China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Fluttering banners, embarking on a new journey, fighting fiercely for a hundred days to see the true chapter In the summer of 2025, on the hot land of innovation and entrepreneurship in Huanggang, Hubei, a modern chemical plant representing a new height in the industry is rising at an astonishing speed. Since the foundation laying and departure on July 18th, the Huanggang new factory project of Hubei Xindesheng Material Technology Co., Ltd. has embarked on a construction journey of racing against time with the spirit of "time waits for no one, seize the day and night". In just two months, the project team has written an impressive report card with wisdom and sweat, perfectly interpreting the profound connotation of "Desheng efficiency" with tangible construction results, injecting a shot in the arm for local economic development. Warehouse sealing: Warehouse center takes shape first On September 14, 2025, the project construction once again reached a milestone moment - the main structure of Warehouse 3 successfully completed the topping out work! This advanced design and fully functional storage facility has completed the main construction first, which means that the construction of the new factory has achieved significant results in this stage. The topping out of the warehouse not only creates conditions for subsequent internal decoration and equipment installation, but also ensures the efficient operation of the future factory logistics and warehousing system. At the topping out ceremony, the project leader stated, "The rapid topping out of Warehouse 3 is a testament to our careful organization and scientific construction, and also a good start for the on-time delivery of the entire project. Multi line joint promotion: Comprehensive construction momentum is unstoppable At the same time, the construction of other key nodes in the new factory area is also progressing efficiently and synchronously, presenting a busy scene of multiple blooming points and simultaneous progress. The construction of the gutter and roof system in the 1 # production plant is in full swing, creating favorable conditions for the installation of internal equipment in the plant; The circulating water tank supporting the factory area has completed the base treatment and entered the plastering construction stage, and the bottom plate formwork construction of the base water tank and the initial rainwater tank has also been fully carried out. The synchronous construction of these supporting facilities ensures the coordinated operation of production and environmental protection systems in the future factory area, reflecting New Desheng's high emphasis on sustainable development. Strategic positioning: Building a domestic base for high-end biological buffering agents The Huanggang New Factory project is committed to building a domestic high-end biological buffer production base, focusing on breakthroughs in key preparation technologies for a series of high-end biological buffers such as TRIS, HEPES, MOPS, etc. By constructing multiple intelligent production lines, the project will achieve a significant increase in the production of high-end biological buffering agents after it is put into operation, and it is expected that the annual output value will achieve a leapfrog growth. The construction of this base will effectively break the technological and market monopoly of foreign manufacturers in the field of high-end biological buffering agents. Anchor new goals and embark on a new journey The rapid progress of Huanggang's new factory construction is not only an important milestone in the development history of Xindesheng, but also a microcosm of China's intelligent manufacturing speed. From a blueprint to the initial scale of the factory building, from the groundbreaking ceremony to the topping out of the warehouse, the early completion of every milestone embodies the wisdom and sweat of all builders, demonstrating the corporate spirit of "pioneering innovation and pursuing excellence" of Xindesheng people. With the continuous advancement of the project, it will undoubtedly inject strong impetus into the future development of Xindesheng and contribute important strength to the high-quality development of the local economy.

For a long time, some studies have assumed that Bicine cannot interact with metal ions. However, an increasing number of facts indicate that Bicine can form stable binary or ternary complexes with metal ions, exhibiting certain chelating properties. The molecular structure of Bicine buffer is similar to that of glycine. This structure reduces the hydrolysis ability of the amino end, enhances overall stability, and also creates conditions for its binding with metal ions. Research has shown that Bicine can chelate with heavy metal ions such as copper (Cu), cadmium (Cd), and lead (Pb). However, this chelation effect is relatively weak and does not significantly interfere with the normal function of metal ions in most biochemical reactions, especially enzymatic reactions. In enzymatic reactions, metal ions often act as cofactors or coenzymes in the catalytic process, and their activity is highly influenced by various factors, with pH being one of the key factors. Extreme pH values can alter the binding state between metal ions and enzymes, thereby affecting catalytic activity. Bicine maintains pH stability in the solution while having weak chelation effect on metal ions, making it an ideal choice for enzymatic reactions that require the retention of metal ion activity. For example, in the serum guanine enzyme assay, the presence of Bicine can provide a stable pH environment for enzymatic reactions, and due to its weak binding properties with metal ions, it will not interfere with the role of metal ions in the reaction, ensuring the accuracy of the measurement results. From an application perspective, this characteristic of Bicine enables it to be widely used in many fields. In protein crystallization solution, Bicine can maintain the pH stability of the system without affecting the necessary interactions between proteins and metal ions due to excessive chelation of metal ions, which is beneficial for the growth and stability of protein crystals. Bicine acts as a buffer when extracting enzymes from various plant species, ensuring a suitable pH environment and maintaining metal ions in a free state that can participate in the composition of enzyme active centers or promote enzyme activity. In polymerase chain reaction (PCR), Bicine buffer system provides stable acid-base conditions for the reaction, while not hindering the reaction due to strong chelation of metal ions (such