with in some cases, affecting the accuracy of the results. The application of the new Trinder's reagent TOOS in blood glucose detection provides a new and more accurate method for blood glucose detection. In the TOOS method, glucose undergoes an oxidation-reduction reaction with the TOOS reagent to produce colored products. The amount of this colored product is directly proportional to the concentration of glucose, so blood glucose concentration can be accurately measured by measuring parameters such as the absorbance of the colored product. Compared with traditional glucose oxidase methods, TOOS method has higher sensitivity and specificity, and can more accurately reflect blood glucose levels. In addition, the TOOS method has the advantages of simple operation, fast reaction speed, and strong anti-interference ability, making blood glucose detection more rapid, accurate, and reliable.   The application of the new Trinder's reagent TOOS in liver function detection Liver function testing is an important means of evaluating liver function status, including multiple indicators such as transaminase, bilirubin, albumin, etc. The new Trinder's reagent TOOS has also played an important role in liver function testing. In transaminase detection, TOOS can act as a substrate to react with transaminases and generate colored products. By measuring the absorbance and other parameters of colored products, the activity of transaminases can be indirectly measured, thereby evaluating the metabolic function of the liver. Compared with traditional colorimetric methods, the TOOS method has higher sensitivity and specificity, and can more accurately reflect changes in transaminase activity. In addition, TOOS can also be used for the detection of indicators such as bilirubin and albumin. Bilirubin is one of the important indicators of liver function, and its concentration changes can reflect the excretion function of the liver. In bilirubin detection, TOOS can react with bilirubin to generate colored products, thereby indirectly determining the concentration of bilirubin. Albumin is an important protein synthesized by the liver, and changes in its concentration can reflect the synthesis function of the liver. In albumin detection, TOOS can react with albumin as a substrate to generate colored products. By measuring the absorbance and other parameters of the colored products, the concentration of albumin can be indirectly measured. The application advantages of the new Trinder's reagent TOOS 1. High sensitivity and specificity: TOOS can react specifically with the tested substance, generate colored products, and accurately determine the concentration of the tested substance, thereby improving the sensitivity and specificity of detection. 2. Easy to operate: TOOS has simple and practical operation steps, without the need for complex instruments and cumbersome operation processes, reducing the difficulty and cost of detection. 3. Fast reaction speed: TOOS has a fast reaction speed and can complete detection in a short time, improving detection efficiency. 4. Strong anti-interference ability: TOOS has strong anti-interference ability, which can reduce the influence of external factors on the detection results and ensure the accuracy of the detection results. The new Trinder's reagent TOOS has demonstrated its unique advantages and application value in blood glucose and liver function detection. Its high sensitivity, specificity, simplicity, fast reaction speed, and strong anti-interference ability have made the TOOS method widely used in the field of biochemical detection. With the continuous progress of medical technology and the continuous innovation of biochemical detection methods, the new Trinder's reagent TOOS is expected to play a greater role in the future, providing more accurate, fast, and convenient detection methods for clinical diagnosis and treatment. Desheng is a manufacturer of new Trinder's reagents and can provide a series of reagents such as TOOS. Independently developed and produced, with small inter batch differences and sensitive reactions, it has important value in clinical diagnosis. If you need to make a purchase, please feel free to contact us for consultation and ordering!
Latest company new about Exploration of the Reasons for Fading of MAOS Display Reagents
2024/07/08

