China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Specific uses of cosmetics   Desheng produces high-quality Good's Buffer, and can customize product packaging and some indicators according to customer needs. Many buffers in Good's Buffe are used in cosmetics, such as toners, essences, lotions, eye creams and facial masks. Here are three kinds of Good's Buffer commonly used in cosmetics, as follows:   1) HEPES buffer CAS Number: 7365-45-9 Useful pH range: 6.8-8.2 Uses and precautions: ◆Acidity regulator or pH control agent. ◆Identified that the effective concentration of HEPES can provide exfoliation. ◆Promote the transdermal absorption of various functional ingredients in cosmetics. 2) TRIS CAS number: 77-86-1 Useful pH range: 7.2-9.0 Uses and precautions: ◆Non-ionic surfactant-amine. ◆Acidity regulator or pH control agent. ◆Excellent cleaning ability and emulsifying ability. 3) BIS-TRIS CAS Number: 5625-37-6 Useful pH range: 6.1-7.5 Uses and precautions: ◆Non-ionic surfactant-amine. ◆Acidity regulator or pH control agent. ◆Excellent cleaning, dispersing, emulsifying and antistatic ability. In fact, there are many buffers that are suitable for use in cosmetics and play different roles, but the prerequisite is good quality, because after all, the use of cosmetics is still on the human body. Most buffers are used in cosmetics to adjust the pH of their raw materials or solvents. Of course, there are also surfactants such as the above-mentioned surfactants, and some of them have exfoliating effects to promote skin absorption. In terms of dosage, different cosmetics increase the amount differently.

We generally know that luminol is a chemiluminescent reagent, which is generally used for blood testing. It is a yellow crystal or beige powder at room temperature. It is a strong acid and has a certain irritating effect on eyes, skin and respiratory tract. Under alkaline conditions, it can be oxidized by peroxides and emit light at the same time. The redox reaction between luminol and peroxide requires a catalyst. This catalyst is generally a multivalent metal ion, peroxidase such as iron and horseradish. Peroxidase, etc., this method is often used to detect the content of peroxides, heavy metals, peroxidase, and the derived free radicals, for toxicant analysis and analysis methods based on peroxidase and glucose oxidase .                                                                               Luminol Luminous Picture It is our incomplete understanding of it that leads to many questions about him in many cases. 1. When luminol detects blood, can it distinguish between human and animal blood? Answer: No, because luminol detects blood mainly using the catalysis of iron in hemoglobin, and animal hemoglobin is not much different from humans, so luminol can only detect blood and cannot distinguish what kind of blood it is. If you accidentally mix human blood with blood from other animals, it is impossible to determine whether it is human blood just with luminol. But luminol has a good advantage that it does not affect DNA changes, so as long as we can detect blood, we can distinguish between human blood and other blood in DNA comparison.   2. Will the blood be washed with detergent affect the detection of luminol? Answer: If the criminal suspect takes some measures after committing the crime, such as cleaning the scene with hypochlorous acid bleach, as long as he sprays luminol on the crime scene that has been washed with hypochlorous acid, it will glow everywhere, which will interfere with blood stains. Detection. However, although both luminol and hypochlorous acid can make luminol emit light, the two luminescence phenomena are different. The luminescence caused by bleach flashes quickly, while the luminescence caused by blood stains appears gradually.   3. Can we continue to use luminol after one reaction? Answer: The luminescence principle of luminol is because hemoglobin contains iron, and iron can catalyze the decomposition of hydrogen peroxide, which turns hydrogen peroxide into water and monooxygen, and monooxygen oxidizes luminol to make it glow. When we use luminol, it will not change and oxidize the iron ions in the blood, and iron ions can continue to play a catalytic role as a catalyst. Therefore, the light-emitting process can continue to react. So the second use of luminol can still see the light.   4. Is it toxic if it accidentally sticks to the hands when using luminol? Answer: The skin absorbs luminol very poorly. Even if luminol enters the body through the skin or other means, it will not deposit in the body or cause chronic toxicity. Moreover, there has been no report of luminol poisoning caused by improper protection. It can be inferred from the above that even if luminol easily enters the blood through the mucosa of the gastrointestinal tract, it will be quickly excreted from the urine. The chance of luminol passing through the skin or depositing in the body is very small, thus causing chronic poisoning. The possibility is very small.   The above are a few common questions we have sorted out through customer feedback, and hope that we can really help you in the follow-up use. Desheng Technology has more than ten years of experience in the field of chemiluminescence reagents. In addition to luminol, we also have acridine esters and their derivatives, which have left a good reputation for customers. We will continue to do our products with heart.