as magnesium ions, activators of Taq DNA polymerase in PCR reaction). Overall, the weak binding properties of the biological buffer Bicine with metal ions have more advantages than disadvantages in most biochemical and molecular biology applications. It provides a stable and suitable environment for numerous biochemical reactions that require the participation of metal ions, ensuring the authenticity and reliability of experimental results. With the continuous deepening of research and the expansion of application fields, fully understanding and reasonably utilizing the characteristics of Bicine and metal ions will inject new vitality into the development of related fields. The Bicine buffer produced by Hubei Xindesheng Material Technology Co., Ltd. has a low chloride ion content and all indicators meet relevant standards. In addition to Bicine buffer, Desheng actively researches and develops dozens of biological buffers such as TRIS and Hepes commonly used in the market. If you are interested, please click on the Desheng official website to learn more details! Overall, the weak binding properties of the biological buffer Bicine with metal ions have more advantages than disadvantages in most biochemical and molecular biology applications. It provides a stable and suitable environment for numerous biochemical reactions that require the participation of metal ions, ensuring the authenticity and reliability of experimental results. With the continuous deepening of research and the expansion of application fields, fully understanding and reasonably utilizing the characteristics of Bicine and metal ions will inject new vitality into the development of related fields. The Bicine buffer produced by Hubei Xindesheng Material Technology Co., Ltd. has a low chloride ion content and all indicators meet relevant standards. In addition to Bicine buffer, Desheng actively researches and develops dozens of biological buffers such as TRIS and Hepes commonly used in the market. If you are interested, please click on the Desheng official website to learn more details!  

Currently, China's primary healthcare system is undergoing unprecedented transformation and upgrading, leading to explosive growth in the field of in vitro diagnostics (IVD). This trend was confirmed at the recent "Million Talents Gathering in Southern Guangdong" medical and health job fair: 334 employers offered more than 6500 positions, with a particularly prominent demand for professionals in imaging, anesthesia, and other fields in county-level medical institutions. It is worth noting that the development of these departments is closely related to IVD technology, reflecting the strong demand for diagnostic testing in primary healthcare. The deep driving force behind the surge in demand The sustained release of policy dividends is the primary driving force. With the support of national policies, the hardware facilities of grassroots medical institutions have been comprehensively upgraded, and modern diagnostic and treatment equipment has been installed in township health centers and village clinics. Hardware improvements have created conditions for carrying out diversified testing projects, while the demand for accurate diagnosis among grassroots doctors has also skyrocketed. The rapid detection technology represented by POCT can precisely meet the dual pursuit of timeliness and accuracy at the grassroots level. The demographic changes bring new challenges. Global data show that the incidence rate of invasive fungal disease is increasing at an annual rate of 0.9%, and the elderly, especially those with chronic diseases, become vulnerable. The early identification of such diseases at the grassroots level highly relies on IVD technologies such as fungal culture and G-testing, which requires primary healthcare institutions to enhance their diagnostic capabilities. The deepening implementation of the hierarchical diagnosis and treatment system reshapes the pattern of medical treatment. With the promotion of the policy of first diagnosis at the grassroots level, grassroots medical institutions need to establish a more comprehensive diagnostic system. Fast and reliable IVD testing can not only help doctors timely assess the condition, but also provide scientific basis for patient referrals, effectively improving the efficiency of medical resource utilization. The deep driving force behind the surge in demand The sustained release of policy dividends is the primary driving force. With the support of national policies, the hardware facilities of grassroots medical institutions have been comprehensively upgraded, and modern diagnostic and treatment equipment has been installed in township health centers and village clinics. Hardware improvements have created conditions for carrying out diversified testing projects, while the demand for accurate diagnosis among grassroots doctors has also skyrocketed. The rapid detection technology represented by POCT can precisely meet the dual pursuit of timeliness and accuracy at the grassroots level. The demographic changes bring new challenges. Global data show that the incidence rate of invasive fungal disease is increasing at an annual rate of 0.9%, and the elderly, especially those with chronic diseases, become vulnerable. The early identification of such diseases at the grassroots level highly relies on IVD technologies such as fungal culture and G-testing, which requires primary healthcare institutions to enhance their diagnostic capabilities. The deepening implementation of the hierarchical diagnosis and treatment system reshapes the pattern of medical treatment. With the promotion of the policy of first diagnosis at the grassroots level, grassroots medical institutions need to establish a more comprehensive diagnostic system. Fast and reliable IVD testing can not only help doctors timely assess the condition, but also provide scientific basis for patient referrals, effectively improving the efficiency of medical resource utilization. Future Prospects Despite challenges such as talent shortage and quality control, the grassroots IVD market has enormous potential driven by policy support and technological progress. It is expected that in the coming years, with the integration and application of new technologies such as telemedicine and artificial intelligence, grassroots diagnostic services will develop towards a more intelligent and accurate direction, providing strong support for the construction of a healthy China. After years of development, Hubei Xindesheng Material Technology will uphold innovative concepts, continuously improve product quality and service levels, and contribute to the development of the IVD industry.  

