China Wuhan Desheng Biochemical Technology Co., Ltd Company News

3-(cyclohexylamine)-1-propanesulfonic acid, or CAPS, is a Good Buffer Solution, commonly used in biochemical diagnostic kits, DNA/RNA extraction kits, and PCR diagnostic kits. The buffer used in enzyme chemistry and HPLC separation of basic drugs has relatively stable properties. The pKa value at 25°C is 10.4 and the pH buffer range is 9.7-11.1. It is widely used in biochemical analysis and in vitro diagnostic industries.   Raw materials for making CAP   In addition to the biochemical industry, the application fields of CAPS are also very extensive: 1. CAPS is widely used as a biological buffer: used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits; buffers for enzyme chemistry and HPLC separation of basic drugs. When CAPS is used as a biological buffer, it needs better purity. Therefore, it is generally necessary to analyze purity. When it is used in industry, the purity requirement is relatively low. It needs to be treated differently for different applications.   2. CAPS in industry: used in new coatings and new materials. CAPS is also a raw material for manufacturing welding materials, air-conditioning equipment and manufacturing lithium metal.   3. Used as analytical reagent, heat exchange carrier, and also used in pharmaceutical industry. It is used for air conditioning and also for making fireworks; dry batteries and lithium metal are also used as flux and desiccant.   4. For the coating industry, it is used as a water-based isohydrogen ester curing agent. Under normal temperature, the water-based isocyanate curing agent can coexist with the resin containing active groups (hydroxyl, carboxyl, amine, epoxy, etc.) for a long time.   Because CAPS is very versatile, and there are many manufacturers, we must identify large manufacturers with production qualifications when selecting manufacturers, and pay attention to avoiding middlemen to make profits. Hubei New Desheng Material Technology Co., Ltd. is located in C8-2, Guanggu United Technology City, Gedian Development Zone, Ezhou City, Hubei Province. It has 14 years of R&D and production experience in the biochemical field. The company specializes in the production of Chemiluminescent substrates and other in vitro diagnostic reagents, and the company has passed ISO-9001 quality management system certification, has a good reputation in the industry, is a trusted bio-chemical raw material production enterprise, choose Desheng is definitely a good decision!

DNA nucleic acid electrophoresis is an important step in nucleic acid PCR, separation and purification, nucleic acid detection and other tests. The agarose gel electrophoresis kit is an electrophoresis kit product developed to facilitate nucleic acid electrophoresis. Compared with traditional gel electrophoresis has many advantages. Agarose gel, electrophoresis fluid and loading buffer   Electrophoresis refers to the movement of charged particles toward an electrode opposite to their electrical properties under the action of an electric field. Under certain conditions, the moving rate (mobility) of charged particles is fixed. In DNA electrophoresis or Southern Blot blot hybridization, charged particles refer to nucleic acid DNA, and different DNAs have different mobilities and thus separate from each other. Since nucleic acid particles are very sensitive to pH, a buffer must be added to the electrophoresis solution to nucleic acids. The buffer components are contained in agarose gel, TAE or TBE electrophoresis solution, loading buffer.   Generally, agarose gel electrophoresis needs to go through the following steps: preparation of agarose electrophoresis gel, preparation of electrophoresis solution, spotting, electrophoresis, and cleaning of electrophoresis tank. Among them, the longest time is the preparation of agarose gel. It needs to prepare a mixing solution, weigh agarose, melt in a microwave oven, cool the solution to 60 degrees, pour the gel to cool, and clean the equipment. The process is very cumbersome, and repeated in large quantities, it takes 1 ~ 1.5 hours. The agarose gel electrophoresis kit is different: 1. There is no need to purchase reagents such as agarose, nucleic acid dye, electrophoresis solution and loading buffer. 2. Eliminate the cumbersomeness of making gel, and the pre-made gel is pre-stained with nucleic acid dyes, no need for electrophoresis solution staining or post-staining. Simple and convenient, ready to use. No need to add nucleic acid dye in DNA samples or electrophoresis solution when using pre-made gel, reducing the waste of nucleic acid dyes, and the extremely low toxicity of pre-stained gels is much safer for operators than directly use nucleic acid dyes. 3. The kit is specially equipped with high-pressure fast electrophoresis solution. If your nucleic acid sample fragment is below 2000bp, you can control the speed of electrophoresis at any time and adjust the voltage. The general mini gel can be completed in 5-10 minutes at the fastest; Marker or Nucleic acid samples have many bands and long fragments (nucleic acid sample fragments are larger than 2000bp), which can be adjusted to a suitable low voltage, or you can still use your original low-voltage electrophoresis conditions. 4. The electrophoresis of this product does not affect the subsequent Southern hybridization, and the obtained DNA fragments are subjected to gel recovery, and the subsequent DNA ligation and other reactions are not affected.   In short, compared with the traditional nucleic acid electrophoresis method, the agarose precast gel electrophoresis kit has the advantages of saving time,saving labor , high pressure, fast, and saving cost . It is a product strongly recommended by Desheng. Of course, there are TAE, TBE electrophoresis kits or Tris buffer or other buffers can also contact our company.