A pandemic caused carbomer...Carbomer raw materials are mainly used as daily chemical products, skin care creams, lotions, etc., as well as the gel matrix in various medicinal excipients. It has efficient thickening, gelation, and suspension. Performance etc. Because of its good water absorption and suspending properties, it has become one of the main raw materials in the disposable hand sanitizing gel that is widely used during the epidemic. This hand sanitizer is not liquid and will not flow randomly, and it has disinfection, Features such as no-washing are very suitable for quick disinfection of hands.                                                                        Desheng Carbomer 940 powder    Due to its indispensable position in the daily chemical, pharmaceutical and other industries, high-quality carbomer raw materials have always been urgently demanded products in the market. The special circumstances affected by the epidemic this year have made Carbomer a popular raw material this year. There are countless companies that provide carbomer raw materials. It can be said that buyers are confused. What kind of carbomer raw materials company is more worth choosing?   1. Product quality is reliable   Desheng conducted a small trial production of carbomer in October 2019. At the beginning of 2020, the company invested a lot of capital, manpower and material resources. After the R&D staff worked overtime, the final test of carbomer was completed after several tests. The carbomer raw materials produced are up to standard in all indexes, which are comparable to international brands. Its transparency is better than that of well-known brands.   2. Good sales service   Desheng provides one-stop procurement services. After purchasing carbomer raw materials, customers may still encounter problems such as dissolution and agglomeration, how to use carbomer powder to make gels, etc., which require special guidance, which requires the supply of selected raw materials The supplier can provide the corresponding after-sales service. From this point of view, it is more appropriate for customers to choose some carbomer raw materials companies with good market reputation and more professional sales services.   Third, the product is cost-effective   When choosing what kind of carbomer raw material company to cooperate with, the quotation provided by the relevant company is also an important reference standard, especially when the demand for carbomer is large, the corresponding raw material purchase volume will also double. If the supplier can provide a more competitive quotation, there will definitely be more room for maneuverability in terms of cost control. Desheng is the manufacturer of Carbomer, direct sales of raw materials. So compared with other traders, the product cost performance will be higher.   I believe that after reading the above analysis, Desheng will be your worthy carbomer supplier. Now the company has begun to implement mass production, with an average daily output of 1t, and has rich experience in exporting. If necessary, please call for detailed inquiry.

National Day has passed, and 2020 is entering the final quarter, but the global epidemic has not planned to end over time. Although the country has achieved victory in the fight against the epidemic, in order to prevent a second outbreak, all walks of life cannot be taken lightly. In this situation, what should I pay attention to when purchasing reagents that are already cumbersome? The procurement of biochemical reagents, especially in vitro diagnostic reagents, is different from many bulk commodities. The method of “being afraid of comparing goods without knowing the goods” is usually not applicable. If it is a large quantity of chemical raw materials, the purchase period is usually large and the amount is relatively easy to handle. If it is a small amount of reagents that are purchased frequently, and there are many types, it will be a headache for purchasers. But there are still some ways to improve this situation. Procurement of in vitro diagnostic reagents 1. Learn more about professional knowledge As the saying goes, a worker must first sharpen his tools if he wants to do his job well, and mastering a certain degree of professional knowledge can often have a multiplier effect for purchasing. Like in vitro diagnostic reagents, there are many things that cannot be judged by name, and sometimes even the same CAS number may not be the product to be purchased. Different reagents have different grades, concentrations, purity, etc. in different applications. 2. Make good use of network information In fact, before the epidemic, current purchasers would use the Internet to purchase various reagent products. After industry exhibitions have decreased and travel is not convenient, it has become more common to purchase via the Internet. However, online procurement also has its drawbacks. A lot of information is not authentic enough, and it is inevitable that you will be deceived. You must be careful about this. Moreover, due to the situation of the epidemic, illegal incidents of dishonesty on the Internet have occurred from time to time. 3. Make records and preparations Due to the unpopularity of in-vitro diagnostic reagents and the variety of names, some products are very troublesome to find on the Internet, which often delays the procurement time to the nearby delivery time. At this time, purchasers are often more anxious, and it is easier at this time They are fooled into buying fake products, or products of poor quality, and even directly defrauded of money. Therefore, it is necessary to record and prepare related reagent products at ordinary times to avoid panic when looking for goods temporarily. Due to the impact of the epidemic, in order to seek new opportunities, many companies may also purchase some diagnostic reagents that were not familiar in the past. Therefore, it is necessary to communicate and learn more with technicians when purchasing. At the same time, you can also consult professionals like Desheng for the corresponding products. Reagent manufacturing enterprises.

