China Wuhan Desheng Biochemical Technology Co., Ltd Company News

The full name of Tris base is tris(hydroxymethyl)aminomethane. It is a widely used biological buffer. Its hydrochloride solution is not only widely used as a solvent for nucleic acids and proteins, such as TAE and TBE buffers, especially nucleic acid solutions. . It contains primary amines, so reactions related to typical amines occur, such as aldehydes. Tris also complexes with metal ions in the solution.   As a domestic tris manufacturer, Desheng Biotech produces high-purity white crystals in powdered tris. Recently, I received a call from a customer and reported the high temperature situation. The viscosity of the product increased significantly around 126 degrees. The originally dispersed powder gathered into fine particles, and the reaction was carried out in an anhydrous dry reactor, basically eliminating the influence of moisture. Will this change affect product quality?   TRIS base powder   In fact, this involves the effect of temperature on the crystal structure of Tris. The molecular crystal structure responds to changes in externally applied conditions (such as temperature and pressure) in a complex balance between strong intramolecular bonding, moderate intermolecular interactions (such as hydrogen bonds) and weaker intermolecular van der Waals contacts result. Usually we also consider the influence of pressure on the crystal morphology. We have passed the autoclave test. This pressure above 75Mpa obviously affects the crystal shape. The additional thermodynamic requirements for effective space filling become more and more important. However, the pressure of the autoclave usually does not exceed 20Mpa, so the influence of pressure is not discussed here.   Our measurements are all performed using neutron powder diffraction. The characteristic of the crystal structure is the van der Waals interaction between the molecular chain formed by the NH...NH bond. Although hydrogen bonding is the strongest intermolecular contact, most of the sublimation enthalpy may be due to weaker but numerous van der Waals interactions. Analysis of the thermal expansion data based on the phonon frequency determined in the periodic DFT calculation shows that the expansion at very low temperatures is controlled by the external lattice mode, but above 126°C, the influence of the deflection mode of the intramolecular ring also changes Significantly. The main direction of thermal expansion depends on the sensitivity of different van der Waals interactions to distance changes.   The main value of strain generated when pressure is applied is similar to the value determined in the variable temperature study, but it is isotropic because it needs to fill the void under high pressure to minimize the volume. When the temperature is lowered to room temperature, the hydrogen bond between the molecules weakens, the H interaction between the molecules, the interaction between the dipole moments makes the force between the molecules weaker, and the tris presents a loose white powder structure. This is what we are. The shape of the finished product usually seen. The above changes are all physical changes, which are instantaneous changes in the polarity between molecules and do not change the chemical properties of the molecules themselves, so there is no product quality problem. You can use it with confidence.   Desheng Biochemical is a high-tech enterprise focusing on the fields of life science and biotechnology, providing Chinese experts and scholars with a full range of high-quality products. The tris, Tris-HCL, Bicine, CAPS, MOPS, TAPS,HEPES, etc. produced have been widely used in life science basic research, development and application, biotechnology and many other fields. Customers are located in universities, research institutes, hospitals, biotechnology companies and food industry units.