Exploration of the Reasons for Fading of MAOS Display Reagents

In the field of chemical analysis and diagnostic testing, colorimetric reagents play a crucial role. Among them, MAOS, as a new type of Trinder's reagent, has received widespread attention due to its high water solubility and color performance. However, MAOS reagents often exhibit fading during use, which not only affects the accuracy of testing but also brings many inconveniences to experimental operations. This article will delve into the reasons why MAOS reagents are prone to fading from multiple perspectives.   1、 Chemical properties of MAOS reagents The full name of MAOS is N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3,5-dimethylaniline sodium salt, which is a derivative of highly water-soluble aniline sodium salt. These substances will produce red or yellow substances after oxidation, thereby achieving color rendering function. However, the chemical properties of MAOS reagents also determine their tendency to fade. Due to the presence of easily oxidizable groups in MAOS molecules, oxidation reactions can occur when exposed to oxygen or other oxidants in the air, leading to a gradual fading of color. So when using MAOS, it is important to detect it in a timely manner and not interrupt the process. 2、 The impact of storage environment The storage environment of MAOS reagents has a significant impact on their stability. Firstly, temperature is a key factor affecting the stability of reagents. Excessive temperature can accelerate the oxidation reaction of MAOS molecules, leading to reagent discoloration. Therefore, when storing MAOS reagents, they should be placed in a cool and dry environment to ensure that the reagents are not affected by the external environment during storage. In addition, humidity is also an important factor affecting the stability of reagents. Excessive humidity can cause reagents to absorb moisture and clump, reduce their effectiveness, and even accelerate oxidation reactions. Therefore, when storing MAOS reagents, the environment should be kept dry to avoid high humidity. 3、 Precautions during operation In addition to the storage environment, precautions during operation also have a significant impact on the stability of MAOS reagents. Firstly, when preparing the MAOS reagent solution, it is necessary to strictly follow the instructions and configure the appropriate concentration. Excessive concentration may accelerate the oxidation rate of the reagent, while low concentration may affect the color rendering effect. Secondly, when using MAOS reagents, the operating environment should be kept clean and tidy to reduce the interference of impurities and pollutants. At the same time, it is advisable to avoid mixing test reagents with other chemical substances to avoid chemical reactions that may affect the detection results. Finally, the stability of MAOS reagents after opening is also a concern. Once the reagent is opened, its stability may be affected by factors such as oxygen and moisture in the air, so it should be used up as soon as possible after opening. If long-term storage is required, it should be resealed and placed in an appropriate storage environment. In summary, the main reasons for the easy fading of MAOS reagents include their chemical properties, storage environment, precautions during operation, and the lack of effective stability improvement methods. To solve this problem, we need to start from multiple aspects, optimize the storage environment, improve packaging materials, pay attention to operational details, and explore effective methods to improve stability. Only in this way can the accuracy and reliability of MAOS reagents be ensured in chemical analysis and diagnostic testing. The MAOS manufactured by Desheng is highly praised for its purity, water solubility, highly sensitive color rendering ability, and manufacturing process. The product ensures a pure white crystalline powder appearance, which is loved and recognized by users both domestically and internationally. If you are looking for a new type of The New Trinder's reagent, we sincerely invite you to visit the official website of Desheng for more detailed information.
Latest company new about Comparative study of anticoagulant tube heparin lithium and EDTA tube in blood sample collection
2024/07/05

Comparative study of anticoagulant tube heparin lithium and EDTA tube in blood sample collection

Blood sample collection is an important part of modern medical diagnosis, and the selection of anticoagulants is crucial for ensuring sample quality, maintaining cell integrity, and ensuring accuracy in subsequent analysis. Among numerous anticoagulants, heparin lithium and EDTA (ethylenediaminetetraacetic acid) are the two commonly used, each with its own characteristics and suitable for different types of blood testing. This article aims to compare and study the application characteristics, advantages and disadvantages, and scope of application of lithium heparin tubes and EDTA tubes in blood sample collection, in order to provide reference for clinical practice and laboratory research. 1、 Anticoagulant mechanism and chemical properties Heparin lithium is a natural polysaccharide anticoagulant extracted from animal tissues. It mainly enhances the activity of antithrombin III, thereby inhibiting the activity of thrombin and other coagulation factors, achieving the goal of anticoagulation. Compared to other salt forms such as sodium and ammonium salts, heparin lithium has better water solubility and stability, making it more suitable for whole blood cell analysis and certain biochemical tests. Due to its ability to interact with various proteins, heparin may interfere with certain blood tests, especially those that rely on calcium ions for coagulation function testing. EDTA is a synthetic chelating agent that combines with calcium ions in the blood to form a stable complex, thereby preventing calcium ions from participating in the coagulation process and achieving anticoagulant effects. EDTA-K2 (potassium ethylenediaminetetraacetate) is a commonly used form of EDTA salt, suitable for routine hematological tests such as blood cell counting and classification, as it has a relatively small impact on the morphology and quantity of blood cells. However, the inhibitory effect of EDTA on platelet function limits its application in testing that requires evaluation of platelet function.   2、 Application scope and advantages Lithium heparin tubes are particularly suitable for blood gas analysis, hematocrit tests, and some biochemical tests due to their mild and stable anticoagulant effects. Heparin lithium can effectively maintain the original state of blood components, reducing possible cellular damage and metabolic changes during sample processing. Therefore, it is more advantageous for immediate detection (POCT) and testing items that require quick results in emergency situations. However, it should be noted that heparin has an aggregation effect on white blood cells and is not suitable for white blood cell counting and classification, and may interfere with the determination of specific enzyme activity. EDTA tubes are widely used in hematological tests, such as whole blood cell count (CBC), white blood cell classification, and red blood cell morphology, due to their ability to prevent blood clotting. The anticoagulant effect of EDTA is stable and reliable, and it has little impact on the morphology of blood cells. It is the standard anticoagulant for blood cell counting. However, the samples collected by EDTA tubes are not suitable for coagulation function testing, as the chelation of calcium ions can affect the determination of indicators such as prothrombin time (PT) and activated partial thromboplastin time (APTT). 3、 Comparison and selection When selecting anticoagulants, it is necessary to clarify the testing purpose. If the detection focuses on blood gas analysis, biochemical indicators (non calcium dependent), or needs to maintain the physiological activity of blood components, heparin lithium tubes are the preferred choice. When the focus of detection is on routine hematological indicators, especially when counting and classifying blood cells, EDTA tubes are more suitable. It is worth noting that for situations where both blood cell count and biochemical testing are required, separate sample collection or the use of dual anticoagulant tubes should be considered to avoid mutual interference between anticoagulants affecting the detection results. In summary, the applications of lithium heparin tubes and EDTA tubes in blood sample collection have their own advantages, and selecting appropriate anticoagulants is the key to ensuring the accuracy of test results. Clinical doctors and laboratory technicians need to fully understand the characteristics of various anticoagulants, select anticoagulant tubes reasonably based on specific testing needs, optimize sample processing flow, and improve testing quality and efficiency. In the future, with the development and application of new anticoagulants, blood sample collection technology will become more refined, providing more possibilities for medicine. As a raw material supplier in the biochemical market, Desheng provides high-quality heparin lithium, EDTA K2 anticoagulants, and other blood collection additives, all at a low price. If you have any relevant intentions, please click on the website to inquire about details and purchase!
Latest company new about What is the appropriate pH value for HEPES to cultivate 293T cells?
2024/07/03

