Does the separation rubber tube and the lithium heparin tube affect the determination of myocardial enzymes?

With the development of science and technology and the emergence of various advanced instruments, clinical biochemical projects have put forward higher requirements for blood collection and separation. It not only requires rapid results, but also requires us to ensure the accuracy of the results of each project. . In the past, hospitals used ordinary drying tubes to separate blood samples. Recently, due to the development of related technologies, many hospitals have begun to use lithium heparin anticoagulation tubes and separation gel accelerator tubes to separate blood samples, and analyze their plasma (serum) after centrifugation. What are the advantages of these two types of vacuum blood collection tubes?

Blood is collected in the morning and tested in the laboratory. Generally, it should be placed at room temperature for 2-3 hours. During this period, the metabolic activity of the cells does not stop. As the storage time increases, the intracellular and extracellular substances transfer. Serum separation gel is a viscous fluid with a specific gravity of 1.05, which is between serum (1.02) and blood clot (1.08). Its structure contains a large number of hydrogen bonds. The association of hydrogen bonds forms a network structure. The viscous liquid of the network structure is thixotropic. When the separating gel and the coagulated blood are centrifuged in the same test tube, under the action of centrifugal force, the network structure is destroyed and becomes a low-viscosity fluid, causing a rotation phenomenon. The blood clot heavy by the separating gel is transferred to the bottom of the test tube, and the separating gel forms a gel-like isolation layer between the serum and the blood clot, blocking and reducing the passage of blood cells to affect the serum components, thereby stabilizing the substances in the serum.

The anticoagulation of the lithium heparin tube can maintain the integrity of cells, delay the precipitation of the myocardial enzyme spectrum in the red blood cells, and the separation rubber tube can quickly accelerate the coagulation to release a part of the myocardial enzyme spectrum from the blood cells. After centrifugation, the separation of blood cells and serum has a certain relationship.

Therefore, the determination of myocardial enzyme spectroscopy is greatly affected by the precipitation of substances in red blood cells. The separation hose and the lithium heparin tube can precipitate serum or plasma quickly, reducing errors caused by sample placement, less hemolysis, less impact on the results, and more realistic results, etc. advantage. Relatively speaking, the separation hose separates blood cells and serum so that the result is closer to the situation at the time of blood collection. Therefore, when conditions permit, we should use separation hoses or lithium heparin tubes to dispense myocardial zymogram specimens as much as possible. If ordinary drying tubes are used to dispense myocardial zymogram specimens, serum should be separated as soon as possible after blood sampling.