China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Desheng Technology is a new technology enterprise specializing in the research and development, production and sales of blood collection tube additives. It has 15 years of experience in the production of blood collection tube additives. The main components of Desheng's blood coagulant are composed of thrombin, alcohol, silica powder, emulsifier, surfactant, etc., which are suspended liquids.     Serum is one of the main specimens tested in clinical biochemical and immunological tests. At present, the methods of obtaining serum samples in medical institutions are mainly obtained by centrifugation after collecting venous blood after the blood is completely coagulated. Under normal circumstances, it takes more than 60 minutes for an isolated blood sample to completely coagulate, or even not coagulate, which is difficult to meet the needs of rapid laboratory testing.   Under normal circumstances, it takes a long time for the collected blood sample to coagulate naturally at room temperature (2-35℃). Generally, it takes more than 60 minutes for glass tubes and more than 90 minutes for plastic tubes. Since the clinical needs laboratory to provide rapid and accurate laboratory biochemical, immunological and other testing indicators, if the collected blood samples are not processed, it is difficult to meet the clinical needs in time, especially for emergency patients, it is necessary to obtain fast and accurate test results. . The traditional method of promoting blood coagulation is mainly to add kaolin and cephalin to blood samples after collection that can promote blood coagulation. However, with the advancement of clinical laboratory testing methods, some automated and intelligent biochemical and immunological Testing instruments are constantly updated and used for clinical testing and analysis. While the sensitivity and accuracy of these automatic analysis instruments continue to improve, the requirements for specimens are also increasing.   Procoagulant principle of blood coagulant: Activate part of the coagulation factors in plasma and platelets, promote the formation of thromboplastin and fibrin clots, accelerate the contraction of blood clots, and achieve the purpose of rapid serum separation.   Usage and dosage of coagulant: The accelerator can be sprayed by machine or added manually. In the process of adding, it must be stirred continuously to keep the suspension uniform and fully ensure the consistent addition of various active ingredients.   2. Mechanical stirring, electromagnetic stirring, pneumatic stirring, etc. can be used to keep the suspension uniform, and the stirring speed can be adjusted according to actual conditions. 3. After the addition is complete, the coagulant needs to be dried (not needed for non-organic solvent coagulant). The hot air drying temperature can be 40-80℃, and the time is 7-120S; the drying temperature in the drying oven or drying room is about 40C, and the time is subject to thorough drying.   4. Add 20-30ul blood coagulant, it can quickly coagulate 2-5ml blood at 20-25℃. When the ambient temperature is lower than 20°C, or the blood volume is more than 5ml, the amount of coagulant added should be appropriately increased. If irradiation sterilization is required, it is recommended to increase the additive dosage of coagulant by about 20%.   Blood coagulant accelerates the speed of blood coagulation and provides convenient conditions for testing. Desheng is one of the few manufacturers that produces a full set of additives for blood collection tubes. The additives produced include heparin sodium/lithium, EDTA dipotassium/tripotassium, coagulant and sodium citrate, potassium oxalate, separation gel, etc., which are purchased for customers Provided convenience.

