China Wuhan Desheng Biochemical Technology Co., Ltd Company News

TRIS stands for Tris(hydroxymethyl)aminomethane, white crystalline particles in appearance, CAS number is 77-86-1. As the most commonly used raw material for buffer preparation, TRIS base is widely used in biochemistry and molecular biology experiments. In addition, TRIS is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Because it has three equal hydroxyl groups, it can also be used as a good reducing agent. Under the alkaline condition of sodium hydroxide, the chloroauric acid solution can be reduced to prepare stable gold nanoparticles. This method is very environmentally friendly, non-toxic and harmless, and can be called green magic. The more classic methods for synthesizing gold nanoparticles are sodium citrate reduction method, phase transfer method and seed growth method. These methods are very easy to modify surface biomolecules on gold nanoparticles, but the preparation and modification processes of these methods have certain toxicity. In order to reduce the toxicity of gold nanoparticles to biological organisms in experiments, and enable them to obtain better applications in the field of biomedicine, in recent years, scientific researchers have been committed to continuously developing new green reduction methods. Under alkaline conditions, the chloroauric acid solution is reduced by ultrasound without adding other stabilizers, and relatively stable gold nanoparticles can be prepared. The synthesis of environmentally friendly and biocompatible gold nanoparticles is realized. By adjusting the experimental conditions, gold nanoparticles of different sizes can be prepared. The preparation of gold nanoparticles by TRIS ultrasonic method is a new green and environmentally friendly reduction method. Compared with other green synthesis methods, the experimental conditions are milder and the operation is simple and easy. This provides new research ideas and theoretical basis for the synthesis of gold nanoparticles with green pollution-free, low toxicity, low cost, and high biocompatibility. Hubei New Desheng Materials specializes in the production and development of biological buffers and blood collection tube additives, enzyme preparations, chemiluminescence reagents, luminescent substrates and other products. Desheng has been established for decades and has its own R&D team. It has been researching and developing biological buffers for many years. Its products include a series of biological buffers such as TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, and PIPES. At present, the products produced by Desheng have been sold to many countries around the world, and they have been repurchased with praise, and have reached long-term cooperation with many companies. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.

Sodium citrate is a widely used chemical additive in food, medical and chemical industries. Widely used in medicine and other industries. However, for some people who are new to this product, they are not clear about the requirements of sodium citrate in different application fields. For example, recently we have contacted a purchaser from a daily chemical product factory. The sodium citrate he needs and the trisodium citrate dihydrate we use as an anticoagulant in blood collection tubes are completely different products and cannot be replaced by each other. Sodium citrate is usually referred to as trisodium citrate. The full name is actually called trisodium citrate dihydrate. The molecular formula is Na3C6H5O7.2H2O. This substance is widely used in food additives, emulsification enhancers and the pharmaceutical industry. Colorless crystals or white crystalline powders, taste salty, and have a unique cooling sensation. In addition to trisodium citrate dihydrate, there is also trisodium citrate pentahydrate with the molecular formula Na3C6H5O7·5.5H2O. The difference is only the number of crystal water. Sodium citrate is often used as an anticoagulant in medical and health care. Sodium citrate can form difficult-to-dissociate complexes with calcium ions in the blood to prevent calcium ions from participating in the coagulation process. This is the main product of Desheng Biochemical Sodium Citrate field. Like EDTA Na2 and potassium oxalate, it is a good coagulant in blood collection tube additives. In clinical collection of fresh blood, it is necessary to add a certain amount of sterilized sodium citrate as an anticoagulant. The citric acid generated by the ionization of sodium citrate can combine with calcium ions in the blood to form complexes that are difficult to ionize, thereby inhibiting The coagulation of blood, so sodium citrate is called an anticoagulant. It can also be used as a diuretic and phlegm-reducing medicine. Sodium citrate has many excellent properties: such as safety and non-toxicity, biodegradability, strong complexing power for metal ions, excellent solubility, and good pH adjustment ability. It should be noted in the pharmaceutical industry that citrate ions and calcium ions can form a soluble complex that is difficult to dissociate, thus reducing the concentration of calcium ions in the blood and hindering blood coagulation. This product is used as an in vitro anticoagulant during blood transfusion or laboratory blood sample anticoagulation. In the formation of prothrombin activator and the subsequent clotting process, calcium ions must be involved. Desheng Biochemical has specialized in the production of sodium citrate since 2005. I would like to remind you that sodium citrate has a wide range of uses, including analytical grade, industrial grade and medical grade. According to the needs of special industries, special attention should be paid to the purity, the content of crystal water, and the packaging and storage methods. If you have any questions about the use of the product, please contact Desheng Biochemical for consultation.