The full name of Tris base in Chinese is Tris, CAS77-86-1, which is an important biological buffer and a reference material for acid titration, and has an extremely wide range of social applications. At present, there is a lot of information on the application of Tris in the fields of biochemistry and medicine, but in fact, Tris is also widely used in various researches. This article will summarize the uses of Tris.   Biological buffer:   The most well-known application of Tris is as a solvent for nucleic acids and proteins and as a biological buffer. Tris-HCl, NaCl, tween20 these three substances can form a TBST buffer, which is a commonly used washing buffer in Western Blotting; Tris-HCl can be made into TE buffer by adding EDTA to it, which can be used for DNA stability and Storage; Replace the acid solution that adjusts the pH value with acetic acid to obtain TAE buffer, and change to boric acid to obtain TBE buffer. These two buffers are often used in nucleic acid electrophoresis experiments.   Tris itself is alkaline. Only the Tris-HCl buffer system can be used to prepare buffers with a pH range from acidic to alkaline. The method is relatively simple, and it can also be combined with other substances to form a variety of buffer systems; it can interfere with biochemical processes. It is small and does not precipitate with calcium, magnesium ions and heavy metal ions. However, the pH value of Tris buffer is greatly affected by the concentration and temperature of the solution. When diluted ten times, the pH value of the buffer changes more than 0.1. For every degree of temperature rise, the pH value drops by 0.03; at the same time, it easily absorbs CO2 in the air. Therefore, the prepared buffer should be tightly sealed.   Surfactant refers to a substance that can significantly change the interface state of the solution system by adding a small amount, and has a fixed hydrophilic and lipophilic group, which can be aligned on the surface of the solution. The application of surfactants can be divided into civil and industrial applications. Two-thirds of civil surfactants are used in personal protection products, and industrial surfactants are the sum of surfactants used in various industrial fields other than civil surfactants.   The molecular structure of surfactants is amphiphilic: one end is a hydrophilic group, the other end is a hydrophobic group; the hydrophilic group is often a polar group, such as carboxylic acid, sulfonic acid, sulfuric acid, amino or amine group and Its salt, hydroxyl group, amide group, ether bond, etc. can also be used as polar hydrophilic groups; while hydrophobic groups are often non-polar hydrocarbon chains, such as hydrocarbon chains with more than 8 carbon atoms. Tris contains three hydroxyl groups and one amino group, both of which are good hydrophilic groups. Therefore, Tris is also an important intermediate for the synthesis of surfactants.   Vulcanization accelerator is a substance that can promote vulcanization, which can shorten the vulcanization time, lower the vulcanization temperature, reduce the amount of vulcanizing agent and improve the physical and mechanical properties of rubber. The accelerator with Tris as the intermediate is mainly used as the hardener of resin and coating; also used in the ink of printed circuit board, electrical insulating material, resin mold, screen printing coating, mold, adhesive, lining Materials and plastic stabilizers, etc.   In the medical field, the application of Tris is mainly divided into pharmaceutical intermediates and medicine. Tris is an intermediate of fosfomycin tromethamine. Fosfomycin tromethamine is a broad-spectrum antibacterial drug marketed in the late 1980s. Its bioavailability is comparable to that of the commonly used antimicrobial fosfomycin calcium. 3~6 times, more suitable for the treatment of urinary tract infections and other diseases.   Tris is also a drug used to treat blood diseases, and can be used for acute metabolic acidosis and acute respiratory acidosis. As a sodium-free amino buffer base, it combines with H2CO3 in body fluids to generate bicarbonate. Therefore, it can buffer acid and reduce CO2 tension, and has the dual effect of correcting acute respiratory acidosis and metabolic acidosis. Its effect is faster and stronger than sodium bicarbonate. It is especially suitable for patients with heart failure and acidosis. It is also suitable for the rescue of hypothermic anesthesia, shock and cardiac arrest. After organ transplantation, this drug can be used to treat ischemic intracellular acidosis. .   Tris has three equal hydroxyl groups and can be used as a good reducing agent. Some researchers use Tris as a reducing agent to reduce the chloroauric acid solution by ultrasound under alkaline conditions without adding other stabilizers to prepare relatively stable gold nanoparticles, realizing environmentally friendly and biocompatible gold nanoparticles Synthesis of gold nanoparticles. The experimental conditions are mild, the operation is simple and easy, and it is green and pollution-free, low-toxic and low-cost.   