China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Recently, I always receive customers' inquiries about Good's buffers, but many times even the customers themselves are not able to express their exact products exactly. Because there are still a few people who really understand, I will briefly introduce Good's buffer and the difference between PIPES and HEPES in Good's buffer.     Good's buffers, also known as zwitterionic buffers, are a type of buffer system dedicated to life science research. They do not participate in or interfere with the biochemical reaction process, and have no inhibitory effect on enzyme chemical reactions. Therefore, they are specifically used for organelles and electrodes. Research work on volatile, pH-sensitive proteins and enzymes. These buffer systems should have the following characteristics:   1. The pKa value is between 6-8;   2. High solubility in water;   3. Not easy to penetrate biofilm;   4. Little salt effect;   5. The ion concentration, solution composition and temperature have little effect on dissociation;   6. No complex or precipitation with metal ions;   7. The buffer is chemically stable;   8. Small absorption of light in the ultraviolet and visible wavelength range;   9. Easily produce high-purity salt.   The PIPES buffer and HEPES buffer in Good's buffer are our commonly used buffers, and they are inextricably linked. To a certain extent, they have the function of acquaintance, but they also have their own functions and advantages. After we understand Good's buffer, let’s take a look at PIPES and HEPES, let us slowly penetrate into their respective fields, so that we can be more Good choices use their respective characteristics:   PIPES   The pH buffer range is 6.1-7.5, insoluble in water, and soluble in aqueous NaOH solution. PIPES is different from buffers containing bis(2-hydroxyethyl)amino groups (such as Bis-tris, Bicine), and cannot form stable complexes with most metal ions. It is suitable for buffers in solution systems containing metal ions. According to the existing research results, PIPES can be applied to the purification of tubulin using phosphocellulose chromatography, the purification of recombinant GTP-binding proteins ARF1 and ARF2 by gel filtration, and as a buffer to crystallize transketolase from E. coli. In addition, because PIPES can form free radicals, it is not suitable for use in redox systems. In cation exchange chromatography, a low concentration of PIPES buffer should be used, because PIPES has a relatively large ionic strength, and its pKa value is concentration-dependent.    HEPES   The pH buffer range is 6.8-8.2. It is soluble in water. It is a hydrogen ion buffer that can control a constant pH range for a longer period of time. The final concentration is 10-50mmol/L, and the general culture medium contains 20mmol/L HEPES to achieve buffering capacity. It does not form stable complexes with metal ions, and in most cases, does not interfere with biochemical processes. HEPES is commonly used in cell culture media of various types of organisms; in protein research, PIPES is often used as a combination in cation exchange chromatography Buffer components and eluent; reaction buffer, pre-hybridization buffer, hybridization buffer for separation and analysis of RNA nuclear components; 3′-terminal labeling for RNA and T4RNA ligase; used in biochemical diagnostic reagents In the kit, DNA/RNA extraction kit and PCR diagnostic kit. In DNA research, PIPES is used as a buffer for calcium phosphate and DNA precipitate formation systems, AFM and buffer for electroporation experiments. In addition, HEPES interferes with the reaction between DNA and restriction enzymes, and is not suitable for Lowry's method to determine protein content.   It can be seen that neither PIPES nor HEPES can form stable complexes with metal ions, which is suitable for solution systems containing metal ions. However, there is also a certain difference between them. In terms of solubility, PIPES is insoluble in water, while HEPES buffer has good water solubility; in terms of buffer range, PIPES is acidic to neutral, and HEPES is neutral to alkaline. These are the same and different All tell us that to choose them better, we must first understand them. Desheng already has extensive experience in Good's buffer. It provides you with technology products, teaches you how to choose the right choice to distinguish what you need. Why don't you choose such a company!

