China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Blood testing is very important, it involves many items. Vacuum blood collection tube additives are used in the sampling process of blood testing, and their role is to maintain the original properties of the blood to ensure high-quality blood samples. Here is a brief introduction to the specific items of blood testing.   Blood biochemical test: 1. Liver function test, ALT, AST, GGT, ALP, TBil, DBILI, IBILI, TP, ALB, BIB, A/G, ADA, PAB, AFU, CHE, liver function+, TBA, LDH, CH, GPDA, NUT, MAO, GLDH, ASTm, protein electrophoresis, etc. The blood collection tube uses a red cap tube to self-coagulate or use a coagulant tube containing a coagulant.   2. Renal function test, EURA, CREA, U/C, UA, β2-MG, CC, etc. Use self-coagulating blood vessels or coagulant tubes for blood collection.   3. Blood lipid testing, TG, Chol, APOA1, HDL-C, LP(a), etc. Self-coagulating blood vessels or coagulant tubes are used for blood collection. Blood test 4. Myocardial enzyme detection, AST, CK, CK-MB, LDH, etc., use self-coagulating blood vessels or coagulant tubes.   5. Electrolyte detection, potassium, sodium, chlorine, calcium plasma, CO2, AG, etc., use self-coagulating blood vessel or coagulant tube.   6. Blood glucose and glucose tolerance test, blood sampling with grey cap anticoagulant tube.   7. Detection of glycosylated hemoglobin and hepatitis B indicators, using a purple cap anticoagulant tube.   Blood immune test: 1. Full tumor detection, tumor markers, gastrointestinal tumors, digestive tract tumors, lung cancer series, etc. Self-coagulating blood vessels or coagulant tubes are used for blood collection. 4 mL of blood is collected for all tumor items, 3 mL for other combined items, and 2 mL for single items.   2. Humoral immunity, IgG, IGA, IgM, C3, C4, etc., self-coagulating blood vessels or coagulant tubes for blood collection.   3. Rheumatism indicators, inflammation indicators, autoimmune liver antibody spectrum, autoantibody series, use self-coagulating blood vessels or coagulant tubes.   Professional blood test: 1. Blood cell analysis, reticulocyte count, purple cap tube, anticoagulant EDTA dipotassium tube.   2. Determination of red blood cell sedimentation rate, using black cap tube, 3.8% sodium citrate as anticoagulant, adding ratio 1:4 to blood collection volume.   3. Four items of coagulation, plasma prothrombin time, plasma fibrinogen, activated partial thromboplastin time and plasma thrombin time, using light blue cap blood collection tube, anticoagulant with 3.2% sodium citrate, added amount The ratio of blood sampling is 1:9.   4. Red blood cell fragility test, hematocrit test, blood gas analysis, use a tube covered with a green head, and the anticoagulant is heparin. Electrolyte detection uses lithium heparin as an anticoagulant.   The above are the commonly used test items related to blood, as well as blood collection tubes and additives for blood sampling. Desheng has fifteen years of experience in the field of blood collection reagents. The additives produced include serum separation gel, blood coagulant, blood anticoagulant EDTA salt, heparin, sodium citrate, etc.

ESR (erythrocyte sedimentation rate ESR) refers to the sinking speed of red blood cells under certain conditions. ESR can increase significantly in many pathological conditions. The pathological reasons leading to the marked increase in erythrocyte sedimentation rate are mainly found in various inflammations, tissue damage and necrosis, malignant tumors, hyperglobulinemia and anemia. ESR is often used clinically to observe the activity and dynamic changes of tuberculosis and rheumatic fever.   Because most of them need to test the blood routine at the same time, the blood collection volume is relatively large, especially for children. The author uses EDTA-2K+ anticoagulant to determine and evaluate the erythrocyte sedimentation rate, and make related experiments. The experimental method is: take about 3.2 mL of the subject's venous blood, take 1.2 mL and put it into an erythrocyte sedimentation tube containing 0.3 mL of 109 mmol/L sodium citrate anticoagulant, gently invert and mix; then take 2.0 mL of venous blood The human blood test tube containing 30ul 15% EDTA-2K anticoagulant is gently inverted and mixed, and then 1.2 mL of it is added to the erythrocyte sedimentation tube containing 0.3 mL of 109 mmol/L sodium citrate anticoagulant. The tube also measures the erythrocyte sedimentation rate. Blood collection tube additive EDTA-K2 The principle of anticoagulation between EDTA and sodium citrate is to form a chelate with calcium ions in the blood to prevent blood from clotting. However, the anticoagulant effect of EDTA is stronger than that of sodium citrate, and the two do not interact with each other. For interference, the principle of using the automatic erythrocyte sedimentation rate analyzer is dynamic erythrocyte sedimentation rate analysis. Sodium citrate anticoagulant blood is added to a special test tube, and the fixed hole inside the instrument is vertically placed. According to the setting, the photoelectric detection part of the instrument will scan each cuvette up and down regularly. According to the different light transmittance of glass tube plasma and whole blood, the instrument automatically records the plasma height each time. Through computer processing, the instrument can report the erythrocyte sedimentation rate of 60 minutes and draw the erythrocyte sedimentation time curve.   