China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Tris is a zwitterionic buffer, often used in biology and scientific research experiments, of course, there are also many directly used in medicine and industry. So what are the specific uses of the different buffers of tris, how to use the buffers of tris, and understand the characteristics of tris and different biotechnologies, such as electrophoresis or chromatography.   Basic information of TRIS base: CAS number: 77-86-1 Molecular weight: 121.14 g / mol Formula: C 4 H 11 NO 3 Available pH range: 7.2-9.0 pKa (25°C): 8.06 Maximum wavelength (λmax)λ: 260 nm Amax: 0.10: λ: 280 nm Amax: 0.08   What is TRIS recommended for? lCommonly used in gel electrophoresis buffer (TAE or TBE) lUsually used to prepare Laemmli buffer (used for denaturation and loading of protein samples in SDS-PAGE) lAnion exchange chromatography lUsed to evaluate bacterial endotoxin lFor capillary electrochromatography (CEC), due to its low ion mobility lUsed for metal complexation l Can be used to correct acidosis What issues should be considered before choosing TRIS for research? uNot very effective below pH 7.5 uIt has potentially reactive primary amines and can usually be used as an inhibitor uIt tends to permeate the membrane, so it is not suitable for most cell culture work uToxic to many mammalian cells uIt is not suitable for bicinchoninic acid (BCA) analysis uIt may interfere with the Bradford dye binding method for protein determination uChelated calcium and other essential metals are well known uThe pH of the solution containing Tris is very dependent on temperature, so it is very important to prepare Tris buffer at the temperature of use uIt may interact with the bicarbonate/CO2 buffer system According to the use of different industries, the considerations are different, and the requirements for the level of tris will also be different, the purity and some other indicators will also be relatively different, and these factors often require buyers and manufacturers to be able to deepen Communication can be better provided.   Tips about TRIS: ØHigh solubility in organic solvents ØIt reacts with various enzymes and inhibits several enzymes Ø It can inactivate the popular RNase inhibitor DEPC

NSP-DMAE-NHS Introduction   Full name NSP-DMAE-NHS CAS 194357-64-7 Molecular formula C30H26N2O9S Molecular weight 590.6 Exterior Yellow solid/powder purity ≥98% package 10mg/bottle, 50mg/bottle, 100mg/bottle, 1g/bottle   Compared with other luminescent reagents, the use of acridine esters is really not that much. This directly leads to the fact that there are only a handful of manufacturers producing acridine esters, and the quality is decent. A few. Therefore, it is difficult for many buyers to really find acridine ester manufacturers, and they are often dealers who have gone through a few hands, and the price will be much more expensive. Desheng is a professional manufacturer of acridinium esters. It has a professional R&D and production team. The product quality is guaranteed and the price is relatively more favorable.   The price will also fluctuate according to the amount of purchase. For example, the smallest package of Desheng is 10mg/bottle, and the price is around 160 yuan/mg. The larger the purchase, the cheaper the price.                                                                            Whether it is NSP-DMAE-NHS or other acridine esters, most of them are used in other technologies such as chemiluminescence, high performance liquid chromatography, sensor technology and flow injection technology. Because of the direct emission mode, the analysis speed is relatively fast. , The equipment needed is simpler than other luminescent reagents, and the sensitivity is also very high. It is widely used in the fields of chemical food safety, biomedicine, environmental testing and so on.   Features of NSP-DMAE-NHS   Conventional acridine esters have high quantum yields, higher luminous efficiency than other luminescent reagents, less interference, low background, easier to couple, simple labeling and good stability, and the effect of labeling on luminescence performance very small. This also makes NSP-DMAE-NHS widely used.   NSP-DMAE-NHS packaging and storage   In many cases, the acridinium ester is more troublesome to pack, and it will cause some losses when it is packed, so the smaller the pack, the higher the price. The bottles of luminescent reagents are generally impermeable bottles. For example, this NSP-DMAE-NHS acridinium ester product is also sealed in brown bottles. When transporting, Desheng uses ice packs. Regular storage is It is valid for two years at 2-8℃.