Virus Transport Media is divided into inactivated type and activated type. It is a solution that protects the head of the virus swab after sampling in the virus sampling tube, which can prevent the swab from being immediately after the virus sampling In the case of detection, it can also be stored or transported for a period of time to prevent the viral nucleic acid from being decomposed and undetectable.   There are many steps to detect the entire viral nucleic acid. Among them, sampling with nasopharyngeal swabs or other swabs, as well as the storage and transportation of viral samples are the pre-processing steps of nucleic acid detection. Swabs are a more commonly used method of taking biological samples, and can be used for molecular biology analysis such as PCR and nucleic acid detection. The characteristics of swab sampling are fast and non-intrusive, which will not cause harm or other impact to the sampled object. It is very suitable for large-scale screening sampling and sampling of special groups (such as children and the elderly) and tissues and organs.   Since most virus sampling sites do not have the conditions for immediate detection, it is important to store and transport the virus for inspection, and the virus is difficult to survive in vitro, so it is necessary to use the virus transport media The virus sample soaked up. Among them, the inactivated virus transport media is safer, and conventional cryopreservation is sufficient. The samples stored in the activated virus transport media have shorter storage time or require strict cryopreservation, but the detection rate is higher, and not only can be used for nucleic acids Detection. However, it should be noted that the more virus transport media in the sampling tube, the better the preservation. Because the virus transport media is a solution, it will have a dilution effect on the virus sample. Too much addition will reduce the detection rate of nucleic acid detection.   After the virus sample is delivered to the testing institution, the nucleic acid is purified through the processes of lysate lysis, centrifugation, separation, etc. When extracting DNA or RNA, care should be taken to prevent the cleavage of the nucleic acid. RNA samples also need to undergo reverse transcription reactions through reverse transcription primers, dNTPs, reverse transcriptase, etc. to produce the corresponding DNA. Then, the DNA polymerase catalyzes PCR amplification with specific primers of viral cDNA. If there are amplified DNA bands, it can be determined that the sample contains virus, otherwise it is not.   Virus transport media, sampling swabs, and sampling tubes related to virus detection are the products recommended by Desheng. Especially in the case of serious epidemics, it is also the company's purpose to provide high-quality goods for related products in short supply in the market. Large quantities of preservatives and swabs can also be discounted.