Tris is widely used, from laboratory biochemical experiments, to industrialized mass production, to daily necessities, it is indispensable. Speaking of its toxicity, it is a piece of cake compared to some daunting reagents, but the deep erosion has to be prevented. Tris can be harmful to the body through inhalation, ingestion, and skin absorption. Therefore, for field experimenters, wearing gloves and goggles during operation is an effective protective measure, and safe production can be used with confidence.   Desheng is a professional manufacturer of biological buffer raw materials, and Tris is the company's main advantage product. Desheng’s main product biological buffer is a kind of alcohol and ammonia fine chemicals with special characteristics. It does not participate in and does not interfere with the biochemical process. It can be used as a buffer system for life science research to provide a stable pH environment for experiments. Desheng biological buffer Agents are widely used in in vitro diagnostics, biopharmaceuticals, biological cosmetics, coatings and paints and other industries. Due to the high buffering efficiency and quality of the products, there are now dozens of stable customers. Desheng provides one-stop procurement services. If you need it, please click on the official website to consult customer service.

Currently, heparin sodium is mainly derived from the intestinal mucosa of pigs, cattle or sheep. However, heparin steel of bovine and sheep origin is at risk of being infected by related viruses, and the incidence of adverse reactions such as thrombocytopenia and thrombosis syndrome is much greater than that of pig-derived heparin steel. Therefore, in clinical use, people should try to choose porcine heparin sodium. However, in actual production, due to various reasons such as complex sources of production materials, the production raw materials from pigs are often contaminated by animal materials from cattle and sheep. Therefore, the establishment of methods for the identification of ingredients from pigs, sheep, cattle and other animal sources is essential to control the quality of drugs and prevent the contamination of xenogeneic animal-derived ingredients.   The following briefly talk about the detection principle of different species of heparin sodium, the detection principle: heparin sodium is a glycosaminoglycan, which is composed of uronic acid (L. iduronic acid, IdoA; D-glucose Uronic acid (GlcA) and glucosamine (6[.D-glucosamine, GlcN) are a mixture of polysaccharide chains with different chain lengths. Heparinase is used to enzymatically hydrolyze heparin sodium from different species, and its enzymatic hydrolysis products are analyzed and studied; that is, the species source of this product is characterized by the composition ratio of the enzymatic hydrolysis products (disaccharide mixture).   Heparinase is a type of polysaccharide lyase that acts on heparin or heparan sulfate molecules; there are three types of heparinase, namely heparinase I, heparinase II and heparinase III. Since each heparinase has a unique substrate specificity, they cut a specific site in the heparin chain. Heparinase 1 decomposes the Oc(1-4) glycosidic bond between GlcNs(6S) and IdoA(2S), and can cut off the antithrombin III binding pentasaccharide site in the heparin molecule; heparinase II can decompose two The sugar contains 2 or 3 sulfated groups of heparin, so its specificity is not strong; heparinase II1 decomposes the 6-position unsulfated GlcNS or IV. Heparin of Acetyl Glucosamine (GlcNAc) (see Figure 1). The proper mixing of the three heparinases can completely degrade heparin into an unsaturated disaccharide mixture with ultraviolet absorption at 234nm.   The detection of the source of heparin species mainly uses quantitative PCR to detect heparin sodium from different species, but this method is costly. The heparinase enzymatic hydrolysis method can effectively identify heparin samples mixed with sheep-derived crude heparin, and is simple and fast. The only disadvantage is that heparinase will lose the information of the uronic acid epimer during the enzymatic hydrolysis of heparin. Cannot distinguish between glucuronic acid and iduronic acid. In addition, in recent years, there have been methods for separating various disaccharides by capillary electrophoresis. Reversed-phase ion-pair chromatography can also be used to separate heparin disaccharides, and combined with mass spectrometry for quantitative analysis.   Desheng is a professional manufacturer of blood collection reagents and also has a complete detection system. The heparin sodium produced by it is mainly used as an additive for blood collection tubes. Heparin sodium has good anticoagulant effect, potency, and fast delivery.    

