China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Just yesterday, the National Health Commission issued an announcement, which was madly reposted in the circle of friends because of the sentence "nucleic acid must be extracted for dilution and mixed sample detection, and the "one-step method" is prohibited.   From a technical point of view, there is nothing wrong with this sentence. The one-step method mostly uses direct lysis, and then amplifies after centrifugation or without centrifugation. The reason why this method cannot be used for mixed sample detection is also mixed sample The reason why the test was dissed by many examiners at the beginning, mixing multiple specimens means that the sample is diluted, and dilution also means that there is a risk of missed testing. To   However, as many places in the country began to carry out large-scale nucleic acid screening, a large number of samples followed, and then mixed sample testing was once again put on the discussion table. In disease control, blood stations, etc., blood samples were mixed. In fact, it is a relatively common method, but the blood sample is a homogeneous sample after all, and the new crown is a non-homogeneous swab sample, which is also the culprit of the current epidemic. Can a mixed sample of the new crown work?                                               I don’t know if you have read this document of the Health Commission carefully. In this document, the recommended number of mixed samples is 5, each 200μl, which adds up to exactly 1mL. I think this is why it is recommended to mix 5 with 1 The reason is that the mixed liquid volume is too large or the single volume is too small. Don't say that it can be enriched by centrifugation, this is a virus, and no one can be centrifuged at a speed of tens of thousands of speeds.   This version of the technical guidelines for mixed sample testing basically takes into account all the issues that can be considered. It is currently the most complete technical solution for mixed sample testing. Regarding the reason why the "one-step method" cannot be used, I think it is probably because the one-step method is common. The sample volume is relatively small and direct lysis is used. The purity of the nucleic acid is not high, and the presence of protein and polysaccharides will affect the results.   The purpose of mixed sample detection is to improve the detection efficiency and reduce the detection cost. If the cost factor can be put later, there is also a mixed sample detection scheme that is also good, that is, nucleic acid extraction through a single sample and magnetic bead transfer mixing method Concentrate. This solution uses multiple extraction reagents + 1 detection reagent combination, which can reduce the cost of detection reagents, but the cost of extraction reagents does not decrease much.   The inactivated Virus Transport Media developed by Desheng provides a strong guarantee for nucleic acid detection. The company has also specially tested whether the samples stored in the company’s Virus Transport Media are more suitable for one-step detection or magnetic bead detection. In short, two kinds of detection Each method has its own advantages. If you need more professional and detailed knowledge about the product, you can call our customer service or direct online consultation on the official website, and Desun will have professional technology to answer you.

In-vitro diagnostic reagents are a subdivision of the in-vitro diagnostic industry. They are part of medical devices and play an important role in disease prevention, diagnosis, treatment monitoring, and health status evaluation. There are many classification methods for in vitro diagnostic reagents, and there are different types according to different classification principles. The following Desheng introduces you the classification methods and classification principles of in vitro diagnostic reagents.     The most common classification principle is according to the "In Vitro Diagnostic Reagent Registration Management Measures", according to the order of the degree of product risk from high to low. Mainly divided into the third category, the second category, the first category, and implement classified registration management.   According to the management principle, it is also an important classification method for in vitro diagnostic reagents. First, according to the principle of in vitro diagnostic reagents for drug acceptance and review, in vitro diagnostic reagents can be divided into seven categories, including blood type, tissue matching reagents; microbial antigens, Antibody and nucleic acid detection reagents; tumor marker reagents; immunohistochemistry and human tissue cell reagents; human gene detection reagents; biochips; allergy diagnosis reagents. Secondly, according to the in vitro diagnostic reagents accepted and reviewed by medical devices, it includes a total of nine types of clinical hematology and humoral test reagents, clinical chemistry test reagents, clinical immunological test reagents and microbiological test reagents.   The management classification method needs to be specially pointed out that the in vitro diagnostic reagents managed in accordance with the medical device production supervision and management methods, excluding the in vitro diagnostic reagent products that are legally used for blood source screening and radionuclide labeling by the state.   Another is to classify in vitro diagnostic reagents according to the detection principle, which is the current mainstream classification method. According to this classification principle, in vitro diagnostic reagents can be divided into biochemical diagnostic reagents, immunological diagnostic reagents, molecular diagnostic reagents, microbial diagnostic reagents, urine diagnostic reagents, blood coagulation diagnostic reagents, hematology and flow cytometry diagnostic reagents.   Biochemical, immunological and molecular diagnostic reagents are the three main types of diagnostic reagents in my country. At present, nucleic acid diagnostics in molecular diagnostics occupy the main market.   Since its establishment in 2005, Desheng has been focusing on the R&D and production of in vitro diagnostic reagent raw materials. The raw materials of diagnostic reagents include: biological buffers, chromogen substrates, and current chromogen substrates (new Trinder's reagents) include TOOS, TOPS, ADOS, ADPS, ALPS, DAOS, HDAOS, MADB, MAOS, TODB, etc. Buffers include Tris, Bicine, Caps, Mops, Taps, EPPS, etc.