PCR laboratory is also called gene amplification laboratory. Real-time fluorescent quantitative PCR technology is a leap forward in DNA quantitative technology. It is used to amplify specific DNA fragments, which can be regarded as special DNA replication in vitro. Through the DNA gene tracking system, the virus content in the patient's body can be quickly grasped with an accuracy of nanometer level. The fluorescent PCR method is the carrier that realizes this technology. Through the method of amplifying the genes contained in the virus, it can detect whether some infected persons with low virus content contain specific viruses.   The PCR laboratory is actually extremely powerful. Qualitative analysis and quantitative detection are its two biggest application directions. The application in the detection of new crown nucleic acid is particularly prominent. Next, Desheng will introduce to you the application of PCR in the detection of pathogens. I hope that through these examples, you can better understand what the nucleic acid detection process is. https://www.vacutaineradditives.com/ Example: Novel coronavirus nucleic acid detection. Methodology: fluorescence PCR method. Uses: Used for in vitro qualitative detection of suspected cases of pneumonia, suspected clusters of patients with new coronavirus infection, and other patients who need to diagnose or identify new coronavirus infection. Detection gene: new coronavirus (2019-nCoV) ORFlab and N gene, E gene. Specimen types: upper respiratory tract specimens: throat swabs, nasal swabs; lower respiratory tract specimens: respiratory tract extracts, alveolar lavage fluid samples, lung tissue biopsy specimens.   Detection process: (1) Biosafety protection before nucleic acid detection (personal three-level protective wear by staff) → (2) Inactivation of samples (inactivation at 56°C for 30 minutes) → (3) Nucleic acid extraction of samples (manual, nucleic acid extractor) )→(4) PCR system preparation and template loading→(5) On-board testing and result analysis→(6) Disinfection after testing.   Other pathogen detection: HIV, hepatitis A, B, C, HPV, lung branches, lung coat, Epstein-Barr virus, adenovirus, cytovirus, A and B stream, Ebola virus, group B streptococcus. Hospitals and departments suitable for development: children's hospitals, maternity and children's clinics, pediatric clinics, third parties, hospitals of various levels in need   The advent of PCR technology enables rapid and convenient pathogen detection. The PCR detection method has the advantages of high sensitivity, high specificity, quickness, and low sample requirements. Therefore, it is widely recognized by clinicians and has been widely used in clinical diagnosis in hospitals and the diagnosis of poultry disease in various epidemic prevention and detection departments.

With people's emphasis on health and increasingly stringent environmental regulations, water-dispersible polyisocyanates have shown importance in various application fields in recent years. At present, water-dispersed polyisocyanates are mainly used as crosslinking components to prepare water-dilutable two-component polyurethane coatings (2K-PUR coatings), or as additives for water dispersion adhesives, in textile finishing or formaldehyde-free Used in textile printing inks to crosslink water dispersions.     At present, water-dispersible polyisocyanates in most application fields are non-ionic hydrophilic modification through polyethers. Although this type of hydrophilic modified polyisocyanate has been widely recognized in the market in most applications, it also has certain drawbacks, such as its high viscosity, which requires considerable shear force to be uniformly distributed during the construction process. Introduced into the aqueous medium. In order to avoid the above shortcomings, researchers have tried to prepare water-dispersible polyisocyanates by adding ionic groups for hydrophilic modification.   In the reported literature or patents, ion-modified groups include carboxyl, sulfuric acid, hydroxyl, hydroxysulfonic acid, etc., but there are still certain defects, such as carboxyl-modified polymers are prone to gelation and hydroxyl The color of sulfonic acid modified products is obviously yellow. Later, Bayer reported in its patents 3-(cyclohexylamino)-propanesulfonic acid (CAPS) modified polyisocyanates. Research has found that CAPS-modified polyisocyanates can be finely dispersed in water and the product is stable in storage.   Below, the editor of Desheng briefly introduces the preparation method of this type of polyisocyanate. 950 g of polyisocyanate, 50 g of CAPS, 29 g of dimethylcyclohexylamine and 257 g of 1-methoxyprop-2-yl acetate were stirred for 5 hours at 80 degrees Celsius under dry nitrogen, wherein the polyisocyanate was based on hexamethylene Diisocyanate (HDI) and contains isocyanurate groups, NCO content is 21.7%, average NCO functionality is 3.5, monomer HDI content is 0.1% and viscosity is 3000 mPas. After cooling to room temperature, a colorless and transparent polyisocyanate solution can be obtained.   Believe that you will see here that you will conclude that CAPS-based modified polyisocyanates show certain advantages over other ionic or non-ionic modified products. In addition, as environmental protection requirements become more stringent and people’s awareness of environmental protection increases, the market acceptance of water-based coatings will gradually surpass that of solvent-based coatings as people implement a healthy and environmentally friendly life.   The CAPS buffer produced by Desheng Company has not only been widely used in the new coating market, it is also popular in the biochemical field, because it is also a biological buffer, which is very widely used in biochemical diagnostic kits, DNA/RNA In the extraction kit and PCR diagnostic kit, if you have any questions or needs, please go to the official website to consult customer service staff.

