China Wuhan Desheng Biochemical Technology Co., Ltd Company News

The new coronavirus is a new strain of coronavirus discovered in the human body in 2019. There was an outbreak across the country in early 2020. At that time, experts said that the new crown virus was afraid of high temperatures, and gradually came under control as the temperature rose. Otherwise, under the scorching sun in August, the new crown did not contain its "minions" and continued to rush like people.   On August 17, at the launching ceremony of the National Air Purification Product Quality Supervision and Inspection Center, Academician Zhong Nanshan, the director of the expert committee, attended the event and delivered a speech. At the meeting, Zhong Nanshan responded to topics of public concern such as the recent new local cases and imported frozen food. Regarding the recent new local cases in Shanwei, Guangdong, Zhong Nanshan believes that Guangdong is very tight in prevention and control, and hopes to be controlled. Academician Zhong Nanshan: "Up to now, as far as I know, hundreds of thousands of people have been tested in Shanwei and Shenzhen, followed up with close contacts, and cleaned and disinfected the area. It seems that no new cases have been found. There is hope (to be) controlled the local epidemic."   In response to the public concern about the positive detection of new coronavirus nucleic acid on the imported frozen fresh food packaging, Zhong Nanshan suggested that the public should pay more attention to the contact between frozen meat and fish and wash their hands frequently. "Imported food is relatively rare, so don't overstate it."   "Wear a mask and keep your distance. After the new outbreak, we have put more emphasis on hand washing. Contaminated food, especially frozen meat and fish, should be paid special attention." Recently, sporadic cases still exist everywhere. Since most of the population is still susceptible, Academician Zhong Nanshan pointed out that the prevention and control of the epidemic cannot be let down.   "Every one of us is susceptible, and our antibody detection rate in Guangdong and Guangzhou is only a few tenths of a percent, or even a few tenths of a percent. That is to say, only zero percent. Few people have antibodies, and other people are not immune. As long as you are infected with the virus, you will get it. It turns out that we were too naive, thinking that COVID-19 is similar to SARS, and it should be better in summer (less infectious) Now it seems that summer is still like this."   The prevention and control of the epidemic is not relaxed, for the safety and health of the people across the country. Zhong Nanshan said: “We are always very vigilant about the recurrence of the epidemic anywhere. In the past six months, I have deeply felt that the philosophy of epidemic management at home and abroad is really very different. Some countries are economically first, because if The economy is not good, and some people have no food to eat. But in China, life is first. My experience of this is very profound."

Before answering this question, Desheng will share with you a basic knowledge of microorganisms: the dominant infection rate, that is, how many people will show symptoms after being exposed to pathogens. Correspondingly, there is a recessive infection, that is, after a person is exposed to the pathogen, he is fine, and he may be immunized once because of this, but at the same time, it is also possible that his own immune system cannot defeat it, causing no obvious symptoms, but the virus has always been Low-level replication, and continuous detoxification.   Now that the new crown virus is still spreading, someone asked if someone was infected with new crown pneumonia without knowing it, and then healed on their own? Through the introduction to the basic knowledge of microorganisms at the beginning, I believe you will answer correctly: this situation should exist. Today Desheng will talk to you about this issue.   The core material of nucleic acid detection-virus transport media   From the current point of view, there are two situations after the new coronavirus infects humans. One is the new coronavirus pneumonia infection, which is more common. The other is asymptomatic infection. Academician Zhong Nanshan said in an interview with CCTV News: There are not many asymptomatic infections, but there are. So, what is an asymptomatic infection?   According to the new coronavirus infection pneumonia case monitoring program, asymptomatic infections have no clinical symptoms, but respiratory specimens tested positive for the new coronavirus pathogen. If this asymptomatic infection person has strong immunity, the new coronavirus invades the body, but it is not enough to cause the symptoms of new coronavirus pneumonia such as fever, cough, fatigue, diarrhea, etc.; before long, the new coronavirus is completely affected by the autoimmune system Eliminate and heal itself. Therefore, in theory, this kind of situation may exist in theory that someone is infected without knowing it, and then heals himself.   So, is this asymptomatic infection actually contagious? The pneumonia diagnosis and treatment plan for the new coronavirus infection clearly states that asymptomatic infections may also become the source of infection. At present, many asymptomatic infections have been found. They have no clinical symptoms, but can transmit the virus to others. These asymptomatic infections may be in the incubation period, or they may only carry the virus and will not get sick at all, or they may only have very mild symptoms. They are not aware of it, or even pneumonia will not appear. With the current level of technology, it is difficult Was checked out. Asymptomatic infections who have no symptoms but are contagious are also the most difficult place to prevent and control the current epidemic. Some experts pointed out that although there are asymptomatic infections, but the number is small and the infectious power is not strong, as long as protective measures are taken, there is no need to worry too much.   In this critical stage of epidemic prevention and control, everyone should not forget when the epidemic has improved. It is important to improve immunity. Not going out, not gathering, wearing masks, washing hands frequently, and ventilating are still preventing the new coronavirus, and also responding to it. Effective measures for asymptomatic infected persons. In addition, we also need to cooperate with local policies to actively carry out nucleic acid testing.