Blood anticoagulant is the application of physical or chemical methods to remove or inhibit some blood coagulation factors, prevent blood coagulation, known as anticoagulation. Chemical agents or substances that can prevent blood coagulation are called anticoagulants or anticoagulants.   Desheng biochemical is specialized in the R & D, production and sales of vascular additives, in vitro diagnostic reagents, buffers and luminescent substrates. In the aspect of blood vessel additive, it has formed independent intellectual property rights and professional production and research capacity.   To provide products and raw material solutions for more than 100 domestic and foreign manufacturers. Desheng provides 6 different blood coagulants for you to choose from. What are the similarities and differences between these 6 blood coagulants? Let's make a comparison.   Heparin sodium   White amorphous powder, odorless, soluble in water, easy to absorb moisture, average molecular weight of 15000, stable at room temperature. The heparin sodium titer of our company is more than 150 IU / Mg, and the anhydrous titer is more than 160 IU / mg. Other indexes were controlled according to the standard of heparin sodium.   This product is suitable for the collection and anticoagulation of blood samples in clinical biochemical examination and emergency biochemical examination, and also suitable for the collection and anticoagulation of blood samples in some Hemorheology projects.   Heparin sodium is recommended as an anticoagulant in the determination of ion content in blood, because it is the least likely to interfere with the determination of other ions. This product is not a drug and can not be used as an injection. It is forbidden to directly inject into human body and animals.   Heparin lithium   It is also a white amorphous powder, which is replaced on the basis of heparin sodium. It is stable at room temperature. The routine potency of heparin lithium was more than 150 IU / Mg, which was controlled according to the standard of heparin API.   When heparin lithium has impurities, abnormal smell, color and beyond the validity period, and heparin lithium solution appears turbid, it may be that heparin lithium solution has been contaminated or deteriorated, so it is forbidden to use.   Potassium oxalate   White monoclinic crystal, soluble in water, used in medicine and straw hat bleaching; also used as chemical reagent, fabric detergent; used as anticoagulant for venous blood vessel samples.   White crystal powder, odorless, soluble in water, easy to absorb moisture, molecular weight 404.6, pH 4.8 ± 1 (5% aqueous solution, g / ml), water solubility ≥ 60% (g / ml, 25 ℃). It is stable at room temperature. The main content of potassium EDTA in our company is more than 99.0%. Other indicators are shown in the product test report.   When there are impurities, foreign matters, abnormal smell, color, serious moisture absorption and beyond the validity period of EDTA, it is forbidden to use.   Potassium EDTA   This product is specially used for in vitro anticoagulation of blood. It is an anticoagulant additive for clinical blood test. It is used for the pretreatment of blood samples (anticoagulant treatment) in the process of clinical blood collection and test.   This product can be sealed in a cool and dry place, away from light and moisture. The water solution of this product can be sealed at normal temperature under sterile condition. It is suggested that this product should be ready to use in water solution, and should not be stored in solution state for a long time.   Sodium citrate   White crystal powder, easy to absorb moisture, can be used as flavor agent and stabilizer in food and beverage industry; used as anticoagulant, expectorant and diuretic in pharmaceutical industry; used as adjuvant of nontoxic detergent instead of sodium tripolyphosphate in detergent industry; used as excellent chelator / complexing agent in chemistry; used in industry for sodium citrate. It is also used in brewing, injection, photographic medicine and electroplating.

Chemical reagent is a kind of fine chemicals with various purity standards, which can be used for teaching, scientific research, analysis and testing, and can be used as pure and ultra pure functional materials and raw materials for some emerging industries. Generally, it can be classified by composition and purity. It is not only an important tool for modern scientific research and product inspection, but also an indispensable material for emerging technology.   According to the major categories, chemical reagents can be divided into 11 categories: General inorganic reagents, general organic reagents, high-purity inorganic reagents, high-purity organic reagents, general analytical reagents, instrumental analytical reagents, biochemical and molecular biological reagents, clinical laboratory reagents, isotope reagents, special chemicals and other reagents. Chemical reagents can also be divided into high-purity reagents, high-grade pure reagents, analytical pure reagents, etc. The specifications of chemical reagents in China are basically classified according to purity. The quality indicators issued by the state and competent departments mainly include superior grade purity, analytical purity, chemical purity and experimental purity.   Chemical reagents of different colors 1. High grade purity (GR, green label) (grade I): high content of main components, high purity, suitable for analysis and research, some can be used as reference materials. 2. Analytical purity (AR, red label) (grade II): high content of main components, high purity, low interference impurities, suitable for industrial analysis and chemical experiments. 3. Chemical purity (CP, blue label) (grade III): high content of main components, high purity, interference impurities, suitable for chemical experiments and synthetic preparation. 4. Experimental purity (LR, yellow label): high content of main components, poor purity, no choice of impurity content, only suitable for general chemical experiments and synthetic preparation.   In addition to the above four levels, there are: Reference Reagent (PT): specially used as reference material, which can be directly prepared into standard solution. Spectral purity reagent (SP): indicates spectral purity. However, due to the fact that the organic matter can not be displayed in the spectrum, sometimes the principal component is less than 99.9%, so we must pay attention to it when using it, especially when it is used as a reference substance. The reagent whose purity is much higher than that of superior grade is called high purity reagent (I > 99.99%). High purity reagents are developed on the basis of general reagents. High purity reagents are especially suitable for trace analysis.   More than 95% of the biochemical reagents produced and developed by Desheng include chromogenic reagents, biological buffers, blood collection additives, chemiluminescence reagents, etc., which are analytical pure reagents with high purity. The company covers an area of 3000 square meters, has modern clean workshop and advanced production equipment, strong technical force, a number of professional R & D teams, and advanced and complete analytical detectors The perfect quality inspection and quality assurance system ensure that we provide stable high-quality products to the market. We warmly welcome all major enterprises to visit our company.