Chemiluminescence immunoassay is an in vitro diagnostic reagents method developed after enzyme-linked immunization. Commonly used chemiluminescence reagents include luminol, alkaline phosphatase AMPPD, and acridinium esters. Chemiluminescence has high sensitivity, high degree of automation, simple operation, stable reagents and long validity period, but the cost is relatively high. With the development of technology and the reduction of costs, the coexistence of chemiluminescence and enzyme-linked immunoassay has gradually changed.   Chemiluminescence Immunology (CLIA):   It is used for the detection of various antigens, antibodies, hormones, etc., with higher sensitivity than ELISA, strong specificity, wide linear range, stable results, and simplified operation. It is widely used in the detection of clinical specimens. This method has relatively high requirements for equipment, operation, and use environment, and it requires a matching chemiluminescence instrument when used. Chemiluminescence immunoassay and enzyme-linked immunoassay   Enzyme-linked immunoassay:   It is a highly sensitive immunological experimental technique that combines antigen, antibody-specific reaction and high-efficiency enzyme catalysis on the substrate. In the determination, after adding the enzyme reaction substrate, the substrate is catalyzed into a colored product. The chromogen substrates TOOS, ADPS, MAOS and TMB in ELISA are all commonly used color reagents. Therefore, semi-quantitative or qualitative analysis can be performed according to the shade of color. The detection method has high sensitivity and low difficulty in carrier standardization, but the detection speed is slow, easy to be contaminated, and the steps are relatively complicated.   From the perspective of the international market, the chemiluminescence immunodiagnostic method has basically tested the alternative of the enzyme-linked immunoassay method, and it accounts for nearly 90% of the market share of the immunodiagnostic market. From the statistics of the domestic immunodiagnostic market segment, after 2011, the development of chemiluminescence has accelerated, and the market share has increased from 50% to 73%, occupying an absolute dominant position. In the future, as technology advances and cost control is strengthened, the advantages of chemiluminescence will become more prominent. Taking the international market as a benchmark, there is still a lot of room for development in domestic chemiluminescence methods.   Chemiluminescence CLIA immunoassay has important guiding significance for disease diagnosis, efficacy evaluation, and screening of healthy people. Through accurate quantitative analysis of various trace active substances in human serum, it provides clinicians with an important basis for accurate diagnosis of diseases. As the gold segment of the IVD industry, its market capacity has increased from 2 billion yuan in 2010 to 6.9 billion yuan in 2015, with a compound annual growth rate of more than 25%. With the gradual continuation of chemiluminescence technology changes and substitution trends, and the continuous improvement of overall market demand, it is expected to continue to maintain a market growth of about 25%-30% in the future.   For the analysis of scientific research experiments, if it is qualitative or semi-quantitative analysis, Desheng recommends using enzyme-linked immunosorbent assay, and using TOOS, MAOS for color reagents, etc., if it is quantitative analysis of very small indicators, it is recommended to use chemiluminescence method , Luminol or acridinium ester can be used as luminescent reagent.