When it comes to biochemical experiments, especially in the field of protein separation and purification, the role of biological buffers cannot be underestimated. A high-quality buffer solution can exhibit strong capabilities in the presence of trace amounts of acid or alkali, as well as the addition of water, greatly suppressing pH fluctuations and creating a stable chemical environment for experiments. This ability is mainly attributed to its unique composition, such as a mixed solution of weak acids and their salts (such as acetic acid HOAc and sodium acetate NaOAc) or a mixed solution of weak bases and their salts (such as ammonia NH3 · H2O and ammonium chloride NH4Cl), which together form what we call a buffer.   The role of buffer is crucial in the separation and purification process of proteins. The purification of proteins is a complex and delicate task, as each protein has its unique properties and requires specific purification methods. In this process, the stability of proteins often depends on the multi-component buffer system used. A good buffer system can not only maintain the solubility of proteins during the experimental process, but more importantly, it can provide the most suitable environment for proteins without damaging their biological activity.   We know that the purification process of most proteins is carried out in vitro, which means they are detached from their original physiological environment. Therefore, the buffer systems corresponding to recombinant proteins in the market are particularly important. These buffer systems can simulate the environment of natural proteins in the body, providing the possibility for stable storage and transportation of proteins.   When choosing and using buffer solutions, we must consider several key factors. Firstly, the composition of the buffer solution. Ideally, the components of the buffer should not interact with proteins in any form as this may affect their function. For example, some enzymes may be inhibited by phosphate groups in phosphate buffer, leading to their loss of function. Therefore, when choosing a buffer, we should try to choose components that are well compatible with proteins and refer to the recommendations in relevant manuals.   In addition, the pH value of the buffer solution is also an important consideration factor. The choice of pH value depends on the actual application of the protein. If proteins are used for biological assays such as enzyme assays, we should choose the pH values that enable enzymes to exhibit optimal activity. When preparing proteins for purification technology, we need to choose an appropriate pH value based on experimental conditions to ensure maximum purification efficiency. Of course, in situations where a specific pH value is not required, we should choose pH values that can enable proteins to exhibit optimal stability.   In this field, Desheng Company has provided us with a wealth of buffer products with its professional technology and rich experience. They specialize in the research and development, production, and sales of blood collection additives, in vitro diagnostic reagents, biological buffers, and luminescent matrices. They can provide us with various buffer materials (such as TRIS, HEPES, MOPS, etc.), and can configure buffer solutions of different concentrations according to our specific needs. This customized service undoubtedly provides us with more convenience and choices for our experiments.   In summary, buffer plays an irreplaceable role in biochemical experiments, especially in protein separation and purification processes. By selecting the appropriate buffer, we can provide the protein with the most suitable environment, ensuring its stability and activity during the experimental process.