What is the appropriate pH value for HEPES to cultivate 293T cells?

In cell biology and molecular biology research, 293T cells, as a transformed human embryonic kidney cell line, have become a popular model system in scientific research due to their easy transfection and good expression of exogenous genes. To ensure that these cells can grow healthily and maintain their biological characteristics, accurate control of culture conditions is crucial, with pH stability being one of the foundations. HEPES buffer(4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid) plays an irreplaceable role as a good buffer in maintaining pH stability in cell culture media. This article will delve into the appropriate pH range of HEPES and its application strategies in cell culture when cultivating 293T cells.   The role and importance of HEPES HEPES is an excellent organic buffering agent that can maintain pH stability of solutions over a wide pH range (usually 6.8 to 8.2). It does not interfere with most biochemical reactions and does not form stable complexes with metal ions, making it widely used in cell culture, enzyme activity research, and protein expression. For 293T cells, the addition of HEPES helps to reduce pH fluctuations caused by metabolic activity, ensuring that the cells are in a suitable growth environment. Ideal pH environment for 293T cells Although HEPES itself has a wide buffering capacity, the pH setting of the culture medium for 293T cells still needs to consider the physiological preferences of the cells. Research has shown that the 293 cell line (including 293T) prefers a slightly acidic environment, and pH values between 6.9 and 7.1 can promote cell adhesion and growth. However, in practical operation, considering the buffering effect of HEPES and the universality of cell culture media, it is generally recommended to adjust the pH value of the culture medium to between 7.0 and 7.2. This not only satisfies the preference of cells for a slightly acidic environment, but also ensures the effectiveness of the HEPES buffer system, avoiding cell growth inhibition or morphological abnormalities caused by alkalinity. Adjustment strategies in practice 1. The amount of HEPES added: According to the experimental requirements, adding an appropriate amount of HEPES can effectively buffer the acidic substances produced by cell metabolism during cell culture and maintain pH stability. 2. Using NaHCO3 to adjust pH: Although HEPES is the main buffering component, the addition of NaHCO3 to the culture medium is also necessary to preliminarily adjust the pH value to the target range. It should be noted that the purity and manufacturer differences of NaHCO3 from different batches may lead to slight changes in pH value. Therefore, when using a new batch of NaHCO3, a pH meter should be calibrated to ensure that the pH of the culture medium falls between 7-7.2. 3. Regular monitoring and adjustment: During the cell culture process, the pH value of the culture medium should be regularly monitored and fine tuned as needed. Although HEPES provides strong buffering ability, prolonged cell culture or high-density cell culture may cause pH to gradually shift. Timely replacement of fresh culture medium or appropriate addition of HEPES buffer is the key to maintaining a good culture state. 4. Personalized adjustment: The experimental conditions in different laboratories (such as carbon dioxide concentration, temperature, etc.) and the number of cell passages may affect the sensitivity of cells to pH, so it is essential to make personalized adjustments according to the actual situation while following basic guiding principles.   In summary, understanding and controlling the appropriate pH range of HEPES is a fundamental and important step in nurturing 293T cells. By properly allocating the use of HEPES and NaHCO3, combined with regular monitoring and adjustment, a growth environment close to the ideal state can be created for 293T cells, thereby improving the reliability of the experiment and reducing reproducibility. Remember, cell culture is both a science and an art, and subtle adjustments can often lead to significant improvements in experimental results. As an advantageous manufacturer of HEPES biological buffering agents, Desheng can supply raw materials with 99% purity for manufacturers to prepare and use, which is convenient, simple, and has stable buffering performance. If you have any relevant intentions, please feel free to inquire for details!
Latest company new about Optimizing the accuracy of blood gas analysis: advantages and effects of heparin lithium anticoagulants in application
2024/07/01