Carbomer is very common in daily chemical products, but people rarely find it. For example, toothpaste, skin care lotion, and bath shampoo all exist in it. It is even more important in the hand-washing disinfectant gel just needed during the epidemic. This kind of hand sanitizer is not liquid and will not flow randomly, and it has disinfection, Features such as no-washing are very suitable for quick disinfection of hands. As an indispensable raw material in daily chemical products, the annual purchase quantity of Carbomer will naturally not be small. So how to choose a high-quality carbomer supplier? In response to this problem, this article analyzes from the following aspects.   Desun Carbomer powder and gel samples   1. Guarantee products and guarantee services   By investigating related companies, we can find that high-quality carbomer manufacturers can master more advanced technologies, conduct in-depth explorations with customers, and negotiate cooperation related matters. In early 2020, the company invested a lot of capital, manpower and material resources, and now has A group of professional technical personnel can guarantee that the indexes of the raw materials produced by Carbomer are qualified and up to standards, comparable to international brands. Not only that, when encountering quality problems, Desheng never shirks, but promptly returns them. To ensure that the interests of customers are not harmed.   2.guarantee the delivery speed   The quality of the purchased product is the key, and the delivery speed is also what many customers care about. If the delivery is too slow, many important customers will be lost. No matter how good the product quality is, once the delivery cycle is long, the customer experience will be greatly reduced. After the equipment is upgraded and modified, the monthly output can reach the ton level, and the delivery is very timely. Under normal circumstances, placing an order on the same day can guarantee timely delivery on the same day. If the quantity is large, it can basically reach the customer in 3-5 days, so here Don’t worry about it.     3.to ensure a very high product-to-performance ratio   When choosing what kind of carbomer raw material company to cooperate with, the quotation provided by the relevant company is also an important reference standard, especially when the demand for carbomer is large, the corresponding raw material purchase volume will also double. If the supplier can provide a more competitive quotation, there will definitely be more room for maneuverability in terms of cost control. Desheng is the manufacturer of Carbomer, direct sales of raw materials. So compared with other traders, the product cost performance will be higher.   I believe that after reading the above analysis, you will understand how people who really know how to choose carbomer raw materials suppliers, I believe Desheng will be your trusted choice. Now the company has begun to implement mass production, with an average daily output of 1t, and has rich experience in exporting. If necessary, please call for detailed inquiry.