Acridine esters, as an important class of chemiluminescent reagents, are widely used in fields such as immunoassay, nucleic acid detection, and biosensors due to their high sensitivity, rapid luminescence, and low background interference. However, its unique chemical properties require strict adherence to specific specifications during the dissolution process, otherwise it may lead to reagent deactivation or experimental failure. This article will start from the preservation characteristics of acridine esters and systematically analyze their scientific dissolution methods and operational points. Preservation characteristics of acridine ester: necessity of freeze-dried powder and low-temperature light avoidance Acridine esters are typically provided in the form of freeze-dried powder, which is designed to inhibit hydrolysis reactions and prolong reagent stability by removing moisture. The freeze-drying process can remove over 95% of the moisture, keeping the acridine ester molecules in an inactive state while avoiding aggregation or degradation caused by the presence of moisture. In addition, low temperature (usually -20 ℃ or lower) and light avoidance conditions are key to preserving acridine esters: low temperature can slow down molecular thermal motion and reduce hydrolysis rate; Avoiding light can prevent structural damage caused by photosensitive reactions. For example, acridine esters containing NHS (N-hydroxysuccinimide) groups are highly sensitive to both light and moisture, and exposure to room temperature or light for several hours can result in a loss of activity of over 50%. Selection of dissolution medium: necessity of non protonated solvents The dissolution of acridine esters requires avoidance of aqueous solutions, which is based on the unique chemical structure. The ester bonds and NHS groups in acridine ester molecules are highly susceptible to nucleophilic attack reactions with water molecules, leading to hydrolysis cleavage. Especially for acridine esters containing NHS groups, their hydrolysis half-life is only a few minutes to a few hours in water, but can be extended to several days or even weeks in non protonated solvents. Therefore, the following two types of solvents should be used to dissolve acridine esters: 1. Polar non proton solvents: such as dimethyl sulfoxide (DMSO) and N, N-dimethylformamide (DMF), which have no active hydrogen in their molecules and cannot provide protons to participate in hydrolysis reactions. At the same time, they can effectively dissolve the hydrophobic acridine ring structure of acridine esters. DMSO has become the most commonly used choice in laboratories due to its low toxicity, high boiling point (189 ℃), and good biocompatibility; DMF is suitable for the preparation of high concentration acridine esters due to its stronger solubility. 2. Mixed solvent system: For extremely insoluble acridine ester derivatives, a mixed solvent of DMSO and acetonitrile (ACN) or dimethylacetamide (DMA) can be used to promote dissolution by adjusting polarity. For example, mixing DMSO and ACN in a volume ratio of 7:3 can reduce the viscosity of the solution while maintaining its non protonated properties, making it easier for subsequent operations. Adaptation of application scenarios: from labeling reaction to luminescence detection The dissolved acridine ester solution can be directly used for chemiluminescence labeling of proteins, antibodies, or nucleic acids. For example, in immunoassays, acridine ester antibody conjugates can bind to antigens in aqueous buffer and subsequently trigger chemiluminescence reactions by adding hydrogen peroxide and sodium hydroxide. It is worth noting that the labeling reaction requires strict control of pH (usually 7.2-7.6) and ionic strength to avoid affecting the luminescence efficiency of acridine esters. Conclusion The dissolution of acridine ester is a key link connecting its stable storage and efficient application. By selecting non protonated solvents, standardizing operating procedures, and adapting to application scenarios, the chemiluminescence potential of acridine esters can be fully released, providing a highly sensitive and specific solution for biological detection. In the future, with the development of new anti hydrolysis acridine ester derivatives, their dissolution and application conditions are expected to be further optimized, promoting breakthroughs in chemiluminescence technology in more fields. As a manufacturer of luminescent reagents, Desheng is currently fully committed to supplying a range of high-quality acridine ester powders. These products are not only convenient to use, but also known for their luminescence sensitivity, ensuring that you can obtain accurate and reliable experimental results in a very short period of time. If you have purchasing needs or would like to learn more details, please feel free to click on our official website for consultation.  