Luminol is a chemiluminescent reagent. Among the many chemiluminescent reagents, luminol reagents have high luminescence quantum yield and good water solubility, and can chemically react with various oxidants and have become The most widely used chemiluminescent reagent. Its luminescence mechanism is that of oxidative reaction. They are catalyzed by horseradish peroxidase under alkaline conditions and oxidized by hydrogen peroxide to produce their excited intermediates that return to aminophthalic acid. When they return to the ground state, they emit Photon. Therefore, Luminol reagents have good application value and broad market demand prospects. The advantages and disadvantages of several Luminol synthesis methods are briefly described here.   The current method of preparing luminol mainly has the following synthetic routes:   (1) Using 3-nitrophthalic acid as a raw material, it undergoes a cyclization reaction with hydrazine hydrate, and is reduced with an insurance powder to obtain luminol (J. Chem. Educ., 1934, II: 142～145). This synthetic method has a simple process route, but the disadvantages are: 1. The reaction temperature in the first step is high, requiring 225 degrees; 2. The purification is difficult. The first step requires the use of high-boiling substance triethylene glycol as a solvent, which is difficult to remove. The reducing agent safety powder used in the second step will decompose during the reaction to produce several inorganic impurities, which are difficult to remove; 3. The yield is low, only about 30%.   (2) Using 3-nitrophthalic acid as a raw material, it undergoes a cyclization reaction with hydrazine sulfate, and is reduced with an insurance powder to obtain luminol (Org. Synth. 1949, 29, 78 and Org. Synth. 1949, 29, 8). The synthesis method has made some improvements on route one, but the disadvantages are: 1. The highly toxic hydrazine sulfate is used; 2. The reaction temperature in the first step is 170 degrees, the temperature is too high, and the equipment requirements are high; 3. The reaction produces a lot of The waste liquid and the reducing agent safety powder used in the second step will decompose during the reaction to produce several inorganic impurities, which are difficult to remove.   (3) Monophthalic anhydride is used as the raw material to nitrate with mixed acid to obtain 3-nitrophthalic acid, dehydrate with acetic anhydride to obtain 3-nitrophthalic anhydride, then hydrazine decompose, and finally reduce iron powder Get Lumino. The shortcomings of this synthesis method are: 1. Long synthetic route; 2. Mixed acid nitrification, which generates a large amount of acidic waste liquid; 3. Iron powder reduction, a large amount of iron slag waste, and greater environmental pollution.   Another method of synthesizing luminol or isoluminol using the one-pot method has obvious advantages and beneficial effects compared with the prior art.   1) The method realizes the completion of the three-step reaction in the same pot, without any purification treatment of the intermediate product, and finally obtains the product directly.   2) The method has simple synthesis route, mild reaction conditions, simple operation, and the required reagents are all conventional reagents, and the required equipment is conventional equipment, so the price is low, therefore, the cost required for synthesis is low, suitable for industrial large-scale production .   3) The yield and purity of luminol and isoluminol synthesized by this method are high. The yield of luminol and isoluminol are both above 80%, and the purity of HPLC is above 98%, which can fully meet the industrialization of products. Production and market demand.

Since the discovery of heparin, it has been widely used to prevent and treat various thromboembolic diseases because of its rapid onset, definite curative effect, and anticoagulant effect can be reversed. However, there are many types of heparin drugs with similar names, such as heparin, low-molecular-weight heparin, enoxaparin, natraheparin, etc., which can easily lead to confusion. What exactly are heparin drugs, what types are they, and how are heparins different?  How is heparin extracted? In 1916, Jay Mclean of John Hopkins University in the United States first discovered a substance with anticoagulant effect from animal liver, so the substance was named "heparin". Later, heparin was found in many organs of mammals. At present, most of the medicinal heparin is extracted from the intestinal mucosa of pigs and lungs of pigs and cattle. What are the types of heparin? Heparin is mainly divided into ordinary heparin (UFH), low molecular weight heparin (LMWH), heparin derivatives (such as fondaparinux), heparin analogs (such as danaparin). What does unfractionated heparin mean? Unfractionated heparin is a mixture of sulfated glycosaminoglycans (GAGs). It is a mucopolysaccharide sulfate composed of alternating D-glucosamine, L-iduronic acid, and D-glucuronic acid. Or made from the intestinal mucosa of cattle, sheep and pigs. What are the low molecular weight heparins? Low molecular weight heparin is a short-chain preparation isolated from ordinary heparin or degraded by ordinary heparin. Due to differences in molecular size, anticoagulant activity, preparation methods, manufacturers, etc., clinically used low molecular weight heparins include enoxaparin, dalteparin, natraparin, etc. What are heparin analogues? Heparin analogue actually refers to a substance similar to heparin, which is somewhat similar in chemical structure to heparin, an acidic substance with anticoagulant activity, heparin analogue danaparin sodium is a mixture of sulfated aminodextran , Also prepared from pig intestinal mucosa, the main components are heparan sulfate, dermatan sulfate and chondroitin sulfate. Indana heparin sodium is rarely used clinically.

