China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Acridine esters, as an important class of chemiluminescent reagents, are widely used in fields such as immunoassay, nucleic acid detection, and biosensors due to their high sensitivity, rapid luminescence, and low background interference. However, its unique chemical properties require strict adherence to specific specifications during the dissolution process, otherwise it may lead to reagent deactivation or experimental failure. This article will start from the preservation characteristics of acridine esters and systematically analyze their scientific dissolution methods and operational points. Preservation characteristics of acridine ester: necessity of freeze-dried powder and low-temperature light avoidance Acridine esters are typically provided in the form of freeze-dried powder, which is designed to inhibit hydrolysis reactions and prolong reagent stability by removing moisture. The freeze-drying process can remove over 95% of the moisture, keeping the acridine ester molecules in an inactive state while avoiding aggregation or degradation caused by the presence of moisture. In addition, low temperature (usually -20 ℃ or lower) and light avoidance conditions are key to preserving acridine esters: low temperature can slow down molecular thermal motion and reduce hydrolysis rate; Avoiding light can prevent structural damage caused by photosensitive reactions. For example, acridine esters containing NHS (N-hydroxysuccinimide) groups are highly sensitive to both light and moisture, and exposure to room temperature or light for several hours can result in a loss of activity of over 50%. Selection of dissolution medium: necessity of non protonated solvents The dissolution of acridine esters requires avoidance of aqueous solutions, which is based on the unique chemical structure. The ester bonds and NHS groups in acridine ester molecules are highly susceptible to nucleophilic attack reactions with water molecules, leading to hydrolysis cleavage. Especially for acridine esters containing NHS groups, their hydrolysis half-life is only a few minutes to a few hours in water, but can be extended to several days or even weeks in non protonated solvents. Therefore, the following two types of solvents should be used to dissolve acridine esters: 1. Polar non proton solvents: such as dimethyl sulfoxide (DMSO) and N, N-dimethylformamide (DMF), which have no active hydrogen in their molecules and cannot provide protons to participate in hydrolysis reactions. At the same time, they can effectively dissolve the hydrophobic acridine ring structure of acridine esters. DMSO has become the most commonly used choice in laboratories due to its low toxicity, high boiling point (189 ℃), and good biocompatibility; DMF is suitable for the preparation of high concentration acridine esters due to its stronger solubility. 2. Mixed solvent system: For extremely insoluble acridine ester derivatives, a mixed solvent of DMSO and acetonitrile (ACN) or dimethylacetamide (DMA) can be used to promote dissolution by adjusting polarity. For example, mixing DMSO and ACN in a volume ratio of 7:3 can reduce the viscosity of the solution while maintaining its non protonated properties, making it easier for subsequent operations. Adaptation of application scenarios: from labeling reaction to luminescence detection The dissolved acridine ester solution can be directly used for chemiluminescence labeling of proteins, antibodies, or nucleic acids. For example, in immunoassays, acridine ester antibody conjugates can bind to antigens in aqueous buffer and subsequently trigger chemiluminescence reactions by adding hydrogen peroxide and sodium hydroxide. It is worth noting that the labeling reaction requires strict control of pH (usually 7.2-7.6) and ionic strength to avoid affecting the luminescence efficiency of acridine esters. Conclusion The dissolution of acridine ester is a key link connecting its stable storage and efficient application. By selecting non protonated solvents, standardizing operating procedures, and adapting to application scenarios, the chemiluminescence potential of acridine esters can be fully released, providing a highly sensitive and specific solution for biological detection. In the future, with the development of new anti hydrolysis acridine ester derivatives, their dissolution and application conditions are expected to be further optimized, promoting breakthroughs in chemiluminescence technology in more fields. As a manufacturer of luminescent reagents, Desheng is currently fully committed to supplying a range of high-quality acridine ester powders. These products are not only convenient to use, but also known for their luminescence sensitivity, ensuring that you can obtain accurate and reliable experimental results in a very short period of time. If you have purchasing needs or would like to learn more details, please feel free to click on our official website for consultation.  