When many suppliers refer to carbomer 940, they first think of Lubrizol or Tianci. Because of the monopoly of Lubrizol and its brand cooperation, many customers lose their supply chain. Today, let's take a look at the cabom manufacturers besides Lubrizol and Tianci.   When you are looking for the supplier of carbomer 940, you are used to searching on the search engine. I don't know if you have noticed. Desheng is often ranked in the top. Established in 2005, Desheng is specialized in the production and development of vascular additives, in vitro diagnostic reagents, buffers and luminescent substrates. Cabom is the top priority of the company's products. Desheng employs professional and technical personnel to continuously improve the product quality, and has a special quality department for inspection. Unqualified products will never reach customers.     In order to seize the market popularity of Carbomer, many manufacturers purchase carbomer raw materials at low prices and provide them to purchasers, resulting in a lot of unqualified phenomena after use. Desheng is a professional carbomer manufacturer. Its products are not only of good quality, but also of high cost performance. Now we launch the year-end preferential activities. If the order volume is large, the price is as low as 100 yuan / kg. On the premise of ensuring the quality of products, Desheng also provides perfect after-sales service and promises to return and exchange the goods free of charge if there is any quality problem, which provides the quality assurance for the buyers and solves the troubles for the customers.   Although there are many series of Carbomer, Desheng mainly produces carbomer 940 and carbomer 980, because these two kinds of applications are the most. It should be noted that carbomer has strong moisture absorption, so it must be sealed during storage to avoid moisture absorption and caking in exposed air. Carbomer produced by Desheng is packed in non-toxic polypropylene plastic bag, moisture-proof and airtight, and then packed in cardboard barrel. The conventional packaging is 10kg / bag, and Desheng can pack it according to customers' requirements. Samples can be provided, business friends in need can directly contact the official website customer service to get the use of the equipment.

Since December 2019, the pneumonia epidemic caused by the new coronavirus has spread continuously, affecting the heartstrings of every Chinese. The current epidemic is a great challenge for medical, diagnostic and biopharmaceutical companies from virus detection, biosafety protection of medical staff and testing personnel to detection of patient immune system status. Life is supreme, Desheng fights the "epidemic"-Desheng successfully developed virus preservation solutions at the beginning of the epidemic to help safe and efficient virus detection and clinical testing.   At present, the form of prevention and control of the new coronavirus is still very severe. The Hebei epidemic is worrying the whole country. Many celebrities donate money and materials to support Hebei’s fight against the epidemic. In the face of the epidemic, nucleic acid testing cannot tolerate a sloppy approach. There is no doubt about the importance of collection and preservation. If there is a problem with sample collection, no matter how well the subsequent work is done, the result will be invalid. Choosing the right virus preservation solution can make the new crown detection more effective! Then when choosing a virus preservation solution, we need to pay attention to the following aspects: 1. The manufacturer can ensure the high quality of the virus transport media raw materials Highly responsible virus preservation liquid manufacturers can not only fulfill their promises, but also ensure that the facts are described without exaggeration, such as the strict selection of raw materials to prevent fraud. The irresponsible manufacturers will only exaggerate to promote the order. In this process, the customer should keep his eyes open and distinguish right from wrong.   2. Use inactivated virus transport media The current nucleic acid detection is to detect the viral RNA in the sample, and the virus needs to be lysed, so as long as it can ensure the integrity and stability of the viral nucleic acid, it does not need to save the live virus. Therefore, in the current situation of fighting the new coronavirus epidemic, it is more ideal to inactivate the virus while collecting samples. Therefore, Desheng recommends the use of inactivated virus preservation solution, which can inactivate the virus and ensure that the virus nucleic acid will not be degraded when stored at room temperature.   3. Choose a virus preservation solution manufacturer with safe transportation Because the virus preservation solution is liquid, leakage will occur if the packaging is not in place and a little bump, which will cause very large losses to customers. Therefore, the selection of packaging materials, structure design, manufacturing process, etc. must be It is necessary to check the quality of the product before delivery. Details determine success or failure. No matter how good the quality of the product, without a good purchase experience, many important customers will be lost.   The virus transport media developed and produced by Desheng are divided into inactivated and non-inactivated types. Different types of virus preservation solutions can be selected according to experimental needs. The current situation of the new crown epidemic is severe. People returning from other provinces not only need to be isolated, but also nucleic acid testing. At this time, choosing the right nucleic acid virus transport media can greatly save procurement time and cost. If you have any questions or needs, please call for detailed consultation. Will be your right choice.