Due to the New coronavirus epidemic, Our work and life have been greatly affected. The detection of biological viruses and nucleic acids has also become well known from the public, and the corresponding virus sampling and virus transport media has also become a kind of biological reagent raw material with great demand in the market. There is a huge demand for a raw material of biological reagents. Of course, many people are curious about how it is made.   According to the different functions of virus transport media, there are two types of inactivated and activated types, of course, the production method is also different. This article focuses on the inactivated virus transport media. The biggest difference between the inactivated and activated types is that the inactivated type does not need to maintain the integrity of the virus structure, only need to release its nucleic acid, and then can be detected by nucleic acid detection steps such as NT-PCR and probe testing of nucleic acids If the virus sample has characteristic nucleic acid, it can be judged whether the virus test of the sampling object is positive or infected. Biological virus is a kind of microorganism with simple structure composed of nucleic acid DNA or RNA plus protein. If the sample contains virus characteristic nucleic acid, it can be judged that the sample is infected by the virus.   Desheng Physical and Chemical Performance Testing Laboratory   Since the virus is inactivated without culturing the virus, the first thing that is needed is to cleave and inactivate the virus and destroy its membrane protein to release nucleic acid. Usually, the prepared virus transport media is added with a cracking salt. The cracking salts used by different companies may be different, including guanidine hydrochloride, guanidine isothiocyanate, etc. The essential role is the same, which is to split the viral membrane protein and extract the encapsulated viral nucleic acid. When sampling, the nucleic acid cannot usually be detected immediately. The released nucleic acid will be degraded by contact with RNase in the air. Therefore, an RNase inhibitor needs to be added to the storage solution to inhibit its catalytic RNA degradation. In addition, you need to add Tris buffer and EDTA to maintain the pH of the environment where the nucleic acid is located. Use the characteristics of EDTA to complex metal ions such as calcium, magnesium, iron, etc., to prevent metal ions from activating proteases, reduce the impact on nucleic acid quality, and improve nucleic acid. Stability, extend the storage time.   Use deionized water when configuring the virus transport media, or use ultrapure water for special test requirements. The configuration is similar to our usual configuration of biological buffer, but the temperature requirements are stricter, and the temperature must be kept low during storage and transportation. The virus transport media involves virus sampling and nucleic acid detection, and it must be treated with rigour. After configuration, it needs to be tested for virus inactivation and positive sample detection rate.   The preparation of the virus transport media seems simple, but it is not sloppy for the actual production. It must ensure that the virus is inactivated and loses its infectivity, and it must inhibit the degradation of nucleic acid by RNase. Any failure to do a good job will affect the final detection. Desheng Technology organized scientific researchers to consult materials, consult experts, repeat experiments, and verify by multiple parties, and finally successfully developed a virus transport meida for the new coronavirus.

  HEPES, 4-hydroxyethyl piperazine ethanesulfonic acid, utilizes the reducibility of HEPES to excess metals at specific pH values, and uses HEPES-NaOH reduction method to prepare gold nanoparticles. This method is simple to operate, fast to react, easy to control, less by-products, and environmentally friendly.   Preparation of gold nanoparticles   1. Prepare chloroauric acid solution with concentration of 0.05-10 mmol/L;   2. Prepare HEPES buffer with concentration of 5-50 mmol/L, and adjust the PH value of HEPES buffer to 7.0-8.0 with sodium hydroxide;   3. Add surfactant to HEPES buffer prepared in step2 to prepare surfactant solution with a concentration of 1-2 mmol/L;   4. The surfactant solution prepared in step3 is introduced into the reaction tank, and the chloroauric acid solution prepared in step1 is slowly added to the reaction tank according to the molar ratio of chloroauric acid solution and surfactant solution of 1:1-1:10, and stirred at a speed of 200-300r/min. The reaction lasts for 5-30 min to obtain a mixture containing nanogold colloids;   5. The mixture prepared in step4 is dried and purified to obtain nanogold particles.   Taking the HEPES buffer with a concentration of 50 mmol/L as an example, the configuration method is as follows: firstly, 2.38 kg of HEPES is weighed and dissolved in 180 L of deionized water, and then the pH value of the solution is about 5.4 by using a pH meter, then 0.1 mol/L of sodium hydroxide solution is added slowly and stirred continuously until the pH is adjusted to 7.4; finally, deionized water is added to 200L.   