Free Fatty Acids (FFA), as the core intermediate product of human energy metabolism, its abnormal level is closely related to diabetes, obesity, cardiovascular disease and metabolic syndrome. Accurately and rapidly measuring FFA concentration is not only a key indicator for clinical diagnosis, but also an important tool for drug development and nutritional assessment. Among numerous detection methods, the colorimetric substrate TOPS enzyme photometric method has gradually become the mainstream technology for FFA determination due to its high sensitivity, strong stability, and convenient operation. Technical principle: Perfect combination of enzymatic reaction and colorimetric reaction The core of TOPS enzymatic photometry is to link the enzymatic oxidation process of FFA with colorimetric reaction, and quantitatively analyze it through colorimetric method. Specifically, the detection process is divided into two steps: 1. Enzymatic oxidation stage: FFA in the sample is catalyzed by acetyl CoA synthase to bind with ATP and CoA to generate acetyl CoA, while releasing pyrophosphate; Subsequently, acetyl CoA oxidase further oxidizes acetyl CoA to produce hydrogen peroxide (H ₂ O ₂). 2. Color reaction stage: Under the catalysis of peroxidase (HRP), H ₂ O ₂ undergoes redox reaction with the color substrate TOPS and 4-aminoantipyrine (4-AAP) to generate stable red quinone imine compounds. The color depth of the compound is linearly related to the concentration of FFA, and the FFA content can be accurately calculated by measuring the absorbance with a spectrophotometer (usually at a wavelength of 550-570nm). Technical advantages: sensitive, stable, easy to operate Compared with traditional methods, TOPS enzyme photometry exhibits significant advantages: 1. High sensitivity: TOPS has a much higher molar absorptivity than traditional color reagents (such as copper reagents), and can detect FFA as low as μ mol/L. It is particularly suitable for precise analysis of low concentration samples such as serum. 2. Strong stability: TOPS has stable chemical properties and is not easily affected by temperature, pH fluctuations, or sample matrix interference, ensuring the repeatability and reliability of detection results. In addition, the kit format (dual reagent system) further simplifies the operation process and reduces human errors. 3. Convenient operation: No complex preprocessing steps (such as organic extraction or derivatization) are required. The sample can be directly mixed and incubated with the reagent, and the detection can be completed in 10-15 minutes, greatly reducing the detection time. 4. High cost-effectiveness: Compared with gas chromatography or liquid chromatography, TOPS enzyme photometry does not require expensive instruments and equipment, has low reagent consumption, and is suitable for large-scale promotion in clinical laboratories. Clinical Application: From Disease Diagnosis to Health Management The application scenarios of TOPS enzyme photometry are extensive, covering multiple fields such as clinical diagnosis, metabolic research, and drug development 1. Cardiovascular disease risk assessment: The increase of FFA level is an independent risk factor for atherosclerosis and myocardial ischemia. Regular detection can assist in early diagnosis and prognosis monitoring. 2. Metabolic disease management: diabetes patients are often accompanied by FFA metabolic disorder. Dynamic monitoring of FFA concentration can optimize insulin treatment programs and control blood sugar fluctuations. Conclusion: The TOPS enzyme photometry method, based on innovative scientific principles and practical technical implementation, provides an efficient and accurate solution for FFA detection. From clinical diagnosis to health management, from basic research to drug development, this technology is continuously driving the progress of metabolic medicine and safeguarding human health. The TOPS and other the new Trinder's reagents produced by Hubei Xindesheng Material Technology Co., Ltd. have high purity, good water solubility, stable production process, and small inter batch differences. All indicators meet relevant standards. If you have any purchasing needs in the near future, please feel free to contact me or click on the official website for more details!  

In biological and biochemical experiments, biological buffering agents serve as the core reagent for maintaining solution pH stability, and their sterilization treatment directly affects the accuracy and reliability of experimental results. From cell culture to protein purification, from nucleic acid research to drug development, the sterilization decision of buffer solution needs to be comprehensively judged based on experimental type, reagent characteristics, and operating standards. The necessity of sterilization: the type of experiment determines the risk level 1. Rigid requirements for high-sensitivity experiments In experiments such as cell culture, gene editing, or single-molecule testing that require high levels of sterility, microbial contamination may lead to cell death, abnormal gene expression, or signal interference. For example, although MOPS buffer is commonly used for RNA electrophoresis, its pH stability is easily affected by microbial metabolites. If the experiment involves rare samples or long-term cultivation, high-pressure sterilization can effectively eliminate potential sources of contamination. Similarly, after high-pressure sterilization, the pH stability of BES buffer solution is significantly improved, making it suitable for cell experiments sensitive to ion strength. 2. Stability guarantee for long-term storage Unterilized buffer solutions may breed microorganisms during storage, leading to pH drift or precipitation. For example, phosphate containing buffer solutions are prone to form insoluble complexes with calcium and magnesium ions at high temperatures, and sterilization treatment can delay this process. For Tris HCl buffer solutions that require long-term storage, high-temperature and high-pressure sterilization can ensure their chemical stability and avoid degradation of active ingredients caused by microbial growth. 3. Avoid external protein interference The proteins contained in microorganisms themselves may interfere with experimental results through cross reactions. In Western Blot experiments, if the buffer is not sterilized, bacterial proteins may undergo non-specific binding with the target protein, leading to false positive signals. Sterilization treatment can completely remove such interference sources, especially suitable for low abundance protein detection or high specificity antibody incubation scenarios. Practical considerations for sterilization: balancing cost and benefit 1. Experimental cost and operational complexity High pressure sterilization requires specialized equipment and takes a long time, while membrane filtration, although fast, requires regular replacement of the membrane. For large-scale experiments, membrane filtration may increase the cost of consumables; For small-scale or high-value experiments, the benefits of sterile assurance far exceed the costs. For example, in the production of gene therapy vectors, the use of sterilization buffer can avoid batch contamination risks and ensure product safety. 2. Supplementary role of operating standards Even if the buffer has been sterilized, sterile operating procedures must still be followed throughout the experiment. For example, using sterile pipette tips during the transfer process and operating in ultra clean workbenches can further reduce the risk of contamination. In addition, experimenters wearing lab coats, gloves, and goggles can avoid secondary contamination of the buffer solution by human microorganisms. Conclusion: Scientific decision-making and precise sterilization The sterilization treatment of biological buffering agents is not a one size fits all approach, but requires comprehensive decision-making based on the type of experiment, reagent characteristics, and operating conditions. Sterilization is a necessary means to ensure the reliability of results for high-sensitivity experiments, long-term storage, or scenarios involving external protein interference; For routine experiments or thermosensitive buffering agents, membrane filtration or strict aseptic operation can replace high-pressure sterilization. By scientifically selecting sterilization methods and standardizing operating procedures, the performance of biological buffering agents can be maximized, providing solid support for biological research. Hubei Xindesheng Material Technology Co., Ltd. is a manufacturer of diagnostic reagent raw materials, which can provide various biological buffering agents, including Tris, Tris HCl, Bis Tris, Bicine, TAPS and other reagents. If you need to purchase, please feel free to contact us at any time!  