Free Fatty Acids (FFA), as the core intermediate product of human energy metabolism, its abnormal level is closely related to diabetes, obesity, cardiovascular disease and metabolic syndrome. Accurately and rapidly measuring FFA concentration is not only a key indicator for clinical diagnosis, but also an important tool for drug development and nutritional assessment. Among numerous detection methods, the colorimetric substrate TOPS enzyme photometric method has gradually become the mainstream technology for FFA determination due to its high sensitivity, strong stability, and convenient operation. Technical principle: Perfect combination of enzymatic reaction and colorimetric reaction The core of TOPS enzymatic photometry is to link the enzymatic oxidation process of FFA with colorimetric reaction, and quantitatively analyze it through colorimetric method. Specifically, the detection process is divided into two steps: 1. Enzymatic oxidation stage: FFA in the sample is catalyzed by acetyl CoA synthase to bind with ATP and CoA to generate acetyl CoA, while releasing pyrophosphate; Subsequently, acetyl CoA oxidase further oxidizes acetyl CoA to produce hydrogen peroxide (H ₂ O ₂). 2. Color reaction stage: Under the catalysis of peroxidase (HRP), H ₂ O ₂ undergoes redox reaction with the color substrate TOPS and 4-aminoantipyrine (4-AAP) to generate stable red quinone imine compounds. The color depth of the compound is linearly related to the concentration of FFA, and the FFA content can be accurately calculated by measuring the absorbance with a spectrophotometer (usually at a wavelength of 550-570nm). Technical advantages: sensitive, stable, easy to operate Compared with traditional methods, TOPS enzyme photometry exhibits significant advantages: 1. High sensitivity: TOPS has a much higher molar absorptivity than traditional color reagents (such as copper reagents), and can detect FFA as low as μ mol/L. It is particularly suitable for precise analysis of low concentration samples such as serum. 2. Strong stability: TOPS has stable chemical properties and is not easily affected by temperature, pH fluctuations, or sample matrix interference, ensuring the repeatability and reliability of detection results. In addition, the kit format (dual reagent system) further simplifies the operation process and reduces human errors. 3. Convenient operation: No complex preprocessing steps (such as organic extraction or derivatization) are required. The sample can be directly mixed and incubated with the reagent, and the detection can be completed in 10-15 minutes, greatly reducing the detection time. 4. High cost-effectiveness: Compared with gas chromatography or liquid chromatography, TOPS enzyme photometry does not require expensive instruments and equipment, has low reagent consumption, and is suitable for large-scale promotion in clinical laboratories. Clinical Application: From Disease Diagnosis to Health Management The application scenarios of TOPS enzyme photometry are extensive, covering multiple fields such as clinical diagnosis, metabolic research, and drug development 1. Cardiovascular disease risk assessment: The increase of FFA level is an independent risk factor for atherosclerosis and myocardial ischemia. Regular detection can assist in early diagnosis and prognosis monitoring. 2. Metabolic disease management: diabetes patients are often accompanied by FFA metabolic disorder. Dynamic monitoring of FFA concentration can optimize insulin treatment programs and control blood sugar fluctuations. Conclusion: The TOPS enzyme photometry method, based on innovative scientific principles and practical technical implementation, provides an efficient and accurate solution for FFA detection. From clinical diagnosis to health management, from basic research to drug development, this technology is continuously driving the progress of metabolic medicine and safeguarding human health. The TOPS and other the new Trinder's reagents produced by Hubei Xindesheng Material Technology Co., Ltd. have high purity, good water solubility, stable production process, and small inter batch differences. All indicators meet relevant standards. If you have any purchasing needs in the near future, please feel free to contact me or click on the official website for more details!  

In biological and biochemical experiments, biological buffering agents serve as the core reagent for maintaining solution pH stability, and their sterilization treatment directly affects the accuracy and reliability of experimental results. From cell culture to protein purification, from nucleic acid research to drug development, the sterilization decision of buffer solution needs to be comprehensively judged based on experimental type, reagent characteristics, and operating standards. The necessity of sterilization: the type of experiment determines the risk level 1. Rigid requirements for high-sensitivity experiments In experiments such as cell culture, gene editing, or single-molecule testing that require high levels of sterility, microbial contamination may lead to cell death, abnormal gene expression, or signal interference. For example, although MOPS buffer is commonly used for RNA electrophoresis, its pH stability is easily affected by microbial metabolites. If the experiment involves rare samples or long-term cultivation, high-pressure sterilization can effectively eliminate potential sources of contamination. Similarly, after high-pressure sterilization, the pH stability of BES buffer solution is significantly improved, making it suitable for cell experiments sensitive to ion strength. 2. Stability guarantee for long-term storage Unterilized buffer solutions may breed microorganisms during storage, leading to pH drift or precipitation. For example, phosphate containing buffer solutions are prone to form insoluble complexes with calcium and magnesium ions at high temperatures, and sterilization treatment can delay this process. For Tris HCl buffer solutions that require long-term storage, high-temperature and high-pressure sterilization can ensure their chemical stability and avoid degradation of active ingredients caused by microbial growth. 3. Avoid external protein interference The proteins contained in microorganisms themselves may interfere with experimental results through cross reactions. In Western Blot experiments, if the buffer is not sterilized, bacterial proteins may undergo non-specific binding with the target protein, leading to false positive signals. Sterilization treatment can completely remove such interference sources, especially suitable for low abundance protein detection or high specificity antibody incubation scenarios. Practical considerations for sterilization: balancing cost and benefit 1. Experimental cost and operational complexity High pressure sterilization requires specialized equipment and takes a long time, while membrane filtration, although fast, requires regular replacement of the membrane. For large-scale experiments, membrane filtration may increase the cost of consumables; For small-scale or high-value experiments, the benefits of sterile assurance far exceed the costs. For example, in the production of gene therapy vectors, the use of sterilization buffer can avoid batch contamination risks and ensure product safety. 2. Supplementary role of operating standards Even if the buffer has been sterilized, sterile operating procedures must still be followed throughout the experiment. For example, using sterile pipette tips during the transfer process and operating in ultra clean workbenches can further reduce the risk of contamination. In addition, experimenters wearing lab coats, gloves, and goggles can avoid secondary contamination of the buffer solution by human microorganisms. Conclusion: Scientific decision-making and precise sterilization The sterilization treatment of biological buffering agents is not a one size fits all approach, but requires comprehensive decision-making based on the type of experiment, reagent characteristics, and operating conditions. Sterilization is a necessary means to ensure the reliability of results for high-sensitivity experiments, long-term storage, or scenarios involving external protein interference; For routine experiments or thermosensitive buffering agents, membrane filtration or strict aseptic operation can replace high-pressure sterilization. By scientifically selecting sterilization methods and standardizing operating procedures, the performance of biological buffering agents can be maximized, providing solid support for biological research. Hubei Xindesheng Material Technology Co., Ltd. is a manufacturer of diagnostic reagent raw materials, which can provide various biological buffering agents, including Tris, Tris HCl, Bis Tris, Bicine, TAPS and other reagents. If you need to purchase, please feel free to contact us at any time!  

