Glycerol, also known as glycerol, is colorless, sweet, clear, viscous liquid, odorless, warm and sweet. It can absorb moisture from the air, as well as hydrogen sulfide, hydrogen cyanide and sulfur dioxide. It is insoluble in benzene, chloroform, carbon disulfide, petroleum ether and oil. Glycerol is the backbone of triglycerides.
When the human body ingests edible fat, triglycerides are metabolized in the body to form glycerol and stored in fat cells. Therefore, the end products of triglyceride metabolism are glycerol and fatty acids.
At present, the main components of Virus Transport Media are Hank's solution, gentamicin, fungal antibiotics, BSA (the fifth component of bovine serum albumin), biological buffer Tris, amino acids, cryoprotectant, glycerol and other components. Among them, glycerol has the function of nutrition and desiccant. Virus resistance to the outside world is not strong, sensitive to temperature. Glycerol of 50% concentration makes the preservation in a relatively static state.
Desheng has developed two kinds of preservative solutions according to market demand: inactivated and non inactivated, which can be used for the collection, preservation and transportation of specimens of nasopharyngeal pathogens such as new coronavirus, influenza, avian influenza, hand foot mouth disease, measles, etc. The inactivated type contains lysate, which can instantly cleave pathogens and release nucleic acid, and the protective agent can prevent nucleic acid from being degraded. At present, the inactivated type is commonly used in the detection of NCNA, which greatly reduces the risk of infection. The non inactivated type does not contain lysate, can maintain the activity and integrity of pathogens, and can be used for virus culture and isolation.
Inactivated Virus Transport Media can fully mix the collected samples with virus lysate and virus nucleic acid preservation solution to inactivate the virus in the samples, effectively ensure the integrity of virus nucleic acid in the samples, and the collected samples can be transported under normal temperature and stored for a long time. The preserved virus RNA samples can be widely used in gene detection, enzyme-linked immunosorbent assay (ELISA), PCR detection and so on.
On the basis of Hank's, the non inactivated Virus Transport Media is added with BSA (bovine serum albumin) amino acids, vitamins and other nutrients needed by the virus, which can maintain the activity of the virus in a wide temperature range and maintain the original sample to the greatest extent. It can be used for virus nuclear acid extraction detection, virus culture and isolation.
Desheng began to develop Virus Transport Media at the early stage of the epidemic. After the overtime work of the company's R & D personnel and many experiments, the product was finally developed successfully, and the product was highly praised by customers after use. Now we have reached a stable customer base of dozens. The temperature is gradually decreasing, and winter is coming. Experts predict that the new coronavirus may attack again. In order to strictly prevent the new coronavirus, the Virus Transport Media for nucleic acid detection is essential.