The experimental results show that the ratio of anticoagulant to whole blood has a great influence on the erythrocyte sedimentation rate, and a high anticoagulant ratio will cause a low erythrocyte sedimentation rate. The test tube used in the instrument method has a small diameter. In actual work, it is common that the amount of blood collected from the sample is not accurate; and the EDTA-2K+ anticoagulant blood measured by a blood cell analyzer is used for the erythrocyte sedimentation rate determination, and the same charge is maintained by complementing the anticoagulation ratio , To ensure the accuracy of the results. This article proves through experiments that EDTA-2K+anticoagulant blood can be used for instrumental ESR determination, and the results are accurate and reliable.   Desheng specializes in the research and development, production and sales of blood collection tube additives and blood test reagents. In terms of blood collection tube additives, it has formed independent intellectual property rights and professional production research and development capabilities. We have accumulated a wealth of knowledge and experience in the pretreatment of blood samples and have the advantages of professional technical services. Welcome to consult and purchase.

The quality control of clinical samples before analysis and testing is extremely important, but in actual work, inspectors often ignore the impact of specimen placement time on the accuracy of the test results, resulting in many specimens that cannot be sent for inspection soon after collection. The process of specimen transportation and the separate inspection of different batches caused a lot of time delay for some specimens. In addition, many hospitals did not indicate the collection time of the specimen on the specimen submission form, and the specific placement time of the specimen is not known. As an inspector, you must have a certain understanding of the impact of specimens placed for too long on the results of different items.   https://www.vacutaineradditives.com/products.html   Impact on biochemical test results 1. Blood sugar: GLU of blood samples at room temperature degrades by about 5% per hour, mainly due to the glycolysis of glucose and the consumption and absorption of sugar by cells. After the blood is separated, glycolysis provides energy for red blood cells, bacteria decompose glucose, and leukocyte degrading enzymes. These factors will cause the blood glucose concentration to gradually decrease with the prolonged storage time.   2. Electrolytes: With the prolongation of the time of blood isolation, the ATP production barrier and the increase of cell membrane permeability lead to the transfer of ions inside and outside the cell. K+ and Na+ levels increase after 4 hours, and to a greater extent after 8 hours. Fluctuations.   3. Liver function indicators: 4 hours after the blood sample was placed, AST and ALP increased, while TBIL and ALT decreased. The main reason is that AST and other factors in red blood cells can be transferred to the outside of the cell to increase the enzyme activity and accelerate the lysis of red blood cells, while TBIL can be oxidized with the bilirubin, resulting in abnormal cytokine levels.   4. Kidney function indicators: blood Scr is not affected by the time of specimen placement, while blood BUN and β2-microglobulin are significantly reduced. It may be that the lipoprotein particle structure has changed or even cracked during the placement of lipoprotein cholesterol, so BUN The levels of β2-microglobulin and β2-microglobulin decreased as the time of blood isolation increased.   It turns out that timely delivery of specimens is so important for biochemical testing. Therefore, in order to control the quality of the test, it is necessary to complete the test of biochemical indicators within 4 hours of placing the blood specimen to ensure the accuracy of the results. Desheng produces blood collection tube additives. The old brand manufacturers, including blood anticoagulant, coagulant, serum separation gel and other products, can be applied to various biochemical testing projects. It is derived from the trust in product quality and professional services. It has accumulated a lot of long-term Repurchase customers, Desheng is your trusted choice!