Preparation of HEPES   Method 1   Using 1,2-dichloroethane as solvent, hydroxyethyl piperazine (5.00g, 0.02mol), potassium carbonate K2CO3 (6.00g, 0.04mol), 50mL 1,2-dichloroethane were added into a 100mL three-port bottle equipped with mechanical stirring and thermometer. The oil bath was heated at 90℃ (1,2-dichloroethane boiling point 85℃), and the reaction was stirred for 20h.Stop the reaction, filter and wash the filtered salt with 200 mL ethyl acetate (EA).The filtrate was dried to obtain 2.6 g HEPES solid.   Method 2   11.0g (84.5mmol) anhydrous sodium sulfite, 27.0 mL(343.6mmol) dichloroethane, 120mL water, 110mL ethanol, 50mg copper powder were added into three bottles with magnetic stirrer and reflux condensation tube in turn. The oil bath was heated and heated to reflux.After reflux for 22h, the reaction liquid was evaporated under reduced pressure to remove water until all white solids were precipitated.This solid is mainly composed of products, unreacted raw materials and salt produced.The obtained solid and 500mL ethanol were added into a 1L flask and heated for reflux for 40min.Drain and filter while hot, and let the filtrate cool down, then leave it at 0℃ overnight.Drainage filtration and vacuum drying resulted in flake crystallization with yield of 11.40 g and yield of 81.0%.   Sodium chloroethyl sulfonate (15.10 g, 0.08 mol), hydroxyethyl piperazine (9.88 g, 0.075 mol), 60 mL water and oil bath were added into four flasks with magnetic stirrer, reflux condensation tube and thermometer to stir the reaction at 105℃. As the reaction proceeded, the pH of the reaction solution would decrease, and 5 mol/LNaOH aqueous solution was added dropwise to control the pH at about 9. A total of 15 mL was added and the reaction continued for 5h.   At the end of the reaction, the reaction solution was diluted to 500mL with water, and the upper ion exchange resin column (about 500g) was desalted and purified. After the reaction solution was all loaded on the column, it was washed with distilled water until the effluent pH was 6, and then washed with 1mol/L ammonia water. The effluent with product points (pH about 5-9) was collected for TLC detection.Rotary evaporation was concentrated to 150 mL, activated carbon decolorization was added, oil bath was 110℃, heating and stirring for 0.5h, filtration, rotary drying of filtrate, adding 50mL ethanol, heating reflux for 0.5 h, thermal filtration, the white solid obtained after drying was 8.63G.The filtrate was dripped with glacial acetic acid, adjusted to pH 5, cooled overnight at 0℃, and filtered. The solid obtained was 2.80G after drying.A total of 11.43g of HEPES solid was obtained in 64.5% yield.   The preparation method of 10mmol/L HEPES buffer is as follows: weigh 2.383g of HEPES accurately, add fresh three-steamed water to constant volume to 1 L.Filtration sterilization, storage at 4℃ after sub-packaging.If used as a buffer when added to the cell culture medium, it is recommended that the culture medium be kept away from light.   Hubei New Desheng is a manufacturer of biochemical raw materials with 14 years of R&D and production experience. It can provide various specifications of biological buffers (HEPES, Tris, BICINE, CAPS, TAPS, etc.), chemiluminescent reagents, blood collection additives, chromogen substrates, enzyme preparations, antigen antibodies, etc. Welcome to call for detailed inquiries!

With the development of the times, people pay more attention to the quality of life, home is a warm and comfortable place for everyone to enjoy, but many decoration companies in order to save costs in the choice of paint is not satisfactory.Therefore, in response to increasingly stringent environmental regulations, water-dispersible polyisocyanates have shown importance in various applications in recent years.At present, water-dispersible polyisocyanates in most applications are non-ionic hydrophilic modification by polyethers.Although this hydrophilic modified polyisocyanate has gained wide market recognition in most applications, it also has certain drawbacks, such as its high viscosity, which requires a considerable shear force to be applied in the construction process to uniformly introduce it into the aqueous medium.In order to avoid these shortcomings, this also gives CAPS an excellent opportunity.Let it find its location in new coatings.   CAPS in Packaging   Ion-modified groups include carboxyl group, sulfuric acid group, hydroxyl group, hydroxy sulfonic acid, etc., but there are still some defects, such as carboxyl-modified polymers are prone to gelation, hydroxy sulfonic acid-modified products are obviously yellow in color, etc.Later, Bayer reported 3-(cyclohexylamino)-propanesulfonic acid (CAPS)-modified polyisocyanate in its patent CN1429240A. The study found that CAPS-modified polyisocyanate could be finely dispersed in water and the product was stable in storage.CAPS-modified polyisocyanate exhibits certain advantages over other ionic or non-ionic modified products.   