4-Hydroxyethylpiperazineethanesulfonic acid, referred to as HEPES, CAS No. 7365-45-9, is usually used as a biological buffer in biochemical experiments. It has properties similar to EPPS and is commonly used for pH-sensitive proteins and enzymes. research work. Physical and chemical properties: HEPES Molecular formula: C8H18N2O4S Molecular weight: 238.31 Status: crystalline powder pKa:7.45-7.65 Buffer range: 6.8-8.2 Structure: Purity: greater than 99% Use concentration: 10-50mmol/L   Depending on the application, HEPES buffers are subject to two commonly used buffer systems: HEPES buffer solution: HEPES + NaOH (500mL): 119.15g HEPES is dissolved in 400mL distilled water, add 0.5~1M NaOH aqueous solution to adjust at least the required pH, pay attention to the effective pH buffer range is 6.8-8.2, and then use distilled water to make volume To 500mL; 2. HEPES buffered salt solution: HEPES 6.5g, NaCl 8.0g, Na2HPO4·7H2O 0.198g, adjust the pH value with 0.5M NaOH aqueous solution, and make the volume to 500mL. 3.2×HEPES buffered salt solution: Dissolve 1.6g of NaCl, 0.074g of KCl, 0.027g of Na2HPO4.2H2O, 0.2g of glucan or dextran and 1g of HEPES in 90ml of distilled water, and adjust to the required pH with 0.5M of NaOH Value, then dilute to 100ml with distilled water. In the cell-cell adhesion experiment, it contains calcium and magnesium ions; HA cell culture solution does not contain calcium and magnesium ions, but contains BSA.   The advantages of HEPES buffer: 1. Unlike PEcine, HEPES does not contain coordination groups and cannot form stable complexes with most metal ions. It is suitable for buffers in solution systems containing metal ions. 2. HEPES has very good water solubility, and the buffer range is close to neutral. Although PIPES does not form a complex with most metal ions, PIPES can form free radicals, so it is not suitable for redox systems and has relatively large ions. Strength, PIPES is more acidic. 3. HEPES has no toxic and side effects on cells at low concentration, and can control a constant pH range for a long time. The final concentration is 10-50mmol/L, and the general culture medium contains 20mmol/L HEPES to achieve buffering capacity. Therefore, it is commonly used in HA solution, cell culture solution, virus preservation solution and even skin care products.   Among the biological buffers, EPPS and PIPES are similar in properties to HEPES. They are used as buffers for different experiments, and sometimes can be replaced with each other. Desheng is a manufacturer of buffers. It has more experience in the production and sales of various buffers. Partners are welcome to visit and guide!

Cyclohexylpropanesulfonic acid CAPS, CAS No. 1135-40-6, is an important chemical raw material. It is usually used as a biological buffer in biochemical experiments to maintain the pH of the reaction system. There are many types of biological buffers, so which experiments can CAPS be used for? This requires first understanding how to choose a buffer.   1.Selection of buffer pH range: The buffering range of the buffering agent must first conform to the pH value of the reaction system, such as the reaction condition pH value, protein activity, or enzyme catalyst pH value. The buffer range of the buffer depends on its ionization equilibrium Changshu pKa value. The pH value of the buffer solution is related to the ionization equilibrium constant of the acid and the concentration of salt and acid. The formula for calculating the pH value of the solution is: pH=pKa-lg(Na/Nb ), Na and Nb respectively represent the amount of conjugated acid and alkaline substance. The buffer range of the buffer is between the plus and minus 1 of the negative logarithm of its power balance constant, and pH=pKa±1. The pKa of CAPS is equal to 10.4, the theoretical buffer range is 9.4-11.4, in order to ensure accuracy in biochemical experiments The upper and lower limits are reduced by 0.3 to use 9.7-11.7, so the pH of the experimental system that can use CAPS as a buffer must be in this weakly alkaline pH range. Common buffer buffer range   2. the ionization balance of commonly used buffers Changshu and buffer range In many reactions such as complexometric titration, spectrophotometry, and enzymatic reaction, the pH of the solution is required to be kept within a range to ensure the color change of the indicator, the color development of the color reagent, and the optimal pH value for enzyme catalysis, etc. These conditions are achieved by adding a certain amount of buffer solution, so the buffer solution is a reagent often required in analytical tests.   Using the potentiometric titration method to determine the titration curve of added acid or alkali to the same buffer solution with different ratios, not only helps to understand the concept of buffer solution and buffer capacity (range) but also to correctly select the preparation method and dosage of buffer solution in analytical testing Instructive. It can be seen from the chart that CAPS is higher among buffers and belongs to weak alkaline buffer.   3. Restrictions on the use of buffers In the case where the buffer range meets the reaction system, it is also necessary to consider the limiting conditions of the reaction system, for example, phosphate buffer will complex metal ions calcium, enzymes, etc., and the activities of enzymes and proteins in some reactions are affected by metal ions. Phosphate buffer cannot be used. CAPS buffer is suitable for the electrophoresis buffer of high molecular weight proteins (greater than 20KD); if it is a low molecular weight protein blotting experiment, Tris + glycine is usually used, and Tris-Tricine + EDTA is used to exclude glycine for protein sequencing.   In addition to being used as a biological buffer, CAPS reagents are also commonly used in waterborne coatings and other industries. Desheng has extensive experience in the production and development of cyclohexylamine propanesulfonic acid and other various biological buffers, which is worthy of the majority Customer trusted partner!

Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid or TAPS, CAS: 29915-38-6, is a zwitterionic buffer, widely used in the field of biochemistry and molecular biology, usually white crystals or Powder is a Good's biological buffer mainly produced by the company.   In clinical diagnostic biochemical testing, TAPS is mainly used as a biological buffer. Its molecular structure contains a sulfonic acid group and an amino group substituted by a polyhydric alcohol, the sulfonic acid group is weakly acidic, and the amino group is weakly basic, so as to maintain the pH value of the reaction system, the buffer range is 7.7-9.1; Good, the solubility can reach 50g per 100g of water; therefore, it is often used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits.   Tris(hydroxymethyl)methylaminopropanesulfonic acid TAPS   In addition to the kit, TAPS is also used to protect oxyhemoglobin during freeze-drying, as a protective agent to prevent the oxidation of hemoglobin to methemoglobin. This is very important, because traditional blood transfusions have many drawbacks, such as blood-borne infectious diseases, complicated blood type matching, and viral infections. Hemoglobin oxygen carriers can be used as good blood substitutes. However, hemoglobin is easily oxidized to methemoglobin without oxygen carrying capacity during the freeze-drying process, so it is necessary to add a protective agent to prevent hemoglobin degeneration, TAPS is one of the important components.   At present, a domestic TAPS synthesis method is: Tris methylaminomethane Tris and 1,3-propane sultone (1,3-PS) in n-butanol solvent, Tris amino nitrogen atom and Hydrogen atoms are added to the carbon and oxygen in which propane sultone is broken and ring-opened to form TAPS. In this reaction, n-butanol can be recycled to improve product yield and purity.   TAPS, a buffer used in biochemical testing and molecular diagnostics, has very high requirements on reagent purity and impurity ion content. Desheng Technology has done a lot of research and improvement in this area, and many biological buffers produced by the company have obtained a large number of Recognized by biochemical testing companies and medical device companies.

HEPES is an important biochemical preparation in biochemical industry. It is considered to be one of the important buffers in the field of biological research. The chemical name of HEPES is: N-hydroxyethylpiperazine-1-ethanesulfonic acid, the character is white crystalline powder , Is a weak acid, often used as zwitterionic buffer and pharmaceutical intermediate in organic chemical industry. It is often selected as a buffer for cell culture because of its advantages:   Desheng's location and buffer products   1. The decomposition capacity of HEPES can be enhanced with the increase of temperature and weakened with the decrease of temperature, but compared with other buffers, its decomposition constant does not change much with temperature, which makes HEPES buffer become A buffer that better maintains the structure and function of the enzyme. The buffer can control a constant pH range for a longer period of time and has no toxic effect on cells.   2. The pH required for most cells is 7.2-7.4, HEPES has a good buffer capacity in this range, but the optimal pH value varies with the type of cell cultured. Fibroblasts prefer higher pH (7.4-7.7), while subcultured cell lines require acidic pH (7.0-7.4). Most culture medium relies on sodium bicarbonate (NaHCO3) and CO2 system for buffering. The CO2 concentration in the gas phase should be balanced with the sodium bicarbonate concentration in the culture medium. In this case, the cell culture bottle cap should not be screwed too tight to ensure gas exchange.   However, after storage for a certain period of time, the pH of the buffer system will increase with the CO2 volatilization. At this time, the culture solution quickly becomes purple when the cell is subcultured and blown, making the cells cultured with this culture medium difficult to survive. Even if it survives, the activity It is also very low, and the proliferation rate is very slow. Adding HEPES to the culture solution can avoid this problem. HEPES can be used as a buffer to replace bicarbonate to eliminate the limitation of the high-concentration CO2 culture environment. The final concentration of HEPES buffer used is generally 10-25mM.   How to use HEPES:   1. HEPES can be directly added to the prepared culture solution at the required concentration, and then sterilized by filtration. Add 2.38 grams of HEPES per 1000ml of culture solution, adjust the pH to 7.2 with lN NaOH after dissolution, filter and sterilize and use At this time, the use concentration of HEPES is 10 mmol/L.   2. It can also be prepared into 100 x storage solution (l mol/L). Before use, 99ml of culture solution is added to lml storage solution, and the final application concentration is still 10mmol/L. l mol/L (100 x) HEPES stock solution preparation method: dissolve 23.8g HEPES in 90ml double-distilled water, adjust pH to 7.5-8.0 with 1N NaOH, then dilute to 100ml with water, filter and sterilize, and dispense vials ( 2ml/bottle), store at 4℃ or -20℃.   Desheng Biochemical reminded that when used as a buffer for cell culture, HEPES in the culture medium exposed to visible light for more than 3h will produce hydrogen peroxide that is toxic to the cells. It is recommended that the culture medium be stored in the dark.