Specific uses of cosmetics   Desheng produces high-quality Good's Buffer, and can customize product packaging and some indicators according to customer needs. Many buffers in Good's Buffe are used in cosmetics, such as toners, essences, lotions, eye creams and facial masks. Here are three kinds of Good's Buffer commonly used in cosmetics, as follows:   1) HEPES buffer CAS Number: 7365-45-9 Useful pH range: 6.8-8.2 Uses and precautions: ◆Acidity regulator or pH control agent. ◆Identified that the effective concentration of HEPES can provide exfoliation. ◆Promote the transdermal absorption of various functional ingredients in cosmetics. 2) TRIS CAS number: 77-86-1 Useful pH range: 7.2-9.0 Uses and precautions: ◆Non-ionic surfactant-amine. ◆Acidity regulator or pH control agent. ◆Excellent cleaning ability and emulsifying ability. 3) BIS-TRIS CAS Number: 5625-37-6 Useful pH range: 6.1-7.5 Uses and precautions: ◆Non-ionic surfactant-amine. ◆Acidity regulator or pH control agent. ◆Excellent cleaning, dispersing, emulsifying and antistatic ability. In fact, there are many buffers that are suitable for use in cosmetics and play different roles, but the prerequisite is good quality, because after all, the use of cosmetics is still on the human body. Most buffers are used in cosmetics to adjust the pH of their raw materials or solvents. Of course, there are also surfactants such as the above-mentioned surfactants, and some of them have exfoliating effects to promote skin absorption. In terms of dosage, different cosmetics increase the amount differently.

We generally know that luminol is a chemiluminescent reagent, which is generally used for blood testing. It is a yellow crystal or beige powder at room temperature. It is a strong acid and has a certain irritating effect on eyes, skin and respiratory tract. Under alkaline conditions, it can be oxidized by peroxides and emit light at the same time. The redox reaction between luminol and peroxide requires a catalyst. This catalyst is generally a multivalent metal ion, peroxidase such as iron and horseradish. Peroxidase, etc., this method is often used to detect the content of peroxides, heavy metals, peroxidase, and the derived free radicals, for toxicant analysis and analysis methods based on peroxidase and glucose oxidase .                                                                               Luminol Luminous Picture It is our incomplete understanding of it that leads to many questions about him in many cases. 1. When luminol detects blood, can it distinguish between human and animal blood? Answer: No, because luminol detects blood mainly using the catalysis of iron in hemoglobin, and animal hemoglobin is not much different from humans, so luminol can only detect blood and cannot distinguish what kind of blood it is. If you accidentally mix human blood with blood from other animals, it is impossible to determine whether it is human blood just with luminol. But luminol has a good advantage that it does not affect DNA changes, so as long as we can detect blood, we can distinguish between human blood and other blood in DNA comparison.   2. Will the blood be washed with detergent affect the detection of luminol? Answer: If the criminal suspect takes some measures after committing the crime, such as cleaning the scene with hypochlorous acid bleach, as long as he sprays luminol on the crime scene that has been washed with hypochlorous acid, it will glow everywhere, which will interfere with blood stains. Detection. However, although both luminol and hypochlorous acid can make luminol emit light, the two luminescence phenomena are different. The luminescence caused by bleach flashes quickly, while the luminescence caused by blood stains appears gradually.   3. Can we continue to use luminol after one reaction? Answer: The luminescence principle of luminol is because hemoglobin contains iron, and iron can catalyze the decomposition of hydrogen peroxide, which turns hydrogen peroxide into water and monooxygen, and monooxygen oxidizes luminol to make it glow. When we use luminol, it will not change and oxidize the iron ions in the blood, and iron ions can continue to play a catalytic role as a catalyst. Therefore, the light-emitting process can continue to react. So the second use of luminol can still see the light.   