1. 3-(cyclohexylamino)-1-propane sulfonic acid (CAPS) reacts with aliphatic polyisocyanates (the former is a zwitterionic aminosulfonate) under mild conditions and in the presence of tertiary amine neutralizers, and the resulting urea sulfonate derivatives are excellent emulsifiers.Regardless of salt forming groups, CAPS-modified polyisocyanates have good storage stability and are not turbid. Even if they contain fewer sulfonate groups, they can get well dispersed emulsions in water.A series of ionized modified polyisocyanates can be obtained for use in various environmentally friendly high quality waterborne two-component polyurethane coatings.These coatings are comparable to general solvent-based coatings in terms of dry, curing and chemical resistance.The new regulations require further reduction of VOC (volatile organic compounds), and the use of these crosslinkers will definitely increase in the future. Compared with solvent-based coatings, they will not lead to a decrease in paint film quality.   2. Closed water-dispersible polyisocyanate curing agent: Closed water-dispersible polyisocyanate curing agent is a kind of closed polyisocyanate curing agent that is hydrophilically modified so that such products can be dispersed in aqueous resin system.Under the condition of high temperature baking, the blocking agent will be unblocked from the system, releasing isocyanate groups, which react with hydroxyl groups.   3. Closed water dispersible polyisocyanate curing agent is mainly used as crosslinking agent in high temperature baking system.At present, the main use is in the Mid-coating of automobile original paint, in addition, there are also some applications in water-based industrial paint.Closed water dispersible polyisocyanate curing agent can also be used with melamine curing agent to reduce costs,and closed water dispersible polyisocyanate curing agent to improve performance.   CAPS is used as a biological buffer in addition to new materials and coatings, in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits, and in buffer solutions for enzymatic chemistry and HPLC separation of alkaline drugs.  

Trimethylolaminomethane, CAS77-86-1, commonly known as Tris, also known as tromethamine, is a very commonly used reagent, which is widely used in industrial synthesis, biochemical testing, and biopharmaceutical fields; The virus transport media sample tube belongs to an important raw material of its configuration solution.   Trismethylaminomethane Tris molecular structure contains one amino group and three alcoholic hydroxyl groups. The amino group and three methylol groups form a methane-like tetrahedral structure around the central carbon atom. Due to the presence of amino groups, Tris is weakly basic in aqueous solution. The amino group can be used as a coordination group to form Tris salts with many acids. Commonly, Tris-HCl, TEA, TEB, Tris glycine, and Tris phosphoric acid are also used. The raw materials used in the virus transport media are Tris and EDTA. The actual TE buffer is Tris plus EDTA.   Tris powder in drum   Adding Tris to the virus transport meida can first maintain the pH value of the sampled sample and act as a biological buffer. The virus and its nucleic acid are relatively sensitive to the environmental pH. The nucleic acid (DNA, RNA) is easily hydrolyzed in acidic solution. It is more stable in neutral or weak alkaline solution. The Tris hydroxymethylaminomethane, PH buffer range: 7.0-9.0, can maintain the stability of the nucleic acid released after the sample to be cleaved to avoid degradation of the nucleic acid, increase the concentration and purity of the nucleic acid, and help to improve the quality of the nucleic acid To ensure the accuracy of subsequent nucleic acid detection and analysis operations.   Tris buffer is a weak alkaline solution. In such a solution, DNA will be deprotonated to improve its solubility. Therefore, Tris buffer is generally used in the dissolution of nucleic acids and nucleic acid extraction. It should be noted that because it is alkaline when disposing the solution, it will absorb carbon dioxide gas that can generate carbon dioxide in part of the air, so the cap of the bottle containing the Tris solution needs to be tightly capped. In addition, the Tris solution is sensitive to temperature. For every increase of 1 degree, the pH value drops by 0.03, and it needs to be maintained at room temperature during configuration.   Tris buffer is more and more widely used, and even has a tendency to exceed phosphate buffer. Because it does not react with calcium, magnesium and heavy metal ions, its performance is superior to phosphate buffer in many aspects. Desheng's products related to Tris include Tris reagent, Tris hydrochloric acid, TEA/TEB electrophoresis electrophoresis gel, etc., which are superior in quality and low in price.  