Optimizing the accuracy of blood gas analysis: advantages and effects of heparin lithium anticoagulants in application

Blood gas analysis, as an important diagnostic tool in clinical medicine, has irreplaceable value in evaluating the respiratory function, acid-base balance, and electrolyte levels of patients. In this precise detection process, the selection of anticoagulants is crucial, and heparin lithium plays a core role in the field of blood gas analysis due to its advantages. This article will explore in detail the application and importance of heparin lithium in blood gas analysis from several aspects. Characteristics of heparin lithium Heparin lithium is a chemical substance with good chemical and physical properties. Its conductivity is low, the number of nuclear charges is small, and the number of electron layers is small, which makes it more stable and less likely to react with components in the blood. Secondly, the anticoagulant effect of heparin lithium is stable and not affected by temperature and pH value, which can ensure the accuracy of blood gas analysis. In addition, heparin lithium has a relatively small impact on calcium ion concentration, which can maintain stable calcium ion concentration in the blood and improve the reliability of blood gas detection.   The importance of blood gas analysis 1. Respiratory function assessment Blood gas analysis directly reflects the efficiency of pulmonary gas exchange. By monitoring partial pressure of oxygen (PaO2) and partial pressure of carbon dioxide (PaCO2), doctors can quickly determine whether patients have problems such as hypoxia or excessive ventilation, providing a basis for the diagnosis of respiratory failure and other diseases. 2. Acid base balance monitoring Blood gas analysis can also evaluate the acid-base balance of body fluids. It can help identify metabolic or respiratory acid-base imbalance through pH value, bicarbonate (HCO3 ^ -) concentration, residual base (BE) and other parameters, which is critical to the management of critical care, renal dysfunction, diabetes ketoacidosis and other conditions. The application of heparin lithium in blood gas analysis 1. Improve sample quality When collecting blood gas samples, adding lithium heparin as an anticoagulant can prevent platelet aggregation and red blood cell rupture, maintain the original state of the sample, and ensure the authenticity and repeatability of the test results. This is both the foundation and guarantee for blood gas analysis that requires high accuracy. 2. Promote real-time analysis Due to the excellent anticoagulant performance of heparin lithium, collected blood samples can be directly analyzed on the machine without waiting, greatly reducing diagnostic time and making it particularly crucial for emergency treatment of acute patients. This is particularly important in fast-paced environments such as ICUs and emergency departments. 3. Reduce operational complexity The use of heparin lithium pre charged blood collection vessels simplifies the pre-processing steps of blood gas analysis, reduces the operational burden and error rate of medical staff, and improves work efficiency and accuracy.     Advantages of heparin lithium in blood gas analysis 1. Reduce detection interference: Lithium heparin, as an excellent anticoagulant, is characterized by minimal interference with blood components. Compared to heparin sodium, heparin lithium has a smaller impact on the morphology and membrane integrity of red blood cells, significantly reducing the risk of hemolysis and ensuring the accuracy of blood gas analysis results. This low interference is particularly important for accurately measuring oxygen partial pressure, carbon dioxide partial pressure, and calculating other blood gas parameters. 2. Maintain sample stability: Blood gas analysis requires samples to be fresh and free of clots. Lithium heparin can quickly and continuously prevent the blood coagulation process, so that the collected blood samples maintain their biochemical characteristics unchanged before transportation and analysis. This feature is particularly crucial for quickly obtaining reliable diagnostic data in emergency situations. Conclusion In summary, the application of heparin lithium in blood gas analysis is not only based on its anticoagulant performance, but also on its ability to maintain sample stability. It serves as a bridge between clinical needs and laboratory testing, enabling rapid diagnosis of respiratory system and acid-base balance disorders. With the development of technology, the application of heparin lithium in blood gas analysis will become more widespread, contributing to improving the quality and efficiency of clinical diagnosis and treatment. Desheng Biochemical is a manufacturer specializing in the production of heparin sodium and heparin lithium for in vitro anticoagulants. Since its establishment in 2005, it has more than ten years of rich research and testing experience, and can effectively control the quality and efficacy of heparin. The manufacturer of the production source not only guarantees quality, but also has a very competitive price. Friends who need it can contact customer service on the Desheng official website for ordering!
Latest company new about Why is EDTA K2 anticoagulant used instead of heparin for blood routine vacuum blood collection?
2024/06/28

Why is EDTA K2 anticoagulant used instead of heparin for blood routine vacuum blood collection?