In chemiluminescence immunoassay experiments, there are two types: plate and tube. Among them, tube chemiluminescence will use magnetic beads as a carrier for adsorbing acridinium ester-labeled antibodies or proteins to facilitate the washing and separation of labeled antibodies. The magnetic beads here are a kind of magnetic biological microspheres, which are nanomaterials. In fact, there are many types of nanomaterials in the in vitro diagnostic reagent industry. The magnetic beads newly developed by the company are different from the original serum separation gel and high molecular polymer materials such as carbomer. Magnetic beads are a kind of more advanced nano materials. Because nanomaterials have unique optical, magnetic, electrical and thermal properties, they can be used to generate different types of detection signals, amplify the intensity of detection signals, and simplify the detection process. Therefore, in vitro diagnostic technology based on nanomaterials has broad application prospects. Nanomaterials can be applied to the detection of nucleic acids, proteins, small molecules, bacteria and viruses. In Vitro Diagnostic Reagents and Magnetic Bead Materials With the rapid development of clinical medicine diagnosis and treatment technology, many traditional and conventional in vitro diagnostic technologies can no longer meet the needs of clinical medicine development. People have higher and higher requirements for the sensitivity, accuracy and specificity of clinical diagnostic analysis. Therefore, nanomaterials Also came into being. In particular, because nanomaterials have a much higher specific surface area than macroscopic materials, they can provide a lot of space to modify different molecules on their surfaces, making them play an important role in applications such as bioanalysis and biosensors. Using these surface-modified nanomaterials with different molecules can selectively detect small molecules, nucleic acids, proteins and microorganisms. There are many different types of nanomaterials, such as semiconductor quantum dots, gold nanoparticles, and magnetic nanoparticles. Among them, magnetic beads are a kind of magnetic nanoparticles, which are widely used in the field of in vitro diagnostic reagents. Nanomaterials can be used for the detection of nucleic acids and antibody proteins in chemiluminescence immunity according to different types; some can be used for enzyme-linked immunosorbent assay (ELISA) immunoassays and DNA microarray detection, etc.; others are used for tumor cell detection and bacteria Detection, virus detection, and tracing of small molecule drugs in living cells, etc., have a very broad application prospects. In the past, acridine esters chemiluminescence reagents mainly relied on imports. Now, due to the good market feedback of the acridine esters developed by Desheng, the magnetic bead materials developed are mainly used for chemiluminescence experiments. Other in vitro diagnostics will be considered later. Application in experiment.

Carbomer is a white, loose powder with strong hygroscopicity and acidity. It is soluble in water, glycerin, and ethanol. It has the characteristics of a colloidal solution. The usual concentration is 0.1% to 3%. Due to the low viscosity, when the alkali is neutralized, the viscosity gradually rises as the macromolecules gradually dissolve, forming a clear solution when the mass fraction is low, and a translucent gel when the mass fraction is large. Carbomer not only It is widely used in daily chemicals. It can also be used as a promising new pharmaceutical excipient. The following introduces some problems that should be paid attention to when using carbomer to make gels.   Carbomer model selection   According to the different uses of the prepared gel, choose different types of CP. CP934 has the strongest adhesion and is more suitable for cavity administration, such as commonly used nasal bioadhesives, vaginal bioadhesives, rectal bioadhesives, oral Containing or oral bioadhesives, etc. CP940 has strong adhesion to the skin surface and good spreadability, which can prolong the residence time of the drug on the skin surface and increase the effect of the drug. It is widely used in skin diseases and can absorb tissue exudate, which is beneficial to the exclusion of secretions. Suitable for treatment of seborrheic skin diseases. CP1342 has strong ion resistance, which is more suitable in the presence of electrolyte.   Carbomer quality score selection   According to the different viscosity of the required gel, generally choose the mass fraction of CP from 0.5% to 3%. When formulating skin care products, the final mass fraction of CP should be controlled to be less than or equal to 0.8%. When it is greater than 0.8%, it is easy to form a film and the skin feels bad. In order to achieve the desired consistency, CP can also be used in combination with carmellose, sodium alginate, polyethylene glycol, polyvinylpyrrolidone, etc. to achieve a synergistic thickening effect.   Choice of pH regulator   In order to make CP form a more stable gel, its pH needs to be adjusted from 5 to 11. The CP neutralizer can be sodium hydroxide, potassium hydroxide, potassium bicarbonate, borax and triethanolamine in polar systems, and laurylamine and amine stearate can be used in non-polar systems.   Choice of moisturizer   Since the gel will lose water during storage, it will dry and harden, forming a dry micelle which is not easy to spread. It is necessary to add a moisturizer to maintain the stability of the dosage form and prevent the skin from drying out. Commonly used humectants include polyols such as glycerin and propylene glycol.   Selection of preservatives   Carbomer itself has no nutrition and does not support the growth of bacteria and molds, but it cannot prevent the growth of bacteria and molds using the nutrients present in the gel system, so different preservatives should be selected according to the situation, such as sodium benzoate and p-hydroxybenzene Formate (parabens) and ethanol, etc. Preservatives can also be combined to reduce dosage and achieve synergistic antibacterial effects.   As a professional carbomer manufacturer, Desheng can provide two models of carbomer 940, carbomer 980, high-quality products and services, and higher cost performance than other manufacturers. Desheng has always been favored by customers. This is definitely the driving force for Desheng to move forward. In order to thank new and old customers, we are now launching = a year-end promotional event, the price is as low as 100 yuan/kg, and the opportunity is not to be missed. Please come to consult and order!