Due to the impact of the epidemic, the topic of novel coronavirus has become our daily topic. Recently, Wuhan has implemented the national nucleic acid test. When it comes to nucleic acid detection, we will talk about the virus transport media developed and produced by Desheng. What role does it play in nucleic acid detection?               At present, there are two types of virus transport media: inactivated and activated type.               Virus sampling swab is a common method of virus sampling combined with PCR, which can be used for rapid detection of viral diseases. However, not every sample collection place can carry out PCR detection, so it is necessary to transport the collected virus swab samples, so the swab virus transport media came into being.               Desheng’s virus transport media             For different detection purposes, different virus transport medias need to be used. At present, the two widely used transport media have their own characteristics. In order to meet the different requirements of detection and different laboratory conditions of virus detection, it is necessary to sample different transport media.               Virus transport media (inactivated type) can be used to inactivate and preserve respiratory pathogens quickly by using lysate, which makes the samples lose infectivity. The inactivated samples can be matched with a variety of virus DNA/RNA extraction kits, M32/M96 nucleic acid extractor for rapid extraction of nucleic acid, and the respiratory pathogen PCR detection kit for rapid detection. The specificity sensitivity is not affected.               Virus transport media (activated type) contains Hanks liquid base, gentamicin, fungal antibiotics, BSA (V), cryoprotectants, biological buffers and amino acids. The combination of multiple antibiotics has the effect of anti bacteria and anti fungus; BSA, as a protein stabilizer, can form a protective film on the protein shell of the virus, making it difficult to decompose and ensuring the integrity of the virus; the neutral environment constructed by Hanks buffer helps to increase the survival time and infection stability of the virus. The activated virus transport media is usually used for the collection and transportation of clinical influenza, avian influenza (such as H7N9), hand-foot-mouth disease, measles and mycoplasma, Ureaplasma and chlamydia.               Desheng technology has been committed to the R&D and production of virus transport media, carbomer and other products since the resumption of the epidemic, and has achieved a breakthrough victory. The affirmation of customers after use is a great encouragement to us! We will continue to do our products well, not for the immediate interests, only for better development and customer trust.                      

Virus transport media is a kind of liquid to protect the tested substance of virus by immersing the virus sample on the sampling swab in the sampling tube. It is generally divided into two types: one is activated type, which can protect the protein and nucleic acid of virus; the other is inactivated type, which usually contains the lysate of inactivated virus, which can lyse the protein and protect the nucleic acid.   Therefore, the virus transport media added with lysed salt is an inactivated virus transport media. The main purpose of the contained Tris, lysed salt, EDTA, etc. is to lyse the nucleic acid and release the nucleic acid, so as to carry out by subsequent real-time fluorescent RT-PCR Nucleic acid detection, to determine whether the sample contains viral characteristic nucleic acid, that is, whether it is infected with a virus.   Inactivated and activated virus transport media   Nucleic acid is a biological macromolecular compound composed of many nucleotides, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). It is one of the most basic substances in life. The virus has a single structure and contains only one kind of nucleic acid and protein, so when the characteristic nucleic acid is detected, the virus is detected. Viruses are parasitic organisms and cannot survive in vitro after sampling. If they cannot be detected in time, they need to be put into the virus transport media. In order to protect the security of the virus detection environment, it is necessary to add a lysed salt to inactivate the virus and release the nucleic acid that can be detected.   Guanidine is a nitrogen-containing organic compound, also known as "iminourea", "imicarbazide", and "carbamidine". Cleavage salts are strong inhibitors of nucleases and facilitate the extraction of complete RNA from RNASE-rich tissues. The lysed salt can not only quickly destroy the cell membrane, but also denature the protein, so that the protein is denatured and precipitated, so that the nucleic acid can get rid of the protein. The inactivated virus transport media containing lysed salt can fully and effectively lyse the cell, so that the nucleic acid in the cell can be fully released, and a higher concentration of nucleic acid is obtained, which is conducive to improving the quality of the nucleic acid and ensuring the accuracy of subsequent operations.   In addition to the inactivated virus transport media, Desheng developed and produced a activated virus transport media, which not only saves the integrity of the virus, but also has a higher detection rate. It can also be used for other research besides nucleic acid detection. The inactivated virus transport media is safer to use, the operating environment requirements are not so strict, and each has its own advantages.