Carbomer is a white powdery substance, easy to absorb moisture and agglomerate, soluble in water, ethanol, alcohol and glycerin. Its 1% aqueous solution has a pH of 3.0 and can be neutralized with alkali. The hydrogel formed by carbomer is the most viscous when the pH is 6-12. When the pH is 12, the viscosity decreases. The presence of strong electrolytes will also reduce the viscosity. When exposed to sunlight, it will quickly lose its viscosity. The addition of antioxidants can slow down the reaction.   Many manufacturers will encounter various problems when using carbomer, because carbomer is extremely hydrophilic, and the dry powder of carbomer (carbomer) is very hygroscopic, just like other hygroscopic powders. When improperly put it into water or other polar solvents, it is easy to form agglomeration or incomplete wetting. Other powders will eventually reduce and dissolve after agglomeration, but carbomer will not dissolve easily after agglomeration, because once the outer layer of the agglomerate is completely infiltrated, moisture will not easily penetrate into the inner dry part, and finally appears Massive phenomenon.   Carbomer dissolved in water   A few days ago, several customers asked us how to avoid the phenomenon of carbomer formation. Here are several methods for reference.   1. Sprinkle carbomer on the water (note: it is carbomer on the water, not carbomer with water), let it stand for one night to fully dissolve;   2. Grind the carbomer and glycerin (or propylene glycol, depending on the prescription) in the mortar evenly, then add water to grind evenly;   3. Add a certain amount of water to the agitator, slowly add carbomer under rapid agitation, and continue to agitate for 1-2 hours after the addition is completed, so that it fully dissolves and swells;   Here are some additional points:   1. If it is a small test in the laboratory, it is recommended to use the method 2 or 3;   2. If it is a pilot test or production, the methods 1 and 3 are more suitable. The method 1 may have jelly-like clumps, but the problem is not big: after the colloid mill, you can get a very uniform colloid. After the colloid mill, there may be more air bubbles, which can be defoamed by vacuuming and placing it overnight.   3. The above method only obtains carbomer colloid. To obtain the gel matrix, the pH must be adjusted to 6-10 with triethanolamine or sodium hydroxide solution. The resin particles must be evenly dispersed in cold water. Carbomer can be sieved into agitated vortex with high-speed stirring at 500-800rpm. Optional dispersing equipment can be ejector, flocculation disperser, and conventional disperser. A   The above method is purely personal experience. Each company uses different carbomer manufacturing equipment, and the method also varies from person to person. If you have a better way to avoid carbomer formation, please leave a message for advice, carbomer has For many different models, Desheng currently sells the Carbomer 980 and Carbomer 940 relatively well. After the equipment has been upgraded, it has reached a very good mass production.