The additive is the core raw material in the blood collection. Its quality directly determines whether the clinical test can be carried out in time, the accuracy of the test results and the reliability of the diagnosis results. Desheng, as a manufacturer of vascular additive, has the responsibility and obligation to provide stable quality additive for customers and patients, so as to ensure the accuracy and timeliness of test results.   Analysis of common problems and causes in the use of serum separation gel:     A. Glue difficulty: the temperature is too low. In winter, the temperature drops, the weather is cold, the viscosity of separation gum increases, and the process of adding gum is difficult. On the one hand, in winter, glue is heated. Serum gel can withstand high temperature below 80 ℃. Now many equipment production company glue machine with heating function, for blood collection enterprise provides a solution. In addition, reducing the viscosity of separation gum can also solve some problems, but it is not the fundamental solution.   B. Flow: after the serum separation gel is added, the flow distance of the separation gel is too large when the test tube is placed horizontally, and some even flow to the nozzle. This is because the intermolecular chemical force has not been fully recovered and the network state has not been formed. The first solution is to increase the thixotropy of the separation gel, but it will cause the difficulty of adding the gel; the second is to put it upright for several hours, and then put it flat after the thixotropy of the separation gel is restored.   C. Bubble problem: vacuumize after adding glue, bubbles will appear in serum separation glue, or bubbles will appear after irradiation sterilization. This is because there is invisible air in the separation glue during the process of adding glue. When heated in vacuum or during irradiation sterilization, the air in the serum separation glue slowly expands and becomes visible bubbles. In the process of separating glue production and adding glue, air should be reduced as much as possible.   D. The problem of blood separation glue not turning over: some blood separation glue collection vessels will not turn over in clinical practice, usually due to insufficient centrifugal force or insufficient centrifugal time. Increasing centrifugal force or prolonging centrifugal time will basically solve the problem. There are also some serum gel because of aging and other factors do not flip, belongs to the quality of the gel.     E. Turn the serum separation gel over to the serum: This is caused by the small specific gravity of the separation gel. In recent years, this kind of situation will not appear basically. Before and after 2010, our company had caused this situation because of the detection error, which caused great problems to customers, and the company also lost a lot.   F. Detector alarm: in clinical examination, the problem of blood vessel alarm often appears. In the past, the industry always thought that it was caused by oil drops or fragments of separation glue. After years of follow-up analysis, we have found the root cause of this problem. The alarm of the instrument is that the blood is not completely coagulated and centrifuged, which causes the fibrin filament to float in the serum. When the probe sucks the sample, the fibrin filament is inhaled into the probe, which causes the instrument to block and give an alarm.   Desheng is specialized in the R & D, production and sales of blood collection additives and blood test reagents. In the aspect of blood vessel additive, it has formed independent intellectual property rights and professional production and research capacity. We have accumulated rich knowledge and experience in the pretreatment of blood samples, and have the advantage of professional technical services.