  The full chemical name of Hepes free acid (CAS number: 7365-45-9) is N-(2-hydroxyethyl)-piperazine ethane sulfonic acid (hereinafter referred to as "HEPES") is a zwitterionic biological buffer. It has physical properties including a white powdery appearance at room temperature and its excellent water solubility. 40 grams of HEPES can be completely dissolved in 100 ml of pure water at 20°C. Therefore, it is very easy to use and only needs to be dissolved in water. The scope of application is relatively wide, usually using its pH value for adjustment, cosmetics, cell culture, electrophoresis buffer, etc. HEPES handling and storage recommendations   HEPES powder is resistant to high temperatures and has a melting point of up to 200°C, so it will not be degraded by autoclaving. According to relevant literature, if the HEPES water solution is exposed to ambient light for three hours, cytotoxic hydrogen peroxide (H 2 O 2) will be produced. Therefore, it should be noted that the HEPES water solution must be kept away from light to avoid affecting the experimental results.   Why choose HEPES   As a professional manufacturer of biological buffers, Desheng provides high-quality HEPES according to your application. We also provide a variety of packaging options, and some indicators can be customized according to your needs. Desheng has a professional R&D team, which is very different from a company that only sells or produces. Desheng is based on research and development, and it is our requirement to produce high-quality products.   Desheng HEPES packaging and storage   The conventional HEPES package of Desheng is a cardboard drum of 25kg, lined with a plastic bag, the white powder is packed in the belt, and the tie is tight. When placed, it is generally placed in a dry room and cannot be exposed to direct sunlight for a long time. Standing inventory, the supply is relatively stable. It is best to store at room temperature 2-8℃.   In the fierce market competition, Hubei Xindesheng always adheres to the concept of "quality first, technology leading", strictly controls every batch of goods sold, from the purchase of raw materials to the control of production processes, to product packaging, logistics transportation, and after-sales service, all supervised by professional personnel. So far, in addition to exporting Hepes buffer, Desheng also has dozens of biological buffers for sale. If you are interested, please click on the official website to learn more details!  