4. Is it toxic if it accidentally sticks to the hands when using luminol? Answer: The skin absorbs luminol very poorly. Even if luminol enters the body through the skin or other means, it will not deposit in the body or cause chronic toxicity. Moreover, there has been no report of luminol poisoning caused by improper protection. It can be inferred from the above that even if luminol easily enters the blood through the mucosa of the gastrointestinal tract, it will be quickly excreted from the urine. The chance of luminol passing through the skin or depositing in the body is very small, thus causing chronic poisoning. The possibility is very small.   The above are a few common questions we have sorted out through customer feedback, and hope that we can really help you in the follow-up use. Desheng Technology has more than ten years of experience in the field of chemiluminescence reagents. In addition to luminol, we also have acridine esters and their derivatives, which have left a good reputation for customers. We will continue to do our products with heart.

A pandemic caused carbomer...Carbomer raw materials are mainly used as daily chemical products, skin care creams, lotions, etc., as well as the gel matrix in various medicinal excipients. It has efficient thickening, gelation, and suspension. Performance etc. Because of its good water absorption and suspending properties, it has become one of the main raw materials in the disposable hand sanitizing gel that is widely used during the epidemic. This hand sanitizer is not liquid and will not flow randomly, and it has disinfection, Features such as no-washing are very suitable for quick disinfection of hands.                                                                        Desheng Carbomer 940 powder    Due to its indispensable position in the daily chemical, pharmaceutical and other industries, high-quality carbomer raw materials have always been urgently demanded products in the market. The special circumstances affected by the epidemic this year have made Carbomer a popular raw material this year. There are countless companies that provide carbomer raw materials. It can be said that buyers are confused. What kind of carbomer raw materials company is more worth choosing?   1. Product quality is reliable   Desheng conducted a small trial production of carbomer in October 2019. At the beginning of 2020, the company invested a lot of capital, manpower and material resources. After the R&D staff worked overtime, the final test of carbomer was completed after several tests. The carbomer raw materials produced are up to standard in all indexes, which are comparable to international brands. Its transparency is better than that of well-known brands.   2. Good sales service   Desheng provides one-stop procurement services. After purchasing carbomer raw materials, customers may still encounter problems such as dissolution and agglomeration, how to use carbomer powder to make gels, etc., which require special guidance, which requires the supply of selected raw materials The supplier can provide the corresponding after-sales service. From this point of view, it is more appropriate for customers to choose some carbomer raw materials companies with good market reputation and more professional sales services.   Third, the product is cost-effective   When choosing what kind of carbomer raw material company to cooperate with, the quotation provided by the relevant company is also an important reference standard, especially when the demand for carbomer is large, the corresponding raw material purchase volume will also double. If the supplier can provide a more competitive quotation, there will definitely be more room for maneuverability in terms of cost control. Desheng is the manufacturer of Carbomer, direct sales of raw materials. So compared with other traders, the product cost performance will be higher.   I believe that after reading the above analysis, Desheng will be your worthy carbomer supplier. Now the company has begun to implement mass production, with an average daily output of 1t, and has rich experience in exporting. If necessary, please call for detailed inquiry.