With the rapid development of China's economy in the 21st century, people's living conditions have made a qualitative leap compared to the 20th century. However, while the material conditions are extremely rich, due to excess nutrition, lack of exercise and other reasons, rich diseases such as diabetes, hypertension and hyperlipidemia have also begun to spread. In order to better and more convenient for people to monitor and diagnose these diseases, a variety of diagnostic and therapeutic instruments that can be used for bedside diagnosis, such as blood glucose meters, multiple lipid test cards, and triglyceride test strips, have been developed in succession. Today we are going to talk about MAOS is a core material that can be used with blood glucose test paper, total cholesterol test paper and the other two test papers mentioned above.   The maximum absorption wavelength and molar absorbance of the new Trinder’s reagent   MAOS(CASNo.:82692-97-5)full name: N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt monohydrate, is a new Trinder's A very important member of the reagent family. It is not as commonly used as TOOS, but it also has an absolutely irreplaceable role. As can be seen from the figure below, the maximum absorption wavelength of MAOS is 630nm, which has a larger absorption wavelength than other Trinder’s reagents. Since Trinder's reaction principle is the hydrogen peroxide produced by the tested substance under the action of the enzyme, then 4-aminotepyrine and catalase oxidize the chromogen substance to a red quinoid substance, and then use light absorption method to determine the Measured substance concentration.   The scope of application of MAOS and other Trinder's reagents is almost the same, between PH 5.0 and 9.5, but the maximum absorption wavelength of MAOS makes it can greatly reduce the interference of other components in the sample to be tested, so it is widely used in the need for relatively accurate Numerical applications such as blood glucose test strips.   MAOS product description Product Chinese name N-乙基-N-（2-羟基-3-磺丙基）-3,5-二甲基苯胺钠盐一水合物 English name N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt monohydrate CAS　No. 82692-97-5 Customs code 2922199090 Molecular formula C13H20 NNaO4S, H2O Molecular formula 327.37 Exterior White or light yellow pink powder Storage conditions Room temperature（20-25℃），Protect from light and moisture Transport conditions Room temperature（20-25℃） Maximum absorption wavelength 630 nm Molar absorbance (pH10) ≥10,000 （about 257 nm） Oxidation reaction application Hydrogen peroxide reaction, colorimetric method   Desheng's MAOS has the characteristics of high purity, good crystal form and pure white color. In addition, MAOS produced by Desheng has also passed the verification of the five major domestic biochemical diagnostic reagent giants, and more than 100 companies have selected Desheng as their reliable supplier. Welcome to consult!