In the field of daily chemicals, there are many kinds of thickeners. Why is carbomer the only one that is in demand? Carbomer is a kind of polyacrylic acid cross-linked polymer, which is mainly used for thickening of daily chemical products, and can achieve the effect at a very low concentration. Such as shampoo, shower gel, detergent, including some cream cosmetics, usually use carbomer to increase viscosity, most daily chemical products contain this component. De Sheng carbomer powder and gel sample   In the past few years, the global market of cabom has developed rapidly, with an average growth rate of 11.98% from 2013 to 2017. In 2017, kapom's global revenue was nearly $736 million; its actual sales volume was about 57600 tons.   In 2020, due to the spread of the epidemic in the world, many domestic and foreign manufacturers of carbomer will be forced to suspend business temporarily due to the epidemic. As a result, the supply of carbomer in the market will be tight, and the price will soar from the initial dozens to hundreds. The crazy index is like a rocket launch. But after going crazy, now capom also slowly tends to calm down, but this calm is also turbulent.   The outbreak of novel coronavirus pneumonia has be taken by surprise for the medical products industry. Masks, protective clothing, disinfection supplies and other medical supplies are seriously in short supply. The shortage of masks has led to drastic fluctuations in the prices of upstream raw materials of masks, such as melt blown non-woven fabrics and polypropylene.   At the same time, there was a shortage of commonly used hand sanitizers during the epidemic period, and the strong demand led to the rapid rise in the price of thickening agent carbomer. But then the market went into chaos, with prices changing every day.   As more and more enterprises join in the industry of producing Carbomer, the price of carbomer has been fluctuating. Although the fluctuation range is not very large, there are many practical problems involved. If you want to buy the product you want at a considerate price, it may take a lot of time and experience. Now I have good news to tell you that it can not only let you buy good products, but also have intimate prices.   In order to thank the new and old customers for their support and love, De Sheng has made great breakthroughs in the transparency of product quality. This month, we have also made a preferential adjustment in the price. For kapom above ton level, we only need to buy it at 100 yuan per kilogram, and it is a spot trip. If we miss this wave, we will have to wait for next year. What are you waiting for? If you have good quality and price, we will take decisive actions. If there are any problems in the later quality, we will solve them for you.

The most commonly used enzyme preparation in enzyme-linked immunoassay is HRP horseradish peroxidase. There are three types of substrates in biochemical detection, and there are mainly three types, namely chromogen substrates such as TOOS, MAOS, and fluorescent substrates. Substrates, chemiluminescent substrates such as luminol. Among them, chemiluminescence substrates are also used in chemiluminescence analysis, which is becoming more and more popular. Whether it is a chromogen substrate, a fluorescent substrate or a chemiluminescence substrate, it is used as a labeling and detection method, which plays a key role in the accuracy and sensitivity of the detection result. The most popular one is chemiluminescence immunity. analysis. luminol powder The development of different substrates in biochemical testing: In terms of the development time of the detection method, the fluorescent substrate is the product of peroxidase developed after the chromogen substrate, and the detection sensitivity is higher than that of the chromogen substrate. Several fluorescent materials of different colors can be used at the same time. , But the equipment is expensive, the detection cost is high, and it is easily interfered by autofluorescence generated by cells or other molecules, and it is sensitive to light and unstable during observation and storage. After fluorescent substrates, the discovery of luminol-like chemiluminescent substrates brought horseradish peroxidase HRP substrates into a rapid development stage, and various chemiluminescent substrates greatly enriched horseradish peroxidase Applications. The difference between chemiluminescence and fluorescence: Chemiluminescence is different from fluorescence. It means that the chemical energy generated in the process of chemical reaction is absorbed to stimulate molecules and emit light. Fluorescence is the light emitted by absorbing light energy to excite molecules. This is chemiluminescence and The difference in fluorescence lies in the different forms of excitation energy that form excited molecules between the two, one is chemical energy and the other is light energy. Chemiluminescence detection has high sensitivity and low background, so it is widely used. Currently common chemiluminescent substrates, including luminols, peroxyoxalates, and acridine esters, are several chemiluminescent reagents commonly used in actual chemical analysis and determination, and are used in drug analysis, environmental monitoring, etc. The field also has important applications. The above-mentioned chemiluminescence reagents are specially produced by Desheng, but it should be noted that acridine esters can emit light directly and do not require enzyme catalysis.