As the world's second largest in vitro diagnostic (IVD) market, my country's IVD industry has the characteristics of large development space and high growth rate. Among them, chemiluminescence occupies nearly 40% of the entire IVD market and is a well-deserved "flow king". Why can chemiluminescence explode in the IVD field so that it can occupy a place? First of all, chemiluminescence has the advantages of high degree of automation, safety and stability, high accuracy, and wide detection range compared with traditional immune technology, and has become the mainstream technology of immunodiagnosis in my country. Chemiluminescence is a diagnostic method that uses specific reactions between antigens and antibodies to determine the concentration of disease markers in the body to judge the state of the human body, including enzymatic chemiluminescence (Luminol and its derivatives, AMPPD), direct chemiluminescence ( Isoluminol, acridinium ester), electrochemiluminescence (terpyridine ruthenium), etc. Chemiluminescence is currently widely used in tumors, infectious diseases, nail function, kidney function, heart disease, endocrine hormones, pregnancy testing and other directions, which can greatly meet the needs of clinical testing. These test items account for 75-80% of the total test amount and 60% of the market value; in China, these test items can account for more than 80% of the market value. Secondly, the chemiluminescence technology did not sink fully to the primary hospitals, so there is a large space for development. Although with the development of chemiluminescence technology, its detectable items have become more and more abundant, but at present, the chemiluminescence technology has not completely sunk to the primary hospital. At present, China's chemiluminescence instruments are mainly concentrated in tertiary and secondary hospitals, and some primary hospitals, communities, townships and other grass-roots hospitals have not been installed. With the advancement of graded diagnosis and treatment, the number of outpatient clinics continues to lower the level of these hospitals. There is a wide demand for chemiluminescence instruments and reagents, that is to say, there is still huge room for development in domestic chemiluminescence. In summary, the reason why chemiluminescence is becoming more and more popular in the in vitro diagnostic industry is mainly due to two reasons. First, chemiluminescence has a large market in China because of its special advantages. Second, in the future, chemiluminescence will further sink to the grassroots, covering the needs of hospitals at all levels, and there is great room for development. Since 2005, Desheng has been researching and producing various raw materials for blood collection tube additives, biological buffers, chemiluminescent reagents, etc. It is hoped that with the joint efforts of Desheng people, it will gain its own world in the in vitro diagnostic industry.

Viruses, the most primitive and smallest life on earth, may have existed for 4 billion years. We need to parasitize inside cells. We don’t know whether there are cells or viruses first. At this moment, As a virus, I fell into a tube of Virus Transport Media, and looking back at history can be described as a tumultuous year. Virus infected cells   The war between viruses and cells may have lasted 1 billion years or 4 billion years. No one knows, but it must be the longest jihad on this planet. This jihad also caused us to "jump out of the three realms, Viruses that are not in the "Five Elements" are constantly evolving. No, the new coronavirus is our challenge to the human being, the soul of all creatures called the highest creature on earth. Many humans are hindsight, but in fact the battle of viruses has already started, and listen to me: Virus intrusion For us who have evolved for 4 billion years, it is not difficult to invade the human body. Even if the skin can resist most viruses, fortunately, the mouth, nose, and eyes are open channels. Maybe just a sneeze, we can infect the surroundings. Humanity. But our intention is not to kill humans, but to reproduce, so from SARS to the new crown, we continue to evolve towards low toxicity. Of course, there are also not enough "smart", such as Ebola virus and MERS is a high lethal rate. Virus attack cell Our viruses need to be parasitic, so invading the human body requires advanced attack cells. First, we must avoid the Y-type protein-antibodies patrol back and forth between cells. Once identified, they will be locked by antibodies and then swallowed by white blood cells. Some of our viruses can reach the cell membrane on the cell surface after breaking through the defense line. There are also hundreds of receptor proteins. Large molecules must have special protein keys if they want to enter. After billions of years of evolution, our prominent fiber tail has already obtained the key, so that the virus army has successfully infiltrated into the cell. Virus hijacks the nucleus After the virus enters the cell, it will be sent to the sorting station, the endosome, which is acidic, which will acid off the virus capsid and then break down the virus. This looks like the end of the virus, but when the virus fiber is broken down, the special protein released will tear the endoplasmic wall membrane, and sacrifice a part of the virus-accompanied virus companion, the virus army can develop like a cell nucleus Too. First of all, we combined the motor protein under the cell membrane and used the energy of the mitochondria, a power station that swims inside the cell, to reach the surface of the nuclear membrane. There are many completely different channels here, and billions of chemical signals and instructions are transmitted between DNA and cells through these nuclear pores. We have a forged pass on the viral capsid that locks the tentacles of nuclear membrane proteins, but because it is too large to enter directly, the reverse movement of the motor protein tears the virus. It seems to be a disaster, but it allows us to expose the nucleic acid of the virus through the nuclear hole and enter the cell's headquarters-the cell nucleus. So far, we have successfully hijacked the cell, and then control the nuclear replication virus, let it destroy itself. But now, I am trapped in a tube of Virus Transport Media, the cells will counterattack, and humans will also counterattack. Various new vaccines are also intensifying research and development. This virus holy war has not ended, and will continue...