The full name of TOPS (CAS No.: 40567-80-4) is N-Ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt, which is a white to light brown powder, also A member of the new Trinder's reagent family. Maybe you think the name sounds humongous, and it is a vocabulary that a fully professional pharmacist only understands. It is even more difficult to relate to your life. If you think so, you are wrong. You may not have heard of this name, but I think most of you or people around you have done biochemical tests in the hospital, that is, liver function, kidney function, uric acid, cholesterol and other tests. Most of these tests are performed by automatic biochemical analyzers, uric acid analyzers, etc. in conjunction with the corresponding kits. And one of the core materials in these kits is the TOPS we talk about today.   Desheng TOPS reagent   In humans and primates, uric acid is the final product of purine metabolism. It is produced by the oxidation of xanthine and hypoxanthine by xanthine oxidase and is excreted in the urine. High serum uric acid and hyperuricemia are associated with insulin resistance, cardiovascular disease and gout. The mechanism leading to hyperuricemia is usually increased uric acid production or decreased urine excretion. Increased serum uric acid may be a sign of kidney disease, so the early diagnosis of kidney disease can be indirectly through the test of uric acid.   TOPS product description Product Chinese name N-乙基-N-(3-磺丙基)-3-甲基苯胺钠盐 English name Sodium 3-(N-ethyl-3-methylanilino)propanesulfonate CAS No. 40567-80-4 Customs code 2922199090 Molecular formula C12H18NNaO3S, H2O Molecular formula 297.34 Exterior White or light brown powder Storage conditions 0-5℃，Protect from light and moisture Transport conditions Room temperature（20-25℃） Maximum absorption wavelength 550 nm Oxidation reaction application For colorimetric determination of uric acid and cholesterol. Good water solubility, high sensitivity and strong stability. Cholesterol colorimetric determination; water-soluble reagent, used for catalase photometric determination     In the presence of hydrogen peroxide and peroxidase, TOPS reagent is formed during the oxidative coupling reaction with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazolone hydrazone (MBTH) Very stable purple or blue dye. The substrate is enzymatically oxidized by its oxidase to produce hydrogen peroxide. The concentration of hydrogen peroxide corresponds to the substrate concentration. Therefore, the amount of substrate can be determined by the color of the oxidative coupling reaction to obtain a relatively accurate test result of the concentration of the analyte. TOPS produced by Desheng has obtained the quality certification of over 100+ manufacturers nationwide. Because of its color rendering sensitivity, color rendering stability and anti-interference, as well as the accuracy and precision of specific measurement items, it has been well received by customers. You are welcome to inquire, the company will provide you with quality products and excellent services, so that your products rank among the industry's leading level!

TOOS is also called EHSPT (N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt), is a highly water-soluble sodium salt of aniline, the sensitivity of the Trinder reaction It is much higher than traditional phenol, aniline, ADA and other color-developing substrates. Clinical biochemical testing is often used in liver function kits, blood glucose metabolism kits, etc.   In the presence of hydrogen peroxide and peroxidase POD, Trinder's reagent forms a very stable orange or red quinone with 4-aminoantipyrine (4-AAP). The molar absorbance of TOOS and MBTH coupling dye is 1.5-2 times higher than that of 4-AA coupling dye; however, 4-AAP solution is more stable than MBTH solution, and Trinder’s reagent is more used with 4-AAP.   Color substrate reagent TOOS   When tested with a biochemical kit, the measured indicators such as uric acid, glucose, glycated albumin, and 1,5-anhydroglucitol are equal to the enzymatic oxidation of its oxidase to produce hydrogen peroxide. The concentration of hydrogen peroxide corresponds to the concentration of the test substance Therefore, the amount of the analyte index can be determined by the color development of the oxidative coupling reaction. Of course, enzyme activity indicators can also be measured, such as adenosine dehydrogenase detection kit and 5'-nucleotidase detection in liver function series. It is through the reaction of the enzyme to be detected and its corresponding catalytic substance to generate hydrogen peroxide, and then through the coloring substrate TOOS coupling 4-AAP to react with the generated hydrogen peroxide, the final rate of production of the coloring product corresponds to the measured Enzyme activity, so as to achieve the purpose of measuring indicators.   The TOOS color development substrate developed by Desheng Technology has the characteristics of high purity, high water solubility, and low interference impurity ions. It can be quickly configured during use, which is very convenient; and the company also produces Tris buffer used with it for biochemical detection. Wait, the quality and service are well received!