Blood sample detection must be well known by all of us. Basically, many clinical research projects will be involved as indicators to evaluate safety. Some disease-related markers and specific tests will also be carried out through blood samples. Desheng would like to share with you the contents of blood sample collection and preservation.   Blood composition   Blood is mainly composed of plasma and blood cells (so-called blood cells). After the blood is treated with coagulant, it can be found that plasma and blood cells are obviously divided into two parts after centrifugation or standing for a period of time in a centrifuge. Blood cells are deposited at the bottom because of heavy weight, and the yellow transparent liquid suspended above the red blood cells is plasma.   If the blood is naturally coagulated without anticoagulant treatment, the blood will be divided into two parts. The three visible components of red blood cells, white blood cells and platelets are precipitated below; the clear and transparent light yellow liquid floating above is serum, which does not contain blood cells and fibrinogen.     Blood sample collection   1. (common serum tube) - red head cover, no additive in the blood collection vessel, used for routine serum biochemistry, blood bank (before blood storage, it is necessary to test various indicators of blood to determine whether it is qualified, the additive will affect the test results, so there is no additive in the blood collection vessel) and serological related tests.   2. (rapid serum tube) - orange red head cap, coagulant in the blood collection vessel, which can activate fibrin, change soluble fibrin into insoluble fibrin polymer, and then form stable fibrin clot. The rapid serum tube can coagulate the collected blood within 5 minutes, which is suitable for emergency serum series test.   3. (Inert separation gel coagulation accelerator tube)-golden head cap, inert separation gel and coagulant added in the blood collection tube After the specimen is centrifuged, the serum separating gel can completely separate the liquid components (serum or plasma) and the solid components (red blood cells, white blood cells, platelets, fibrin, etc.) in the blood and completely accumulate in the center of the test tube to form a barrier. The specimen is within 48 hours keep it steady. A coagulant can quickly activate the coagulation mechanism and accelerate the coagulation process, and is suitable for emergency serum biochemical tests.   4. (heparin anticoagulant tube) - green cap, with heparin added into the blood collection vessel. It is suitable for hemorheology, red blood cell fragility test, blood gas analysis, hematocrit test and ordinary biochemical test. Heparin has the function of antithrombin and can prolong the clotting time of samples, so it is not suitable for hemagglutination test. Excessive heparin can cause leukocyte aggregation and cannot be used for leukocyte count.   5. (plasma separation tube) - light green head cover, adding heparin lithium anticoagulant in inert separation tube, can achieve the purpose of rapid separation of plasma, is the best choice for electrolyte detection, can also be used for routine plasma biochemical determination and ICU and other emergency plasma biochemical detection. The plasma samples can be directly put on the machine and kept stable for 48 hours under cold storage.   6. (EDTA anticoagulant tube) - Purple head cap. The anticoagulant is ethylenediaminetetraacetic acid (EDTA). It can combine with calcium ion in blood to form chelate, which makes Ca2 + lose coagulation and prevent blood coagulation. It is suitable for many kinds of hematological examination. However, EDTA can affect platelet aggregation and is not suitable for coagulation test, platelet function test, calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and PCR test.   7. (sodium citrate coagulation test tube) - light blue head cap. Sodium citrate acts as anticoagulant mainly by chelating with calcium ions in blood samples. It is suitable for coagulation test.     8. (sodium citrate ESR test tube) - black head cover. The concentration of sodium citrate required for ESR test is 3.2% (equivalent to 0.109 mol / L), and the ratio of anticoagulant to blood is 1:4.   9. (potassium oxalate / sodium fluoride) - gray head cover, sodium fluoride is a weak effect anticoagulant, usually combined with potassium oxalate or sodium ethyliodate, its ratio is 1 phr sodium fluoride, 3 phr potassium oxalate, it is a good preservative for blood glucose determination, can not be used for urea determination by Urease method, nor for alkaline phosphatase and amylase determination, it is recommended for blood glucose detection.   Handling and storage   The treatment of blood samples needs to be carried out according to the plan or the central laboratory manual. In this link, special attention should be paid to the different treatment conditions of blood samples, such as: blood routine can not be centrifuged; blood that can be stored for a long time, such as PK blood, must record the temperature record of the refrigerator, and always record the temperature log for all blood samples to ensure that the refrigerator thermometer is within the validity period

