1. As an inducer and self-oxidant

Luminol is a light inducer and self-oxidant. It can be used as a free radical detection reagent as well as a photosensitizer. In PCL analysis, auto-oxidation is inhibited by a single or a group of antioxidant compounds in the nanomolar range. The antioxidant potential of the sample can be measured by studying the lag phase at different concentrations.

2. Detection of blood luminescence reagent

The blood absorbs ultraviolet rays and turns black. After chemical treatment, the blood will also fluoresce. Luminol is commonly used to produce this chemiluminescence, which is a reagent that reacts with catalase in hemoglobin in the blood.

3. Time interval used to identify skeletal remains

As the femoral PMI increases, chemiluminescence decreases. In femurs with a PMI of 1 month to 3 years, strong chemiluminescence is observed after a few seconds. Patients with a PMI of 10-15 years have clear chemiluminescence, and 80% of the samples are visible to the naked eye; femurs with a PMI of 25-35 years have weak chemiluminescence in 33% of the samples. Femurs with a PMI of 50-60 years have only a weak response observed in a single femur. No chemiluminescence was observed in femurs whose PMI was over 80 years old.

4. Detect the influence of serum prostate specific antigen

The prostate-specific antigen in serum can be detected by luminol chemiluminescence enzyme immunoassay, which has high sensitivity, simple and quick operation, and strong specificity. When tPSA is greater than 10ng/ml, prostate cancer is highly suspected. When tPSA is between 4-10ng/ml, it is called gray value, and the diagnosis of prostate cancer should be combined with the ratio of fPSA/tPSA.

5. Bioluminescence imaging for inflammation

Luminol can achieve highly sensitive bioluminescence imaging of inflammation through the luminescence reaction with myeloperoxidase MPO produced in the inflammatory area, which is important for the diagnosis of neurodegenerative diseases, atherosclerosis, cancer and other diseases significance.