In modern medical testing, blood analysis is an indispensable part of diagnosing diseases, and blood routine examination, as the basic blood testing item, its accuracy directly affects subsequent medical decisions. To ensure the reliability of the test results, blood samples need to be quickly processed after collection to prevent coagulation, which requires the use of anticoagulants. Among numerous anticoagulants, EDTA K2 (potassium ethylenediaminetetraacetate) is widely used in blood routine vacuum blood collection, rather than heparin or other types of anticoagulants, for scientific and specific reasons behind this.   The anticoagulant mechanism of EDTA K2 EDTA K2 is a calcium ion chelating agent, which means it can tightly bind with calcium ions in the blood to form stable complexes. Calcium ions play a crucial role in the process of blood coagulation, serving as a key cofactor in the activation of prothrombin to thrombin, which further catalyzes the conversion of fibrinogen to fibrin, forming blood clots. When EDTA K2 binds to calcium ions, it effectively reduces the concentration of free calcium ions, thereby hindering the activation of prothrombin and subsequent coagulation cascade reactions, achieving anticoagulant goals. Every 0.8mg of EDTA K2 can anticoagulate 1ml of blood, making it both economical and efficient to use. Reasons for choosing EDTA K2 instead of heparin 1. The impact on blood cell morphology is relatively small: Heparin, as another commonly used anticoagulant, although has good anticoagulant effects in many situations, may cause phenomena such as white blood cell aggregation and changes in red blood cell morphology, affecting the accuracy of blood cell counting, especially the impact on white blood cell counting and classification is more significant. EDTA K2 has a better protective effect on the morphology of blood cells, which can better maintain the natural state of cells and ensure the accuracy of blood routine test results. 2. The interference of EDTA K2 on detection items is small: EDTA K2 mainly affects detection items that rely on calcium ions, such as the determination of mineral content such as calcium and magnesium, while it hardly interferes with common detection items such as red blood cell count, white blood cell count, platelet count, and hemoglobin content in blood routine. In contrast, heparin may affect the determination of certain biochemical indicators, such as the results of certain enzyme activity measurements. Therefore, when multiple tests are required, EDTA K2 becomes a better anticoagulant. 3. Convenience of operation: Blood routine testing usually requires fast and accurate detection. The use of EDTA K2 is simple, and its vacuum blood collection vessel design can ensure accurate blood collection and instant mixing, reducing operational errors and improving detection efficiency. In summary, the reason why EDTA K2 has become an anticoagulant for blood routine vacuum blood collection is because it can effectively and specifically prevent blood coagulation, while having little impact on blood cell morphology and minimal interference with routine testing items, and has good safety and operational convenience. These characteristics together ensure the accuracy and efficiency of blood routine testing, providing reliable data support for clinical diagnosis. In the modern pursuit of accurate medicine, EDTA K2 plays an irreplaceable role in the field of blood routine testing due to its advantages. As a raw material supplier in the biochemical market, Desheng provides high-quality EDTA K2 anticoagulants and other blood collection additives, which are comprehensive and affordable. If you have any relevant intentions, please click on the website to inquire about details and purchase!
Latest company new about What chemical reagent is HEPES?
2024/06/26

What chemical reagent is HEPES?