On the morning of September 3, 2025, Hubei Xindesheng Material Technology Co., Ltd. organized all employees to watch the live broadcast of the grand September 3 military parade, witnessing the magnificent strength of the country and feeling the spirit of the times together. The activity aims to deepen patriotic education, enhance team cohesion, and inspire employees' sense of national pride and professional mission.   At 9 o'clock in the morning, the company conference room was filled with a solemn and enthusiastic atmosphere. All employees hold bright red flags issued by the company and watch the parade on the big screen with focused expressions. When the imposing troops marched with resolute steps, when advanced weapons and equipment appeared one by one, and when the fighter jets soaring in the blue sky traced magnificent paths, sincere admiration and warm applause erupted from the scene time and time again. The employees waved the red flag in their hands, sincerely feeling proud and honored for the increasingly strong comprehensive national strength and glorious achievements in national defense construction of the motherland.   Chairman Wang Zhongxi passionately elaborated on the profound enlightenment brought by watching the military parade in his speech. He first mentioned emotionally that the resolute figure of all the soldiers who stood still for hours in the scorching heat is a reflection of the precious craftsmanship spirit of this era. Behind this perseverance is the noble choice of integrating personal values into the destiny of the country. The people of Xindesheng should also cherish their country and closely link their personal growth with the development of enterprises and the country. Mr. Wang also pointed out that the grand military parade demonstrated the prosperity and stability of the country, which is the solid foundation for enterprises to operate with peace of mind and continue to develop. New Desheng must integrate its own development into the overall development of the country and repay the opportunities given by the times with practical actions.   Chairman Wang Zhongxi called on all employees of Xindesheng to transform the surging enthusiasm and deep patriotism inspired by watching the military parade into practical actions that are down-to-earth. He demands that everyone deeply study and understand the spirit of the military parade, which embodies perseverance, loyalty, collaboration, and responsibility, and inject it into every research and development project, every production process, and every market service. With a more vigorous fighting spirit and an attitude of striving for excellence, we are committed to innovation and breakthroughs in the field of IVD. With outstanding work performance and continuously rising corporate achievements, we contribute our wisdom and strength to the prosperity of the local economy and the technological progress of the industry. We turn this ambitious mission of serving the country into a sincere and moving gift to our great motherland.   Hubei Xindesheng is currently in a new stage of rapid development. The construction of a new production base in Huanggang has entered an accelerated stage, aiming to create a production base for high-end biological buffering agents in China, in order to break through import dependence and achieve domestic substitution of key raw materials. We firmly believe that Hubei Xindesheng will transform the pride and sense of mission inspired by the military parade into an inexhaustible driving force for promoting the independent and controllable development of the IVD industry chain with high quality. With higher quality innovative products and outstanding industry contributions, we will serve our ancestors  

In recent years, the in vitro diagnostic (IVD) industry in China has encountered unprecedented development opportunities driven by multiple factors such as the continuous growth of global medical demand, technological progress, and population aging. By 2025, the size of China's IVD market is expected to exceed 135 billion yuan, becoming an indispensable and important component of the modern healthcare system. Industry status: Steady growth, diverse highlights in segmented fields The IVD industry in China has shown a steady growth trend in recent years. According to market research institutions, the size of China's IVD market has reached 125 billion yuan in 2024, a year-on-year increase of 8.5%. It is expected that by the end of 2025, the market size will exceed 135 billion yuan, with a growth rate maintained at around 8%. In the segmented field, immunodiagnosis still holds the largest market share, thanks to the continuous development of technologies such as chemiluminescence and electrochemiluminescence. Its applications in infectious diseases, tumor markers, thyroid function and other detection projects are becoming increasingly widespread. Molecular diagnostics, as the fastest growing sub field, has an average annual growth rate of about 20%. The continuous innovation of technologies such as PCR, NGS, dPCR, etc. has promoted their widespread application in infectious disease diagnosis, tumor gene testing, and other fields. The POCT market, with its fast and convenient characteristics, has a strong demand in primary healthcare, emergency treatment and other scenarios, and is expected to maintain a high growth rate in the coming years. Technological innovation: the core driving force for industry development Technological innovation is a key factor driving the development of the IVD industry. In the field of immunodiagnosis, the integration of chemiluminescence technology with microfluidics and AI has become an important trend, greatly improving the sensitivity and automation level of detection. In the field of molecular diagnostics, the continuous optimization and upgrading of PCR technology, especially the application of digital PCR (dPCR), has further improved the accuracy of detection, providing strong support for tumor liquid biopsy, rare disease gene detection, and so on. POCT technology is developing towards miniaturization, intelligence, and multi index joint inspection. By combining IoT and AI technology, it achieves real-time transmission and intelligent analysis of detection data, meeting the needs of primary healthcare, home self inspection, and other scenarios. Competitive landscape: Market reshuffle under diversified situation