On the morning of September 3, 2025, Hubei Xindesheng Material Technology Co., Ltd. organized all employees to watch the live broadcast of the grand September 3 military parade, witnessing the magnificent strength of the country and feeling the spirit of the times together. The activity aims to deepen patriotic education, enhance team cohesion, and inspire employees' sense of national pride and professional mission.   At 9 o'clock in the morning, the company conference room was filled with a solemn and enthusiastic atmosphere. All employees hold bright red flags issued by the company and watch the parade on the big screen with focused expressions. When the imposing troops marched with resolute steps, when advanced weapons and equipment appeared one by one, and when the fighter jets soaring in the blue sky traced magnificent paths, sincere admiration and warm applause erupted from the scene time and time again. The employees waved the red flag in their hands, sincerely feeling proud and honored for the increasingly strong comprehensive national strength and glorious achievements in national defense construction of the motherland.   Chairman Wang Zhongxi passionately elaborated on the profound enlightenment brought by watching the military parade in his speech. He first mentioned emotionally that the resolute figure of all the soldiers who stood still for hours in the scorching heat is a reflection of the precious craftsmanship spirit of this era. Behind this perseverance is the noble choice of integrating personal values into the destiny of the country. The people of Xindesheng should also cherish their country and closely link their personal growth with the development of enterprises and the country. Mr. Wang also pointed out that the grand military parade demonstrated the prosperity and stability of the country, which is the solid foundation for enterprises to operate with peace of mind and continue to develop. New Desheng must integrate its own development into the overall development of the country and repay the opportunities given by the times with practical actions.   Chairman Wang Zhongxi called on all employees of Xindesheng to transform the surging enthusiasm and deep patriotism inspired by watching the military parade into practical actions that are down-to-earth. He demands that everyone deeply study and understand the spirit of the military parade, which embodies perseverance, loyalty, collaboration, and responsibility, and inject it into every research and development project, every production process, and every market service. With a more vigorous fighting spirit and an attitude of striving for excellence, we are committed to innovation and breakthroughs in the field of IVD. With outstanding work performance and continuously rising corporate achievements, we contribute our wisdom and strength to the prosperity of the local economy and the technological progress of the industry. We turn this ambitious mission of serving the country into a sincere and moving gift to our great motherland.   Hubei Xindesheng is currently in a new stage of rapid development. The construction of a new production base in Huanggang has entered an accelerated stage, aiming to create a production base for high-end biological buffering agents in China, in order to break through import dependence and achieve domestic substitution of key raw materials. We firmly believe that Hubei Xindesheng will transform the pride and sense of mission inspired by the military parade into an inexhaustible driving force for promoting the independent and controllable development of the IVD industry chain with high quality. With higher quality innovative products and outstanding industry contributions, we will serve our ancestors  

In recent years, the in vitro diagnostic (IVD) industry in China has encountered unprecedented development opportunities driven by multiple factors such as the continuous growth of global medical demand, technological progress, and population aging. By 2025, the size of China's IVD market is expected to exceed 135 billion yuan, becoming an indispensable and important component of the modern healthcare system. Industry status: Steady growth, diverse highlights in segmented fields The IVD industry in China has shown a steady growth trend in recent years. According to market research institutions, the size of China's IVD market has reached 125 billion yuan in 2024, a year-on-year increase of 8.5%. It is expected that by the end of 2025, the market size will exceed 135 billion yuan, with a growth rate maintained at around 8%. In the segmented field, immunodiagnosis still holds the largest market share, thanks to the continuous development of technologies such as chemiluminescence and electrochemiluminescence. Its applications in infectious diseases, tumor markers, thyroid function and other detection projects are becoming increasingly widespread. Molecular diagnostics, as the fastest growing sub field, has an average annual growth rate of about 20%. The continuous innovation of technologies such as PCR, NGS, dPCR, etc. has promoted their widespread application in infectious disease diagnosis, tumor gene testing, and other fields. The POCT market, with its fast and convenient characteristics, has a strong demand in primary healthcare, emergency treatment and other scenarios, and is expected to maintain a high growth rate in the coming years. Technological innovation: the core driving force for industry development Technological innovation is a key factor driving the development of the IVD industry. In the field of immunodiagnosis, the integration of chemiluminescence technology with microfluidics and AI has become an important trend, greatly improving the sensitivity and automation level of detection. In the field of molecular diagnostics, the continuous optimization and upgrading of PCR technology, especially the application of digital PCR (dPCR), has further improved the accuracy of detection, providing strong support for tumor liquid biopsy, rare disease gene detection, and so on. POCT technology is developing towards miniaturization, intelligence, and multi index joint inspection. By combining IoT and AI technology, it achieves real-time transmission and intelligent analysis of detection data, meeting the needs of primary healthcare, home self inspection, and other scenarios. Competitive landscape: Market reshuffle under diversified situation