In the vast fields of biochemistry and cell biology, chemical reagents play a crucial role. They not only assist in the smooth progress of experiments, but also directly affect the accuracy and repeatability of experimental results. HEPES, The full name is 4-hydroxyethylpiperazine-1-ethanesulfonic acid, which is an indispensable chemical reagent that occupies a place in scientific research due to its buffering performance and wide applicability. Structure and properties HEPES is an organic compound with the molecular formula C8H18N2O4S, with a molecular weight of 238.3 Daltons. It belongs to the category of ionic zwitterionic buffering agents, which contain both acidic (carboxyl) and alkaline (amino) groups in the molecular structure. This characteristic enables HEPES to resist the interference of external acids and bases in certain pH ranges and maintain relative stability of the solution pH. HEPES forms a colorless and transparent solution after dissolution in water, with a pH range typically between 6.8 and 8.2. It is particularly suitable for simulating pH values under physiological conditions, making it an ideal buffer medium for cell culture, enzyme activity measurement, and various biochemical reactions. Application area 1. Cell culture In the field of cell culture, HEPES is a key component in maintaining pH stability of the culture medium. When cells are cultured in vitro, their metabolic activity produces acidic or alkaline substances, leading to pH fluctuations in the culture medium. HEPES can effectively buffer these changes, ensuring that cells grow in a pH environment close to physiological conditions, thereby improving cell health and proliferation. In addition, for cell lines that are intolerant to high concentrations of carbon dioxide, the HEPES buffer system can replace traditional bicarbonate buffer systems, reducing dependence on high CO2 incubators. 2. Biochemical experiments and reagent kits In biochemical experiments, HEPES is widely used for enzyme activity detection, protein purification, electrophoresis experiments, etc., as it can significantly improve the stability and repeatability of experiments. In clinical diagnostic test kits, HEPES, as a buffer, can ensure the stability of the reagents and the accuracy of detection results, especially in kits that require long-term storage or transportation, its buffering effect is particularly prominent. Safety and environmental protection considerations Although HEPES has broad application value in biochemical research, its use also requires attention to potential safety issues. High concentrations of HEPES may exhibit toxicity under certain cell culture conditions, affecting cell growth or causing changes in cell morphology. In summary, as an important biological buffer in biochemical research, HEPES has demonstrated its irreplaceable value in multiple fields such as cell culture and biochemical analysis. With the continuous progress of biotechnology and the increasing demand for environmental protection, research on the application efficiency and environmental impact assessment of HEPES will continue to deepen, promoting its wider and more efficient application in the field of biological science in the future. HEPES is not only a common chemical reagent in the laboratory, but also an important bridge connecting basic scientific research with clinical applications, theoretical exploration, and technological innovation. Hubei Xindesheng Material Technology Co., Ltd. was established in 2005 and is a leading manufacturer of HEPES buffering agents. It has a large-scale factory and a production research and development team. Professional personnel are available for pre-sales, during sales, and after-sales service, providing customers with full price protection throughout the entire process. The purity of the HEPES buffer currently produced can reach over 99%, with strict quality control. If you are interested, please feel free to inquire and purchase.
Latest company new about Chemiluminescence detection of luminol and iron ions: principles and application analysis
2024/06/24

Chemiluminescence detection of luminol and iron ions: principles and application analysis

Luminol, also known as luminescent ammonia, is a chemical reagent that can emit light under specific conditions. In iron ion detection, its application is mainly based on its oxidation reaction and fluorescence emission phenomenon. Specifically, in alkaline media, luminol reacts with oxygen to produce an unstable intermediate product. When this intermediate product comes into contact with iron ions, it rapidly undergoes an oxidation reaction, generating excited state Luminol molecules. Excited Luminol molecules release energy upon returning to the ground state, which is emitted in the form of fluorescence. This article will elaborate on the principle, method, application advantages, and challenges faced by Luminol in detecting iron ions.   The principle of detecting iron ions with Luminol The principle of detecting iron ions with Luminol is mainly based on its specific reaction with iron ions in alkaline media. Under alkaline conditions, luminol reacts with oxygen to produce an unstable intermediate product. When this intermediate product comes into contact with iron ions, it rapidly undergoes an oxidation reaction, generating excited state luminol molecules. Excited Luminol molecules release energy upon returning to the ground state, which is emitted in the form of fluorescence.   Luminol detection method for iron ions 1. Sample preparation: It is necessary to prepare the industrial sample to be tested, ensuring that the sample does not contain any other substances that can interfere with the detection. For complex industrial samples, appropriate pretreatment may be necessary to remove impurities. 2. Preparation of Luminol solution: Dissolve an appropriate amount of Luminol powder in alkaline buffer to obtain a suitable concentration of Lumino solution. During the preparation process, excessive addition of luminol or alkaline substances should be avoided to avoid affecting the accuracy of the test results. 3. Mixing sample with luminol solution: Mix the test sample with luminol solution and measure the fluorescence signal intensity in the mixed solution using a fluorescence meter or other suitable instrument. 4. Data analysis: Measure the iron ions in the sample based on the intensity of the fluorescence signal. Standard curve method or other suitable data processing methods can be used for data analysis.   Application advantages of Luminol in detecting iron ions 1. High sensitivity: Lumino fluorescence reaction has high sensitivity and can detect extremely low concentrations of iron ions. This makes the method of trace monitoring of iron ions important in industrial production. 2. Good selectivity: Luminol fluorescence reaction has specific recognition ability for iron ions and can accurately detect the content of iron ions in complex samples. Compared with other metal ions, luminol has stronger selectivity towards iron ions, reducing the influence of interference factors. 3. Easy to operate: Luminol fluorescence method does not require complex sample pretreatment and instrument operation, and can complete the detection process in a relatively short time. This makes the method highly practical and convenient in industrial production. 4. Low cost: As a commonly used chemiluminescent reagent, luminol has a relatively low price and a simple preparation process. This makes the Luminol fluorescence method highly cost-effective in practical applications.   Matters needing attention 1. Sample processing: The sample to be tested may contain other metal ions, organic compounds, or impurities, which may react with luminol and interfere with the detection of iron ions. 2. Reaction conditions: Appropriate pH value, temperature, and reaction time are key factors affecting the luminescence effect. Therefore, before conducting the detection, it is necessary to carefully adjust these conditions to achieve better luminescence effects. 3. Reagent storage: Luminol reagents should be stored in a dry, cool, and dark environment to prevent them from becoming ineffective due to moisture, heat, or exposure to light. Luminol has broad application prospects and significant advantages in detecting iron ions in industry. By understanding its detection principle, methods, and precautions, use luminol for the detection and analysis of iron ions. As a manufacturer of chemiluminescent reagents such as Luminol, Desheng can supply analytical pure raw material powders. The solution preparation method is simple, and the sensitivity is high, with stable luminescence. It can assist you in quickly completing experiments in a short period of time. Due to the manufacturer's sale, the price is cheap. If you have any relevant intentions, please feel free to click on the website to inquire about details and purchase at any time!  
Latest company new about Separation gel improves serum yield
2024/06/21

