Application of EDTA-2K anticoagulant in erythrocyte sedimentation rate detection

ESR (erythrocyte sedimentation rate ESR) refers to the sinking speed of red blood cells under certain conditions. ESR can increase significantly in many pathological conditions. The pathological reasons leading to the marked increase in erythrocyte sedimentation rate are mainly found in various inflammations, tissue damage and necrosis, malignant tumors, hyperglobulinemia and anemia. ESR is often used clinically to observe the activity and dynamic changes of tuberculosis and rheumatic fever.

Because most of them need to test the blood routine at the same time, the blood collection volume is relatively large, especially for children. The author uses EDTA-2K+ anticoagulant to determine and evaluate the erythrocyte sedimentation rate, and make related experiments. The experimental method is: take about 3.2 mL of the subject's venous blood, take 1.2 mL and put it into an erythrocyte sedimentation tube containing 0.3 mL of 109 mmol/L sodium citrate anticoagulant, gently invert and mix; then take 2.0 mL of venous blood The human blood test tube containing 30ul 15% EDTA-2K anticoagulant is gently inverted and mixed, and then 1.2 mL of it is added to the erythrocyte sedimentation tube containing 0.3 mL of 109 mmol/L sodium citrate anticoagulant. The tube also measures the erythrocyte sedimentation rate.

Blood collection tube additive EDTA-K2

The principle of anticoagulation between EDTA and sodium citrate is to form a chelate with calcium ions in the blood to prevent blood from clotting. However, the anticoagulant effect of EDTA is stronger than that of sodium citrate, and the two do not interact with each other. For interference, the principle of using the automatic erythrocyte sedimentation rate analyzer is dynamic erythrocyte sedimentation rate analysis. Sodium citrate anticoagulant blood is added to a special test tube, and the fixed hole inside the instrument is vertically placed. According to the setting, the photoelectric detection part of the instrument will scan each cuvette up and down regularly. According to the different light transmittance of glass tube plasma and whole blood, the instrument automatically records the plasma height each time. Through computer processing, the instrument can report the erythrocyte sedimentation rate of 60 minutes and draw the erythrocyte sedimentation time curve.

The experimental results show that the ratio of anticoagulant to whole blood has a great influence on the erythrocyte sedimentation rate, and a high anticoagulant ratio will cause a low erythrocyte sedimentation rate. The test tube used in the instrument method has a small diameter. In actual work, it is common that the amount of blood collected from the sample is not accurate; and the EDTA-2K+ anticoagulant blood measured by a blood cell analyzer is used for the erythrocyte sedimentation rate determination, and the same charge is maintained by complementing the anticoagulation ratio , To ensure the accuracy of the results. This article proves through experiments that EDTA-2K+anticoagulant blood can be used for instrumental ESR determination, and the results are accurate and reliable.

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