The competitive landscape of China's IVD industry is showing a diversified trend, with foreign-funded enterprises and local enterprises competing on the same stage. Foreign funded enterprises dominate the high-end market with advanced technology and brand advantages, but with the continuous breakthroughs in local enterprise technology and the improvement of product quality, their market share is gradually being squeezed. Local enterprises, relying on cost advantages, in-depth understanding of the local market, and policy support, occupy a large share in the mid to low end market and continuously penetrate into the high-end market. The implementation of centralized procurement policy and the promotion of medical insurance payment reform have accelerated market reshuffle, putting greater pressure on small and medium-sized enterprises to survive, and further concentrating market share towards top enterprises. Future trend: Emerging markets and international development become new growth points Looking ahead, the IVD industry in China will continue to maintain a stable growth trend. With the continuous growth of medical demand, the promotion of technological innovation, and the optimization of policy environment, the industry scale will continue to expand. Emerging markets such as pet medical testing and consumer health testing will become new growth points, bringing new development opportunities to the IVD industry. As an upstream IVD reagent raw material manufacturer, Hubei Xindesheng Company can supply a variety of raw materials suitable for molecular diagnostics and other fields, including biological buffering agents, luminescent reagents, enzyme reaction substrates, etc. Hubei Xindesheng Material Technology Co., Ltd. has always maintained the research and development production philosophy of "exploring and innovating, pursuing excellence" in the face of current challenges and opportunities. At the same time, it actively expands overseas markets, participates in international competition, and enhances its international market share.    

In biochemical experiments, CAPS buffer, as an important alkaline buffer, is widely used in Western blotting, enzyme catalyzed reactions, and HPLC separation due to its stable pKa value (about 10.4), good water solubility (up to 11.07 mg/mL at 25 ℃), and low cell membrane permeability. However, experimenters often find that the solubility of CAPS significantly decreases at low temperatures (such as 4 ℃ or -20 ℃), leading to difficulties in buffer preparation or uneven concentration, which in turn affects the reliability of experimental results. This article will analyze this phenomenon from three levels: molecular mechanisms, environmental factors, and experimental operations, and propose targeted optimization solutions. Molecular mechanism of low temperature solubility decrease The dissolution process of CAPS is essentially the process of its molecules forming a hydration layer with water molecules through hydrogen bonding. At room temperature, the sulfonic acid group (- SO3H) and amino group (- NH -) in CAPS molecules combine with water molecules through polar interactions to form stable solute solvent complexes. However, as the temperature decreases, the thermal motion of water molecules weakens, and the hydrogen bonding network tends to become rigid, resulting in a decrease in the binding energy between CAPS molecules and water molecules. Experimental data shows that the solubility of CAPS decreases by about 30% at 4 ℃ compared to 25 ℃, which is closely related to changes in the dynamic properties of water molecules. In addition, the crystallization behavior of CAPS undergoes significant changes at low temperatures. At room temperature, CAPS molecules are dispersed in a disordered state in solution; When the temperature drops below the critical point, molecules form an ordered lattice structure through hydrophobic interactions and π - π stacking. This phase transition process will further reduce the solubility of CAPS and even lead to the precipitation of undissolved solid particles. For example, when preparing CAPS buffer solution, if the solvent is not sufficiently preheated, white flocculent precipitates can often be observed, which is a direct manifestation of low-temperature induced crystallization. The synergistic effect of environmental factors on solubility In solvent ion strength experiments, deionized water is often used to prepare CAPS buffer solution. However, if there are residual metal ions (such as Ca ² ⁺, Mg ² ⁺) in the water, they will form complexes with the sulfonic acid groups in CAPS molecules, reducing their effective solubility. At low temperatures, the hydration of ions is enhanced, the stability of the complex is improved, and further exacerbating the decrease in solubility. For example, in a solution containing 0.1 mM Ca ² ⁺, the solubility of CAPS at 4 ℃ is reduced by 15% compared to pure water system. The solubility of CAPS is closely related to its dissociation state due to pH fluctuations. When the pH is below pKa (10.4), CAPS molecules exist in protonated form (- SO3H) with high solubility; When the pH approaches or exceeds pKa, the solubility of the deprotonated form (- SO ∝⁻) significantly decreases. Under low temperature conditions, the pH value of the buffer solution may drift due to the dissolution of CO ₂ or hydrolysis of impurities, indirectly affecting the dissolution behavior of CAPS. By understanding the molecular mechanism and environmental factors that contribute to the decrease in low-temperature solubility of CAPS, researchers can optimize the preparation process and storage conditions in a targeted manner to ensure the stability of the buffer performance. In the future, with the development of new zwitterionic buffering agents, the limitations of CAPS in low-temperature experiments are expected to be fundamentally resolved. As a professional biological buffering agent manufacturer, Desheng is committed to providing high-quality CAPS buffering agents. We not only have professional personnel to supervise and control the process from raw material use to factory preparation, but also continuously optimize testing methods to meet the diverse needs of our customers. There is stock available for sale in the warehouse at a cheap price. If you have any relevant intentions, please feel free to click on the website to inquire about details and purchase at any time!