National Day has passed, and 2020 is entering the final quarter, but the global epidemic has not planned to end over time. Although the country has achieved victory in the fight against the epidemic, in order to prevent a second outbreak, all walks of life cannot be taken lightly. In this situation, what should I pay attention to when purchasing reagents that are already cumbersome? The procurement of biochemical reagents, especially in vitro diagnostic reagents, is different from many bulk commodities. The method of “being afraid of comparing goods without knowing the goods” is usually not applicable. If it is a large quantity of chemical raw materials, the purchase period is usually large and the amount is relatively easy to handle. If it is a small amount of reagents that are purchased frequently, and there are many types, it will be a headache for purchasers. But there are still some ways to improve this situation. Procurement of in vitro diagnostic reagents 1. Learn more about professional knowledge As the saying goes, a worker must first sharpen his tools if he wants to do his job well, and mastering a certain degree of professional knowledge can often have a multiplier effect for purchasing. Like in vitro diagnostic reagents, there are many things that cannot be judged by name, and sometimes even the same CAS number may not be the product to be purchased. Different reagents have different grades, concentrations, purity, etc. in different applications. 2. Make good use of network information In fact, before the epidemic, current purchasers would use the Internet to purchase various reagent products. After industry exhibitions have decreased and travel is not convenient, it has become more common to purchase via the Internet. However, online procurement also has its drawbacks. A lot of information is not authentic enough, and it is inevitable that you will be deceived. You must be careful about this. Moreover, due to the situation of the epidemic, illegal incidents of dishonesty on the Internet have occurred from time to time. 3. Make records and preparations Due to the unpopularity of in-vitro diagnostic reagents and the variety of names, some products are very troublesome to find on the Internet, which often delays the procurement time to the nearby delivery time. At this time, purchasers are often more anxious, and it is easier at this time They are fooled into buying fake products, or products of poor quality, and even directly defrauded of money. Therefore, it is necessary to record and prepare related reagent products at ordinary times to avoid panic when looking for goods temporarily. Due to the impact of the epidemic, in order to seek new opportunities, many companies may also purchase some diagnostic reagents that were not familiar in the past. Therefore, it is necessary to communicate and learn more with technicians when purchasing. At the same time, you can also consult professionals like Desheng for the corresponding products. Reagent manufacturing enterprises.

The full name of Tris base in Chinese is Tris, CAS77-86-1, which is an important biological buffer and a reference material for acid titration, and has an extremely wide range of social applications. At present, there is a lot of information on the application of Tris in the fields of biochemistry and medicine, but in fact, Tris is also widely used in various researches. This article will summarize the uses of Tris.   Biological buffer:   The most well-known application of Tris is as a solvent for nucleic acids and proteins and as a biological buffer. Tris-HCl, NaCl, tween20 these three substances can form a TBST buffer, which is a commonly used washing buffer in Western Blotting; Tris-HCl can be made into TE buffer by adding EDTA to it, which can be used for DNA stability and Storage; Replace the acid solution that adjusts the pH value with acetic acid to obtain TAE buffer, and change to boric acid to obtain TBE buffer. These two buffers are often used in nucleic acid electrophoresis experiments.   Tris itself is alkaline. Only the Tris-HCl buffer system can be used to prepare buffers with a pH range from acidic to alkaline. The method is relatively simple, and it can also be combined with other substances to form a variety of buffer systems; it can interfere with biochemical processes. It is small and does not precipitate with calcium, magnesium ions and heavy metal ions. However, the pH value of Tris buffer is greatly affected by the concentration and temperature of the solution. When diluted ten times, the pH value of the buffer changes more than 0.1. For every degree of temperature rise, the pH value drops by 0.03; at the same time, it easily absorbs CO2 in the air. Therefore, the prepared buffer should be tightly sealed.   