Last week, schools in various regions notified schools of corresponding school opening time notifications. Taking Xiangyang City as an example, Xiangyang Education Bureau issued the first autumn school opening notice. The city’s general high schools, secondary vocational schools (including technical schools), junior high schools, and elementary schools are scheduled to start on September 1. ; The kindergarten is scheduled to start on September 7; each college will determine the specific time for the start of school based on actual conditions.   In order to do a good job in the investigation of health risks and to usher in the fall of school with confidence, 77,000 faculty and staff in Xiangyang city began to undergo nucleic acid testing on August 23. Hairong Elementary School is one of the schools designated for testing in the urban area. On August 23, a total of more than 700 faculty members were tested for nucleic acid. Wang Juanjuan, director of the Moral Education Department of Hairong Primary School, said that starting from the 24th, we will enter the centralized training stage and actively prepare children’s education on epidemic prevention knowledge and life safety education.   After completing the nucleic acid test, the city’s faculty and staff are required not to leave Xiangyang unless necessary. Before school starts, the school will carry out the elimination of the campus and the training of teachers on epidemic prevention. City Hairong Primary School teacher Wang Lihong said that in addition to teachers’ participation in nucleic acid testing, other people who are in close contact with students and parents are also tested. After the start of school, all classes are required to carry out the morning and afternoon inspection system and the epidemic report system. It is also understood that after the start of school, 920,000 students in the city, including kindergartens and colleges, will also implement nucleic acid testing and screening for all employees in batches.   As the school starts in September, all colleges and universities should organize staff and students to conduct nucleic acid tests to ensure a safe learning environment. A large number of nucleic acid detection processes are inseparable from the support of Virus Transport Medias. Currently, the market is generally divided into activated and inactivated types according to the composition and function of Virus Transport Medias.   The inactivated type is an inactivated virus that reduces the risk of secondary infection and has lower requirements for the detection environment. Therefore, the inactivated type is generally used for nucleic acid detection in large groups, and the risk of detection is small. The inactivated type can instantly lyse the pathogen to release the nucleic acid, and the protective agent can prevent the accounting from being degraded; and the activated Virus Transport Media can maintain the activity of the virus in a wide temperature range, keeping the originality of the sample to the greatest extent, and can be used Extraction and detection of viral nucleic acid, cultivation and separation of viruses.   The activated type contains Hanks liquid foundation, gentamicin, fungal antibiotics, BSA (V), cryoprotectant, biological buffer and amino acids. A combination of multiple antibiotics has anti-bacterial and anti-fungal effects; bovine serum albumin (BSA), as a protein stabilizer, can form a protective film on the protein shell of the virus, making it difficult to decompose and ensuring the integrity of the virus; Hanks The neutral environment constructed by the buffer helps increase the survival time of the virus and the stability of infection. The activated Virus Transport Media is usually used for the collection and transportation of clinical influenza, avian influenza (such as H7N9), hand, foot and mouth disease, measles and other virus specimens, as well as mycoplasma, ureaplasma, chlamydia and other specimens.   Desheng can provide the above-mentioned inactivated and activated Virus Transport Medias. The two preservation solutions produced have a high detection rate and can be configured for different needs of customers to provide you with high-quality products.