The prosperity during China's National Day is the most powerful signal to the world of our economic recovery. Many places abroad are still dealing with the second rebound of the epidemic, but all walks of life in China have gradually entered the right track. Like carbomer, a raw material for daily chemicals, China, which originally relied on imports, is now self-sufficient and favored by foreign manufacturers.   In terms of the overall economy, the World Bank predicts that China's GDP will grow by 1.6% this year, while the global economy as a whole will shrink by 5.2%. China has achieved a faster recovery through various measures. The effect is obvious. It is estimated that by the end of this year, China's share of global GDP may rise by about 1.1 percentage points, which is more than three times the share obtained in 2019. In contrast, the shares of the United States and Europe both declined slightly. It is estimated that by the end of the year, China’s total economic volume will be approximately US$14.6 trillion, which is approximately 17.5% of global GDP.   Regarding imported raw materials, carbomer raw materials are only one of a variety of raw materials, and there are also many daily chemicals, chemicals, food, reagents and other raw materials that are gradually showing a trend of domestic raw materials replacing imported raw materials. Although some raw materials in Europe and the United States have inherent advantages such as early industrial development and complete industrial chain, some of them are indeed ahead of domestic enterprises in terms of technology and entrepreneurship.   Carbomer raw cardboard drums ready for loading   After the outbreak of the epidemic, the restricted import of raw materials has also accelerated the research and development process of domestic high-quality raw materials, enabling many raw material production technologies including carbomer to gradually develop to the international advanced level. On the other hand, China's large population and high market demand have laid a foundation for the development of domestic reagent raw materials, and can also stimulate the potential of domestic enterprises in the development and innovation of raw materials technology.   The raw materials of carbomer, especially carbomer 980 and 940, due to its high efficiency of gelling, suspending, thickening, and dispersing in many substances without adverse effects on each other, it is used in cosmetics, shampoos, medical The position in products such as accessories is basically irreplaceable. For modern daily life, the demand for carbomer is also very large.   The performance of carbomer used in different industries is also different. In addition to being used as a gel in skin care products and cosmetics, carbomer can also be used as a hand-washing gel, which is also used more during the epidemic. A disinfectant hand sanitizer. The demand for carbomer raw materials has increased and has been recognized by many foreign companies. This also reflects the significant recovery of China's economy.

As a manufacturer, Desheng always considers many aspects of its products. We must not only ensure the integrity of the production process during the production process, without any mistakes, but also ensure that the various indicators of the product during use must reach a satisfactory range after the product is completed.   Serum separation gel is the main product of Desheng. The main sales turnover comes from separation gel, and the investment in separation gel is also the most. Machinery and equipment, production personnel, R&D quality inspection, sales logistics and so on. So the problems encountered are the most.   The first is the purchase of raw materials. A few days ago, I heard that the separation glue we produced had problems and could not be produced normally. I found many problems. Finally, I realized that one of the raw materials did not meet the standard, which led to a series of The problem, we were forced to stop production for a few days for this raw material. Therefore, no matter which part of the production has problems, all efforts may be wasted.   All successes can't come by hand, they are all the experience summed up in the continuous failure. Even after the product is produced, we must pay attention to various problems encountered in use. For example, a series of problems such as the air bubble problem of the serum separation gel, the problem of turning over, the problem of blood clamping, the difficulty of adding gel, the problem of specific gravity, etc., are all we have to find ways to solve. This is only one of our products. Among our dozens or hundreds of products, one can imagine how many problems we will encounter. When customers buy our products, we must guarantee the quality of our customers. But Desheng is not afraid of any problems. What we are afraid of is that there will be no problems. We believe that any problem can be solved, and we also have the confidence to solve the problem. If there is no problem, how can we grow and how to make progress?   Through this hard work, we have grown from a small workshop in 15 years to now have our own factory and office. Our product quality and technology have been widely recognized and used by customers in the Chinese local market. Many well-known domestic blood collection tube manufacturers have been choosing and using our products for many years. Our products have already gone to the world, serving the health and medical causes of people from all over the world.   We have always insisted on our service philosophy: We do not hesitate to take on our problems; Problems that do not belong to us, we do our best to help customers solve; The problem of unclear responsibilities belongs to us.

