Biological buffers are an important part of electrophoresis technology and are generally added to the solution to stabilize the pH when current is passed through the sample. In addition, the buffer can also provide the ions needed for electrophoretic migration.
The ideal buffer for electrophoresis depends on the isoelectric point of the sample being analyzed.
Although there are many pre-made buffers for electrophoresis on the market, it is recommended to use the biological buffers prepared by yourself to make your own solutions.
Our company is a professional manufacturer of biological buffers. For this reason, we have created a list of biological buffers frequently used in electrophoresis, so that it is convenient to see which biological buffer is more suitable for you.
1. Tris buffer
Used for: gel electrophoresis
Useful pH range: 7.5-9.0
pKa (25°C): 7.8-8.2
Molecular weight: 121.14
2. MOPS buffer
Used for: gel electrophoresis
Useful pH range: 6.5-7.9
pKa (25°C): 7.0-7.4
Molecular weight: 209.3
3. Bicine buffer
Used for: gel electrophoresis
Useful pH range: 7.6-9.0
pKa (25°C): 8.1-8.5
Molecular weight: 163.2
4. CAPS buffer
Used for: Capillary Electrophoresis
Useful pH range: 9.7-11.1
pKa (25°C): 10.2-10.6
Molecular weight: 221.32
5. CAPSO buffer
Used for: gel electrophoresis
Useful pH range: 8.9-10.3
pKa (25°C): 9.4-9.8
Molecular weight: 237.32
It can be seen from the above that there are many biological buffers used in electrophoresis, and there are some differences between them, and they can be used in different electrophoresis. In the current market, there are many vendors of biological buffers, and product specifications and quality are uneven. Our company is a manufacturer specializing in the production of biological buffers. It has been established for more than ten years and has very rich R&D and production experience and product knowledge. It can provide customers with a large amount of technical support and after-sales guarantee.
Hubei Xindesheng Materials Co., Ltd. can also provide customized packaging and other services according to customer needs, and our products can be delivered to most areas at home and abroad. In addition, our company also produces blood collection tube additives, chemiluminescence reagents, chromogen substrates, carbomers and other products. If you are interested in our products, you can enter our official website to contact customer service for details.