Biological buffer is a kind of solution that can keep the pH value of the solution relatively stable when adding a small amount of acid/base. The pH range of the biological buffer is determined by its pKa value. The pKa value is defined as the pH value when 50% of the molecules have an acid structure and 50% have a basic structure. A general principle about biological buffers is that the pH value should be within 1 pH unit around the pKa value, so as to ensure that it has a good buffering capacity. In this way, it can be ensured that there are enough molecules existing in the acid structure and the basic structure at the same time to neutralize them when H+ or OH- is added. In this way, the buffer can prevent changes in protein stability caused by changes in pH. Many substances can be used in biological buffers. The frequently used buffer components usually have a pKa value close to neutral and can be used around the physiological pH range. The following will list the 3 most commonly used biological buffers, their respective pH application ranges, and their advantages and disadvantages that may affect the protein purification process. To ensure sufficient buffering capacity, the concentration of these buffers is usually 25mM. 1. MOPS buffer solution: PH range is 6.5-7.9, can not be autoclaved, PH value changes with temperature to a certain extent, and has high buffering capacity within the physiological PH value range. 2. HEPES buffer solution: PH range is 6.8-8.2, can not be autoclaved, PH value changes with temperature to a certain extent, and free radicals will be generated under certain conditions. 3. TRIS buffer: PH range is 7.5-9.0. PH value changes with temperature or degree of dilution to a certain extent, which will affect some enzyme activities. It has no ultraviolet absorption and is cheap. The function of the buffer solution is to adjust the pH value of the solution within a limited range so that the acidity of the solution to be tested meets the range specified by the analytical method. For example, phenol can be combined with 4-aminoamino acid in the presence of alkaline medium and potassium ferricyanide. Tipyrine reacts to produce orange-red antipyrine dye. In order to prevent the interference of aromatic amines, the most suitable pH is 10±0.2. For this reason, it is necessary to add ammonia-money chloride buffer solution to the solution to be tested to adjust and control the pH of the solution to be tested to 10.0±0.2. Hubei New Desheng Materials specializes in the production and development of biological buffers and blood collection tube additives, enzyme preparations, chemiluminescence reagents and luminescent substrates. Desheng has been established for decades and has its own R&D team. It has been researching and developing biological buffers for many years. Its products include a series of biological buffers such as TRIS base, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, and PIPES. At present, the products produced by Desheng have been sold to many countries around the world, and they have been repurchased with praise, and have reached long-term cooperation with many companies. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.