Surfactant refers to a substance that can significantly change the interface state of the solution system by adding a small amount, and has a fixed hydrophilic and lipophilic group, which can be aligned on the surface of the solution. The application of surfactants can be divided into civil and industrial applications. Two-thirds of civil surfactants are used in personal protection products, and industrial surfactants are the sum of surfactants used in various industrial fields other than civil surfactants.   The molecular structure of surfactants is amphiphilic: one end is a hydrophilic group, the other end is a hydrophobic group; the hydrophilic group is often a polar group, such as carboxylic acid, sulfonic acid, sulfuric acid, amino or amine group and Its salt, hydroxyl group, amide group, ether bond, etc. can also be used as polar hydrophilic groups; while hydrophobic groups are often non-polar hydrocarbon chains, such as hydrocarbon chains with more than 8 carbon atoms. Tris contains three hydroxyl groups and one amino group, both of which are good hydrophilic groups. Therefore, Tris is also an important intermediate for the synthesis of surfactants.   Vulcanization accelerator is a substance that can promote vulcanization, which can shorten the vulcanization time, lower the vulcanization temperature, reduce the amount of vulcanizing agent and improve the physical and mechanical properties of rubber. The accelerator with Tris as the intermediate is mainly used as the hardener of resin and coating; also used in the ink of printed circuit board, electrical insulating material, resin mold, screen printing coating, mold, adhesive, lining Materials and plastic stabilizers, etc.   In the medical field, the application of Tris is mainly divided into pharmaceutical intermediates and medicine. Tris is an intermediate of fosfomycin tromethamine. Fosfomycin tromethamine is a broad-spectrum antibacterial drug marketed in the late 1980s. Its bioavailability is comparable to that of the commonly used antimicrobial fosfomycin calcium. 3~6 times, more suitable for the treatment of urinary tract infections and other diseases.   Tris is also a drug used to treat blood diseases, and can be used for acute metabolic acidosis and acute respiratory acidosis. As a sodium-free amino buffer base, it combines with H2CO3 in body fluids to generate bicarbonate. Therefore, it can buffer acid and reduce CO2 tension, and has the dual effect of correcting acute respiratory acidosis and metabolic acidosis. Its effect is faster and stronger than sodium bicarbonate. It is especially suitable for patients with heart failure and acidosis. It is also suitable for the rescue of hypothermic anesthesia, shock and cardiac arrest. After organ transplantation, this drug can be used to treat ischemic intracellular acidosis. .   Tris has three equal hydroxyl groups and can be used as a good reducing agent. Some researchers use Tris as a reducing agent to reduce the chloroauric acid solution by ultrasound under alkaline conditions without adding other stabilizers to prepare relatively stable gold nanoparticles, realizing environmentally friendly and biocompatible gold nanoparticles Synthesis of gold nanoparticles. The experimental conditions are mild, the operation is simple and easy, and it is green and pollution-free, low-toxic and low-cost.   Tris is widely used, from laboratory biochemical experiments, to industrialized mass production, to daily necessities, it is indispensable. Speaking of its toxicity, it is a piece of cake compared to some daunting reagents, but the deep erosion has to be prevented. Tris can be harmful to the body through inhalation, ingestion, and skin absorption. Therefore, for field experimenters, wearing gloves and goggles during operation is an effective protective measure, and safe production can be used with confidence.   Desheng is a professional manufacturer of biological buffer raw materials, and Tris is the company's main advantage product. Desheng’s main product biological buffer is a kind of alcohol and ammonia fine chemicals with special characteristics. It does not participate in and does not interfere with the biochemical process. It can be used as a buffer system for life science research to provide a stable pH environment for experiments. Desheng biological buffer Agents are widely used in in vitro diagnostics, biopharmaceuticals, biological cosmetics, coatings and paints and other industries. Due to the high buffering efficiency and quality of the products, there are now dozens of stable customers. Desheng provides one-stop procurement services. If you need it, please click on the official website to consult customer service.