In cell culture and biological experiments, HEPES buffer and phenol red indicator are often used simultaneously. The former is used to maintain pH stability in the culture medium, while the latter is used to visually display pH changes. However, the interaction between the two in terms of chemical properties may lead to color abnormalities, affecting the accuracy of experimental results. This article will analyze the causes of this phenomenon and provide simplified optimization solutions. Chemical basis of color interference Phenol red is a pH sensitive dye that changes color with acidity or alkalinity: it appears yellow in acidic environments (pH8.2). This characteristic makes it an ideal tool for monitoring the pH of cell culture media. The core function of HEPES as a buffer is to stabilize pH by releasing or absorbing hydrogen ions. The problem is that the sulfonic acid groups in HEPES molecules have a similar chemical structure to phenol red, and when the concentration of HEPES is high, they will interact with phenol red to change its molecular structure. This change causes the color of phenol red to become lighter or shift at a specific pH, for example, it may appear orange red instead of standard red at pH 7.4. The intuitive manifestation of interference phenomenon In conventional cell culture, if high concentrations of HEPES (such as over 20mmol/L) and phenol red are used simultaneously, the color of the culture medium may be lighter or yellowish than expected. For example, the pH 7.4 culture medium, which should have displayed red, may have turned pale orange due to HEPES interference, leading researchers to misjudge the pH value. In fluorescence microscopy observation, this interference is more pronounced. Phenol red emits fluorescence at specific wavelengths, while HEPES may absorb some of the fluorescence signal, resulting in decreased image brightness or increased background noise. This effect is particularly prominent in long-term observation or live cell imaging experiments, which may mask the true state of cells. Simplified optimization plan Adjust HEPES concentration 1. Conventional cultivation: Control the concentration of HEPES within 10-15 mmol/L, which has minimal interference with the color development of phenol red and can effectively maintain pH stability. 2. Short term experiment: If the experimental time is short (such as

In the use of the new Trinder's reagent TODB, high background is a common problem that affects the detection results, while insufficient sealing and incomplete plate washing are key factors that are easily overlooked during operation. These two issues can cause an abnormal increase in the color background due to non-specific binding and interference from residual substances. Below is a detailed analysis and solution. Inadequate closure: the 'behind the scenes' driving force behind non-specific combinations The function of the blocking step is to block the non binding sites on the surface of the reaction carrier (such as an enzyme-linked immunosorbent assay plate). If the sealing is not sufficient, the substrates, enzyme conjugates, and other components in the TODB reagent will randomly adsorb onto the surface of the carrier, forming a color signal without the participation of the target substance, directly increasing the background value. specific reason 1. Insufficient sealing time: Generally speaking, sealing requires placing at 37 ℃ for 60 minutes or at room temperature for 120 minutes. If the time is shortened to less than 30 minutes, the active sites on the surface of the carrier cannot be completely covered by the blocking solution (such as BSA, skim milk powder), and those hydrophobic areas that are not blocked will actively adsorb protein components in the TODB reaction system, resulting in background coloration. 2. Too low concentration of blocking solution: When the effective ingredients in the blocking solution are insufficient, such as when the original 5% BSA drops to 1%, a tight protective film cannot be formed between molecules. Components such as horseradish peroxidase in TODB reagent will stick to the inner wall of the plate pore through hydrophobic interactions, and non-specific reactions will occur with the substrate during the reaction. 3. Failure of sealing solution: If the sealing solution is repeatedly frozen and melted, or stored for more than its expiration date, the protein components inside will deteriorate and lose their sealing function, resulting in many "exposed" sites on the surface of the carrier, which become the source of background signals. Incomplete board washing: the "stacking effect" of residual substances The main function of plate washing is to remove unbound free reagents (such as unbound antibodies, TODB precursor substances). If the plate washing is not thorough, residual substances will continue to participate in color development in subsequent reactions, allowing background interference to accumulate. Specific Reason 1. Too few plate washes: Conventional testing requires washing the plate 3-5 times. If it is reduced to less than 2 times, the residual free enzyme complexes in the well cannot be completely removed, and the reaction will react with the TODB substrate, resulting in additional color development. 2. Too much detergent residue: After washing the board, if the holes are not inverted on the absorbent paper and patted dry, there will be more detergent residue in each hole, and some components inside will disrupt the balance of the TODB reaction system. At the same time, residual enzyme conjugates will diffuse into the adjacent holes with the liquid, causing cross contamination and raising the background. 3. There is a problem with the washing machine: When the needle of the washing machine is blocked or the pressure is insufficient, the corners of the plate holes will become areas that cannot be washed. The residual TODB reagent will crystallize after drying and participate in the reaction when dissolved again, causing the local background color to darken. Summary: Operational details determine the effectiveness of background control Although sealing and washing plates are routine steps, their quality directly affects the background level of TODB reagents. In practical use, it is recommended to adopt the method of "extending the sealing time+increasing the number of plate washes", combined with measures such as checking the concentration of the sealing solution and regularly calibrating the washing machine, which can significantly reduce the background value and provide assurance for the accuracy of the detection results. Desheng specializes in producing more than ten new Trinder's reagents, including TODB. After more than ten years of research and development, it can ensure that TODB appears as a powder with a purity of up to 99.5%, strong water solubility, and stable performance to ensure the accuracy of experimental results. Desheng has a place in the market for in vitro diagnostic kit raw materials with high-quality products, and is deeply trusted and supported by customers at home and abroad. If you have any relevant intentions, please click on the official website for consultation!  