When registering in September, students have stepped into the school gate, and the lively September has already arrived. This year's students have spent the longest winter vacation in history due to the impact of the epidemic, so they cannot relax their vigilance when the school starts. Desheng comprehensively analyzes the epidemic data of infectious diseases over the years, domestic and foreign epidemic trends, and meteorological and hydrological data, and hereby remind everyone that in September, we should pay special attention to new coronary pneumonia, dengue fever, hand, foot and mouth disease, and foodborne infectious diseases! And sorted out some preventive measures for these diseases. https://www.vacutaineradditives.com/products.html 1. New coronary pneumonia High-risk places: places where people gather at homes, schools, and workplaces. Transmission route: mainly spread through respiratory droplets, close contact, and indirect spread through contact with contaminated items. Symptoms: The main manifestations are fever, dry cough, and fatigue.   Precaution: (1) After the school starts, the school strictly implements daily management, and grasps the health status of faculty, staff and students daily. (2) Carry out the registration system for entering and leaving the school, keep the school doors closed; strengthen indoor ventilation, keep the classroom clean, and high-frequency contact with the surface of objects in public areas, such as door handles, desks and chairs, lecterns, stair rails, elevator buttons And so on, arrange for someone to clean and disinfect every day. If air conditioning is used, the air supply safety of the air conditioning system should be ensured. (3) Keep a safe distance from others in the dining line. No outsiders are allowed to enter the dormitory. Students do not gather or visit in the dormitory area. (4) Masks must be worn when in close contact with people, in crowded, closed places, or in transportation. (5) When there are suspected symptoms such as fever and dry cough, take the initiative to go to a regular hospital for treatment. During the treatment process, you must wear a mask and avoid taking public transportation.   2. Dengue fever Places with high incidence: homes, construction sites, schools and other places where people gather and where there are many mosquitoes. Transmission route: Aedes spread. Symptoms: persistent fever, headache, muscle aches, rash, flushing of face, neck, chest, etc. Preventive measures: The key to dengue fever is to prevent mosquitoes. In recent times, there have been continuous rains and humid air in many places. Friends of the public should always check all kinds of stagnant water in their homes that may have mosquitoes, clean up dead corners, and turn over pots. When going out, wear long-sleeved trousers or apply repellent, and use mosquito coils and mosquito nets at home to prevent mosquito bites.   3. Hand, Foot and Mouth Disease High-incidence places: kindergartens. Transmission route: Respiratory tract transmission, contact with children, and indirect contact with dirty hands, toys and utensils. Symptoms: fever, maculopapular rash and herpes on the palms, soles, buttocks, and herpes or ulcers on the oral mucosa.   Precaution: (1) Pay attention to household and surrounding environmental hygiene, and pay attention to personal hygiene. Wash your hands frequently, do not drink raw water, and do not eat raw or cold food; caregivers should wash their hands before touching children, changing diapers for young children, or after handling feces; often ventilate the room and dry clothes and quilts frequently. (2) The condition of hand, foot and mouth disease in young children changes rapidly, so they should not deal with it by themselves to avoid delay in treatment. Pay close attention to changes in children's conditions and seek medical attention in time. (3) At present, there is no specific medicine for hand, foot and mouth disease. Children of the appropriate age can voluntarily vaccinate hand, foot and mouth disease at their own expense. The EV71 vaccine is targeted for susceptible children ≥ 6 months of age. The sooner the vaccination is, the better; the vaccination procedure is encouraged to be completed before 12 months , In order to play a protective role as soon as possible.   4. Foodborne diseases High-incidence places: unit canteens, school canteens and other collective dining places Transmission route: food transmission. Popular throughout the year, with high incidence in summer and autumn. Symptoms: Mainly acute gastrointestinal symptoms, such as nausea, vomiting, abdominal pain, diarrhea, and occasionally accompanied by fever.   Precaution: (1) Ensure food safety and wash hands frequently. Choose fresh foods, do not buy foods of unknown origin and abnormal sensory properties, and do not eat foods that have exceeded the shelf life. (2) The key is to separate raw and cooked, and cook thoroughly. Generally speaking, fresh food must be fully heated before eating, and seafood and meat must be cooked thoroughly to ensure food safety. (3) When dining out, you should choose a restaurant with a catering service license, and not eat at restaurants with poor sanitation and mobile vendors.