I believe that the term carbomer is no longer unfamiliar to everyone. It is a polyacrylic acid cross-linked polymer, which is mainly used for the thickening of daily chemical products. It can achieve the effect at very low concentration. Carbomer is usually used to increase the viscosity of cosmetics such as shampoo, shower gel, detergent, including some creams, and most daily chemical products contain this ingredient.   This buddy has a big fire in 2020, the reason is very simple, it is an important ingredient of daily chemical products, but because of its unique "personality" was recruited for use in hand sanitizer. The arrival of the new crown demon in 2020 makes everyone be cautious and pay attention to health and disinfection. Otherwise, they will be wiped out by the new crown demon if they are not careful. This makes disposable hand sanitizers containing carbomer have been favored by everyone. During this period, no-wash disinfectant hand sanitizers in major shopping malls were even sold out.   In such a hot situation, many people are guilty of carbomer. In the period when money is out of stock, many manufacturers have lost their conscience with other products posing as carbomer and selling them at high prices. Many customers are deceived for these reasons. The so-called ten years of being bitten by a snake are afraid of well ropes. They can't believe it when they see the price of carbomer at 100 yuan/kg. The promotion price of 100 yuan/kg is real!   Although Desheng already has a number of stable customers in the international market, it hopes to expand its market share. This discount is also Desheng giving customers more benefits. The preferential price period is one month, from October 25, 2020 to November 25, 2020. Don't miss it when time is limited. Speaking of this, there will definitely be people who question whether the price is so cheap or the quality is not good. The old saying goes that you get what you pay for. This time, Desheng broke this old saying and achieved price concessions and quality assurance. Desheng was established in 2005 and specializes in polyacrylic acid material products. So far, it has developed three generations of serum separation gels for different purposes and carbomer products for different purposes. Secondly, Desheng is the manufacturer of Carbomer, so all the indicators of its products meet the requirements of international brands, and it can also support individual index customization. Compared with other products, it can be found that Desun Carbomer powder is whiter and has better bulkiness, and the dissolved gel has higher transparency than other products. In addition, Desheng has perfect after-sales service, Desheng promises that any quality problems can be returned and replaced. You can apply for a free sample trial, so you can try it first and then buy it.   This event is aimed at Carbomer 940 and 980, which can be purchased in small quantities or signed annual contracts to enjoy ultra-low discounts. The good quality of Desun Carbomer products is supported by one-stop procurement service. Whether it is direct ordering by customers or procurement by traders, choosing Desun is the right choice.

2020 has entered the last quarter, and the research and development of the new crown vaccine is in full swing around the world, but before the large-scale vaccination of the vaccine, the nucleic acid detection of the virus is still a vital part of the anti-epidemic process. Therefore, Virus transport media and virus sampling tubes are still in huge demand. Which one is better, inactivated or activated?   According to different detection conditions and requirements, the Virus transport media is divided into two types: inactivated and activated. Among them, the inactivated type of Virus transport media contains high concentration of lysis salt, which can quickly lyse and inactivate the virus contained in the sample, but retain the viral nucleic acid; activated type will not inactivate the virus, but can be more completely retained The protein coat and nucleic acid of the virus. Therefore, both types of virus preservation solutions can be used for nucleic acid detection.   Virus transport media for nucleic acid detection After the first round of national nucleic acid testing in China, it can be said that we have the world's leading level in terms of the efficiency and accuracy of viral nucleic acid testing. It also has mature technology and rich experience in the pre-processing of nucleic acid detection such as Virus transport media and virus sampling. So based on the experience of this period of time, is there a better choice among the two Virus transport media ?   As far as the current situation is concerned, it should be that two types of Virus transport media will co-exist, each of which has its own advantages. The inactivated virus preservation solution will inactivate the virus, so that it will not cause secondary infection, but this does not mean that the activated virus is likely to cause infection, because the mature technology, rich experience and strict control can completely eliminate The actual result of the secondary infection in the sampling process was that there was no virus transmission caused by the virus sampling tube.   Similarly, the virus retained by the activated Virus transport media is more complete and the detection is more accurate. It does not mean that the detection of the inactivated type is not accurate enough. In fact, through the improvement of detection technology and sophisticated instruments, the detection result is completely accurate and the detection is more accurate. The viable storage solution has lysed the virus protein, and the nucleic acid can be directly extracted for PCR experiment in the future.   Therefore, activated and inactivated Virus transport media should coexist for a long time. For manufacturers, only one type of preservation solution is more conducive to management and cost control than two types. Desheng still provides inactivated and activated versions. There are two types of active types, which are more convenient for the application enterprises to choose according to their requirements.