In recent years, my country's in vitro diagnostic industry has developed rapidly and has become one of the fastest growing markets for IVD in the world. At present, my country has a relatively complete in vitro diagnostic industry chain, including upstream raw material supply links, midstream instrument and reagent R&D, production and sales links, and downstream demand markets. The quality of raw materials, especially core raw materials, directly determines the quality of in vitro diagnostic reagents. This article will introduce the core raw materials of in vitro diagnostic reagents and their importance.   In vitro diagnostic reagents refer to reagents, kits, calibrators, quality control products and other products used for in vitro testing of human samples. The upstream core raw materials include enzymes, serum primers, antigens, antibodies and other biological products, in addition to various fine chemicals Raw materials, such as biological buffers, various amino acids and organic acids, etc.   Among them, enzymes are the most widely used core biological materials, including enzymes, coenzymes, and enzyme substrates, such as horseradish peroxidase, ascorbate oxidase, proteinase K, etc., and are widely used in biochemistry, immunity, molecules, POCT, coagulation, and blood sugar Almost all in vitro diagnostic sub-fields. Antigens and antibodies are one of the most important raw materials for in vitro diagnostic reagents. Antigens include proteins, polysaccharides, polypeptides, lipids, small molecule compounds, etc. The antibodies mainly include monoclonal antibodies and polyclonal antibodies, which are mainly used for enzyme-linked immunoassay and chemiluminescence Detection platforms such as immunoassay, colloidal gold lateral chromatography, fluorescent immunolateral chromatography, biological buffers and other fine chemicals are mainly used in the buffer solution system for preparing reagents. The most used biological buffer is TRIS, which can not only As a buffer, it can also be used in pharmaceutical intermediates and pharmaceutical excipients. In vitro diagnostic reagents with different colors The development and supply of these upstream core raw materials deeply restricts the development of the industry and restricts the development and use of in vitro diagnostic reagent products. The new crown epidemic has fully highlighted the importance of in vitro diagnostic reagents and their raw materials. In the main detection methods of the new coronavirus, nucleic acid detection relies on raw materials such as enzymes and serum primers, while antibody detection mainly relies on raw materials such as antigens and antibodies.   Take the new coronavirus nucleic acid detection as an example. The process includes sample collection and reception, inactivation, nuclear collection registration, reagent preparation, nucleic acid extraction, computer amplification, and result analysis. Virus sampling tubes and RNA extraction are used. Reagents and instruments such as kits, fluorescent quantitative PCR machines, vortex mixers, centrifuges and PCR reaction tubes. The new coronavirus nucleic acid detection kit includes 2X qPCR main premix, reverse transcription premix, PCR primer and probe set, buffer, new coronavirus positive control, negative control and other components. Many core materials are used in the whole process, such as guanidine isothiocyanate, a component of the virus preservation solution in the virus sampling tube, and Tris or Tris-HCL, EDTA, proteinase K, etc. contained in the lysate of the nucleic acid extraction kit.   It is precisely because of the support of these excellent raw materials that the quality of new coronavirus detection products can be guaranteed and they have played an important role in the prevention and control of the epidemic. Therefore, the core raw materials play a very important role in the entire in vitro diagnostic field. It is precisely because Desheng always adheres to the service tenet of quality first and leading technology to ensure all links from raw material procurement to production, so that R&D and production are trusted by customers. The main products currently produced are: biological buffers, chemiluminescence reagents, virus preservation solutions, carbomers, blood collection tube additives and other products. For more information about in vitro diagnostic reagents, please pay attention to Desheng Chemical.

The treatment process of medical waste in medical institutions mainly includes: classified collection, autoclave treatment, hospital transfer, temporary storage, and transfer for centralized disposal. To determine whether a patient suspected of new coronary pneumonia is infected with the new coronavirus from the etiology, and to evaluate the patient's condition, it is necessary to collect samples with nucleic acid detection material virus preservation solution for testing. The tested samples include nasopharyngeal swabs, sputum, blood, urine, etc. Desheng can provide various detection materials for virus preservation solution, nasopharyngeal swabs, and disposable sampling tubes. After completing the test, how do the inspectors handle these virus-containing samples?   Desheng Virus Transport Media and disposable sampling tube   1. Autoclave the specimen After the outbreak of the new crown pneumonia epidemic, Beijing You'an Hospital was designated as a designated hospital to treat patients with confirmed and suspected cases. Researcher Zhao Yan, director of the clinical laboratory of the hospital, said in an interview with a reporter from the Science and Technology Daily: "After the inspectors have completed the sample testing, the medical waste generated is mainly the specimens after the test and the experimental waste generated during the test (such as solid waste samples). Containers, pipette tips, etc.), as well as the waste liquid generated during the experiment of the inspection equipment. The medical waste generated is classified as infectious medical waste according to the "Medical Waste Classification Catalog."   