Enzymes come from nature and are natural catalysts for thousands of biochemical reactions that are carried out quickly and efficiently in the body all the time. The natural sources of enzymes include animals, plants and microorganisms. At present, the application of enzyme preparations in detection has become increasingly widespread. Today, Desheng will lead you to understand what is an enzyme preparation and what are its classification methods?   What is an enzyme preparation? Enzyme preparations refer to a class of substances with enzyme characteristics extracted from organisms. Enzyme preparations are proteins composed of amino acids produced by living organisms and can control many reaction processes and biological activities of plants, animals, humans and microorganisms. It is highly efficient, specific, and active under suitable conditions (pH and temperature). Classification method of enzyme preparation: 1. Classification by source (1) Animal's own enzymes: produced and secreted by various secretory glands of animals, almost including all kinds of enzymes required by animals. (2) Artificial enzymes: The artificially extracted enzymes are mainly digestive enzymes.   2. Classified by mechanism of action (1) Hydrolysis enzyme Such enzymes mainly include amylase, protease, lipase, cellulase, phytase, pectinase and so on. (2) Oxidoreductase Oxidoreductase refers to enzymes that participate in the redox of organic substances. There are mainly dehydrogenases and cytochrome oxidases, which are found in the body fluids and tissues of animals and plants, and are not used much in feed additives.   3. Classification according to whether animals can be synthesized (1) Digestive enzymes: livestock and poultry can synthesize these enzymes and digest nutrients, but they need to be strengthened and supplemented for some reason. Such enzymes mainly include amylase, protease and lipase. (2) Non-digestive enzymes: most of the enzymes that animals cannot secrete into the digestive tract are derived from microorganisms. Such enzymes can digest substances that the animals cannot digest or degrade some anti-nutritional factors.   4 Classification by preparation type (1) Single enzyme preparations: such as amylase, lipase, protease, cellulase and phytase. (2) Compound enzyme preparation: It is composed of one or several single enzyme preparations as the main body and mixed with other single enzyme preparations, or obtained by fermentation of one or several microorganisms. The compound enzyme can simultaneously degrade a variety of substrates that need to be degraded in the diet, that is, a variety of anti-nutritional factors and a variety of nutrients, which can maximize the nutritional value of the feed. Feed enzyme preparation products at home and abroad are mainly compound enzyme preparations.   Enzyme preparation is an indispensable additive in our human production and life, and it has a great role in promoting human development. The enzyme preparation products provided by Desheng are mainly used as the test materials of the kit, including α-glucosidase, cholesterol esterase, uricase, coenzyme A, cholesterol oxidase, purine nucleoside phosphorylase, glucose oxidase, etc.

The new coronavirus is a new strain of coronavirus discovered in the human body in 2019. There was an outbreak across the country in early 2020. At that time, experts said that the new crown virus was afraid of high temperatures, and gradually came under control as the temperature rose. Otherwise, under the scorching sun in August, the new crown did not contain its "minions" and continued to rush like people.   On August 17, at the launching ceremony of the National Air Purification Product Quality Supervision and Inspection Center, Academician Zhong Nanshan, the director of the expert committee, attended the event and delivered a speech. At the meeting, Zhong Nanshan responded to topics of public concern such as the recent new local cases and imported frozen food. Regarding the recent new local cases in Shanwei, Guangdong, Zhong Nanshan believes that Guangdong is very tight in prevention and control, and hopes to be controlled. Academician Zhong Nanshan: "Up to now, as far as I know, hundreds of thousands of people have been tested in Shanwei and Shenzhen, followed up with close contacts, and cleaned and disinfected the area. It seems that no new cases have been found. There is hope (to be) controlled the local epidemic."   In response to the public concern about the positive detection of new coronavirus nucleic acid on the imported frozen fresh food packaging, Zhong Nanshan suggested that the public should pay more attention to the contact between frozen meat and fish and wash their hands frequently. "Imported food is relatively rare, so don't overstate it."   "Wear a mask and keep your distance. After the new outbreak, we have put more emphasis on hand washing. Contaminated food, especially frozen meat and fish, should be paid special attention." Recently, sporadic cases still exist everywhere. Since most of the population is still susceptible, Academician Zhong Nanshan pointed out that the prevention and control of the epidemic cannot be let down.   "Every one of us is susceptible, and our antibody detection rate in Guangdong and Guangzhou is only a few tenths of a percent, or even a few tenths of a percent. That is to say, only zero percent. Few people have antibodies, and other people are not immune. As long as you are infected with the virus, you will get it. It turns out that we were too naive, thinking that COVID-19 is similar to SARS, and it should be better in summer (less infectious) Now it seems that summer is still like this."   The prevention and control of the epidemic is not relaxed, for the safety and health of the people across the country. Zhong Nanshan said: “We are always very vigilant about the recurrence of the epidemic anywhere. In the past six months, I have deeply felt that the philosophy of epidemic management at home and abroad is really very different. Some countries are economically first, because if The economy is not good, and some people have no food to eat. But in China, life is first. My experience of this is very profound."