Currently, heparin sodium is mainly derived from the intestinal mucosa of pigs, cattle or sheep. However, heparin steel of bovine and sheep origin is at risk of being infected by related viruses, and the incidence of adverse reactions such as thrombocytopenia and thrombosis syndrome is much greater than that of pig-derived heparin steel. Therefore, in clinical use, people should try to choose porcine heparin sodium. However, in actual production, due to various reasons such as complex sources of production materials, the production raw materials from pigs are often contaminated by animal materials from cattle and sheep. Therefore, the establishment of methods for the identification of ingredients from pigs, sheep, cattle and other animal sources is essential to control the quality of drugs and prevent the contamination of xenogeneic animal-derived ingredients.   The following briefly talk about the detection principle of different species of heparin sodium, the detection principle: heparin sodium is a glycosaminoglycan, which is composed of uronic acid (L. iduronic acid, IdoA; D-glucose Uronic acid (GlcA) and glucosamine (6[.D-glucosamine, GlcN) are a mixture of polysaccharide chains with different chain lengths. Heparinase is used to enzymatically hydrolyze heparin sodium from different species, and its enzymatic hydrolysis products are analyzed and studied; that is, the species source of this product is characterized by the composition ratio of the enzymatic hydrolysis products (disaccharide mixture).   Heparinase is a type of polysaccharide lyase that acts on heparin or heparan sulfate molecules; there are three types of heparinase, namely heparinase I, heparinase II and heparinase III. Since each heparinase has a unique substrate specificity, they cut a specific site in the heparin chain. Heparinase 1 decomposes the Oc(1-4) glycosidic bond between GlcNs(6S) and IdoA(2S), and can cut off the antithrombin III binding pentasaccharide site in the heparin molecule; heparinase II can decompose two The sugar contains 2 or 3 sulfated groups of heparin, so its specificity is not strong; heparinase II1 decomposes the 6-position unsulfated GlcNS or IV. Heparin of Acetyl Glucosamine (GlcNAc) (see Figure 1). The proper mixing of the three heparinases can completely degrade heparin into an unsaturated disaccharide mixture with ultraviolet absorption at 234nm.   The detection of the source of heparin species mainly uses quantitative PCR to detect heparin sodium from different species, but this method is costly. The heparinase enzymatic hydrolysis method can effectively identify heparin samples mixed with sheep-derived crude heparin, and is simple and fast. The only disadvantage is that heparinase will lose the information of the uronic acid epimer during the enzymatic hydrolysis of heparin. Cannot distinguish between glucuronic acid and iduronic acid. In addition, in recent years, there have been methods for separating various disaccharides by capillary electrophoresis. Reversed-phase ion-pair chromatography can also be used to separate heparin disaccharides, and combined with mass spectrometry for quantitative analysis.   Desheng is a professional manufacturer of blood collection reagents and also has a complete detection system. The heparin sodium produced by it is mainly used as an additive for blood collection tubes. Heparin sodium has good anticoagulant effect, potency, and fast delivery.    

Chemiluminescence immunoassay is an in vitro diagnostic reagents method developed after enzyme-linked immunization. Commonly used chemiluminescence reagents include luminol, alkaline phosphatase AMPPD, and acridinium esters. Chemiluminescence has high sensitivity, high degree of automation, simple operation, stable reagents and long validity period, but the cost is relatively high. With the development of technology and the reduction of costs, the coexistence of chemiluminescence and enzyme-linked immunoassay has gradually changed.   Chemiluminescence Immunology (CLIA):   It is used for the detection of various antigens, antibodies, hormones, etc., with higher sensitivity than ELISA, strong specificity, wide linear range, stable results, and simplified operation. It is widely used in the detection of clinical specimens. This method has relatively high requirements for equipment, operation, and use environment, and it requires a matching chemiluminescence instrument when used. Chemiluminescence immunoassay and enzyme-linked immunoassay   Enzyme-linked immunoassay:   It is a highly sensitive immunological experimental technique that combines antigen, antibody-specific reaction and high-efficiency enzyme catalysis on the substrate. In the determination, after adding the enzyme reaction substrate, the substrate is catalyzed into a colored product. The chromogen substrates TOOS, ADPS, MAOS and TMB in ELISA are all commonly used color reagents. Therefore, semi-quantitative or qualitative analysis can be performed according to the shade of color. The detection method has high sensitivity and low difficulty in carrier standardization, but the detection speed is slow, easy to be contaminated, and the steps are relatively complicated.   