Biological buffers may be chemical substances that we know little about but are extremely important for our survival, whether acetic acid and sodium acetate, sodium bicarbonate and carbonic acid, sodium dihydrogen phosphate and disodium dihydrogen phosphate exist in our body, or Tris and other buffers used in biological experiments, or HEPES buffer are added in chemical experiments to control reactions. They are used in another field The world helps us. Today, Desheng Xiaobian brings you to know the ubiquitous biological buffer.   Package of Desheng biological buffer in big barrel   No.1-Trometamol Tris is the most widely supplied biological buffer in the market, and it is also an important pharmaceutical intermediate, API and so on. The annual consumption is calculated by 10000 tons.   No.2-bicine (n, n-dihydroxyethylglycine) Bicine is a very important buffer for low temperature biochemical environment, which can be used to prepare stable substrate solution. It is one of the few buffers that can be used in special environment.   No.3 HEPES (4-hydroxyethyl piperazine ethanesulfonic acid) Nontoxic and harmless biological buffer, which can keep constant pH for a long time, is loved by cell Culturers. It is also widely used in the field of cosmetics.   No.4 caps (3 - (cyclohexylamine) - 1-propanesulfonic acid) Commonly used biological buffers, the most common place is in a variety of detection kits, such as biochemical diagnostic kits, DNA / RNA extraction kits, which are commonly used as buffers. In addition, its application in nucleic acid extraction and water-based coatings market can not be underestimated.   No.5-mops (3-morphine propanesulfonic acid) Mops can not form complexes with most metal ions, so it is suitable for buffer in solution containing metal ions. Mops is commonly used in the culture medium of bacteria, yeast and mammalian cells. Mops can also be used as running buffer in electrophoresis and buffer in protein purification chromatography.     As an enterprise integrating R & D, production, sales and service, Desheng is committed to providing customers with high-quality products and services Tris, bicine, HEPES, caps, mops, etc. can meet the special requirements of R & D customers for product types, packaging and purity. Desheng adheres to its own brand operation and has rich experience in the production and R & D of biological buffers. If you need to know more about biological buffers, please visit the official website of Desheng technology, website: wwww.whdsbio.com 。

TOOS, the full Chinese name of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt, is a widely used color reagent, and is most commonly used in the new Trinder's reagent. TOOS is used in many clinical biochemical testing projects. This article will introduce which testing projects the color reagent TOOS can be used for.   Precautions for using TOOS   TOOS is reductive. Although peroxidase is needed for detection, the enzyme is its catalysis. Without enzyme, it may be slowly oxidized by the oxygen in the air after being prepared into a solution. . The chromogen substrate needs to be prepared into a solution when used. The chromogen substrate produced by Desheng is a pure white powder, sealed and stored. After the solution is prepared, it should be prepared for immediate use. It will be slowly oxidized and discolored if it is in contact with air for a long time.     The reaction principle of TOOS detecting uric acid   Uric acid + oxygen + water are catalyzed by uricase to generate allantoin + carbon dioxide + water; two molecules of water + 4-aminoantipyrine + TOOS are catalyzed by oxidase POD to generate quinone imine + four molecules of water. From here, you can see the ratio of TOOS to uric acid reaction, and then through the reference value of the uric acid result, you can estimate the amount of TOOS and the concentration range of the configuration solution   The concentration of TOOS used in biochemical testing   For different biochemical tests, the concentration is different, but if the test volume is the same, the concentration difference is not big. The concentration of the coloring substrate TOOS is usually about 1~5mmol/L, according to the molecular weight of 295.33, when preparing the solution , You only need to add 0.3~1.5 grams of TOOS per kilogram of water, and the amount required is relatively small.   What testing items can TOOS be used for   TOOS can be used in diagnostic reagents for blood glucose testing, routine liver function tests, blood lipid testing items such as triglycerides, and cholesterol testing. It is of great value in clinical diagnosis. TOOS not only has high sensitivity, but also has high absorption wavelength, color reaction intensity, and Good performance in terms of fading.   The TOOS chromogenic substrate developed by Desheng Technology has the characteristics of high purity, high water solubility, and less interference impurity ions. It is ready to use when used, which is very convenient; our TOOS has a better crystal shape than the current products on the market. , Crystal Yan The color is more pure and white, and the quality and service are widely praised!