The appearance of carbopol 940 is white loose powder with characteristic slight odor and strong hygroscopicity. Also known as Carbo 940, it is an acrylic cross-linked resin obtained by cross-linking pentaerythritol and acrylic acid. Carbo resin exists in water in an acidic form, and swells easily in water and polar organic solvents (such as ethanol, glycerin, etc.). Carbopol 940 contains polyalkenyl polyether crosslinked acrylic polymers, which contain 56%-68% carboxylic acid groups in the molecule, making these resins weakly acidic, although weaker than acetic acid, it is easy to interact with inorganic bases and organic bases The reaction produces salts. Due to its swelling and weak acidity, it is a very important rheology modifier. The neutralized carbomer resin is an excellent gel matrix, with important properties such as thickening and suspension, due to the simple process and good stability. The advantages are widely used in lotions, creams, gels. Application of carbopol 940 in skin gel: Allantoin gel prepared with 1.5% carbomer 940 is used to treat dry skin, psoriasis and other skin diseases. Clinical results show that the gel has good compatibility with the skin, lasting effect and no greasy rubbing on the skin Feeling bored. The erythromycin gel prepared with 1% carbomer 940 was used to treat acne. The results showed that the number of acne and the diameter of the base were significantly reduced. The gel for ultrasound diagnosis developed with carbopol 940 as the main matrix has the advantages of non-irritating to human skin, not damaging the probe, non-staining clothing, spreading lubrication, suitable viscosity and stable preparation. It has been proven by clinical use. The quality index achieves the predetermined effect. In addition, ketoprofen preparations were prepared with 4 different bases, and the drug was found to be released fastest from the carbomer gel, and the release rate was in the order of carbomer gel>hydrophilic ointment>cold cream>white petrolatum , Suggesting that carbomer has a certain role in promoting the transdermal absorption of drugs. Application of carbopol 940 in pharmacy: The application of carbopol 940 in pharmaceuticals is mainly manifested in the application of gels. It is used in various oral gels and dental gels. It can be developed into a compound gel preparation with suitable consistency, no greasy feel, and easy to apply. , The preparation is used for the treatment of periodontitis, gingivitis, oral mucosal ulcers, the effect is relatively fast, the effect can be maintained for a long time. Carbomer 940 gel matrix has good film forming and adhesion. Adding protein coagulant containing formaldehyde, thymol and other desensitizing drugs to the gel matrix can prolong the residence time of the drug in the teeth. Desensitization effect is enhanced. Desheng is located in the United Technology City, Gedian Development Zone, Ezhou City, Hubei Province. It is specialized in the research, development, production and sales of blood collection tube additives, biological buffers and luminescent substrates. Provide products and raw materials solutions for more than 100 domestic and foreign manufacturers. The Carbomer 940 developed and produced has good looseness, high transparency and no impurities. If you have product requirements or knowledge, please email to consult.