Before answering this question, Desheng will share with you a basic knowledge of microorganisms: the dominant infection rate, that is, how many people will show symptoms after being exposed to pathogens. Correspondingly, there is a recessive infection, that is, after a person is exposed to the pathogen, he is fine, and he may be immunized once because of this, but at the same time, it is also possible that his own immune system cannot defeat it, causing no obvious symptoms, but the virus has always been Low-level replication, and continuous detoxification.   Now that the new crown virus is still spreading, someone asked if someone was infected with new crown pneumonia without knowing it, and then healed on their own? Through the introduction to the basic knowledge of microorganisms at the beginning, I believe you will answer correctly: this situation should exist. Today Desheng will talk to you about this issue.   The core material of nucleic acid detection-virus transport media   From the current point of view, there are two situations after the new coronavirus infects humans. One is the new coronavirus pneumonia infection, which is more common. The other is asymptomatic infection. Academician Zhong Nanshan said in an interview with CCTV News: There are not many asymptomatic infections, but there are. So, what is an asymptomatic infection?   According to the new coronavirus infection pneumonia case monitoring program, asymptomatic infections have no clinical symptoms, but respiratory specimens tested positive for the new coronavirus pathogen. If this asymptomatic infection person has strong immunity, the new coronavirus invades the body, but it is not enough to cause the symptoms of new coronavirus pneumonia such as fever, cough, fatigue, diarrhea, etc.; before long, the new coronavirus is completely affected by the autoimmune system Eliminate and heal itself. Therefore, in theory, this kind of situation may exist in theory that someone is infected without knowing it, and then heals himself.   So, is this asymptomatic infection actually contagious? The pneumonia diagnosis and treatment plan for the new coronavirus infection clearly states that asymptomatic infections may also become the source of infection. At present, many asymptomatic infections have been found. They have no clinical symptoms, but can transmit the virus to others. These asymptomatic infections may be in the incubation period, or they may only carry the virus and will not get sick at all, or they may only have very mild symptoms. They are not aware of it, or even pneumonia will not appear. With the current level of technology, it is difficult Was checked out. Asymptomatic infections who have no symptoms but are contagious are also the most difficult place to prevent and control the current epidemic. Some experts pointed out that although there are asymptomatic infections, but the number is small and the infectious power is not strong, as long as protective measures are taken, there is no need to worry too much.   In this critical stage of epidemic prevention and control, everyone should not forget when the epidemic has improved. It is important to improve immunity. Not going out, not gathering, wearing masks, washing hands frequently, and ventilating are still preventing the new coronavirus, and also responding to it. Effective measures for asymptomatic infected persons. In addition, we also need to cooperate with local policies to actively carry out nucleic acid testing.

Last week, schools in various regions notified schools of corresponding school opening time notifications. Taking Xiangyang City as an example, Xiangyang Education Bureau issued the first autumn school opening notice. The city’s general high schools, secondary vocational schools (including technical schools), junior high schools, and elementary schools are scheduled to start on September 1. ; The kindergarten is scheduled to start on September 7; each college will determine the specific time for the start of school based on actual conditions.   In order to do a good job in the investigation of health risks and to usher in the fall of school with confidence, 77,000 faculty and staff in Xiangyang city began to undergo nucleic acid testing on August 23. Hairong Elementary School is one of the schools designated for testing in the urban area. On August 23, a total of more than 700 faculty members were tested for nucleic acid. Wang Juanjuan, director of the Moral Education Department of Hairong Primary School, said that starting from the 24th, we will enter the centralized training stage and actively prepare children’s education on epidemic prevention knowledge and life safety education.   After completing the nucleic acid test, the city’s faculty and staff are required not to leave Xiangyang unless necessary. Before school starts, the school will carry out the elimination of the campus and the training of teachers on epidemic prevention. City Hairong Primary School teacher Wang Lihong said that in addition to teachers’ participation in nucleic acid testing, other people who are in close contact with students and parents are also tested. After the start of school, all classes are required to carry out the morning and afternoon inspection system and the epidemic report system. It is also understood that after the start of school, 920,000 students in the city, including kindergartens and colleges, will also implement nucleic acid testing and screening for all employees in batches.   As the school starts in September, all colleges and universities should organize staff and students to conduct nucleic acid tests to ensure a safe learning environment. A large number of nucleic acid detection processes are inseparable from the support of Virus Transport Medias. Currently, the market is generally divided into activated and inactivated types according to the composition and function of Virus Transport Medias.   The inactivated type is an inactivated virus that reduces the risk of secondary infection and has lower requirements for the detection environment. Therefore, the inactivated type is generally used for nucleic acid detection in large groups, and the risk of detection is small. The inactivated type can instantly lyse the pathogen to release the nucleic acid, and the protective agent can prevent the accounting from being degraded; and the activated Virus Transport Media can maintain the activity of the virus in a wide temperature range, keeping the originality of the sample to the greatest extent, and can be used Extraction and detection of viral nucleic acid, cultivation and separation of viruses.   The activated type contains Hanks liquid foundation, gentamicin, fungal antibiotics, BSA (V), cryoprotectant, biological buffer and amino acids. A combination of multiple antibiotics has anti-bacterial and anti-fungal effects; bovine serum albumin (BSA), as a protein stabilizer, can form a protective film on the protein shell of the virus, making it difficult to decompose and ensuring the integrity of the virus; Hanks The neutral environment constructed by the buffer helps increase the survival time of the virus and the stability of infection. The activated Virus Transport Media is usually used for the collection and transportation of clinical influenza, avian influenza (such as H7N9), hand, foot and mouth disease, measles and other virus specimens, as well as mycoplasma, ureaplasma, chlamydia and other specimens.   Desheng can provide the above-mentioned inactivated and activated Virus Transport Medias. The two preservation solutions produced have a high detection rate and can be configured for different needs of customers to provide you with high-quality products.