The buffer range of Tris is about 6-8, which is suitable for most biochemical experiments, especially in the related research experiments of protein and nucleic acid; EPPS buffer is also suitable for protein and nucleic acid. The research experiment is more expensive than Tris, so which one is suitable for the Folin phenol method to detect protein experiment? The Folin-phenol reagent method is a basic method commonly used to determine protein concentration and has been widely used in the field of biochemistry. In practical applications, EPPS (HEPPS) is usually used as a buffer for Folin phenol method to detect protein content, rather than using Tris buffer. Buffer Tris and EPPS are used for protein content detection The coloring principle of the Folin phenol method for the determination of protein content is the same as that of the biuret method (but EPPS cannot be used for biuret/biuret detection). The difference is that the second reagent, the Folin-phenol reagent, is added. In order to increase the amount of color, thereby improving the sensitivity of detecting proteins. The advantage of the Folin phenol method for detecting protein content is that it has high sensitivity and is much more sensitive than the biuret method. Of course, it also has some disadvantages that it takes a long time, the standard curve is not straight, the specificity is poor, and there are more interfering substances. Any group that interferes with the biuret reaction, such as -CO-NH2, -CH2-NH2, CS-NH2 and Tris buffer, sucrose, ammonium sulfate, and sulfhydryl compounds, can interfere with the Folin-phenol reaction and affect the latter Much bigger. In addition, phenols and citric acid also interfere with this reaction. Folin-phenol reagent consists of two reagents. Reagent one consists of sodium carbonate, sodium hydroxide, copper sulfate and potassium sodium tartrate. The peptide bond in the protein reacts with the potassium sodium copper tartrate solution under alkaline conditions to form a purple-red complex. The second reagent is composed of phosphomolybdic acid, phosphotungstic acid, sulfuric acid, bromine and so on. Under alkaline conditions, this reagent is easily reduced by the phenolic group of tyrosine in the protein to give a blue reaction, and its color depth is proportional to the protein content. This method is also suitable for determining the content of tyrosine and tryptophan. The measurement range of this method is 25-250μg protein. In summary, the advantage of the Folin phenol method for determining protein content is high sensitivity, but the disadvantage is that it takes a long time and interferes. Tris can also cause interference. EPPS buffer should be used. Desheng produces both of these biological buffers, not to say which one is better, but to be suitable for different experimental studies.

Carbomer is a white, loose powder with strong hygroscopic properties, soluble in water, ethanol and glycerin. It can be used as topical lotion, gel, cream. The overall carbomer gel system of neutral Ph under 7 environment is an excellent gel matrix, with crystal clear appearance, smooth and moisturizing touch, suitable for cream or gel preparation. At the same time, due to its simple addition process, it is easy to use. It feels comfortable afterwards, and is used in partial administration, especially in skin and eye gels. It has a wide range of applications. Although it can be combined with water to form a gel-like transparent milky alternation, it is not directly soluble in water like traditional inorganic salts. Today, we will introduce an alkaline preparation method to prepare an ideal carbomer gel solution. . First weigh 1g of Carbomer 940 dry powder in a 50ml dry beaker. If you encounter agglomerates, please use a spoon to crush it. Then add 25ml of purified water, stir while adding, so that Carbomer 940 fully absorbs water and swells. Then you have to let the Carbomer 940 fully absorb water. Generally, after adding water and stirring, leave it for a few hours before the Carbomer 940 can fully swell. If you want to go faster, you can heat it in a water bath. While heating and stirring, it will form faster. Even slow heating can accelerate the water absorption and swelling process of Carbomer 940, and it takes 10 to 30 minutes. . Then use a dropper to add the lye, stirring while adding, and measuring the pH value. When the pH is adjusted to 7, the gel is well prepared. (Triethanolamine can be used for lye). At the same time make up the remaining amount of water. The total volume is maintained at 50ML. The formulated gel base can be directly or indirectly mixed with other raw materials to prepare various gels, as long as the carbo concentration is controlled to be no more than 0.8%, and it is more suitable to be about 0.5%. Only 0.5% ingredients are needed to make a gel product with good appearance and texture. It is like making a 100ml gel. Take 25g of a pre-prepared 2% polymer gel and add it to a 75ml formula. 100ml of 2% transparent gel contains the following components, decomposed as follows: 2gm gel powder + 98ml pure water + 0.5ml antibacterial agent + appropriate amount of neutralizer. DIY Carbomer 940 gel. Need special reminder: Don't add anything indiscriminately! Such as soluble salts, very acidic solutions (lemonade, vinegar). The carbomer series 940 gel system becomes thin immediately. Although hyaluronic acid is also acidic, the added amount is small, which has little effect on the stability of the carbomer 940 gel system, so feel free to add it. Pure dew is slightly acidic, and the effect is not significant after adding. Carbomer 940 itself has no nutrition, does not support the growth of bacteria and molds, but does not prevent bacteria and molds, and uses the nutrients present in the gel system to promote their growth. So don't mix too much at one time, add preservatives or essential oils as needed. )Ultraviolet rays have a certain effect on the Carbomer 940 gel system, so please keep it away from light. When formulating skin care products, it is appropriate to control the final concentration of Carbomer 940 not to exceed 0.8%. It is recommended that the concentration be around 0.5%. If the concentration exceeds 0.8%, it is easy to form film. Personally, I think the recommendation is about 0.7 is more beautiful, and the concentration is too thin, which belongs to another category-a thicker essence. Desheng Biochemical was founded in 2005 and has been specialized in the R&D, production and sales of Carbomer 940/980 for many years. On the basis of many years of practice, independent intellectual property rights and professional R&D capabilities have been formed and produced. Provide products and raw material solutions for domestic and foreign daily-use washing and cosmetics manufacturers.