In the field of biochemical detection, the selection of colorants plays a crucial role in the sensitivity, accuracy, and stability of detection methods. At present, there are various types of colorants in the market, such as TMB widely used in enzyme-linked immunosorbent assay (ELISA), which has become a common choice for many detection scenarios due to its wide applicability. However, in the detection of specific indicators, the new Trinder's reagent has demonstrated unique and excellent performance, with TOPS reagent showing significant advantages in the determination of fatty acids. Outstanding advantages in sensitivity and accuracy In biological detection, sensitivity and accuracy are the core indicators for measuring the quality of detection methods. When using TOPS reagent to determine serum free fatty acids (FFA), its sensitivity is extremely excellent. The content of serum free fatty acids in the body is relatively low, and even small changes in their concentration may be closely related to various physiological and pathological states. TOPS reagents can accurately capture these subtle concentration changes, and even low concentrations of serum free fatty acids can be effectively detected, providing more accurate data support for clinical diagnosis and research. Meanwhile, TOPS reagents are equally accurate. During the detection process, it can minimize the influence of interfering factors and ensure that the detection results truly and reliably reflect the content of free fatty acids in the serum. Compared with some traditional colorants, the detection results of TOPS reagent have higher repeatability and consistency, effectively avoiding errors caused by unstable properties of colorants, and providing a solid basis for scientific research and clinical decision-making. Easy and convenient operation In addition to high sensitivity and accuracy, the ease of operation is also a major highlight of TOPS reagents. In practical testing, the method of using TOPS as a chromogenic substrate to detect serum or plasma FFA is relatively simple in steps, without the need for complex instrument equipment and cumbersome operating procedures. Researchers or clinical laboratory personnel only need to follow the standard experimental operation guidelines, mix the sample with TOPS reagents and related reagents, and after an appropriate reaction time, detect it using conventional instruments such as spectrophotometers. This simple and convenient operation method not only improves detection efficiency and reduces experimental costs, but also reduces errors caused by operational errors, making the detection results more reliable. Excellent performance in multiple standards Among numerous Trinder's colorants, TOPS reagent stands out on multiple key performance indicators. Secondly, TOPS reagents have high stability. During storage and use, it is not easily decomposed or deteriorated, and can maintain its chemical stability for a long time, providing reliable assurance for detection. In addition, the molar absorptivity of TOPS reagent is high, which means that it has strong light absorption ability and can produce significant color changes at lower concentrations, thereby improving the sensitivity and detection limit of the detection. In summary, the new Trinder's reagent TOPS has significant advantages in determining fatty acids, such as high sensitivity, good accuracy, simple and convenient operation, and excellent performance indicators. With the continuous development of biological detection technology, TOPS reagents are expected to play a more important role in the field of fatty acid detection, providing stronger support for life science research and clinical diagnosis. Desheng specializes in producing more than the new Trinder's reagents, including TOPS. After more than ten years of research and development, it can ensure that TOPS appears as a powder with a purity of up to 99.5%, strong water solubility, and stable performance to ensure the accuracy of experimental results. Desheng has a place in the market for in vitro diagnostic kit raw materials with high-quality products, and is deeply trusted and supported by customers at home and abroad. If you have any relevant intentions, please click on the official website for consultation!  