My name is carbomer 980, and there is a number 940 that is my brother, the title of Kabo elf, after all, the status is noble, although many people do not know it. I admit that although I am a fat white man, I don't want to be black. The beauty and health of humans depend on us.   I pride myself on my noble status. This is not completely unfounded. We are all high-molecular polymers with a relative molecular weight of 40,000 to 100,000 or even higher, which is not found in nature. Although I am a fat man in appearance, it is just puffiness. I can say that it is a light body and a body weight of only one-fifth of water, which means that a cube is only 200 kilograms. Of course, because it is relatively fluffy, the proportion will increase significantly under heavy pressure, and it can increase by five or six times, and that is a veritable 200 catty fat man.   Regardless of appearance, my capo elf's skills are still very powerful. Although not many people know me, I participate in the cosmetics, creams, lotions, shampoos and other products that people use every day, as well as some medicines. For example, carbomer eye drops and carbomer medicinal gel also contain me, and we are also well-known in the pharmacopoeia.   In this year when the new crown virus is raging around the world, I, Comrade Kabo, is also struggling on the front line of the fight against the epidemic. One of the important ingredients in hand sanitizers is carbomer. We have super water absorption performance, thickening performance, gel performance, etc., not only in water, but also in alcohol or other alcohols, Carbo also has good gel suspension performance. We only need 0.5%-2.0% of the usage amount to form a gel, no matter whether it is the amount of water or the amount of alcohol.   Having said that, Comrade I Capo is definitely a good citizen who practices the core values ​​of socialism and abides by the law. Sadly, he has not been wronged! For example, some people say: Carbo is extracted from pure natural plants and contains Chinese herbal medicines. This is too much. It is said that it is a polymer, a synthetic material! Others say that Carbo can beautify skin, whiten and sunscreen. This is not right. Like water, whether it is used in skin care products or as a medicinal adjuvant, it is just a carrier and a medium. Others say that I can cause cancer. This is even worse. The carcinogen is benzene. Because it is made of petrochemical raw materials, petroleum contains benzene and needs to be removed. The pharmacopoeia requires benzene content and heavy metal content. Don't buy fake and shoddy products.   My self-introduction comes to an end here. The new crown virus is unrelenting and trying to make a comeback. I hope everyone will not take it lightly, gather less and eat more vegetables, and products that will not get sick are sold!

10 mM Tris, 0.1% sodium azide, pH 8.0±0.1   CEA and PSA dilution buffer, specially designed for the preparation of certain CEA, PSA and non-complex PSA. But when other buffer salts are not needed, it is a suitable Tris buffer.   ingredient   Tris base: 1.210 g/L Sodium azide: 1.0 g/L 5M HCl: as required 5M NaOH: as required Distilled water (dH2O)   Instructions for use   1. Use cold dH2O to dissolve sodium trichloride and sodium azide about 90% of the final volume.   2. Add cold dH2O to obtain the final volume.   3. Adjust the pH to 8.0±0.1 with 5M HCl or 5M NaOH as needed. Filter as needed.   10 mM Tris, 150 mM sodium chloride, 0.1% sodium azide, pH 8.0±0.1   Ferritin dilution buffer is specially designed for liver ferritin and spleen ferritin. It is suitable for Tris buffer used when other buffer salts are needed.   ingredient   Tris HCl: 1.296 g / L   Tris base: 0.215 g/L   Sodium chloride: 8.766 g/L   Sodium azide: 1.0 g/L   5M HCl: as required   5M NaOH: as required   Distilled water (dH2O)   Instructions for use   1. Use cold dH2O to dissolve Tris-HCl, Tris-Base, sodium chloride and sodium azide about 90% of the final volume.   2. Adjust the pH to 8.0±0.1 with 5M HCl or 5M NaOH as needed. To   3. Add cold dH2O to obtain the final volume.   4. Check the pH value and adjust the pH to 8.0±0.1 with 5M HCl or 5M NaOH as needed.   20 mM Tris, 1 mM EDTA, 50% glycerol, pH 8.5±0.1   Myoglobin dilution buffer is specially designed for cardiac myoglobin and skeletal muscle myoglobin. When EDTA and glycerol provide extra stability, it is suitable for Tris buffer.   