When it comes to carbomer, most people don't talk about it often, but carbomer is used in many cosmetics. Even though they may benefit from carbomer every day, most people don't know what carbomer is. Carbomer is not like an active ingredient that provides a result-oriented product, but an inert ingredient that can help these active ingredients play an outstanding role. Carbomer describes a series of polymers made of acrylic acid. It is a white fluffy powder, but it is often used as a gel in various cosmetics and personal care products. These cosmetics and personal care products are used in skin, hair, nails and makeup products and dentifrice. Carbomer is also commonly listed as Carbomer 934, Carbomer 934P, Carbomer 941, Carbomer 910 and Carbomer 910. Thicken or homogenize the consistency of the product Carbomer is a thickening agent that helps control the consistency or viscosity and fluidity of many cosmetics. For the hands that need to be disinfected during the journey, the natural hand sanitizer gel is 99.9% effective in fighting common bacteria, and its formula will not make your hands feel sticky or dry.   Prevent product separation In addition, they help to disperse and suspend insoluble solids in liquids and prevent oil and liquid parts from separating in the solution. This useful property is what makes thinner moisturizers such as emulsions work.   Improve the texture of the product Carbomer can easily absorb and retain water, and can greatly expand when suspended in water, up to 1000 times the original volume. By adding carbomer to shampoo, conditioner, cream and lotion, these The formula will appear richer, smoother and creamy.   Delay aging and reduce skin cracking Peptide eye gel, due to the presence of carbomer, it provides the moisturizing properties of squalane and the anti-aging effect of peptides in the gel formulation. Use a gel-like consistency. Use Aloe Vera in Gel Aloe 95 to help moisturize your skin. Aloe vera has many skin healing properties, such as reducing inflammatory acne, preventing fine lines and wrinkles, and when dandruff is used on the scalp, it can also Help solve hair problems such as dandruff.   Desheng Biochemical is a manufacturer specializing in carbomer, supplying carbomer 940/980 all year round. Welcome to consult

Decoration has always been a hot topic. Because it is inseparable from our lives. Especially for our own home decoration, we always need to consider many issues. Because home is a warm and comfortable place for everyone to enjoy, but in order to save costs, many decoration companies are not very satisfactory in the choice of paint. Therefore, choosing a good decoration material is also a problem we should pay attention to. In recent years, water-dispersible polyisocyanates have shown importance in various application fields. At present, water-dispersible polyisocyanates in most applications are modified by polyethers to achieve non-ionic hydrophilic modification. Although this type of hydrophilic modified polyisocyanate has been widely recognized in the market in most applications, it also has certain defects, such as its high viscosity, which requires considerable shearing force to be uniformly distributed during the construction process. Introduced into the aqueous medium. In order to avoid the above shortcomings, this also gives CAPS buffer an excellent opportunity. Let it find its place in the new paint. General ion-modified groups include carboxyl, sulfuric acid, hydroxyl, hydroxysulfonic acid, etc., but there are still certain defects, such as carboxyl modified polymers are prone to gelation, and the color of hydroxysulfonic acid modified products Obviously yellowing etc. Later studies found that CAPS-modified polyisocyanate can be finely dispersed in water, and the product is stable in storage. CAPS-modified polyisocyanates show certain advantages over other ionic or non-ionic modified products. 