Chemiluminescence immunodiagnosis technology uses chemiluminescence agents, catalytic enzymes and other substances to label antigens or antibodies. When the labeled antigen or antibody is combined with the corresponding antibodies and antigens, the luminescent substrate is affected by the luminescence agents, catalytic enzymes and other substances. Redox reactions. Compared with traditional biochemical diagnosis, chemiluminescence has the advantages of high automation, good specificity, high accuracy, and wide detection range.   Chemiluminescence is a battleground for IVD giants. The luminescence products of multinational giants entered China from the beginning of the 21st century. Siemens first entered the Chinese market in 2003, followed by bioMérieux, Beckman, Johnson & Johnson, and Abbott. In 2006, Roche's electrochemistry Luminous entered China, and imported brands quickly expanded their territory. Later, multinational companies such as Soling, Sysmex, Tosoh and others have also stepped up their efforts in the Chinese market. https://www.vacutaineradditives.com/ Since the first batch of domestically produced automatic chemiluminescence immunoassay analyzers launched by New Industries and Mike in 2011, domestic brands have joined the competition. At present, the domestic luminescence market has basically formed a "4+4" competition pattern. Four foreign giants such as Roche, Abbott, Siemens, and Beckman account for more than 70% of the market share. The top three hospitals are its main customers, and the new industry of domestic enterprises, Antu Bio , Mike Biology and Mindray Medical have nearly 10% market share.   Objectively speaking, the current domestic brands' chemiluminescence has a certain gap with imported brands in indicators such as accuracy, stability, reagent quality and instrument detection speed, and because of the coexistence of multiple technical routes of chemiluminescence detection, it is difficult for manufacturers to trace the value to the source. The product quality is proved by other methods, and the possibility of domestic brands replacing imported brands on a large scale in the short term is low. In the long run, chemiluminescence is a relatively mature detection method after all. As the quality of domestic products is gradually recognized and accepted by the top three hospitals, the low-value detection items of the laboratory will gradually be replaced. We believe that it will account for the total amount of luminescence detection. More than 30% of infectious diseases are expected to take the lead in realizing import substitution. The test results of domestic Antu Biology and other companies are almost the same as Roche's "gold standard". After five years, the overall domestic share of chemiluminescence is expected to reach more than 30%.   In terms of chemiluminescence raw materials, domestic manufacturers of chemiluminescence reagents are gradually getting rid of the current situation of relying heavily on foreign imports for core raw materials such as enzymes, antigens and antibodies. For example, Antu Bio has achieved more than 70% of raw materials in-house. With the continuous development of research and development, domestic IVD The gaps in the field of diagnostic raw materials will be gradually filled. As one of the manufacturers of R&D chemiluminescent reagents, Desheng will also occupy a place in the field of diagnostic raw materials.