Tris (trihydroxymethylaminomethane) is a weak base with a pKa of 8.1 at room temperature of 25℃ and an effective buffer range of pH 7.0 ~ 9.2.The pH of aqueous solution of Tris alkali is about 10.5. Hydrochloric acid is generally added to adjust the pH value to the desired value, so that the buffer of this pH value can be obtained.Since Tris buffer is a weakly alkaline solution, DNA will be deprotonated in such a solution, thus improving its solubility.If the pH-adjusted acid solution is replaced with acetic acid, a "TAE buffer" (Tris/Acetate/EDTA) is obtained, while a "TBE buffer" (Tris/Borate/EDTA) is obtained by replacing it with boric acid.These two buffers are commonly used in nucleic acid electrophoresis experiments.TAE, TBE, etc. prepared by Tris are the most commonly used reagents for DNA electrophoresis, and TE (pH 8.0) is mainly used to dissolve DNA.(TE is a combination of Tris and EDTA.)1MTris-HCl6.8 and 1.5MTris-HCl8.8 are the most commonly used reagents for SDS-PAGE.   TRIS reagent   Among the conventional operations of genetic engineering, agarose gel electrophoresis is the most widely used.Agarose gel electrophoresis is a conventional method for separating, identifying and purifying DNA fragments.It usually uses a horizontal electrophoresis device to electrophoresis under an electric field with constant intensity and direction.DNA molecules are negatively charged in gel buffers (generally alkaline) and migrate from negative to positive electrodes in an electric field.The rate of DNA molecule migration is dependent on the size and conformation of the molecule.The influence of electric field strength and direction, base composition, temperature and embedded dyes, etc.     Operation process: ① TE buffer: (10 mmol/L Tris-HCl, 1 mmol/L EDTA) Electrophoresis buffer (50XTAE)   ② Electrophoresis buffer (50XTAE): Tris 242g, glacial acetic acid 57.1ml, EDTA (0.5mol/L pH 8.0) 100ml Dilute 50 times with distilled water when used.   ③ Sample buffer (6X): 0.25% bromophenol blue, 0.25% xylene blue, 40% (W/V) sucrose   ④ STET buffer (pH 8.0) (8% sucrose, 0.5% Triton, 50 mmol/L EDTA, 10 mmol/L Tris) 1) Measure 100 ml of TAE electrophoresis solution, add 0.7 g of agarose, mix well, place in microwave oven, heat for 3 minutes, fully dissolve agarose. 2) Close the clean and dry electrophoretic plate with sterilized rubber and seal the edge with a small amount of agarose solution.Place the comb and adjust the distance between the bottom edge of the comb and the electrophoresis plate, generally 1-2 mm is appropriate. 3) When the dissolved agarose solution is cooled to about 50C, add 5 M L of ethidium bromide, and the final concentration of ethidium bromide is 1.0 m g/m L. After mixing, pour the agarose solution into the electrophoresis plate and keep it still without moving. 4) After the gel has completely solidified (30-45 minutes at room temperature), gently remove the comb, remove the tape, and place the gel in the electrophoresis pad. 5) In the electrophoresis process, electrophoresis solution (1'TAE) was added to cover the agarose gel surface with about 1-2 mm electrophoresis solution.