3-(Cyclohexylamino)-1-propane sulfonic acid (CAPS) reacts with aliphatic polyisocyanate (the former is zwitterionic sulfamate) under mild conditions and in the presence of a tertiary amine neutralizer to generate sulfonic acid Urea derivatives are excellent emulsifiers. Regardless of salt-forming groups, CAPS-modified polyisocyanates have good storage stability and are not turbid. Even when they contain less sulfonate groups, a well-dispersed emulsion can be obtained in water. A series of ionized modified polyisocyanates can be obtained, which can be used in various environmentally friendly high-quality water-based two-component polyurethane coatings. These coatings are completely comparable to general solvent-based coatings in terms of dryness, curing and chemical resistance. The new regulations require further reduction of VOC (Volatile Organic Compounds). In the future, the amount of these crosslinking agents will be greatly increased. Compared with solvent-based coatings, they will not cause the quality of the paint film to decrease. The use of CAPS modified formulation coating saves a lot of trouble in our decoration. Without the hassle, it is natural whether we live or use it everywhere. In addition to the use of CAPS in new coatings, it also has a wide range of uses as a biological buffer, used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. The CAPS produced by Desheng Company is reagent grade with a purity greater than 99%. It can not only be used as a buffer for in vitro diagnostic reagents, but also can be used in coatings. Customers in need can contact us, and we will provide samples for your testing.

Accurately locate the single product of biological buffer you need Biological buffers have a very wide range of applications in the field of biochemical engineering, but we don't know much about it. Now we Desheng take you to understand the biological buffers we produce: Tris, BICINE, HEPES, CAPS, MOPS, etc. are used where and where are their advantages, so that you can clearly position when buying products. To the product you need, and help you get a general understanding of the product. NO.1 tris (Tris(hydroxymethylaminomethane) or tromethamine) Used in the preparation of buffers in biochemistry and molecular biology experiments; pharmaceutical intermediates; used in the preparation of surfactants and vulcanization accelerators; preparation of water-soluble polymers containing Tris structural units as coating dispersants; indoor formaldehyde adsorption Preparation of materials, etc. NO.2 Bicine (N,N-dihydroxyethyl glycine) Bicine is a very important buffer suitable for low-temperature biochemical environments. It can be used to prepare stable substrate solutions. It is one of the few buffers that can be used in special environments. NO.3 HEPES (4-hydroxyethylpiperazine ethanesulfonic acid) A non-toxic and harmless biological buffer, which can maintain a constant pH for a long time, and is loved by cell culturers. NO.4 CAPS (3-(cyclohexylamine)-1-propanesulfonic acid) In the application of nucleic acid hybridization, CAPS can reduce the yield of non-specific hybridization products and increase the specificity of nucleic acid hybridization; at the same time, it can maintain the yield of target hybridization products; it is useful in the identification of specific pathogens, the diagnosis of genetic diseases, and the analysis of gene sequences. Important value. It is commonly used as a buffer in detection kits, such as biochemical diagnostic kits and DNA/RNA extraction kits. It can also be used in a variety of environmentally friendly high-quality water-based two-component polyurethane coatings, and can be used in water-based isohydrogen ester curing agents. It can be combined with active groups (hydroxyl, carboxyl, amino, epoxy, etc.) Resin coexists stably for a long time. NO.5 MOPS (3-morpholinopropane sulfonic acid) MOPS buffer does not form complexes with most metal ions, and is suitable for buffers in solution systems containing metal ions. It can be commonly used in the culture medium of bacteria, yeast and mammalian cells. It is also used as a running buffer in electrophoresis and a buffer in protein purification chromatography.