The full name of ethylenediaminetetraacetic acid tripotassium tripotassium ethylenediaminetetraacetic acid tripotassium, English abbreviation: EDTA K3, is a white crystalline powder, which is colorless, odorless, easily soluble in water, and very easy to absorb moisture. Although it looks unremarkable, it is an application and a wide range of chemical raw materials. Today we will analyze the application and characteristics of tripotassium EDTA in various aspects.   Photo of EDTA .K3     CONTENT SPECIFICATION 1 Molecular weight 442.6 2 Appearance White amorphous powder, easy to absorb moisture 3 Main content ≥99.0 4 pH value 7.5±1 5 Clarity Transparent 6 Solubility（%） ≥60.0 7 chloride(Cl),% ≤0.005 8 Sulfate (SO4), % ≤0.02 9 Iron (Fe) ,% ≤0.001 10 Heavy metals (eg lead pb),% ≤0.001   EDTA K3 test report   1. It is used in the preparation of Anticoagulant For Blood Collection in medical tests, purple anticoagulation tubes, vacuum anticoagulation tubes, and a whole blood analyzer. It is especially suitable for blood tests in various clinical emergency departments, and is also an important additive in blood collection tubes. As a EDTA salt in the industry, Hubei Xindesheng Material Technology Co., Ltd. can protect the blood cell components, does not affect the white blood cell count and size, has minimal impact on the morphology of red blood cells, and can inhibit platelet aggregation, suitable for general hematology tests. Except for the separation test of platelets, it is not suitable for coagulation test and platelet kinetic energy test, nor for calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine aminopeptidase Determination and PCR experiments.   2.Used in detergents, liquid soaps, shampoos, agrochemical sprays, antidotes. Ethylenediaminetetraacetic acid in EDTA K3 is an amino polyacid. Because it can form a chelate with most metal ions, it becomes a general-purpose strong chelating agent. Due to this property, it can be combined with deionized water, solid alkali, and linear alkylbenzene sulfonic acid, surfactants and some auxiliary agents to form a detergent, and this formula is non-toxic to the skin. Harmful, non-irritating. And as a detergent, it can exert its chelating effect, mainly chelating with calcium and magnesium ions, so that the water is softened and easy to clean.   3. A self-cleaning high-borosilicate glass rod is prepared. It can be first mixed with deionized water, 34-44 parts of aluminum isopropoxide, etc. and heated to 40-45°C to form a colloidal liquid, and then reacted with tetrabutyl titanate, 11-15% ammonia water, ethanol to generate After the colloidal liquid is mixed and stirred evenly, a self-cleaning coating is made. After the surface of the high borosilicate glass rod is cleaned, it is placed in this self-cleaning coating for 10-14 minutes, then taken out and placed in a high-temperature furnace. After sintering, the finished high-borosilicate glass rod with self-cleaning function can be prepared. In the preparation process, the tripotassium salt of ethylenediaminetetraacetic acid is used as an important additive in the colloidal liquid, which can effectively improve the adhesion performance of the prepared colloidal liquid, improve the uniformity of the dispersion of the AlOOH colloid, and prevent its precipitation. The phenomenon becomes an important colloidal dispersant.   4. A heat insulation coating for building exterior wall is prepared. In terms of parts by weight, including pure propylene emulsion 80-120 parts, Ta2O5-ZnO-SnO2 composite oxide powder 20-30 parts, ethylenediaminetetraacetic acid tripotassium 5-10 parts, kaolin 2-5 parts, borax 1-5 Parts, antifreeze 2 to 5 parts, film-forming aid 5 to 10 parts, pyrophosphate 1.5 to 2.3 parts, thickener 1 to 2 parts, polyoxyethylene polyoxypropylene pentaerythritol ether 2 to 3 parts and o-nitro Benzenesulfonic acid 0.5 to 1 part, water 100 to 150 parts. Through the test, it was found that the introduction of tripotassium ethylenediaminetetraacetic acid in the exterior wall paint can significantly improve the acid corrosion resistance of the paint, so that in cities with severe acid rain, the above paint can also ensure a good service life and is not easy to corrode.   5. Preparation of dyes. SDS, bromophenol blue, EDTA K3, sucrose, etc. can be used to make a 2.5-fold concentrated protein loading buffer. Take an appropriate amount of protein sample and dye mixture, mix well, and then directly load the sample into the relevant gel sample hole to perform relevant detection and operation.    