Separation gel improves serum yield

In medical laboratories, the collection and processing of serum is a crucial task. Serum, as an important component of blood, contains a large amount of biological information and has important guiding significance for the diagnosis and treatment of diseases and pains. However, in traditional serum collection methods, there are often problems such as low serum collection rates and cumbersome operations. In recent years, with the continuous development of separation gel technology, it has shown significant advantages in improving serum yield. This article will explore the principle and application of separation gel, as well as its impact on serum yield. The principle of separating adhesive Separation gel is a gel like substance with special physical and chemical properties that can form a stable isolation layer between serum and blood clots under the action of centrifugal force. This isolation layer can effectively separate serum from blood cell components such as red blood cells and white blood cells, thereby increasing the recovery rate of serum. The composition and properties of the separation gel determine its good stability and biocompatibility, without affecting the biochemical characteristics of the serum.   The application of separation gel in serum collection In medical laboratories, separation gel is usually added to blood collection vessels and used together with anticoagulants. When blood collection vessels containing separation gel and anticoagulants are used to collect blood samples, the separation gel rapidly forms an isolation layer between the serum and blood clots under the action of centrifugal force. This isolation layer can effectively block the entry of hemostatic cell components into the serum layer, thereby ensuring the purity and yield of the serum. Meanwhile, the separation gel can also protect the serum from external pollution and oxidation, maintaining its biological activity. The effect of separation gel on serum recovery rate Compared with traditional serum collection methods, separation gel technology can significantly improve the yield of serum. This is mainly due to the following aspects: 1. Good stability: The separation gel can quickly form a stable isolation layer under the action of centrifugal force, preventing blood cell components from entering the serum layer. This characteristic ensures the purity and yield of the serum. 2. Good biocompatibility: The separation gel has good biocompatibility and will not affect the biochemical characteristics of the serum. This enables the separation gel technology to be widely applied in various clinical tests and research. 3. Easy to operate: The blood collection tube using separation gel technology does not require complex pre-treatment operations, only the blood collection tube needs to be placed in a centrifuge for centrifugation. This feature simplifies the serum collection process and improves work efficiency. The advantages of separation adhesive technology In addition to improving serum yield, separation gel technology also has the following advantages: 1. Increased purity and stability of serum: Separation gel technology can prevent blood cell components from entering the serum layer, ensuring the purity and stability of serum. This makes the biological information in serum more accurate and reliable, which is beneficial for the diagnosis and treatment of diseases and pains. 2. Simplified clinical testing process: The use of separation gel technology for blood collection does not require complex preprocessing operations, simplifying the clinical testing process. At the same time, the separation gel can also protect the serum from external pollution and oxidation, prolonging the storage time of the serum. 3. Improved work efficiency: The separation gel technology simplifies the serum collection process and improves work efficiency. This is of great significance for the diagnosis and treatment of emergency patients. In summary, separation gel technology, as an advanced serum collection method, has shown significant advantages in improving serum yield. With the continuous development of medical technology, separation gel technology will play a more important role in clinical testing and research. Hubei Xindesheng Material Technology Co., Ltd. is a professional manufacturer of blood vessel additives. Since its establishment in 2005, it has been engaged in the research and production of separation gels for 19 years with rich experience. And it has its own production plant and laboratory, and the indicators used are strictly formulated by R&D personnel according to experimental requirements. With high daily production and stable performance, it is a priority choice for domestic and foreign customers. If needed, please feel free to contact us.
Latest company new about Why choose EDTA K2 anticoagulant for blood type identification?
2024/06/19