The competitive landscape of China's IVD industry is showing a diversified trend, with foreign-funded enterprises and local enterprises competing on the same stage. Foreign funded enterprises dominate the high-end market with advanced technology and brand advantages, but with the continuous breakthroughs in local enterprise technology and the improvement of product quality, their market share is gradually being squeezed. Local enterprises, relying on cost advantages, in-depth understanding of the local market, and policy support, occupy a large share in the mid to low end market and continuously penetrate into the high-end market. The implementation of centralized procurement policy and the promotion of medical insurance payment reform have accelerated market reshuffle, putting greater pressure on small and medium-sized enterprises to survive, and further concentrating market share towards top enterprises. Future trend: Emerging markets and international development become new growth points Looking ahead, the IVD industry in China will continue to maintain a stable growth trend. With the continuous growth of medical demand, the promotion of technological innovation, and the optimization of policy environment, the industry scale will continue to expand. Emerging markets such as pet medical testing and consumer health testing will become new growth points, bringing new development opportunities to the IVD industry. As an upstream IVD reagent raw material manufacturer, Hubei Xindesheng Company can supply a variety of raw materials suitable for molecular diagnostics and other fields, including biological buffering agents, luminescent reagents, enzyme reaction substrates, etc. Hubei Xindesheng Material Technology Co., Ltd. has always maintained the research and development production philosophy of "exploring and innovating, pursuing excellence" in the face of current challenges and opportunities. At the same time, it actively expands overseas markets, participates in international competition, and enhances its international market share.    

In biochemical experiments, CAPS buffer, as an important alkaline buffer, is widely used in Western blotting, enzyme catalyzed reactions, and HPLC separation due to its stable pKa value (about 10.4), good water solubility (up to 11.07 mg/mL at 25 ℃), and low cell membrane permeability. However, experimenters often find that the solubility of CAPS significantly decreases at low temperatures (such as 4 ℃ or -20 ℃), leading to difficulties in buffer preparation or uneven concentration, which in turn affects the reliability of experimental results. This article will analyze this phenomenon from three levels: molecular mechanisms, environmental factors, and experimental operations, and propose targeted optimization solutions. Molecular mechanism of low temperature solubility decrease The dissolution process of CAPS is essentially the process of its molecules forming a hydration layer with water molecules through hydrogen bonding. At room temperature, the sulfonic acid group (- SO3H) and amino group (- NH -) in CAPS molecules combine with water molecules through polar interactions to form stable solute solvent complexes. However, as the temperature decreases, the thermal motion of water molecules weakens, and the hydrogen bonding network tends to become rigid, resulting in a decrease in the binding energy between CAPS molecules and water molecules. Experimental data shows that the solubility of CAPS decreases by about 30% at 4 ℃ compared to 25 ℃, which is closely related to changes in the dynamic properties of water molecules. In addition, the crystallization behavior of CAPS undergoes significant changes at low temperatures. At room temperature, CAPS molecules are dispersed in a disordered state in solution; When the temperature drops below the critical point, molecules form an ordered lattice structure through hydrophobic interactions and π - π stacking. This phase transition process will further reduce the solubility of CAPS and even lead to the precipitation of undissolved solid particles. For example, when preparing CAPS buffer solution, if the solvent is not sufficiently preheated, white flocculent precipitates can often be observed, which is a direct manifestation of low-temperature induced crystallization. The synergistic effect of environmental factors on solubility In solvent ion strength experiments, deionized water is often used to prepare CAPS buffer solution. However, if there are residual metal ions (such as Ca ² ⁺, Mg ² ⁺) in the water, they will form complexes with the sulfonic acid groups in CAPS molecules, reducing their effective solubility. At low temperatures, the hydration of ions is enhanced, the stability of the complex is improved, and further exacerbating the decrease in solubility. For example, in a solution containing 0.1 mM Ca ² ⁺, the solubility of CAPS at 4 ℃ is reduced by 15% compared to pure water system. The solubility of CAPS is closely related to its dissociation state due to pH fluctuations. When the pH is below pKa (10.4), CAPS molecules exist in protonated form (- SO3H) with high solubility; When the pH approaches or exceeds pKa, the solubility of the deprotonated form (- SO ∝⁻) significantly decreases. Under low temperature conditions, the pH value of the buffer solution may drift due to the dissolution of CO ₂ or hydrolysis of impurities, indirectly affecting the dissolution behavior of CAPS. By understanding the molecular mechanism and environmental factors that contribute to the decrease in low-temperature solubility of CAPS, researchers can optimize the preparation process and storage conditions in a targeted manner to ensure the stability of the buffer performance. In the future, with the development of new zwitterionic buffering agents, the limitations of CAPS in low-temperature experiments are expected to be fundamentally resolved. As a professional biological buffering agent manufacturer, Desheng is committed to providing high-quality CAPS buffering agents. We not only have professional personnel to supervise and control the process from raw material use to factory preparation, but also continuously optimize testing methods to meet the diverse needs of our customers. There is stock available for sale in the warehouse at a cheap price. If you have any relevant intentions, please feel free to click on the website to inquire about details and purchase at any time!