ingredient   Tris HCl: 1.950 g/L Tris base: 0.920 g/L EDTA, four Sodium: 0.416 g/L Glycerin: 500 ml/L 5M HCl: as required 5M NaOH: as required Distilled water (dH2O)   Instructions for use   1. Dissolve about 45% of the final volume of Tris-HCl, Tris-Base and EDTA with cold dH2O.   2. Adjust the pH to 8.5±0.1 with 5M HCl or 5M NaOH as needed. To   3. Add cold dH2O to obtain about 50% of the volume.   4. Add cold glycerin to obtain final volume, mix well and cool.   5. Check the pH value and adjust the pH to 8.5±0.1 with 5M HCl or 5M NaOH as needed.   20 mM Tris, 150 mM sodium chloride, pH 7.4±0.1   ACT dilution buffer, specially designed for a1 anti-chymotrypsin. This solution is similar to the ferritin dilution buffer, but with a higher concentration of Tris and a slightly lower pH. To   ingredient   Tris base: 2.420 g/L   Sodium chloride: 8.766 g/L   5M HCl: as required   5M NaOH: as required   Distilled water (dH2O)   Instructions for use   1. Dissolve sodium trichloride and sodium chloride with cold dH2O, about 90% of the final volume.   2. Adjust the pH to 7.4±0.1 with 5M HCl as needed.   3. Add cold dH2O to obtain final volume and cool.   4. Check the pH value and adjust the pH to 7.4±0.1 with 5M HCl or 5M NaOH as needed.   5. Use a 0.45 µm filter for filtration.   20 mM Tris, 280 mM sodium chloride, 5 mM calcium chloride, 0.1% sodium azide, pH 8.0±0.1   CRP dilution buffer, specially designed for highly purified C-reactive protein, is suitable for situations that require higher concentrations of Tris, buffer salts and calcium chloride.   ingredient   Tris base: 2.423 g/L Sodium chloride: 16.363 g / L Calcium chloride: 0.735 g/L Sodium azide: 1.0 g/L 5M HCl: about 3 ml / L 5M NaOH: as required Distilled water (dH2O)   Instructions for use   1. Dissolve sodium trichloride and sodium chloride with cold dH2O, about 90% of the final volume. 2. Adjust the pH to 8.0±0.1 with 5M HCl as needed. 3. Add cold dH2O to obtain final volume and cool. 4. Check the pH value, adjust to pH 8.0±0.1 with 5M HCl or 5M NaOH as needed, and then add sodium azide. 5. Add calcium chloride to completely dissolve it. (Important: The pH cannot be adjusted after adding calcium chloride.) 5. Use a 0.2 µm filter for filtration.

Tris is a zwitterionic buffer, often used in biology and scientific research experiments, of course, there are also many directly used in medicine and industry. So what are the specific uses of the different buffers of tris, how to use the buffers of tris, and understand the characteristics of tris and different biotechnologies, such as electrophoresis or chromatography.   Basic information of TRIS base: CAS number: 77-86-1 Molecular weight: 121.14 g / mol Formula: C 4 H 11 NO 3 Available pH range: 7.2-9.0 pKa (25°C): 8.06 Maximum wavelength (λmax)λ: 260 nm Amax: 0.10: λ: 280 nm Amax: 0.08   What is TRIS recommended for? lCommonly used in gel electrophoresis buffer (TAE or TBE) lUsually used to prepare Laemmli buffer (used for denaturation and loading of protein samples in SDS-PAGE) lAnion exchange chromatography lUsed to evaluate bacterial endotoxin lFor capillary electrochromatography (CEC), due to its low ion mobility lUsed for metal complexation l Can be used to correct acidosis What issues should be considered before choosing TRIS for research? uNot very effective below pH 7.5 uIt has potentially reactive primary amines and can usually be used as an inhibitor uIt tends to permeate the membrane, so it is not suitable for most cell culture work uToxic to many mammalian cells uIt is not suitable for bicinchoninic acid (BCA) analysis uIt may interfere with the Bradford dye binding method for protein determination uChelated calcium and other essential metals are well known uThe pH of the solution containing Tris is very dependent on temperature, so it is very important to prepare Tris buffer at the temperature of use uIt may interact with the bicarbonate/CO2 buffer system According to the use of different industries, the considerations are different, and the requirements for the level of tris will also be different, the purity and some other indicators will also be relatively different, and these factors often require buyers and manufacturers to be able to deepen Communication can be better provided.   Tips about TRIS: ØHigh solubility in organic solvents ØIt reacts with various enzymes and inhibits several enzymes Ø It can inactivate the popular RNase inhibitor DEPC

