What are the advantages and disadvantages of biological buffers
Biological buffer is a kind of solution that can keep the pH value of the solution relatively stable when adding a small amount of acid/base. The pH range of the biological buffer is determined by its pKa value. The pKa value is defined as the pH value when 50% of the molecules have an acid structure and 50% have a basic structure. A general principle about biological buffers is that the pH value should be within 1 pH unit around the pKa value, so as to ensure that it has a good buffering capacity. In this way, it can be ensured that there are enough molecules existing in the acid structure and the basic structure at the same time to neutralize them when H+ or OH- is added. In this way, the buffer can prevent changes in protein stability caused by changes in pH.
Many substances can be used in biological buffers. The frequently used buffer components usually have a pKa value close to neutral and can be used around the physiological pH range. The following will list the 3 most commonly used biological buffers, their respective pH application ranges, and their advantages and disadvantages that may affect the protein purification process. To ensure sufficient buffering capacity, the concentration of these buffers is usually 25mM.
1. MOPS buffer solution: PH range is 6.5-7.9, can not be autoclaved, PH value changes with temperature to a certain extent, and has high buffering capacity within the physiological PH value range.
2. HEPES buffer solution: PH range is 6.8-8.2, can not be autoclaved, PH value changes with temperature to a certain extent, and free radicals will be generated under certain conditions.
3. TRIS buffer: PH range is 7.5-9.0. PH value changes with temperature or degree of dilution to a certain extent, which will affect some enzyme activities. It has no ultraviolet absorption and is cheap.
The function of the buffer solution is to adjust the pH value of the solution within a limited range so that the acidity of the solution to be tested meets the range specified by the analytical method. For example, phenol can be combined with 4-aminoamino acid in the presence of alkaline medium and potassium ferricyanide. Tipyrine reacts to produce orange-red antipyrine dye. In order to prevent the interference of aromatic amines, the most suitable pH is 10±0.2. For this reason, it is necessary to add ammonia-money chloride buffer solution to the solution to be tested to adjust and control the pH of the solution to be tested to 10.0±0.2.
Hubei New Desheng Materials specializes in the production and development of biological buffers and blood collection tube additives, enzyme preparations, chemiluminescence reagents and luminescent substrates. Desheng has been established for decades and has its own R&D team. It has been researching and developing biological buffers for many years. Its products include a series of biological buffers such as TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, and PIPES. At present, the products produced by Desheng have been sold to many countries around the world, and they have been repurchased with praise, and have reached long-term cooperation with many companies. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.
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