Recently, I received a complaint from a cooperating customer that the main reason is the bitter experience of purchasing carbomers before and his personal thinking. I feel that it is worth learning from peers. The original text is as follows: Not long ago, I received an arrangement from the company to let us purchase carbomer. The amount used was not very large and was fixedly supplied by foreign manufacturers. However, due to the epidemic situation, our raw materials were cut off. I am not very familiar with this raw material, so I repurchased it. There were many crashing problems in the process. During the procurement period, I asked many domestic carbomer suppliers, some were manufacturers, and some were trading companies. The first problem I encountered was that Carbomer's CAS number often didn't match up and Carbomer's different models were sometimes very confusing. Asking their sales staff, many of them also said it was confusing and ambiguous, which was really a headache. The following is my personal experience and insights, just for reference. Carbomer CAS number: 9003-01-4 139637-85-7 9007-17-4 9007-20-9 9062-04-8 54182-57-9 76050-42-5 The above are collected carbomer cas numbers, including but not limited to these. Although chemical substances and cas numbers correspond in principle, carbomers are acrylic polymers. Different polymerization degrees and different raw materials added in the polymerization reaction will make the reaction products different, and the accuracy of online information is not high. It leads to a lot of cas numbers and chaos, this need not be too tangled.   Undissolved Carbomer powder   Therefore, it is usually inconvenient to express the cas number as a polymer, and it is more common to distinguish by model. Such as Carbomer 940, Carbomer 980, Carbomer 941, Carbomer u20, Carbomer 340 and so on. So the more crazy question is, what does this model mean? There are two main types of network transmission: Does the Carbomer model indicate a different viscosity of Carbomer? This is obviously wrong. If the same carbomer is configured into gels of different concentrations, the difference in viscosity will be very large. The carbomer model is obviously not the number after the gel is configured. This can obviously be directly denied, the same The carbomer model represents the concentration used in different products is also wrong. Does the Carbomer model indicate its degree of polymerization? In a certain encyclopedia and a certain encyclopedia, it is said that the carbomer model represents a different degree of polymerization, the larger the number, the higher the degree of polymerization. At first glance, this statement seems to have a bit of truth, but this statement is also problematic, such as Carbomer 940 and Carbomer 941, the degree of polymerization is only 1 monomer apart, obviously knowing that industrial production is wrong, When the polymerization occurs, the polymer can hardly control the degree of polymerization at 940 or 941. To sum up, personally think that the number of carbomer number should be disassembled and understood. Carbomer is a resin, which may be similar to ion exchange resin. The model of the ion exchange resin is composed of three Arabic numerals, the first digit represents the classification of the product (codes from 0 to 6: strong acid weak acid strong alkali weak alkaline chelating amphoteric redox), the second digit Represents the difference of the skeleton (styrene acrylic acid acetate epoxy system, etc.), the third digit is the sequence number to distinguish the difference of genes, cross-linking agents, etc. Carbomer's model number may represent rheology, light transmittance, monomer difference, cross-linking agent difference, etc., viscosity and degree of polymerization are also included, but it is represented by a number code. Since I asked many companies and many websites, I didn’t find the exact difference between different carbomer models, so I directly asked many carbomer samples to come back for testing. Finally, I chose the carbomer from Desheng for our no-wash The effect in the disinfection gel is very good. In the end, the problem of the meaning of the carbomer model representative has not been completely resolved, and experienced seniors are welcome to give pointers!

