Desheng is a material technology company engaged in R & D, production and sales. We will make a series of comparisons for each series of products, so as to help customers better distinguish the differences between them and better find the products they need. Let's briefly describe the differences and similarities between good's buffer pipe and HEPES.
The pH buffer range of pipes is 6.1-7.5, insoluble in water and soluble in NaOH solution. Different from buffers containing bis (2-hydroxyethyl) amino groups (such as bis Tris, bicine), pipes can not form stable complexes with most metal ions, so it is suitable for buffers containing metal ions. According to previous studies, PIPES can be used to purify tubulin by using cellulose phosphate chromatography, and to purify recombinant GTP binding protein ARF1 and ARF2 by gel filtration as a buffer to crystallize ketoenzyme from E. coli. In addition, due to the formation of free radicals, pipe is not suitable for redox system. In cation exchange chromatography, low concentration of pipes buffer should be used because of its relatively high ionic strength and concentration dependent pKa value.
The pH buffer range of HEPES is 6.8-8.2, which is soluble in water and does not form stable complexes with metal ions. In most cases, it does not interfere with biochemical processes. HEPES is commonly used in cell culture media of various types of organisms; in protein research, it is commonly used as component and eluent of binding buffer in cation exchange chromatography; in DNA research, it is used as calcium phosphate and DN A, the buffer solution of precipitate formation system, AFM and electroporation experiment. In addition, HEPES interferes with the reaction between DNA and restriction enzyme and is not suitable for Lowry's method.
Desheng good's buffer
In conclusion, both pipes and HepS belong to good's buffer, and they can not form stable complexes with metal ions, so they are suitable for solution containing metal ions. However, there are some differences between them. In terms of solubility, pipe is insoluble in water, while HEPES has good water solubility. In terms of buffer range, pipe is acidic to neutral, and HEPES is neutral to alkaline. This is mainly due to the structural differences between them. Pipe has two sulfonic groups, and HEPES contains one sulfonic group and hydroxyl group. In addition, pipes and HEPES have some limitations in the application of some systems. Therefore, when we choose the above buffers, we need to consider the suitability of the experimental system and the difference of their properties.
When you purchase good's products, it's not the buffer you need. Desheng will also give you an accurate positioning.
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