Mentioned that war epidemic technology has to mention that the important means to block the spread of the epidemic is nucleic acid detection. The raw material of the detection reagent is the key to the role of nucleic acid detection, and this core raw material is the Virus Transport Media. Many people will feel frightened and helpless when it comes to new coronary pneumonia. It is extremely contagious, leading to isolation for several months in 2020. During the quarantine period, it was also reported that the inspector of the laboratory of Wuhan Jinyintan Hospital was infected with the new coronavirus without contacting the patient. Why is this? Is there any way to solve it?   Virus Transport Media (inactivated and non-inactivated)   The inspectors at the Jinyintan Laboratory never contacted the patients, but only performed tests, Four of them were still infected.How did they get infected? Experts speculate that one possibility is that when performing high-risk operations such as laboratory tests, the patient's blood samples are exposed to the air to form an aerosol, and the four inspectors are carried by the aerosol Infected by a virus. If this is the case, then the virus is very powerful. Without contact with the patient, a little blood is outside and it may become an aerosol. The transmission routes can therefore be divided into contact transmission, droplet transmission, and air transmission, which may account for the main transmission routes. In response to this problem, Desheng has developed a virus inactivation Virus Transport Media, which greatly reduces the possibility of infection during the detection process.   The principle of nucleic acid detection of New Coronavirus is to expose the RNA of the virus through cell lysate, and then use real-time fluorescent RT-PCR for detection. Because the new coronavirus is very infectious, in order to protect the detection personnel and reduce the risk of infection, the virus needs to be inactivated.   Virus inactivation is to make the viral protein no longer have physiological activity, and lose the ability of infection, disease and reproduction. The main method is to inactivate the water bath at high temperature, that is, put the sample in the water bath box and inactivate it at 56℃ for half an hour. In addition, some nucleic acid detection kits come with virus inactivation Virus Transport Media, which can "kill" the virus and protect the RNA during the sample collection stage. This Virus Transport Media is prepared based on nucleic acid extraction lysate.   Because it is based on the preparation of accounting lysate, the role of guanidine hydrochloride, EDTA, TRIS and other reagents in this Virus Transport Media is to cleave the virus to release nucleic acids. Guanidine hydrochloride not only rapidly destroys the cell membrane, but also denatures the protein, allowing the protein to denature and precipitate, allowing the nucleic acid to get rid of the protein. EDTA chelating agent can combine with metal ions such as Mg2+, Ca2+, Mn2+, Fe2+, etc., and reduce the influence of metal ions on nucleic acid quality. On the one hand, the virus inactivation Virus Transport Media directly cleaves the virus to release nucleic acid, and eliminates nucleic acid degrading enzyme (RNase) to prevent the degradation of virus RNA. On the other hand, the protein of the virus can be denatured, lose its activity and "dead", no longer infectious, and improve the safety of the transportation and detection stage.   In addition to the above-mentioned virus-inactivated Virus Transport Medias, Desheng also has non-inactivated Virus Transport Medias. There are also differences in the types of Virus Transport Medias used according to various testing needs. Desheng has been working hard to make its own slight contribution to the prevention and control of the epidemic, hoping that the motherland can prosper safely.

The main properties of Lithium Heparin: a white amorphous powder, odorless, easily soluble in water, easy to absorb moisture, molecular weight 15000. Desheng's lithium heparin product titer ≥150IU/mg, anhydrous titer ≥160IU/mg. For other indicators, please refer to the heparin sodium API standard for control. Desheng's lithium heparin is suitable for the collection and anticoagulation of blood samples in clinical biochemical and emergency biochemical tests, as well as the collection and anticoagulation of blood samples in some hemorheology projects. It is recommended to use lithium heparin as an anticoagulant in clinical tests to determine the content of ions in blood, because it is the least likely to interfere with the determination of other ions.   Lithium Heparin   The condition of emergency patients is usually acute and changes rapidly, and the time of outpatient inspection is sometimes not timely, which will lead to the occurrence of doctor-patient disputes and delay the best time for treatment. In recent years, test reagents have been gradually commercialized and standardized. The application of automatic biochemical analyzers has also been popularized, and the timeliness and accuracy of clinical tests have been improved. However, it is still the goal pursued by clinical laboratory technicians to make scientific and accurate biochemical test results for emergency patients better and faster under the existing equipment conditions.   The test specimens of clinical biochemical indicators are mostly from venous serum, and the clinical reference indicators are also derived from serological standards. However, traditional serological examinations, on the one hand, have slow blood clotting, which can easily delay the treatment of emergency patients. On the other hand, some fibrin remaining in the serum after blood clotting can easily block the needles and tubes of the analytical instrument, causing adverse consequences. In the process of blood coagulation, some of the intracellular substances, such as potassium ions, are precipitated into the serum due to the destruction of blood cells, which results in a high serological measurement value and forms interference.   