On August 19, 2025, a group of five senior leaders including Wang Zhongxi, Chairman of Hubei Xindesheng Material Technology Co., Ltd., and Wang Anqi, General Manager, arrived at the construction site early in the morning to conduct on-site inspections of the project progress and provide important guidance for the project construction. This marks the first important milestone in the construction of the new factory since the groundbreaking ceremony on July 18th. Since the groundbreaking ceremony on July 18th, significant progress has been made in the construction of the Huanggang new factory in just one month. The once flat land has now begun to take shape, and various tasks such as factory foundation construction, steel structure construction, and equipment entry are being carried out intensively. The machines on the construction site roared, and the workers raced against time, creating a lively scene. During the inspection, Mr. Wang emphasized, "The construction speed from the foundation to the present fully demonstrates the efficiency of 'New Desheng'. We must continue to maintain this momentum and ensure the early completion of the new factory. Global strategy, deep layout in the field of high-end biological buffering agents The construction of the Huanggang new factory is not only an important measure for the expansion of Xindesheng's production capacity, but also a key layout for the company to move towards the high-end biological buffer field. With the rapid development of the IVD (in vitro diagnostics) industry, the demand for high-quality and highly stable buffering agents from various types of customers in the global market is increasing day by day. Based on years of technological accumulation and market insights, Xindesheng has positioned Huanggang New Factory as a domestic high-end production base for biological buffering agents, committed to breaking the dependence on imports and promoting the localization and substitution of key raw materials. This high-level inspection marks a new critical stage in the construction of Huanggang's new factory. From laying the foundation to rapid progress, Hubei Xindesheng has once again demonstrated efficient execution and strategic determination. In the future, with the completion and operation of the new factory, Hubei Xindesheng will further consolidate its leading position in the field of IVD raw materials, provide better products and services for the industry, and help the independent, controllable and high-quality development of China's IVD industry chain. Twenty years of accumulation and development, forging a glorious journey Since the establishment of Wuhan Desheng Biochemical Technology Co., Ltd. in April 2005, Hubei Xindesheng has embarked on a journey of professional cultivation. In November 2012, the company obtained its first national patent, laying a solid foundation for technology and quality. In December 2015, the company's sales exceeded 10 million yuan for the first time, and its market expansion took a new step. In December 2020, the company was awarded the title of "High tech Enterprise" and formed strategic alliances with well-known universities such as Hubei University, deeply integrating industry, academia, and research, injecting strong impetus into continuous innovation. These milestones record the company's glorious journey from technological accumulation to market recognition. Capacity leap, industrial upgrading, promising future Looking back at this important milestone, Hubei Xindesheng is advancing industrial upgrading at an astonishing speed. It is reported that the new factory will be equipped with various advanced equipment and is expected to be completed and put into operation in January 2026. At that time, Xindesheng will not only achieve a leapfrog growth in production capacity, but also further consolidate its leading position in the field of diagnostic reagent raw materials with its technological advantages, and contribute greater strength to the independent and controllable development of China's IVD industry chain. Thick accumulation leads to steady progress, and the journey is approaching. Every brick and tile of Huanggang New Factory is writing a brand new chapter for Xindesheng. Hubei Xindesheng is steadily moving towards the goal of becoming a leader in high-end biological buffering agents with a high spirited attitude. From blueprint to reality, from foundation to rise, the people of Xindesheng are interpreting the corporate spirit of "quality first" through practical work. With the rapid progress of the construction of Huanggang New Factory, let us look forward to the early completion of Huanggang New Factory and witness another brilliant leap of Xindesheng!  

In biochemical experiments, biological buffering agents play a crucial role in maintaining the pH stability of solutions and providing a suitable reaction environment for enzymes and other biomolecules. Normally, the use conditions of biological buffering agents are set at room temperature of 25 degrees Celsius, or a higher temperature is selected based on the optimal pH range of the enzyme. However, the diversity of scientific research requires some experiments to be conducted under low-temperature conditions, which poses a serious challenge to the performance of buffering agents in low-temperature environments. HEPES buffer, with its unique properties, has become an ideal choice for enzyme experiments under low-temperature conditions. In biological experiments, temperature is a key influencing factor. Most biological buffering agents are designed with a focus on their effectiveness at room temperature or relatively high temperatures. Under these temperature conditions, they can effectively stabilize the pH value of the solution, ensuring the normal functioning of enzymes and other biomolecules. But when experiments need to be conducted in low-temperature environments, the performance of many buffering agents will be significantly affected. Low temperature may cause changes in the ionization state of these buffering agents, thereby affecting their ability to adjust pH values, resulting in large fluctuations in the pH value of the solution, which is not conducive to the stability and activity maintenance of enzymes. HEPES (4-hydroxyethylpiperazine ethanesulfonic acid) exhibits unique characteristics. Generally speaking, the decomposition ability of buffering agents is closely related to temperature. The decomposition ability of most buffering agents increases with increasing temperature and decreases with decreasing temperature. HEPES is no exception, and its decomposition ability also follows this rule. However, compared to other buffering agents, HEPES has a significant advantage in that its decomposition constant varies less with temperature. This characteristic enables HEPES buffer to maintain relatively stable performance under low temperature conditions. In low-temperature environments, enzyme activity is often inhibited, and its structure and function are more susceptible to changes caused by external factors. HEPES buffer can provide a stable pH environment for enzymes, reducing damage caused by pH fluctuations. It can effectively maintain the charge distribution on the surface of enzyme molecules, maintain the correct conformation of the enzyme, and ensure that the enzyme can still maintain its structural and functional integrity at low temperatures. For example, in some experiments that require low-temperature preservation of biological samples and enzyme activity detection, using HEPES buffer can avoid experimental errors caused by the decrease in buffer performance due to temperature reduction. Researchers can more accurately measure the activity changes of enzymes at low temperatures and further explore the influence of temperature on enzyme reaction kinetics. In addition, in the study of enzyme catalyzed reactions under low temperature conditions, HEPES buffer can also provide stable pH conditions for the reaction system, promote the smooth progress of the reaction, and improve the reliability and repeatability of experimental results. In summary, although most biological buffers have limited performance under low temperature conditions, HEPES buffer has become a reliable buffer for enzyme experiments under low temperature conditions due to its unique advantage of small temperature dependent decomposition constants. It provides strong support for researchers to conduct biochemical research in low-temperature environments, helping to promote our in-depth understanding and exploration of enzymes and other biomolecules. Hubei Xindesheng Material Technology specializes in the production of HEPES and other biological buffering agents. The products have high purity, good buffering capacity, and affordable prices, providing product support for related experiments. If you are also interested in our products, please feel free to contact me!  