Chemiluminescence immunodiagnosis technology uses chemiluminescence agents, catalytic enzymes and other substances to label antigens or antibodies. When the labeled antigen or antibody is combined with the corresponding antibodies and antigens, the luminescent substrate is affected by the luminescence agents, catalytic enzymes and other substances. Redox reactions. Compared with traditional biochemical diagnosis, chemiluminescence has the advantages of high automation, good specificity, high accuracy, and wide detection range.   Chemiluminescence is a battleground for IVD giants. The luminescence products of multinational giants entered China from the beginning of the 21st century. Siemens first entered the Chinese market in 2003, followed by bioMérieux, Beckman, Johnson & Johnson, and Abbott. In 2006, Roche's electrochemistry Luminous entered China, and imported brands quickly expanded their territory. Later, multinational companies such as Soling, Sysmex, Tosoh and others have also stepped up their efforts in the Chinese market.     Since the first batch of domestically produced automatic chemiluminescence immunoassay analyzers launched by New Industries and Mike in 2011, domestic brands have joined the competition. At present, the domestic luminescence market has basically formed a "4+4" competition pattern. Four foreign giants such as Roche, Abbott, Siemens, and Beckman account for more than 70% of the market share. The top three hospitals are its main customers, and the new industry of domestic enterprises, Antu Bio , Mike Biology and Mindray Medical have nearly 10% market share.   Objectively speaking, the current domestic brands' chemiluminescence has a certain gap with imported brands in indicators such as accuracy, stability, reagent quality and instrument detection speed, and because of the coexistence of multiple technical routes of chemiluminescence detection, it is difficult for manufacturers to trace the value to the source. The product quality is proved by other methods, and the possibility of domestic brands replacing imported brands on a large scale in the short term is low. In the long run, chemiluminescence is a relatively mature detection method after all. As the quality of domestic products is gradually recognized and accepted by the top three hospitals, the low-value detection items of the laboratory will gradually be replaced. We believe that it will account for the total amount of luminescence detection. More than 30% of infectious diseases are expected to take the lead in realizing import substitution. The test results of domestic Antu Biology and other companies are almost the same as Roche's "gold standard". After five years, the overall domestic share of chemiluminescence is expected to reach more than 30%.   In terms of chemiluminescence raw materials, domestic manufacturers of chemiluminescence reagents are gradually getting rid of the current situation of relying heavily on foreign imports for core raw materials such as enzymes, antigens and antibodies. For example, Antu Bio has achieved more than 70% of raw materials in-house. With the continuous development of research and development, domestic IVD The gaps in the field of diagnostic raw materials will be gradually filled. As one of the manufacturers of R&D chemiluminescent reagents, Desheng will also occupy a place in the field of diagnostic raw materials.

Luminol is a commonly used chemiluminescence reagent. The color of its products is best white, followed by milky white, and then light yellow. Luminol developed and produced by Desheng is milky white powder, which is easily soluble in lye. It is also soluble in dilute acid, almost insoluble in water, and difficult to dissolve in alcohol. It will only show luminescence when it is used as a solution. At the same time, when we install this product, we need to use a light-shielding and well-closed container. The temperature should be guaranteed to be 0-5°C, and attention should be paid to avoid light and moisture, so as to ensure that the product inspection quality is more accurate and higher. Luminol has a wide range of uses. In addition to criminal investigation, it can also be used for immunochemiluminescence reactions, for the detection of sex hormones, drug abuse, and cardiovascular and reproductive diseases. In view of its application value and broad market prospects, the advantages of our company's patented preparation method compared with traditional preparation methods are worthy of attention. Traditional preparation method:   (1) Using 3-nitrophthalic acid as the raw material, the cyclization reaction with hydrazine hydrate is carried out, and luminol is obtained by reduction with sodium hydroxide. The synthetic method has a simple process route, but the disadvantages are: 1. The first step is high reaction temperature and requires 225°C; 2. The purification is difficult. The first step requires high boiling point triethylene glycol as a solvent, which is difficult to remove. The reducing agent used in the second step will decompose and produce several inorganic impurities during the reaction process, which are difficult to remove; 3. The yield is low, only about 30%.   (2) Using 3-nitrophthalic acid as a raw material, the cyclization reaction is carried out with hydrazine sulfate, and luminol is obtained by reduction with sodium hydroxide. The synthesis method has been improved on Route 1, but the disadvantages are: 1. The highly toxic hydrazine sulfate is used; 2. The reaction temperature in the first step is 170 degrees, which is too high and requires high equipment; 3. The reaction produces a lot of In the waste liquid, the reducing agent used in the second step will decompose and produce several inorganic impurities during the reaction, which are difficult to remove.   (3) 3-Phthalic anhydride is used as raw material, nitrated with mixed acid to obtain 3-nitrophthalic acid, dehydrated with acetic anhydride to obtain 3-nitrophthalic anhydride, then hydrazinolysis, and finally iron powder reduction Get luminol. The shortcomings of the synthesis method are: 1. The synthesis route is longer; 2. The mixed acid nitrification produces a large amount of acidic waste liquid; 3. The reduction of iron powder results in a large amount of iron slag waste, which causes great environmental pollution.   Desheng preparation method: The method of "a one-pot synthesis of luminol or isoluminol" developed by Desheng has obtained a patent. Compared with the prior art, its advantages and beneficial effects are obvious.   1) This method realizes that the three-step reaction is completed in the same pot, the intermediate product does not need any purification treatment, and the final product is directly obtained.   2) The method has simple synthesis route, mild reaction conditions, simple operation, and all the reagents required are conventional reagents, and the equipment required is conventional equipment, and the price is low. Therefore, the cost required for synthesis is low, and it is suitable for large-scale industrial production. .   3) The yield and purity of luminol and isoluminol synthesized by this method are high, the yields of luminol and isoluminol are all above 80%, and the HPLC (high performance liquid chromatography) purity is above 98% , Can fully meet the needs of industrialized production of products and market demand.