Recently, the rebound of Beijing's new crown epidemic has sounded the alarm for our life in the post-epidemic period. The epidemic situation in the United States, Brazil, India, Africa and other regions is also intensifying. The new crown virus will inevitably leave a striking stroke in human history. In order to gain a deeper understanding of this RNA virus, we conducted a brief interview with the company's technology research and development department.   Hubei's new Desheng is a technology company specializing in the production of blood testing related reagents in the Optical Valley United Technology City. At the early stage of the outbreak, it began to invest heavily in the research and development of RNA virus Transport Medias, and eventually produced products approved by many companies.   So we made an appointment and interviewed the engineer Liu responsible for technology research and development. A series of interview questions are as follows:   Viruses and nucleic acids   Q: The company originally made blood test reagents. Why did it decide to make virus Transport Media? A: The company did start as a blood collection tube reagent, but that is only one of the main series in our products. The company has always produced a series of biochemical detection related reagents such as blood collection tube reagents, biological buffers, and sample tube Transport Medias, and even There are also some emerging reagent materials. Our advantage is R&D synthesis and innovation. And the virus Transport Media is an important part of the virus detection process. The market is in urgent need, and we are doing our best for the society. Q: The news now says that the nucleic acid test has false negatives. Is this related to the virus Transport Media? A: There are many cases of false negatives. Instead, the virus Transport Media is to reduce the occurrence of false negatives and increase the accuracy of detection. Because the virus will be quickly lysed in vitro, it is usually not detected in time after sampling. It can keep the original characteristics of the samples during transportation and storage to ensure the accuracy of the detection. Of course, if the virus Transport Media deteriorates or grows bacteria, it will not be able to protect viral proteins or viral RNA.   Q: How do you tell if the virus Transport Media has deteriorated or grown bacteria? Generally, it can be observed by the naked eye first. Usually, the non-inactivated virus Transport Media is easy to deteriorate or grow bacteria, and the inactivated type is not easy to deteriorate. Deteriorated storage solution is usually uneven in color, accompanied by turbidity or flocs, of course, the normal color is ultimately determined by testing to determine whether it is available.   Through the interview, we learned some common problems of virus Transport Media in the sampling tube. Finally, Engineer Liu also shared some suggestions for avoiding the deterioration of the Transport Media. The main reason is that the temperature is too high during transportation and the air during the packaging process. The contact is contaminated with bacteria and fungi, so be sure to keep strict low temperature and the product container is sealed tightly, especially the non-inactivated Transport Media.

The speed of medical tests is of great clinical significance. Some tests require the separation of serum from blood concentration in order to perform. It usually takes one hour from blood clotting to hemorrhage extraction. Even if heated centrifugation is used, it takes half an hour, and it is easy to cause hemolysis. In case of emergency blood distribution or emergency diagnosis, delay time and delay treatment. Therefore, shortening the time of serum separation is an urgent problem to be solved in the current inspection work. In this case, blood coagulant was born.     Blood coagulation:   (1) The concept of promoting coagulation: The process of accelerating blood coagulation by introducing some substances is blood coagulation.   (2) Blood coagulant: substances that can accelerate blood coagulation are called blood coagulants. Blood coagulants include silica powder, glass, fiber, hair, thrombin, snake venom, and rabbit brain powder.   (3) The principle of blood coagulation: blood coagulation is referred to as coagulation, which is the process of changing blood from a flowing state to a gel state. It is an important part of the hemostatic function. The coagulation process is a process in which a series of coagulation factors are activated by successive enzymatic hydrolysis, and finally generates thrombin, forming a fibrin clot. There are 14 factors involved in coagulation. Among them, there are 12 numbered with Roman numerals (from I to VIII, of which factor VI does not exist).   The coagulation process is usually divided into: ① endogenous coagulation pathway; ② exogenous coagulation pathway; ③ common coagulation pathway.   Vacuum blood collection tubes with coagulants sometimes have filaments and lumps precipitated by fibrin, which are caused by the lack of standardized use of coagulant blood collection tubes. In the preparation of vacuum blood collection tubes, the selection of procoagulants with too fast coagulation speed will cause fibrin to contract too fast and the fragile red blood cells to break easily, causing mild hemolysis. There are the following reasons for the precipitation of fibrin: the use of coagulant blood collection tubes or separation gel to promote coagulation blood collection tubes, if the coagulant is evenly distributed in the blood, it must be slightly inverted and mixed 4-5 times, so that the center of the blood collection tube and the surrounding The blood coagulates at the same time. If the blood is not completely coagulated, it is centrifuged, which can cause the precipitation of fibrin. A jelly sample or a lump sample appeared on the upper part of the serum, mixed with blood. Fibrin filaments are caused by fibrin that has not contracted completely. When using coagulant blood collection tubes, the spraying of coagulant is insufficient or the prepared water-soluble coagulant is not used up on the same day, and the continued use the next day leads to a decrease in the effectiveness of the coagulant. Fibrinogen in the blood is gradually transformed into insoluble fibrin under the action of a coagulant. If the dose of the coagulant is insufficient or fails to extend the coagulation time, centrifugation can cause fibrin precipitation.