Luminol is a commonly used chemiluminescence reagent. The color of its products is best white, followed by milky white, and then light yellow. Luminol developed and produced by Desheng is milky white powder, which is easily soluble in lye. It is also soluble in dilute acid, almost insoluble in water, and difficult to dissolve in alcohol. It will only show luminescence when it is used as a solution. At the same time, when we install this product, we need to use a light-shielding and well-closed container. The temperature should be guaranteed to be 0-5°C, and attention should be paid to avoid light and moisture, so as to ensure that the product inspection quality is more accurate and higher. Luminol has a wide range of uses. In addition to criminal investigation, it can also be used for immunochemiluminescence reactions, for the detection of sex hormones, drug abuse, and cardiovascular and reproductive diseases. In view of its application value and broad market prospects, the advantages of our company's patented preparation method compared with traditional preparation methods are worthy of attention. Traditional preparation method:   (1) Using 3-nitrophthalic acid as the raw material, the cyclization reaction with hydrazine hydrate is carried out, and luminol is obtained by reduction with sodium hydroxide. The synthetic method has a simple process route, but the disadvantages are: 1. The first step is high reaction temperature and requires 225°C; 2. The purification is difficult. The first step requires high boiling point triethylene glycol as a solvent, which is difficult to remove. The reducing agent used in the second step will decompose and produce several inorganic impurities during the reaction process, which are difficult to remove; 3. The yield is low, only about 30%.   (2) Using 3-nitrophthalic acid as a raw material, the cyclization reaction is carried out with hydrazine sulfate, and luminol is obtained by reduction with sodium hydroxide. The synthesis method has been improved on Route 1, but the disadvantages are: 1. The highly toxic hydrazine sulfate is used; 2. The reaction temperature in the first step is 170 degrees, which is too high and requires high equipment; 3. The reaction produces a lot of In the waste liquid, the reducing agent used in the second step will decompose and produce several inorganic impurities during the reaction, which are difficult to remove.   (3) 3-Phthalic anhydride is used as raw material, nitrated with mixed acid to obtain 3-nitrophthalic acid, dehydrated with acetic anhydride to obtain 3-nitrophthalic anhydride, then hydrazinolysis, and finally iron powder reduction Get luminol. The shortcomings of the synthesis method are: 1. The synthesis route is longer; 2. The mixed acid nitrification produces a large amount of acidic waste liquid; 3. The reduction of iron powder results in a large amount of iron slag waste, which causes great environmental pollution.   Desheng preparation method: The method of "a one-pot synthesis of luminol or isoluminol" developed by Desheng has obtained a patent. Compared with the prior art, its advantages and beneficial effects are obvious.   1) This method realizes that the three-step reaction is completed in the same pot, the intermediate product does not need any purification treatment, and the final product is directly obtained.   2) The method has simple synthesis route, mild reaction conditions, simple operation, and all the reagents required are conventional reagents, and the equipment required is conventional equipment, and the price is low. Therefore, the cost required for synthesis is low, and it is suitable for large-scale industrial production. .   3) The yield and purity of luminol and isoluminol synthesized by this method are high, the yields of luminol and isoluminol are all above 80%, and the HPLC (high performance liquid chromatography) purity is above 98% , Can fully meet the needs of industrialized production of products and market demand.

Routine blood testing is already a very important routine testing item. Platelet count is an important basis for the diagnosis and identification of hemostasis and thrombotic diseases. However, in daily testing, the platelet count using a blood cell counter may sometimes be falsely increased or Lower, why is this?   The role of platelets Platelet is abbreviated as PLT, which belongs to blood cell like red blood cell and white blood cell. It is dimpled, oval or disk-shaped on both sides, but much smaller than red blood cell and white blood cell. The average diameter is only 2 to 4 microns. It is formed by fragments of bone marrow megakaryocyte cytoplasm shedding. . When blood vessels are damaged, platelets aggregate into clusters to form thrombus; and also release substances that promote vasoconstriction and blood clotting. Platelet count refers to the number of platelets contained in a unit volume of blood. Certain diseases can lead to a decrease or increase in the number of platelets. Causes of false elevation of platelets 1. In some hemolytic diseases, hemolysis occurs in the blood vessels, and then the fragments generated by the rupture of red blood cells will be divided into the platelet area by the hemocytometer, which will cause errors in the instrument and falsely increase the count. For example, hemoglobinopathy and G-6-PD deficiency can cause intravascular hemolysis.   2. After the treatment of chronic granulocyte patients, a large number of fragments will appear in the blood vessels. These fragments will also be divided into the platelet area by the hemocytometer, which will cause interference and falsely increase the platelet count.   3. Take venous blood for routine blood testing within a short period of time after transfusion of fat emulsion, chylo particles will also be counted into platelets by the blood cell counter, resulting in high falsehood. All the above three types can be corrected by observing the morphology of platelets and red and white blood cells through artificial blood smears.   Causes of false reduction in platelets 1. EDTA-K2 anticoagulant causes platelet aggregation, which is related to the presence of cryptic antigens on the surface of platelets, causing the blood cell counter to treat the adhered platelets as non-platelets, resulting in a false decrease in counts. At this time, citrate can be used instead of EDTA-K2 as an anticoagulant, or platelets can be manually counted to correct.   2. Cold agglutinin can agglutinate red blood cells, nucleated cells, and platelets, thereby making the platelet count falsely lower. When encountering the interference of cold agglutinin in the body, the specimen can be incubated at 37 degrees for a period of time to eliminate the interference caused by cold agglutinin.   3. The invisible aggregation of platelets in hypercoagulable blood will make the count falsely lower. For example, hypermagnesium, hypercholesterolemia, and high triglycerides will all increase platelet aggregation.   The instrument used for blood cell analysis has the advantages of speed and simplicity, but it is also easy to cause errors. As an established blood test reagent company, Desheng recommends that when the platelet count does not match the clinical practice, it must be corrected by manual recheck to avoid unnecessary disputes between doctors and patients.