Routine blood testing is already a very important routine testing item. Platelet count is an important basis for the diagnosis and identification of hemostasis and thrombotic diseases. However, in daily testing, the platelet count using a blood cell counter may sometimes be falsely increased or Lower, why is this?   The role of platelets   Platelet is abbreviated as PLT, which belongs to blood cell like red blood cell and white blood cell. It is dimpled, oval or disk-shaped on both sides, but much smaller than red blood cell and white blood cell. The average diameter is only 2 to 4 microns. It is formed by fragments of bone marrow megakaryocyte cytoplasm shedding. . When blood vessels are damaged, platelets aggregate into clusters to form thrombus; and also release substances that promote vasoconstriction and blood clotting. Platelet count refers to the number of platelets contained in a unit volume of blood. Certain diseases can lead to a decrease or increase in the number of platelets.     Causes of false elevation of platelets   1. In some hemolytic diseases, hemolysis occurs in the blood vessels, and then the fragments generated by the rupture of red blood cells will be divided into the platelet area by the hemocytometer, which will cause errors in the instrument and falsely increase the count. For example, hemoglobinopathy and G-6-PD deficiency can cause intravascular hemolysis.   2. After the treatment of chronic granulocyte patients, a large number of fragments will appear in the blood vessels. These fragments will also be divided into the platelet area by the hemocytometer, which will cause interference and falsely increase the platelet count.   3. Take venous blood for routine blood testing within a short period of time after transfusion of fat emulsion, chylo particles will also be counted into platelets by the blood cell counter, resulting in high falsehood. All the above three types can be corrected by observing the morphology of platelets and red and white blood cells through artificial blood smears.   Causes of false reduction in platelets   1. EDTA-K2 anticoagulant causes platelet aggregation, which is related to the presence of cryptic antigens on the surface of platelets, causing the blood cell counter to treat the adhered platelets as non-platelets, resulting in a false decrease in counts. At this time, citrate can be used instead of EDTA-K2 as an anticoagulant, or platelets can be manually counted to correct.   2. Cold agglutinin can agglutinate red blood cells, nucleated cells, and platelets, thereby making the platelet count falsely lower. When encountering the interference of cold agglutinin in the body, the specimen can be incubated at 37 degrees for a period of time to eliminate the interference caused by cold agglutinin.   3. The invisible aggregation of platelets in hypercoagulable blood will make the count falsely lower. For example, hypermagnesium, hypercholesterolemia, and high triglycerides will all increase platelet aggregation.   The instrument used for blood cell analysis has the advantages of speed and simplicity, but it is also easy to cause errors. As an established blood test reagent company, Desheng recommends that when the platelet count does not match the clinical practice, it must be corrected by manual recheck to avoid unnecessary disputes between doctors and patients.