In cell culture and biological experiments, HEPES buffer and phenol red indicator are often used simultaneously. The former is used to maintain pH stability in the culture medium, while the latter is used to visually display pH changes. However, the interaction between the two in terms of chemical properties may lead to color abnormalities, affecting the accuracy of experimental results. This article will analyze the causes of this phenomenon and provide simplified optimization solutions. Chemical basis of color interference Phenol red is a pH sensitive dye that changes color with acidity or alkalinity: it appears yellow in acidic environments (pH8.2). This characteristic makes it an ideal tool for monitoring the pH of cell culture media. The core function of HEPES as a buffer is to stabilize pH by releasing or absorbing hydrogen ions. The problem is that the sulfonic acid groups in HEPES molecules have a similar chemical structure to phenol red, and when the concentration of HEPES is high, they will interact with phenol red to change its molecular structure. This change causes the color of phenol red to become lighter or shift at a specific pH, for example, it may appear orange red instead of standard red at pH 7.4. The intuitive manifestation of interference phenomenon In conventional cell culture, if high concentrations of HEPES (such as over 20mmol/L) and phenol red are used simultaneously, the color of the culture medium may be lighter or yellowish than expected. For example, the pH 7.4 culture medium, which should have displayed red, may have turned pale orange due to HEPES interference, leading researchers to misjudge the pH value. In fluorescence microscopy observation, this interference is more pronounced. Phenol red emits fluorescence at specific wavelengths, while HEPES may absorb some of the fluorescence signal, resulting in decreased image brightness or increased background noise. This effect is particularly prominent in long-term observation or live cell imaging experiments, which may mask the true state of cells. Simplified optimization plan Adjust HEPES concentration 1. Conventional cultivation: Control the concentration of HEPES within 10-15 mmol/L, which has minimal interference with the color development of phenol red and can effectively maintain pH stability. 2. Short term experiment: If the experimental time is short (such as

In the use of the new Trinder's reagent TODB, high background is a common problem that affects the detection results, while insufficient sealing and incomplete plate washing are key factors that are easily overlooked during operation. These two issues can cause an abnormal increase in the color background due to non-specific binding and interference from residual substances. Below is a detailed analysis and solution. Inadequate closure: the 'behind the scenes' driving force behind non-specific combinations The function of the blocking step is to block the non binding sites on the surface of the reaction carrier (such as an enzyme-linked immunosorbent assay plate). If the sealing is not sufficient, the substrates, enzyme conjugates, and other components in the TODB reagent will randomly adsorb onto the surface of the carrier, forming a color signal without the participation of the target substance, directly increasing the background value. specific reason 1. Insufficient sealing time: Generally speaking, sealing requires placing at 37 ℃ for 60 minutes or at room temperature for 120 minutes. If the time is shortened to less than 30 minutes, the active sites on the surface of the carrier cannot be completely covered by the blocking solution (such as BSA, skim milk powder), and those hydrophobic areas that are not blocked will actively adsorb protein components in the TODB reaction system, resulting in background coloration. 2. Too low concentration of blocking solution: When the effective ingredients in the blocking solution are insufficient, such as when the original 5% BSA drops to 1%, a tight protective film cannot be formed between molecules. Components such as horseradish peroxidase in TODB reagent will stick to the inner wall of the plate pore through hydrophobic interactions, and non-specific reactions will occur with the substrate during the reaction. 3. Failure of sealing solution: If the sealing solution is repeatedly frozen and melted, or stored for more than its expiration date, the protein components inside will deteriorate and lose their sealing function, resulting in many "exposed" sites on the surface of the carrier, which become the source of background signals. Incomplete board washing: the "stacking effect" of residual substances The main function of plate washing is to remove unbound free reagents (such as unbound antibodies, TODB precursor substances). If the plate washing is not thorough, residual substances will continue to participate in color development in subsequent reactions, allowing background interference to accumulate. Specific Reason 1. Too few plate washes: Conventional testing requires washing the plate 3-5 times. If it is reduced to less than 2 times, the residual free enzyme complexes in the well cannot be completely removed, and the reaction will react with the TODB substrate, resulting in additional color development. 2. Too much detergent residue: After washing the board, if the holes are not inverted on the absorbent paper and patted dry, there will be more detergent residue in each hole, and some components inside will disrupt the balance of the TODB reaction system. At the same time, residual enzyme conjugates will diffuse into the adjacent holes with the liquid, causing cross contamination and raising the background. 