Introduction: buffer is a kind of solution that can resist pH change when adding a small amount of acid or alkali and water. PH buffer system plays an important role in maintaining normal pH value and normal physiological environment of organisms. Most cells can only move in a narrow pH range, and buffer systems are needed to resist pH changes during metabolism. There are three main pH buffer systems in organisms, They are protein and bicarbonate buffer systems. The amount of each buffer system is different in various cells and organs
In biochemical research, buffer solution is often used to maintain the pH value of experimental system. The change of pH value of solution system in research work often directly affects the effect of our work. If the pH value of extraction enzyme experimental system changes or changes too much, the enzyme activity will decrease or even completely inactivate. Therefore, we should learn to prepare buffer solution
Bicine is an amphoteric amino acid buffer. It is active in the range of pH 7.6-9.0 (PKA = 8.3 at 25 ° C). Recommended buffer for low temperature biochemical work. Bicaine was used to prepare stable serum guanosine substrate solution. A method of protein separation using dihydropyridine in thin layer ion exchange chromatography has been reported. Dihydropyridine has been used for peptide and protein crystallization.
Standard for finished buffer
Preparation method of bicine solution (about 1-2l)
(1) 0.1M solution (a): bicine 16.317g/deionized water 1000ml
(2) 0.1M NaOH solution (b): 4G NaOH / 1000ml deionized water
(3)pH 5.1 1,000ml(A)+0ml(B) (A):(B)=5:0
pH 7.8 1,000ml(A)+200ml(B) (A):(B)=5:1
pH 8.2 1,000ml(A)+400ml(B) (A):(B)=5:2
pH 8.6 1,000ml(A)+600ml(B) (A):(B)=5:3
pH 10.4 1,000ml(A)+800ml(B) (A):(B)=5:4
* temperature 20 degrees.
* if you need to adjust to a specific pH, use a pH meter.
* if you don't want to join Na, please use KOH.
Hubei xindesheng Material Technology Co., Ltd. specializes in the production of various biological buffers, including Tris, Tris HCl, bicine, caps, mops, taps, EPPs, MOPSO, HEPES, pops, if necessary, please contact us for details.
Contact Person: Miss. Ankiwang