As the weather becomes hot, all chemical companies are in great danger. It is necessary to strictly pay attention to the storage conditions of raw materials and the temperature control during equipment operation to avoid safety accidents. According to the recent notice issued by the Jiangsu Provincial Emergency Management Department, chemical (hazardous chemicals) companies are required to implement eight major risk management and control measures, and do a good job in safe production of hazardous chemicals in summer and flood seasons, and make every effort to prevent and contain production safety accidents.     The eight control measures are as follows:   One is to earnestly implement monitoring measures for major hazard sources, strengthen the temperature and pressure monitoring of hazardous chemical production equipment and storage facilities, prohibit over-temperature, over-pressure, and overload production, pay close attention to the working conditions of heat exchange equipment and cooling water, and handle temperature in a timely manner , Liquid level, pressure and other abnormal conditions.   The second is to strengthen the maintenance of equipment and facilities. All automatic control and safety instrument systems, flammable and toxic gas detection and alarm systems, etc. should be put into normal operation, and buildings (structures), production equipment, storage facilities, electrical equipment, safety instruments, etc. Lightning protection and grounding conditions completely eliminate hidden dangers and effectively prevent production safety accidents caused by lightning.   The third is to improve ventilation and cooling facilities. In places involving the production, storage, and use of flammable, explosive, toxic, harmful and other hazardous chemicals, it is necessary to ensure that the ventilation, cooling, detection and alarm facilities are intact and in a normal applicable state.   The fourth is to reasonably adjust the work and rest time, arrange the time reasonably according to the weather forecast and weather changes, reduce outdoor outdoor work activities at noon high temperature, and equip heatstroke prevention and cooling drugs to prevent personnel from heatstroke. Enterprises should strictly implement the safety management regulations for the loading and unloading of hazardous chemicals in summer. When the temperature exceeds 35°C, all hazardous chemical loading and unloading operations shall be stopped from 10 am to 15 pm.   The fifth is to strengthen the safety management of inspection and maintenance, fully identify the risks of special operations such as hot work, operations in restricted spaces, temporary electricity use, and climbing operations, conduct inspection and analysis, standardize the approval of work permits, and effectively confirm the implementation of safety measures.   The sixth is to do a good job in flood prevention. Enterprises should inspect the drainage facilities in the plant area, dredge drainage, flood drainage ditches, arrange flood control materials, and implement flood control rescue teams. It is necessary to clarify the responsible personnel and regularly monitor the flammable and toxic gases in the sewage pipe network to prevent leaked materials from entering the sewage pipe network and ensure the safe operation of the pipe network. Enterprises along the river, lake, and river should pay close attention to flood forecasts, inspect flood discharge isolation gate facilities such as the river, lake, and river, arrange flood control personnel on duty, and be ready to close the gate at any time to prevent rivers, lakes, and rivers from flowing. Backflow.   The seventh is to inspect and test the fire sprinkler system, and conduct a start-up test on the sprinkler cooling water system installed in the hazardous chemical tank area to ensure that the water pressure and cooling effect meet the design requirements.   The eighth is to pay attention to the safety of enterprises that stop production, and urge enterprises to fully understand the situation of hazardous chemicals in storage tanks and installations, and properly handle, recover and transfer hazardous materials. Strengthen the monitoring and control of out-of-service production installations and storage facilities that contain hazardous chemicals, and shall not dismantle or disable safety facilities without authorization.   Desheng specializes in the research and development, production and sales of blood collection tube additives, in vitro diagnostic reagents, buffers, and luminescent substrates. The storage of raw materials, equipment monitoring, and mental state supervision of production personnel are all strictly supervised to avoid safety accidents. In the scorching summer, Desheng hopes that all chemical companies will attach great importance to safe production.

Chemiluminescence technology is widely used in clinical practice. Chemiluminescence is a type of immunodiagnosis, which uses the specific reaction between antigen and antibody to determine the concentration of disease markers in the body to determine the body state of the human body. It is widely used in infectious diseases, heart diseases, tumors, pregnancy detection, etc. Compared with other immunodiagnostic methods, chemiluminescence has become the mainstream of immunodiagnosis due to its systematic advantages in safety, automated operation, test accuracy, and test speed. It surpasses other technologies in performance.  chemiluminescent-reagent While chemiluminescence products have been widely used in clinical practice, they also encountered some difficult development problems, mainly in the following aspects. The biggest reason is the low concentration of markers and the difficulty of detection. 1. The fundamental reason is that the concentration of the test substance (antigen, antibody) in the sample is low, which requires more precision and standardization from R&D, production to use-the content of carbohydrates, fats, etc. in biochemical diagnostic tests are common in the human body It is the g/L-μg/L level, while the immunodiagnostic target antigens and antibodies are only at the μg/L-pg/L level, and the difference between the two is about 106 times. 2. The instrument and reagents are closed, and the development capabilities of the enterprise's instruments and reagents are very high. The instrument determines the lower limit, and the reagent determines the upper limit; 3. Cutting-edge technologies in various fields are required: mechanical automation, optics, materials, statistics, etc. There are obviously insufficient talents in the field, and it is difficult to train; 4. High R&D cost and long cycle. Compared with the differences in different types of technologies, domestic manufacturers are more important to product development and quality control capabilities. As one of the leading manufacturers of chemiluminescence reagents, Desheng should have higher requirements for the quality of products. Desheng’s chemiluminescence reagent products mainly include acridinium esters, luminol and isoluminol, and rely on super high Cost-effective, professional scientific research and technical team, and strong after-sales service have been unanimously recognized by many customers.