In accordance with the "Medical Waste Management Regulations" and related regulations, for specimens, consumables, laboratory waste liquids and other high-hazard wastes that may contain pathogens in medical waste, laboratory testers need to perform pressure steam sterilization or chemical disinfection in the laboratory . The specimens after the test and the waste consumables in contact with the specimens during the test shall be sterilized by pressure steam. As for the infectious waste liquid, it needs to be soaked in a disinfectant with an effective chlorine content of 0.55%, and after the sterilization effect is verified, it is discharged into the laboratory water treatment system. "It should be noted that pressure steam sterilization is not suitable for all medical wastes. Pressure steam sterilization is mainly suitable for the treatment of infectious and damaging wastes, but not for pathological wastes, chemical wastes and pharmaceutical wastes. "Zhao Yan said.   2. Make registration and centrally store The Clinical Laboratory Center of the Department of Health of Henan Province and the Laboratory Department of the First Affiliated Hospital of Zhengzhou University issued the "New Coronavirus Specimen Laboratory Testing and Biosafety Processing Procedures" (hereinafter referred to as "processing procedures"), which mentioned that special packaging bags for medical waste, There should be warning signs on the outer surface of the sharps box. Before the medical waste is contained, careful inspection should be carried out to ensure that it is free from damage and leakage.   The medical waste collection bucket should be pedal type with cover. Each packaging bag and sharps box should be attached or affixed with a Chinese label. The content of the label includes: medical waste producing unit, producing department, production date, category, and the special instructions should be marked with "new coronavirus infection pneumonia".   In addition, inspectors must strictly register the medical waste after pressure steam sterilization. It is reported that the contents of the registration include the source, type, weight or quantity of the medical waste, the time of handover, the final destination and the signature of the person in charge, with special indication of "new coronavirus-infected pneumonia" or "new crown". The registration information needs to be kept for 3 years. Zhao Yan told the reporter of Science and Technology Daily that the temporary storage of medical waste in medical institutions does not exceed 24 hours, and then it is transferred by special medical waste transportation vehicles to the final medical waste disposal unit for centralized disposal.   3. Prevent virus leakage and ensure biological safety The standard handling of virus samples involves biological safety. In history, there have been incidents in which people were infected due to improper laboratory management, resulting in a virus leak. According to my country's relevant regulations, the new crown virus belongs to the definition of highly pathogenic pathogenic microorganisms. Article 33 of the "Regulations" clearly states that the establishment of laboratories engaged in experimental activities related to highly pathogenic pathogenic microorganisms should establish a sound security system and adopt security measures to prevent the theft and theft of highly pathogenic pathogenic microorganisms. Being robbed, lost, or leaked to ensure the safety of the laboratory and its pathogenic microorganisms.   If the pathogenic microorganism laboratory leaks highly pathogenic microorganisms, the laboratory staff shall immediately take control measures to prevent the spread of highly pathogenic microorganisms, and report to the institution or personnel responsible for laboratory infection control.

Luminol is abbreviated as luminol, which is a commonly used chemiluminescence reagent. It can emit a light blue glow when oxidized by an oxidant. It is usually used to detect blood stains in criminal investigations or used with POD for biochemical detection of chemiluminescence equipment.   Some people find that the luminous efficiency is not high when experimenting with luminol. What is the reason? Usually luminol is oxidized to emit light, and the effect is very obvious. If there is flashing or weak luminescence or no luminescence, there may be several reasons: 1. Luminol reagent problem Luminol is a luminescent reagent and is very sensitive to light, so it is usually stored in an opaque brown bottle sealed at low temperature. If luminol expires or has been partially oxidized, the luminous efficiency will be reduced or no light will be emitted.   2. Low peroxidase activity   When luminol is used in biochemical detection, it is usually oxidized with hydrogen peroxide, which needs to be catalyzed by peroxidase POD. If the enzyme activity is low, it will affect the oxidative luminescence performance of luminol. Enzyme catalytic reaction requires high temperature and pH value. Too much sodium hydroxide increases alkalinity or impurities in the system will affect enzyme activity.   Third, the concentration of the excitation solution and the proportion of ingredients The excitation liquid used for luminol luminescence usually refers to hydrogen peroxide solution and sodium hydroxide solution. When used in different sample detection tests, there are certain differences in the concentration of materials. The proportion of ingredients and the order of reagent addition are all related to the entire chemical The luminescence experiment has an impact, this needs to be adjusted for a specific experiment.   Some luminol chemiluminescence experiments were not completed under the catalysis of peroxidase, but potassium ferricyanide, ferric chloride, ferrous sulfate, potassium dioxalate ferrite, etc. were used as catalysts to catalyze the oxidation of H2O2. Mino. When using this type of catalyst, pay attention to the iron ion concentration not too high, otherwise luminol will easily cause instant flashing.   If the chemiluminescence effect of luminol is not good, it is mainly due to the above reasons. Desheng is a manufacturer of chemiluminescent reagents such as luminol and acridinium esters. According to experience, novices who buy luminol reagents for experiments should do a few more regular tests based on the above circumstances to cause the problem early.