From the perspective of the international market, the chemiluminescence immunodiagnostic method has basically tested the alternative of the enzyme-linked immunoassay method, and it accounts for nearly 90% of the market share of the immunodiagnostic market. From the statistics of the domestic immunodiagnostic market segment, after 2011, the development of chemiluminescence has accelerated, and the market share has increased from 50% to 73%, occupying an absolute dominant position. In the future, as technology advances and cost control is strengthened, the advantages of chemiluminescence will become more prominent. Taking the international market as a benchmark, there is still a lot of room for development in domestic chemiluminescence methods.   Chemiluminescence CLIA immunoassay has important guiding significance for disease diagnosis, efficacy evaluation, and screening of healthy people. Through accurate quantitative analysis of various trace active substances in human serum, it provides clinicians with an important basis for accurate diagnosis of diseases. As the gold segment of the IVD industry, its market capacity has increased from 2 billion yuan in 2010 to 6.9 billion yuan in 2015, with a compound annual growth rate of more than 25%. With the gradual continuation of chemiluminescence technology changes and substitution trends, and the continuous improvement of overall market demand, it is expected to continue to maintain a market growth of about 25%-30% in the future.   For the analysis of scientific research experiments, if it is qualitative or semi-quantitative analysis, Desheng recommends using enzyme-linked immunosorbent assay, and using TOOS, MAOS for color reagents, etc., if it is quantitative analysis of very small indicators, it is recommended to use chemiluminescence method , Luminol or acridinium ester can be used as luminescent reagent.

The prosperity during China's National Day is the most powerful signal to the world of our economic recovery. Many places abroad are still dealing with the second rebound of the epidemic, but all walks of life in China have gradually entered the right track. Like carbomer, a raw material for daily chemicals, China, which originally relied on imports, is now self-sufficient and favored by foreign manufacturers.   In terms of the overall economy, the World Bank predicts that China's GDP will grow by 1.6% this year, while the global economy as a whole will shrink by 5.2%. China has achieved a faster recovery through various measures. The effect is obvious. It is estimated that by the end of this year, China's share of global GDP may rise by about 1.1 percentage points, which is more than three times the share obtained in 2019. In contrast, the shares of the United States and Europe both declined slightly. It is estimated that by the end of the year, China’s total economic volume will be approximately US$14.6 trillion, which is approximately 17.5% of global GDP.   Regarding imported raw materials, carbomer raw materials are only one of a variety of raw materials, and there are also many daily chemicals, chemicals, food, reagents and other raw materials that are gradually showing a trend of domestic raw materials replacing imported raw materials. Although some raw materials in Europe and the United States have inherent advantages such as early industrial development and complete industrial chain, some of them are indeed ahead of domestic enterprises in terms of technology and entrepreneurship.   Carbomer raw cardboard drums ready for loading   After the outbreak of the epidemic, the restricted import of raw materials has also accelerated the research and development process of domestic high-quality raw materials, enabling many raw material production technologies including carbomer to gradually develop to the international advanced level. On the other hand, China's large population and high market demand have laid a foundation for the development of domestic reagent raw materials, and can also stimulate the potential of domestic enterprises in the development and innovation of raw materials technology.   The raw materials of carbomer, especially carbomer 980 and 940, due to its high efficiency of gelling, suspending, thickening, and dispersing in many substances without adverse effects on each other, it is used in cosmetics, shampoos, medical The position in products such as accessories is basically irreplaceable. For modern daily life, the demand for carbomer is also very large.   The performance of carbomer used in different industries is also different. In addition to being used as a gel in skin care products and cosmetics, carbomer can also be used as a hand-washing gel, which is also used more during the epidemic. A disinfectant hand sanitizer. The demand for carbomer raw materials has increased and has been recognized by many foreign companies. This also reflects the significant recovery of China's economy.