Luminol, as a classic chemiluminescence reagent, is widely used in fields such as forensic medicine and biological detection, but its luminescence efficiency is often constrained by multiple factors. This article analyzes the core reasons for its low efficiency from four aspects: reagent preservation, reaction system, experimental operation, and environmental interference. 1, Improper storage of reagents: oxidation and purity degradation Luminol is highly sensitive to light and oxygen. If not sealed in a brown opaque bottle, light will trigger a photochemical reaction and damage the molecular structure; Long term exposure to air can oxidize and produce by-products such as carbonyl compounds. These impurities competitively consume reactive oxygen species (such as hydroxyl radicals) in the reaction system, reducing luminescence efficiency. For example, copper ion (Cu ² ⁺) impurities can form complexes with luminol, hindering its contact with hydrogen peroxide; Residual organic solvents, such as dimethylformamide, may inhibit peroxidase (POD) activity. 2, Imbalance of reaction system: dual regulation of catalyst and acidity/alkalinity Luminol luminescence relies on the process of its oxidation to form 3-aminophthalates, which requires a synergistic effect of catalyst and oxidant. If the concentration or type of catalyst is not appropriate, it can directly lead to an imbalance in reaction rate. For example, the optimal pH for POD is 7.0-8.0, while luminol luminescence requires alkaline conditions (pH 10-12). Excessive sodium hydroxide (NaOH) can damage the POD structure and render it inactive; Insufficient alkalinity prevents the activation of the hydrazide group of luminol, hindering the oxidation reaction. The concentration control of non enzyme catalysts (such as potassium ferrocyanide) is also crucial. When the concentration of iron ions (Fe ³ ⁺) is too high, it will trigger an "instant flash" of luminol, and the reactants will be completely consumed in a very short time, making it impossible to continuously detect the luminescent signal. The data shows that when the concentration of Fe ³ ⁺ exceeds 0.1 mmol/L, the luminescence half-life of luminol is shortened from 120 seconds to less than 5 seconds, significantly reducing the reliability of signal acquisition. 3, Experimental operation error: details determine success or failure The standardization of experimental operations directly affects the luminescence efficiency of luminol. Pipette error is a common problem: an uncalibrated pipette may cause the concentration of luminol to deviate from the theoretical value by more than 20%, thereby affecting the luminescence intensity. Incorrect order of reagent addition can also cause abnormal reactions, such as adding hydrogen peroxide (H ₂ O ₂) first and then dissolving luminol, which can lead to excessive local H ₂ O ₂ concentration and rapid decomposition of luminol into non luminescent products. Uneven stirring is particularly prominent in small volume reaction systems, such as microfluidic chips. If the stirring speed is insufficient or the time is too short, the contact between luminol and oxidant is not sufficient, forming a concentration gradient, causing the luminescent signal to exhibit a distribution characteristic of "center bright, edge dark", reducing the overall detection sensitivity. 4, Environmental interference: invisible killers of light and oxygen The influence of environmental factors on the luminescence of luminol is often underestimated. Strong background light (such as laboratory fluorescent lamps) can excite the fluorescent background of luminol, masking weak chemiluminescence signals. Research has shown that under 500 lux lighting conditions, the signal-to-noise ratio (SNR) of luminol decreases by 60% compared to dark environments, resulting in ineffective detection of low concentration samples (such as 10 ⁻⁹ mol/L). Excessive oxygen content is also detrimental. Although the oxidation of luminol requires oxygen, excessive oxygen can accelerate side reactions (such as hydrogen peroxide dismutation) and reduce the generation of reactive oxygen species. High humidity environments may cause luminol powder to absorb moisture and clump, reducing solubility and reactivity. Experiments have found that when the relative humidity is greater than 80%, the luminescence intensity of luminol can lose up to 40% within 24 hours. As a manufacturer of chemiluminescence reagents such as luminol, Desheng can supply high-purity raw material powders. This high-purity luminol powder not only ensures the accuracy of experimental results, but also improves the sensitivity and stability of luminescence. At the same time, the company is committed to providing customers with high-quality products and services to meet the growing demands of scientific research and the market. If you have any recent purchasing needs, please click on the website to inquire about details and make a purchase!