Vacuum blood collection tubes are an important tool for virus detection in hospitals and play a pivotal role in the field of medical and health care. She is an important blood test equipment and apparatus. Additives for blood collection tubes are a key factor affecting the function of blood collection tubes. Commonly used additives are anticoagulants, coagulants, buffers, protective agents, separating gels, etc. This article will introduce several common blood collection tube anticoagulants and their effects. Anticoagulant, as the name implies, is a chemical agent that prevents blood from clotting. Commonly used anticoagulants for blood collection tubes are heparin sodium, heparin lithium, sodium citrate, potassium oxalate and ethylenediaminetetraacetic acid (EDTA). Heparin is considered to be the best anticoagulant in the determination of blood chemical composition. Sodium and lithium salts are commonly used in clinical blood tests and have unique application value. Heparin is mainly found in blood collection tubes with green caps, and is suitable for red blood cell fragility test, blood gas analysis, hematocrit test, blood rheology and emergency biochemical determination. Lithium heparin has the least possibility of interference when detecting non-lithium ions, and has better anticoagulant activity than heparin sodium. At present, heparin lithium is gradually replacing heparin sodium in blood tests. Sodium citrate, citrate can form a soluble chelate with calcium ions in the blood, thereby preventing blood clotting. It is generally used for checking blood coagulation and blood cell deposition rate. Sodium citrate is used as an anticoagulant in anticoagulant tubes with light blue caps and ESR tubes with black caps. Potassium oxalate is an anticoagulant with high dissolution concentration and strong anticoagulant effect. The principle of action is that oxalate and blood calcium ions form calcium oxalate precipitation and tissue coagulation. It is often used for anticoagulation of blood samples for testing. Potassium oxalate is often mixed with sodium fluoride, and it is present in the gray cap anticoagulation blood collection tube. Ethylenediaminetetraacetic acid (EDTA) is one of the most commonly used and most important anticoagulants and reagents in clinical examinations. EDTA can combine with calcium ions in the blood to form a chelate, and Ca2+ loses the coagulation effect, thereby preventing blood coagulation. It is suitable for a number of hematological examinations. The commonly used EDTA anticoagulant is EDTA-2K. The EDTA purple tube cap anticoagulant blood collection tube is suitable for whole blood and plasma testing. It is the first choice for blood routine, glycosylated hemoglobin, and blood group testing. In summary, anticoagulants play an important role in blood vessel detection, but not all blood collection tubes contain anticoagulants. In blood collection tubes of different colors, green, blue, black, gray and purple caps are used in blood collection tubes. Contains anticoagulant, EDTA-2K is mainly used in purple anticoagulant tube. Desheng Biotech specializes in the production of blood collection tube additives and has 15 years of production and research and development experience. Committed to providing complete blood testing solutions for hospitals, clinics and scientific research institutions across the country. Contains a series of products such as EDTA, lithium heparin, and anti-irradiation gel.

In vitro diagnostic reagents are widely used in medical research and clinical testing, and are an important indicator of the quality of clinical diagnostic information.  In-vitro diagnostic reagents generally refer to products and services that are outside the human body, and obtain relevant clinical diagnostic information by testing the body, including blood, body fluids, and tissue samples, which can help judge diseases or body functions. The stability of in vitro diagnostics is an indicator that guarantees its quality and accuracy of test results. It is an essential attribute of reagents and an important reference for the production, transportation, storage and use of in vitro diagnostic reagents. In vitro diagnostic reagent stability tests usually include real-time and actual stability, accelerated stability, bottle opening or unsealing stability, reconstitution stability, sample stability, transportation and stability, and reagent and sample storage stability. The purpose of these stability studies is to determine the transportation, storage and storage conditions of reagent products after opening, and to determine the shelf life of the product and the shelf life after opening. In addition, it can also verify that the stability of the product changes when the storage conditions and shelf life are changed, so as to evaluate and adjust the product's composition, process, and packaging materials based on the results. Take the index of actual and sample storage stability as an example. This index is one of the important factors that affect the effect