3-(cyclohexylamine)-1-propanesulfonic acid, also called CAPS, is a kind of biological buffer, which is commonly used in biochemical diagnostic kit, DNA/RNA extraction kit and PCR diagnostic kit. The buffer used for enzyme chemistry and HPLC separation of basic drugs is relatively stable, with pKa value of 10.4 and pH buffer range of 9.7-11.1 at 25℃. It is widely used in biochemical analysis and in vitro diagnosis.   CAPS in carton   In addition to the biochemical industry, CAPS is widely used in the following fields:   1. CAPS is widely used as biological buffer: in biochemical diagnostic kit, DNA/RNA extraction kit and PCR diagnostic kit; in enzyme chemistry and HPLC buffer for separation of basic drugs. When caps is used as biological buffer, it needs better purity. Generally, it needs analytical purity. When it is used in industry, the purity requirements are relatively low. However, different treatments should be made for different applications.   2. In industry, CAPS is used for new coatings and materials. CAPS is also the raw material for manufacturing welding materials, air conditioning equipment and lithium metal.   3. It is used as analytical reagent, heat exchange carrier and pharmaceutical industry. It is used for air conditioning, fireworks, dry batteries and lithium metal, as flux and desiccant.   4. For coating industry, it is used as curing agent of water-based isocyanate. The water-based isocyanate curing agent can coexist with resins containing active groups (hydroxyl, carboxyl, amino, epoxy, etc.) for a long time at room temperature.   CAPS has such a wide range of uses and many manufacturers. When selecting manufacturers, attention should be paid to identify qualified manufacturers and avoid intermediaries to make profits from them. Hubei New Desheng Materials Technology Co., Ltd. is located in Guanggu United Science and Technology City C8-2, Gedian Development Zone, Ezhou City, Hubei Province. It has 14 years of research and development and production experience in the field of biochemistry and specializes in the production of biological buffers.The enterprise is ISO 9001 quality management system certification approved and has a good reputation in the industry. It is a trusted manufacturer of biochemical raw materials. It is absolutely wise for you to choose Desheng.

Polyurethane coating is a kind of coating technology which began to rise in the 1960s. In view of the increasingly strict requirements of environmental protection laws and regulations, the environmentally friendly water dispersive polyisocyanate has shown its importance in various application fields in recent years. Although polyether modified polyisocyanates are widely used, they all have one basic disadvantage, especially when they are used as crosslinking agent of waterborne two-component polyurethane coating, higher polyether content is needed to ensure sufficient dispersion, which leads to a long drying time and long-lasting hydrophilicity of the coating. These shortcomings have been solved on the CAPS (3-(cyclohexylamine)- 1-propanesulfonic acid) modified polyisocyanate.   CAPS   CAPS (an amphoteric sulfamate) reacts with aliphatic polyisocyanate under mild conditions and in the presence of tertiary amine neutralizer. The sulfonylurea derivative is an excellent emulsifier. Without considering the influence of salt forming group, CAPS modified polyisocyanate has very good storage stability and the finished product is not turbid. Even if it contains less sulfonate groups, it can also be in water The emulsion with good dispersing state was obtained. Thus, a series of ionic modified polyisocyanates can be obtained, which can be used in various environment-friendly high-quality two-component polyurethane coatings. These coatings are completely superior to general solvent based coatings in terms of dryness, curing and chemical resistance. With the requirements of new regulations, VOC (volatile organic compounds) content in decoration materials is further reduced, which makes the amount of these cross-linking agents increase greatly. Compared with solvent based coatings, they will not lead to the degradation of film quality. CAPS modified polyisocyanate has been widely used in automobile original paint, repair paint, plastic paint, wood paint, fabric leather paint, printing ink industry, etc. with its excellent performance. The market prospect is self-evident.   Preparation of CAPS modified polyisocyanate   950g polyisocyanate was stirred with 50g CAPS, 29g dimethylcyclohexylamine and 257g 1-methoxypropyl-2-yl acetate under dry nitrogen for 5 hours at 80 ℃, wherein the polyisocyanate is based on hexamethylene diisocyanate (HDI) and contains isocyanurate group, NCO content is 21.7%, average NCO function is 3.5, monomer HDI content is 0.1% and viscosity is 3000mpas. After cooling to room temperature, colorless and transparent polyisocyanate solution can be obtained.   CAPS produced by Desheng company has not only been widely used in the new paint market, but also in the field of biochemical industry. Because it is also a biological buffer, it is widely used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. Welcome enterprises and individuals to call for turther consultation.