NSP-DMAE-NHS Introduction   Full name NSP-DMAE-NHS CAS 194357-64-7 Molecular formula C30H26N2O9S Molecular weight 590.6 Exterior Yellow solid/powder purity ≥98% package 10mg/bottle, 50mg/bottle, 100mg/bottle, 1g/bottle   Compared with other luminescent reagents, the use of acridine esters is really not that much. This directly leads to the fact that there are only a handful of manufacturers producing acridine esters, and the quality is decent. A few. Therefore, it is difficult for many buyers to really find acridine ester manufacturers, and they are often dealers who have gone through a few hands, and the price will be much more expensive. Desheng is a professional manufacturer of acridinium esters. It has a professional R&D and production team. The product quality is guaranteed and the price is relatively more favorable.   The price will also fluctuate according to the amount of purchase. For example, the smallest package of Desheng is 10mg/bottle, and the price is around 160 yuan/mg. The larger the purchase, the cheaper the price.                                                                            Whether it is NSP-DMAE-NHS or other acridine esters, most of them are used in other technologies such as chemiluminescence, high performance liquid chromatography, sensor technology and flow injection technology. Because of the direct emission mode, the analysis speed is relatively fast. , The equipment needed is simpler than other luminescent reagents, and the sensitivity is also very high. It is widely used in the fields of chemical food safety, biomedicine, environmental testing and so on.   Features of NSP-DMAE-NHS   Conventional acridine esters have high quantum yields, higher luminous efficiency than other luminescent reagents, less interference, low background, easier to couple, simple labeling and good stability, and the effect of labeling on luminescence performance very small. This also makes NSP-DMAE-NHS widely used.   NSP-DMAE-NHS packaging and storage   In many cases, the acridinium ester is more troublesome to pack, and it will cause some losses when it is packed, so the smaller the pack, the higher the price. The bottles of luminescent reagents are generally impermeable bottles. For example, this NSP-DMAE-NHS acridinium ester product is also sealed in brown bottles. When transporting, Desheng uses ice packs. Regular storage is It is valid for two years at 2-8℃.

PCR laboratory is also called gene amplification laboratory. Real-time fluorescent quantitative PCR technology is a leap forward in DNA quantitative technology. It is used to amplify specific DNA fragments, which can be regarded as special DNA replication in vitro. Through the DNA gene tracking system, the virus content in the patient's body can be quickly grasped with an accuracy of nanometer level. The fluorescent PCR method is the carrier that realizes this technology. Through the method of amplifying the genes contained in the virus, it can detect whether some infected persons with low virus content contain specific viruses.   The PCR laboratory is actually extremely powerful. Qualitative analysis and quantitative detection are its two biggest application directions. The application in the detection of new crown nucleic acid is particularly prominent. Next, Desheng will introduce to you the application of PCR in the detection of pathogens. I hope that through these examples, you can better understand what the nucleic acid detection process is. https://www.vacutaineradditives.com/ Example: Novel coronavirus nucleic acid detection. Methodology: fluorescence PCR method. Uses: Used for in vitro qualitative detection of suspected cases of pneumonia, suspected clusters of patients with new coronavirus infection, and other patients who need to diagnose or identify new coronavirus infection. Detection gene: new coronavirus (2019-nCoV) ORFlab and N gene, E gene. Specimen types: upper respiratory tract specimens: throat swabs, nasal swabs; lower respiratory tract specimens: respiratory tract extracts, alveolar lavage fluid samples, lung tissue biopsy specimens.   