of in vitro diagnostic reagents. The storage of reagents should be placed and stored in strict accordance with the instructions. For example, for freeze-dried powder reagents containing peptides, the water content and oxygen content in the storage environment have a great influence on the stability of the reagents. Therefore, unopened freeze-dried powders should be stored in a refrigerator in a refrigerator as much as possible. Samples to be tested, such as samples processed by medical institutions after collection, should be stored as required according to their performance and risk factors. During routine blood tests, the blood and anticoagulant should be shaken and the samples should be placed at room temperature (about 20°C). ) After 30 minutes, 3h, and 6h, the machine will be tested. Some special samples, such as nasopharyngeal swab samples collected during the nucleic acid test of the new coronavirus, need to use a virus sampling tube containing a virus preservation solution, and the specimens used for virus isolation and nucleic acid testing should be tested as soon as possible, within 24 hours The tested specimens can be stored at 4°C; the specimens that cannot be tested within 24 hours should be stored at -70°C or below (if there is no storage condition of -70°C, they should be temporarily stored in the refrigerator at -20°C). Hubei New Desheng Materials specializes in the production and development of in vitro diagnostic reagents, chemiluminescence reagents, luminescent substrates, biological buffers, blood collection tube additives and enzyme preparations. Desheng has been established for decades and has its own R&D team. It has been researching and developing in vitro diagnostic reagents for many years. It has many kinds of chemical reagent products under its umbrella. At present, the products produced by Desheng have been sold to many countries around the world, and they have been repurchased with praise, and have reached long-term cooperation with many companies. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.

Biological buffers are often used in biochemical research experiments and are of extremely important significance. They are a kind of conditioning fluid that can resist the influence of a small amount of strong acid and alkali from the outside and maintain the pH value basically unchanged. Among the commonly used biological buffers are Tris buffer solution, PBS buffer solution, CAPS buffer, MOPS buffer, TAPS buffer and EPPS buffer. This article will compare and introduce the most commonly used Tris buffer solution and PBS buffer solution from several aspects. 1. Buffer range Tris is a weak base with a molecular formula of C4H11NO3, a molecular weight of 121.14, and a pKa of 8.1 at 25 °C. The effective buffer range of its buffer is between pH 7.0 and 9.2. The pH values ​​commonly used in biochemical experiments are 6.8, 7.4, 8.0, and 8.8. Tris is widely used in the preparation of buffers in biochemistry and molecular biology experiments, and even has a tendency to exceed phosphate buffers. Phosphate buffered saline (PBS) is a salt solution with phosphate as the pH buffer and sodium chloride as the osmotic pressure balance. Commonly used are sodium phosphate buffer and potassium phosphate buffer. Because of their secondary dissociation and a wide range of buffering pH values, they are the most widely used buffer solutions in biochemical research. 2. Configuration method There are two ways to prepare Tris-HCl buffer: prepare Tris and HCl solutions separately, and then mix according to the volumes listed in the common table. However, because standard concentration of dilute hydrochloric acid is not easy to prepare, another method is commonly used: Take the configuration of Tris-HCl buffer as an example: first dissolve Tris base in 950 mL～970 mL deionized water, add HCl dropwise while stirring, and use The pH meter measures the pH value of the solution to the required pH value, and then adds water to it. The preparation method of phosphate buffer solution: Weigh NaCl, KCl, KH2PO4 and K2HPO4, dissolve them in 800 mL of distilled water, adjust the pH of the solution to 7.4 with HCl, and finally add distilled water to make the volume to 1 L. It can be stored in a refrigerator at 4 ℃. It should be noted that the usual concentration refers to the concentration of all phosphates in the buffer solution, not the concentration of Na+ or K+. Na+ and K+ are only used to adjust the osmotic pressure. Potassium salt is better than sodium salt when configuring phosphate buffer solution. Sodium salt is not easy to dissolve at low temperature, while potassium salt has relatively higher solubility. 