As the weather becomes hot, all chemical companies are in great danger. It is necessary to strictly pay attention to the storage conditions of raw materials and the temperature control during equipment operation to avoid safety accidents. According to the recent notice issued by the Jiangsu Provincial Emergency Management Department, chemical (hazardous chemicals) companies are required to implement eight major risk management and control measures, and do a good job in safe production of hazardous chemicals in summer and flood seasons, and make every effort to prevent and contain production safety accidents. https://www.vacutaineradditives.com/ The eight control measures are as follows: One is to earnestly implement monitoring measures for major hazard sources, strengthen the temperature and pressure monitoring of hazardous chemical production equipment and storage facilities, prohibit over-temperature, over-pressure, and overload production, pay close attention to the working conditions of heat exchange equipment and cooling water, and handle temperature in a timely manner , Liquid level, pressure and other abnormal conditions.   The second is to strengthen the maintenance of equipment and facilities. All automatic control and safety instrument systems, flammable and toxic gas detection and alarm systems, etc. should be put into normal operation, and buildings (structures), production equipment, storage facilities, electrical equipment, safety instruments, etc. Lightning protection and grounding conditions completely eliminate hidden dangers and effectively prevent production safety accidents caused by lightning.   The third is to improve ventilation and cooling facilities. In places involving the production, storage, and use of flammable, explosive, toxic, harmful and other hazardous chemicals, it is necessary to ensure that the ventilation, cooling, detection and alarm facilities are intact and in a normal applicable state.   The fourth is to reasonably adjust the work and rest time, arrange the time reasonably according to the weather forecast and weather changes, reduce outdoor outdoor work activities at noon high temperature, and equip heatstroke prevention and cooling drugs to prevent personnel from heatstroke. Enterprises should strictly implement the safety management regulations for the loading and unloading of hazardous chemicals in summer. When the temperature exceeds 35°C, all hazardous chemical loading and unloading operations shall be stopped from 10 am to 15 pm.   The fifth is to strengthen the safety management of inspection and maintenance, fully identify the risks of special operations such as hot work, operations in restricted spaces, temporary electricity use, and climbing operations, conduct inspection and analysis, standardize the approval of work permits, and effectively confirm the implementation of safety measures.   The sixth is to do a good job in flood prevention. Enterprises should inspect the drainage facilities in the plant area, dredge drainage, flood drainage ditches, arrange flood control materials, and implement flood control rescue teams. It is necessary to clarify the responsible personnel and regularly monitor the flammable and toxic gases in the sewage pipe network to prevent leaked materials from entering the sewage pipe network and ensure the safe operation of the pipe network. Enterprises along the river, lake, and river should pay close attention to flood forecasts, inspect flood discharge isolation gate facilities such as the river, lake, and river, arrange flood control personnel on duty, and be ready to close the gate at any time to prevent rivers, lakes, and rivers from flowing. Backflow.   The seventh is to inspect and test the fire sprinkler system, and conduct a start-up test on the sprinkler cooling water system installed in the hazardous chemical tank area to ensure that the water pressure and cooling effect meet the design requirements.   The eighth is to pay attention to the safety of enterprises that stop production, and urge enterprises to fully understand the situation of hazardous chemicals in storage tanks and installations, and properly handle, recover and transfer hazardous materials. Strengthen the monitoring and control of out-of-service production installations and storage facilities that contain hazardous chemicals, and shall not dismantle or disable safety facilities without authorization.   Desheng specializes in the research and development, production and sales of blood collection tube additives, in vitro diagnostic reagents, buffers, and luminescent substrates. The storage of raw materials, equipment monitoring, and mental state supervision of production personnel are all strictly supervised to avoid safety accidents. In the scorching summer, Desheng hopes that all chemical companies will attach great importance to safe production.