People basically use in vitro diagnostic reagents (IVD) when they go to the hospital or go for a physical examination. In vitro diagnostic reagents are the "doctor's eyes", which can assist clinicians in diagnosing the condition, observing curative effects, and adjusting treatment plans. Product quality and safety level are an important basis for ensuring the correctness of relevant test results and diagnosis.   As people’s demand for health and disease prevention and treatment services grows, in the process of medical and health services, the public’s awareness of the quality and safety of diagnostic reagents is getting stronger and stronger. For the test results of one item in multiple hospitals, for one item The comparison of test results in different time periods in the same hospital has begun to require knowledge and attention to quality control, which also requires high attention and common progress from all aspects of society! Different colors of in vitro diagnostic reagents At present, most of the in vitro diagnostic reagents in my country are regulated in accordance with medical device standards, and some are regulated in accordance with drug standards. The popular science knowledge introduced here is mainly aimed at how to buy correctly, understand the technical parameters that need to be paid attention to when purchasing products, transportation requirements, and storage requirements during use. It has certain guidance and help for readers to understand, correctly understand and apply in vitro diagnostic reagents. Here are a few frequently asked questions and answers.   1. How to judge the technical performance of in vitro diagnostic reagents? Answer: The performance of in vitro diagnostic reagents is mainly reflected in three aspects: 1. Analytical performance: mainly includes precision, accuracy, sensitivity, specificity, linear range or measurement range, etc., which may be reflected in some technical indicators in the product manual There is not a complete agreement. 2. Diagnostic performance: the degree of sensitivity and specificity to the tested substance. 3. Stability: the production date, expiration date, expiration date, and calibration requirements of the product.   The raw materials and processes used in the reagents should have clear quality requirements and have been verified. The performance of the final product meets the requirements for clinical use. The main factors affecting product performance include the establishment of raw materials, processes and reaction systems, performance evaluation methods and methods, the establishment of internal reference products, and clinical evaluation.   2. What are the special requirements for the storage of in vitro diagnostic reagents? Answer: The storage of in vitro diagnostic reagents is quite special and requires certain conditions. According to the types and performance of in vitro diagnostic reagents, product partitioning and classified storage management should be implemented. The product storage warehouse shall meet the requirements of temperature and humidity, dustproof, ventilation, protection from light, and storage period regulations, and shall be equipped with temperature and humidity monitoring and control facilities or equipment, and maintain monitoring records. Most in vitro diagnostic reagents need to be stored in a low temperature between 2-8℃, a few varieties need to be frozen, and some varieties can be stored at room temperature. The specific requirements are clearly marked in the product manual.   3. Are there special requirements for the transportation of in vitro diagnostic reagents? Answer: The transportation of IVD reagents should meet the requirements of transportation conditions and product packaging. Product manuals and labels that require low temperature and refrigeration shall be transported and stored in low temperature and refrigeration facilities in accordance with relevant regulations.