3. Field of Use The Tris buffer solution forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH; the Tris-HCl buffer system is used in the gel to stabilize the pH; it is widely used as a solvent for nucleic acids and proteins; the low ionic strength of the Tris buffer is also It can be used to form the intermediate fibers of nematodes; add EDTA to Tris hydrochloric acid buffer to make "TE buffer", which can be used for DNA stabilization and storage; change the pH-adjusting acid solution to acetic acid to obtain "TAE buffer" , Change to boric acid to get TBE buffer. These two buffers are often used in nucleic acid electrophoresis experiments. Phosphate buffer solution Generally active biological preparations must be diluted with phosphate buffer solution. The reason is that it has a salt balance and a buffering effect that can adjust the appropriate pH value. Distilled water has no salt balance effect, which will destroy the structure and biological properties of biological proteins; physiological saline does not have the effect of adjusting pH, and it cannot ensure that it participates in biological reactions under optimal conditions for complete and active substances, so the use of PBS is Preferred. Of course, PBS is not a panacea. Some biologically active substances require relatively high conditions, and more ingredients need to be added to the balance buffer to maintain the best conditions to ensure that the biologically active substances maintain their most complete characteristics. Therefore, PBS is mainly used for cell experiments, and its buffer range is most suitable for neutral. In the biochemical experiment, the buffer solution should be carefully selected, not only the buffer range of the buffer solution, but also the use environment of the buffer solution should be considered comprehensively. Hubei New Desheng Materials has been specializing in the production and development of biological buffers for many years. Desheng has an independent R&D team and has a wealth of practical experience in product development and production. At present, the biological buffers and other products produced by Desheng have been sold to many countries around the world, and they have been repurchased with praise, and have reached long-term cooperation with many foreign customers. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.

With the development of science and technology and the emergence of various advanced instruments, clinical biochemical projects have put forward higher requirements for blood collection and separation. It not only requires rapid results, but also requires us to ensure the accuracy of the results of each project. . In the past, hospitals used ordinary drying tubes to separate blood samples. Recently, due to the development of related technologies, many hospitals have begun to use lithium heparin anticoagulation tubes and separation gel accelerator tubes to separate blood samples, and analyze their plasma (serum) after centrifugation. What are the advantages of these two types of vacuum blood collection tubes? Blood is collected in the morning and tested in the laboratory. Generally, it should be placed at room temperature for 2-3 hours. During this period, the metabolic activity of the cells does not stop. As the storage time increases, the intracellular and extracellular substances transfer. Serum separation gel is a viscous fluid with a specific gravity of 1.05, which is between serum (1.02) and blood clot (1.08). Its structure contains a large number of hydrogen bonds. The association of hydrogen bonds forms a network structure. The viscous liquid of the network structure is thixotropic. When the separating gel and the coagulated blood are centrifuged in the same test tube, under the action of centrifugal force, the network structure is destroyed and becomes a low-viscosity fluid, causing a rotation phenomenon. The blood clot heavy by the separating gel is transferred to the bottom of the test tube, and the separating gel forms a gel-like isolation layer between the serum and the blood clot, blocking and reducing the passage of blood cells to affect the serum components, thereby stabilizing the substances in the serum. The anticoagulation of the lithium heparin tube can maintain the integrity of cells, delay the precipitation of the myocardial enzyme spectrum in the red blood cells, and the separation rubber tube can quickly accelerate the coagulation to release a part of the myocardial enzyme spectrum from the blood cells. After centrifugation, the separation of blood cells and serum has a certain relationship. Therefore, the determination of myocardial enzyme spectroscopy is greatly affected by the precipitation of substances in red blood cells. The separation hose and the lithium heparin tube can precipitate serum or plasma quickly, reducing errors caused by sample placement, less hemolysis, less impact on the results, and more realistic results, etc. advantage. Relatively speaking, the separation hose separates blood cells and serum so that the result is closer to the situation at the time of blood collection. Therefore, when conditions permit, we should use separation hoses or lithium heparin tubes to dispense myocardial zymogram specimens as much as possible. If ordinary drying tubes are used to dispense myocardial zymogram specimens, serum should be separated as soon as possible after blood sampling.