Why choose EDTA K2 anticoagulant for blood type identification?

In the field of hematological testing, blood type identification is a fundamental and crucial test, which has profound significance for blood transfusion, organ transplantation matching, and genetic research. Among the many available anticoagulants, ethylenediaminetetraacetic acid dipotassium salt (EDTA K2) is widely used as the standard anticoagulant for blood type identification, which contains profound scientific considerations and practical advantages. This article will explore why EDTA K2 has become an anticoagulant for blood type identification from multiple dimensions, including its anticoagulant mechanism, impact on blood cell morphology and blood type antigens, ease of operation, comparison with other anticoagulants, and its clinical and scientific applications.   1、 The anticoagulant mechanism of EDTA K2 EDTA K2 belongs to the salt of ethylenediaminetetraacetic acid (EDTA) and is known for its strong chelating ability, especially its ability to effectively bind calcium ions (Ca ²+) in the blood. Calcium ions play a core role in the process of blood coagulation, participating in key steps such as activation of prothrombin and conversion of fibrinogen to fibrin. EDTA K2 forms a stable complex with calcium ions, blocking the calcium dependent coagulation pathway and preventing blood clotting. This feature ensures that the blood sample remains liquid after collection, facilitating subsequent detection and analysis. 2、 Effects on blood cell morphology and blood type antigens Maintaining the natural morphology of blood cells and the integrity of blood type antigens is crucial in blood type identification. EDTA K2 has a relatively small impact on the integrity of the red blood cell membrane while chelating calcium ions, avoiding false positive or false negative results caused by changes in cell morphology. In addition, it does not affect the expression of blood type antigens, ensuring the accuracy of blood type identification. In contrast, certain anticoagulants such as heparin may affect platelet function and interfere with the recognition of blood type antigens, while sodium citrate may alter the volume and morphology of red blood cells, affecting blood type determination. 3、 Convenience of operation and sample stability A significant advantage of EDTA K2 lies in its ease of use. It can be attached to the inner wall of the blood collection vessel through the spray drying technology to ensure that the anticoagulant and blood are quickly and evenly mixed during the blood collection process, without additional operation steps. In addition, EDTA K2 anticoagulant blood samples can maintain stability for a long time under refrigeration conditions, facilitating transportation and storage, which is particularly important for cross regional blood type identification. This stability also allows the laboratory enough time to conduct detailed testing after receiving the sample, without worrying about the timeliness of the blood sample.   4、 Comparison with other anticoagulants Compared with commonly used heparin and sodium citrate, EDTA K2 has a more significant specificity advantage in blood type identification. Although heparin can effectively anticoagulate, it may interfere with the activity of certain enzymes, affect platelet function, and is not suitable for measuring platelet count and enzyme activity. Sodium citrate is mainly used for extracorporeal circulation and emergency anticoagulation. Its anticoagulation mechanism depends on the chelation of calcium ions and alkalization of blood, which may cause red blood cell swelling and affect the accuracy of blood type identification. EDTA K2 is relatively mild and has less interference with blood cells, making it more suitable for blood type identification, which is highly sensitive to cell morphology and antigen expression.   In summary, the reason why EDTA K2 has become the preferred choice for anticoagulants in blood type identification is because its anticoagulant mechanism can effectively prevent blood coagulation without damaging blood cell structure, maintain the true expression of blood type antigens, and demonstrate comprehensive advantages in ease of operation, sample stability, and comparison with other anticoagulants. These characteristics ensure the accuracy and reliability of blood type identification, and play an irreplaceable role in promoting relevant scientific research progress. Desheng stands out in the fiercely competitive market with its advantages and characteristics of high quality, strong research and development capabilities, and customized services. Choosing Desheng as your supplier of ethylenediaminetetraacetic acid dipotassium salt will provide you with high-quality products, professional services, and reliable guarantees. If you have any purchasing intentions, please click on the website to inquire about details!  
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