Acridine ester DMAE·NHS is a typical acridine chemiluminescence reagent that has been commercialized. It has the characteristics of high sensitivity of chemiluminescence analysis, simple equipment, no light source to avoid the influence of astigmatism, and high reproducibility. Important fields such as environmental monitoring, clinical analysis, and biochemistry have been widely used.
Acridine chemiluminescence reagents for detection
Development of acridine chemiluminescence reagents:
Acridine Since Grebe and Carlo discovered acridine in the high-boiling fraction of coal tar in 1870, acridine and acridine derivatives have attracted a lot of scientific and technological interest, and have subsequently been widely used in the development of anti-cancer drugs and antibacterial agents. , Immunodiagnosis and other fields. Acridine chemiluminescent reagents are a technologically advanced luminescent marker for proteins, antibodies, and nucleic acids in immunodiagnosis.
Advantages of acridine ester DMAE-NHS:
Compared with other luminescent substrates such as luminol and AMPPD, acridine-based luminescent reagents have high luminescence intensity, high efficiency, low background interference, no need for enzyme catalysis and enhancers, high signal-to-noise ratio and label binding Stability and other advantages. Moreover, after structural modification of acridine compounds, a chemiluminescence probe is made, which is not only suitable for labeling small molecules, but also for labeling biomacromolecules with chemiluminescence, which can label proteins, proteins under neutral or slightly alkaline conditions. Biological macromolecules such as nucleic acid and polypeptide, and the fluorescence quantum yield and biological activity of the labeled substance will not be damaged.
Comparison of acridine ester DMAE-NHS with other acridine luminescent reagents:
There are more than ten kinds of acridine chemiluminescence reagents commercialized on the market, of which 6 are developed and produced by Desheng, including 3 acridine esters and 3 acridine sulfonamides. Compared with other luminescent reagents, the acridinium ester DMAE-NHS is characterized by containing NHS groups, which can be directly connected to the primary amino groups of proteins or antibodies for labeling. There are also some differences in hydrophilicity from acridine sulfonamides. Choose the appropriate acridinium ester for the type of protein or antibody.
It should be noted that Desheng’s acridinium ester DMAE-NHS has improved hydrophilicity on the basis of traditional acridinium ester, but its dissolution process still needs to be strictly anhydrous. In addition, mercapto groups may cause acridine ester hydrolysis. Mercaptoethanol cannot be used, and care should be taken if the labeled protein contains sulfhydryl groups.
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