Detection process: (1) Biosafety protection before nucleic acid detection (personal three-level protective wear by staff) → (2) Inactivation of samples (inactivation at 56°C for 30 minutes) → (3) Nucleic acid extraction of samples (manual, nucleic acid extractor) )→(4) PCR system preparation and template loading→(5) On-board testing and result analysis→(6) Disinfection after testing.   Other pathogen detection: HIV, hepatitis A, B, C, HPV, lung branches, lung coat, Epstein-Barr virus, adenovirus, cytovirus, A and B stream, Ebola virus, group B streptococcus. Hospitals and departments suitable for development: children's hospitals, maternity and children's clinics, pediatric clinics, third parties, hospitals of various levels in need   The advent of PCR technology enables rapid and convenient pathogen detection. The PCR detection method has the advantages of high sensitivity, high specificity, quickness, and low sample requirements. Therefore, it is widely recognized by clinicians and has been widely used in clinical diagnosis in hospitals and the diagnosis of poultry disease in various epidemic prevention and detection departments.

With people's emphasis on health and increasingly stringent environmental regulations, water-dispersible polyisocyanates have shown importance in various application fields in recent years. At present, water-dispersed polyisocyanates are mainly used as crosslinking components to prepare water-dilutable two-component polyurethane coatings (2K-PUR coatings), or as additives for water dispersion adhesives, in textile finishing or formaldehyde-free Used in textile printing inks to crosslink water dispersions.     At present, water-dispersible polyisocyanates in most application fields are non-ionic hydrophilic modification through polyethers. Although this type of hydrophilic modified polyisocyanate has been widely recognized in the market in most applications, it also has certain drawbacks, such as its high viscosity, which requires considerable shear force to be uniformly distributed during the construction process. Introduced into the aqueous medium. In order to avoid the above shortcomings, researchers have tried to prepare water-dispersible polyisocyanates by adding ionic groups for hydrophilic modification.   In the reported literature or patents, ion-modified groups include carboxyl, sulfuric acid, hydroxyl, hydroxysulfonic acid, etc., but there are still certain defects, such as carboxyl-modified polymers are prone to gelation and hydroxyl The color of sulfonic acid modified products is obviously yellow. Later, Bayer reported in its patents 3-(cyclohexylamino)-propanesulfonic acid (CAPS) modified polyisocyanates. Research has found that CAPS-modified polyisocyanates can be finely dispersed in water and the product is stable in storage.   Below, the editor of Desheng briefly introduces the preparation method of this type of polyisocyanate. 950 g of polyisocyanate, 50 g of CAPS, 29 g of dimethylcyclohexylamine and 257 g of 1-methoxyprop-2-yl acetate were stirred for 5 hours at 80 degrees Celsius under dry nitrogen, wherein the polyisocyanate was based on hexamethylene Diisocyanate (HDI) and contains isocyanurate groups, NCO content is 21.7%, average NCO functionality is 3.5, monomer HDI content is 0.1% and viscosity is 3000 mPas. After cooling to room temperature, a colorless and transparent polyisocyanate solution can be obtained.   Believe that you will see here that you will conclude that CAPS-based modified polyisocyanates show certain advantages over other ionic or non-ionic modified products. In addition, as environmental protection requirements become more stringent and people’s awareness of environmental protection increases, the market acceptance of water-based coatings will gradually surpass that of solvent-based coatings as people implement a healthy and environmentally friendly life.   The CAPS buffer produced by Desheng Company has not only been widely used in the new coating market, it is also popular in the biochemical field, because it is also a biological buffer, which is very widely used in biochemical diagnostic kits, DNA/RNA In the extraction kit and PCR diagnostic kit, if you have any questions or needs, please go to the official website to consult customer service staff.