The EDTA anticoagulant blood routine test is the most basic test for blood testing, but whether it is better to use venous blood or peripheral blood for the test, there are different opinions in the industry. Blood routine involves the detection of multiple indicators, and it is necessary to compare the data of each indicator measured by two blood sampling methods.   The popularity and application of blood analyzers have greatly improved the level and efficiency of blood testing, laboratory science continues to develop, and the test results are more accurate. After comparing the data reproducibility and corresponding data of the two blood samples, it is found that the data of venous blood collection has good reproducibility, while the reproducibility of peripheral blood collection is relatively poor, and the data obtained by the two methods are not the same. https://www.vacutaineradditives.com/ Repeatability test results: There is no significant difference in the repeatability of the white blood cell (WBC), red blood cell (RBC), and hemoglobin (HGB) test results of venous blood sampling (P>0.05), while the difference in the repeatability of peripheral blood sampling is significant (P

In terms of detection, the higher the sensitivity, the better. The higher the purity of the reagent, the better the detection effect will be? The more expensive the reagent, the better the effect? Actually not. For diagnostic reagents, choosing the right reagent is the most important. In vitro diagnostic reagents   Does the higher the sensitivity of the reagent mean the best effect? Not entirely, usually the higher the sensitivity means that the analyte with a very small content can also be detected, but the detection index content in the sample to be tested is high, and there is no need to require high sensitivity of the reagent; if the composition of the sample to be tested is complex , Reagents with high sensitivity may also detect some interfering substances, resulting in inaccurate final data. Therefore, in biochemical detection, in order to eliminate interference, MAOS will be used as the chromogen substrate; for the low content analyte, TOOS with high sensitivity is used as the chromogen.   Is the purity of the reagent the better? No. Generally speaking, when purchasing diagnostic reagents, you usually pay attention to the purity data on the test report. The higher the purity, the better the quality. But for a specific experiment, the higher the purity, the better. Such as the simplest reagents, deionized water and ultrapure water. Some biochemical experiments can use deionized water. If ultrapure water is used, it means that all the reagents involved use ultrapure water, which increases the cost and the experiment operation becomes cumbersome.   As long as the reagent purity and impurity ion content can meet the needs of the experiment, the experiment can be carried out normally. It is not necessary to use high-purity reagent grade Tris for industrial synthesis of Tris, and injection grade heparin is not necessary for heparin used for anticoagulation. The purification cost of some reagents is relatively high, and the performance of some reagents can be improved by adding a small amount of other substances.   In vitro diagnostic reagents are used for scientific experiments in biochemical testing. It is a very rigorous work. The applied reagents need to be paid attention to, otherwise the test results will lose the significance of testing if they are inaccurate. Therefore, in the selection of reagents, it is necessary to choose the most suitable one to achieve the best results.

In a recent murder case in Hangzhou, the police used lumino technology to repeatedly wash blood stains to reveal the original shape, even if it was washed with strong acid, it would leave corresponding traces. DNA technology finds tissue DNA particles and blood traces, and other technologies find body tissues with victims’ DNA in pipes and septic tanks. It can be seen that no bloodstain can escape Rumilo's detection.     The luminol reaction is a classic chemiluminescence reaction. It is a yellow crystal or beige powder at room temperature, which is a relatively stable chemical reagent. In forensic medicine, the luminol reaction is also called the aminophthaloyl-hydrazine reaction. It is a chemical reaction that cannot be observed with the naked eye when detected at the crime scene. It can show a very small amount of blood stains (occult blood reaction), even after scrubbing. , Or bloodstains long ago can also be detected by luminol. Biologically, luminol is used to detect the presence of copper, iron and cyanide in cells.   The initial application of luminol reagent was to detect proteins. In western blotting, an enzyme-linked antibody is often used to bind to the protein to be detected, and the combination of luminol chemiluminescence reagent and enzyme (peroxidase) can make local luminescence and reduce the protein The position is displayed.   In addition, luminol derivatives such as isoluminol (ABEI) are labeled on carboxylic acid and ammonia compounds, and then separated by high performance liquid chromatography (HPLC) or liquid chromatography (LC), and then under alkaline conditions It reacts with hydrogen peroxide-potassium ferricyanide to perform chemiluminescence detection, such as labeling the newly synthesized chemiluminescence reagent isoluminol isothiocyanate to yeast RNA, separating it by centrifugation and dialysis, and then performing chemiluminescence detection.   But we should still pay attention to some issues that need to be paid attention to when using luminol and several things that need to be paid attention to when investigating:   1. Luminol fluoresces in the presence of iron, iron-containing alloys, horseradish or certain bleaching agents. Therefore, if the crime scene is thoroughly treated with bleach, the fluorescence from luminol will strongly mask any blood stains.   2. Luminol may interfere with other tests, but luminol does not interfere with DNA extraction.   3. The use of luminol needs to be in a dark environment, so that it is easier to make more accurate judgments with the naked eye.   4. Luminol has a limited amount of time to glow, so you must hurry up to take photos and record.   5. Luminescence lasts for about 30 seconds, which can be observed through long-exposure photos, and the surrounding environment should not be too bright.   In addition to luminol, the chemiluminescence reagents developed by Desheng also include acridine esters and other series. The effect is stable and the quality is guaranteed.