Therefore, many hospitals have begun to use heparin anticoagulation to analyze the plasma of specimens. The main advantages are that heparin inhibits the adhesion and aggregation of platelets, strengthens the inactivation of thrombin, and strengthens the role of antithrombin III. To prevent blood clotting, plasma can be obtained for analysis as soon as possible; at the same time, anticoagulation using lithium heparin has stronger anticoagulation than traditional heparin sodium, plasma centrifugation speed is faster, and various indicators are closer to serological indicators after analysis advantage.   Notes on lithium heparin anticoagulant: 1. In order to ensure adequate anticoagulant for blood collection, the heparin salt test tube must be reversed and mixed as soon as possible 5-8 times, especially when the blood collection temperature is higher than 25 ℃, the blood and lithium heparin must be mixed in time, otherwise it may lead to Blood clotting or partial clotting.   2. Heparin anticoagulation is a non-irreversible anticoagulation, so the test should be completed within 6h after the blood collection of the lithium heparin anticoagulation test tube, otherwise it may cause errors in the test results.   3. Heparin may participate in the metabolism of cellular enzymes and ions, so the amount of heparin may affect the test results. The dosage of heparin should ensure that the specimen is fully and partially anticoagulated, so that most of the plasma indicators can be repeated within 6 hours, especially sensitive indicators such as AST, ALT, TBIL, DBIL, and GGT.   4. Lithium heparin can be used at the same time as the separation gel. The anticoagulation effect, tube wall siliconization, centrifugal conditions, separation gel quality, etc. will affect the blood separation effect. It is recommended to use with the blood separation gel produced by our company to obtain high-quality plasma samples.   5. Recommendation for irradiation sterilization after adding to blood collection tube: use γ-ray irradiation, the dosage is 8-25kGy. The irradiation dose can be determined by the initial number of colonies.   Hubei New Desheng Material Technology Co., Ltd. is a 14-year-old company specializing in the development and production of blood collection tube additives. We not only do products, but also quality and service. We pursue the long-term of the enterprise Development, only on the basis of good product quality and good service for each customer, is the way of survival and development of the enterprise, customer-oriented, symbiosis and win-win.

The chromogenic substrate MAOS reagent is a high-sensitivity chromogenic reagent commonly used in biochemical kits. The full name is N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium Salt monohydrate; TMB is also a commonly used color reagent. The color development principles of the two are similar, but there are some differences.                                                                                      Trinder Reagent MAOS    MAOS: The reagent belongs to a new type of Trinder's reagent. In the presence of hydrogen peroxide and peroxidase POD, it forms a very stable orange or red quinone with 4-aminoantipyrine (4-AAP). It can be oxidatively coupled with 3-methylbenzothiazole sulfone hydrazone (MBTH) to form a very stable purple or blue dye. The molar absorbance of MBTH coupled dye is 1.5-2 times higher than that of 4-AA coupled dye; however, 4-AAP solution is more stable than MBTH solution, so Trinder's reagent is more used with 4-AAP.   TMB reagent: It is a chromogenic substrate used in ELISA kits. TMB or TMBZ under the catalysis of peroxidase is blue after being oxidized by hydrogen peroxide, and turns yellow after adding stop solution. The OD value was measured with a microplate reader at a wavelength of 450 nm, and the antigen concentration was proportional to the OD value, and the concentration of antigen in the sample was calculated by drawing a standard curve.   It does not need to be like chromogens ADPS, TOOS, MAOS, etc. It needs to add another compound (such as 4-AA, MBTH) to react to show color, but TMBZ needs to react under acidic conditions (HCl) for color reaction. Some biochemical tests are more accurate under neutral conditions. The catalytic performance of some enzymes is very sensitive to pH, and it is necessary to maintain a neutral environment to maintain activity, which limits the application of TMB.   Like other Trinder reagents, MAOS is a highly water-soluble sodium salt of aniline. When coupled with 4-aap, the N of aniline becomes a C=N double bond, and the para position is combined with the amino group of 4-AA to form a C=N double The bond, thus forming a quinoneimine structure, is similar to the C=O double bond of p-benzoquinone, and the absorption wavelengths are in the visible light region, resulting in a color reaction.   The maximum absorption wavelength of the oxidized product of MAOS is far more than that of ordinary color reagents, and its product UV absorption wavelength is quite high at 630nm. If you use a product like MAOS, the maximum absorption wavelength of the product is in the ultraviolet region and is relatively high, which can greatly reduce the interference of substances with similar absorption wavelengths in the sample. Therefore, it is recommended to use MAOS as the color development substrate for some biochemical detection items that require highly accurate values.   All in all, the difference between MAOS and TMB is that the absorption wavelength of the color product is much higher than that of TMB. The coupling requires the introduction of 4-AAP or MBTH, which can be detected in a neutral pH environment. Desheng currently produces 9 new Trinder’s reagents including MAOS.