3. There is a problem with the washing machine: When the needle of the washing machine is blocked or the pressure is insufficient, the corners of the plate holes will become areas that cannot be washed. The residual TODB reagent will crystallize after drying and participate in the reaction when dissolved again, causing the local background color to darken. Summary: Operational details determine the effectiveness of background control Although sealing and washing plates are routine steps, their quality directly affects the background level of TODB reagents. In practical use, it is recommended to adopt the method of "extending the sealing time+increasing the number of plate washes", combined with measures such as checking the concentration of the sealing solution and regularly calibrating the washing machine, which can significantly reduce the background value and provide assurance for the accuracy of the detection results. Desheng specializes in producing more than ten new Trinder's reagents, including TODB. After more than ten years of research and development, it can ensure that TODB appears as a powder with a purity of up to 99.5%, strong water solubility, and stable performance to ensure the accuracy of experimental results. Desheng has a place in the market for in vitro diagnostic kit raw materials with high-quality products, and is deeply trusted and supported by customers at home and abroad. If you have any relevant intentions, please click on the official website for consultation!  

In the field of biochemical detection, the selection of colorants plays a crucial role in the sensitivity, accuracy, and stability of detection methods. At present, there are various types of colorants in the market, such as TMB widely used in enzyme-linked immunosorbent assay (ELISA), which has become a common choice for many detection scenarios due to its wide applicability. However, in the detection of specific indicators, the new Trinder's reagent has demonstrated unique and excellent performance, with TOPS reagent showing significant advantages in the determination of fatty acids. Outstanding advantages in sensitivity and accuracy In biological detection, sensitivity and accuracy are the core indicators for measuring the quality of detection methods. When using TOPS reagent to determine serum free fatty acids (FFA), its sensitivity is extremely excellent. The content of serum free fatty acids in the body is relatively low, and even small changes in their concentration may be closely related to various physiological and pathological states. TOPS reagents can accurately capture these subtle concentration changes, and even low concentrations of serum free fatty acids can be effectively detected, providing more accurate data support for clinical diagnosis and research. Meanwhile, TOPS reagents are equally accurate. During the detection process, it can minimize the influence of interfering factors and ensure that the detection results truly and reliably reflect the content of free fatty acids in the serum. Compared with some traditional colorants, the detection results of TOPS reagent have higher repeatability and consistency, effectively avoiding errors caused by unstable properties of colorants, and providing a solid basis for scientific research and clinical decision-making. Easy and convenient operation In addition to high sensitivity and accuracy, the ease of operation is also a major highlight of TOPS reagents. In practical testing, the method of using TOPS as a chromogenic substrate to detect serum or plasma FFA is relatively simple in steps, without the need for complex instrument equipment and cumbersome operating procedures. Researchers or clinical laboratory personnel only need to follow the standard experimental operation guidelines, mix the sample with TOPS reagents and related reagents, and after an appropriate reaction time, detect it using conventional instruments such as spectrophotometers. This simple and convenient operation method not only improves detection efficiency and reduces experimental costs, but also reduces errors caused by operational errors, making the detection results more reliable. Excellent performance in multiple standards Among numerous Trinder's colorants, TOPS reagent stands out on multiple key performance indicators. Secondly, TOPS reagents have high stability. During storage and use, it is not easily decomposed or deteriorated, and can maintain its chemical stability for a long time, providing reliable assurance for detection. In addition, the molar absorptivity of TOPS reagent is high, which means that it has strong light absorption ability and can produce significant color changes at lower concentrations, thereby improving the sensitivity and detection limit of the detection. In summary, the new Trinder's reagent TOPS has significant advantages in determining fatty acids, such as high sensitivity, good accuracy, simple and convenient operation, and excellent performance indicators. With the continuous development of biological detection technology, TOPS reagents are expected to play a more important role in the field of fatty acid detection, providing stronger support for life science research and clinical diagnosis. Desheng specializes in producing more than the new Trinder's reagents, including TOPS. After more than ten years of research and development, it can ensure that TOPS appears as a powder with a purity of up to 99.5%, strong water solubility, and stable performance to ensure the accuracy of experimental results. Desheng has a place in the market for in vitro diagnostic kit raw materials with high-quality products, and is deeply trusted and supported by customers at home and abroad. If you have any relevant intentions, please click on the official website for consultation!