China Wuhan Desheng Biochemical Technology Co., Ltd Company News

  Tris(hydroxymethyl)aminomethane is also abbreviated as tris base, CAS77-86-1, alias tromethamine, ammonium tromethamine, trimethylaminomethane, is a commonly used biological buffer, conventionally produced as white crystalline powder, PH value Weak base, the buffer range is between 7.0-9.2.   Application of Tris in activated carbon   Modern people pay more and more attention to the quality of life, but many living environments and office environments have more or less harmful gases such as formaldehyde and benzene. The use of activated carbon has become the choice of many people, using activated carbon to adsorb these harmful gases. Among them, tris is used as modifier, and then through hydrothermal reaction, the denatured activated carbon is better used in life.   Preparation method of Tris in activated carbon   What is introduced here is the method of adsorbing trace sulfur dioxide by modified activated carbon fiber, which belongs to gas adsorption and separation technology. Using tris, serine, alanine, etc. as the basis to make modifiers to treat viscose activated carbon fibers, through hydrothermal reaction under fixed conditions, to prepare chemically modified activated carbon fibers with a large number of amino groups on the surface.   Preparation by this method has the following advantages: (1) Small molecules are used to ensure the modification of the modifier surface, and the high ratio of activated carbon fiber is maintained, so that the porous structure in the activated carbon will not be destroyed, thereby better adsorption The harmful substances in the air increase the amount of adsorption; (2) This method is relatively mild, has less pollution, is easy to scale up production and guarantees the environment to a certain extent; (3) Use tris (hydroxymethyl) aminomethane and other chemical reagents to modify After modified activated carbon, its adsorption capacity can be increased to 40-60mgSO2/g adsorbent, which is 4-6 times higher than before modification.   Desheng tris meets the requirements for activated carbon modification   In fact, the number of tris manufacturers on the market is decreasing, not only because of the relatively serious safety accidents in 2019, but also because of the emphasis on the environment, many manufacturers actually cannot meet certain requirements. Desheng Biochemical has been testing tris since 2012. It has a professional R&D team, and has accumulated experience in continuous experiments. According to market requirements, it has invested in pilot and large-scale trials, and it has the current large-scale production. The amino methane products are adapted to various needs, and there is no problem with modified activated carbon. Special indicators can also be customized! The quality of a manufacturer can be seen from the receipt of the goods, Desheng welcomes your consultation!      

Carbomer is a high molecular polymer crosslinked by acrylic acid and allyl sucrose or allyl pentaerythritol. It was first produced by g00drich company of the United States, and its trade name is carbomer. Chinese Pharmacopoeia 2005 edition has been included. This product is a loose white powder, with acidity, hygroscopicity and special odor. It is soluble in water, ethanol and glycerin.   The advantages of carbomer are good gel, sticky, thickening, emulsifying, suspending and film-forming properties. The chemical properties are stable, safe, non irritating and hypersensitive. They are very compressible under low pressure and are compatible with most other excipients.   It can increase the bioavailability and show a good correlation in vitro and in vivo. The drug preparation with zero order release rate can be made. It can form soluble gel type internal salt with basic drugs, so that the drug can release slowly. Salt electrolytes can reduce the viscosity of carbomer gel. Alkaline earth metal ions and polymers can be combined to form insoluble salts. Strong acids can also cause carbomer to lose viscosity, and must be avoided in compatibility.   Disadvantages: 1, hygroscopicity, easy to caking, storage should pay attention to moisture. 2. Carbomer dry powder and high concentration mucilage are irritating to eyes, nose and throat. After contact, it can be rinsed with physiological sodium chloride solution. 3. Non solid state has no antibacterial effect, but does not support the growth of bacteria, so preservatives should be added.   carbomer gel   Because of its advantages, Carbomer is widely used, but it also has disadvantages, among which group formation is the problem that most customers respond to. Many manufacturers will encounter various problems when using Carbomer, because carbomer has strong hydrophilicity. Carbomer of dry powder has strong hygroscopicity. Like other hygroscopic powders, it is easy to form clusters or incomplete wetting when it is put into water or other polar solvents by improper methods.   Other powders will eventually decrease and dissolve after agglomerating, but carbomer will not dissolve easily after agglomerating, because once the outer layer of the agglomerate is completely infiltrated, water is not easy to penetrate into the internal dry part, and finally agglomerate.   Desheng, as a professional manufacturer of cabom, according to the experience of engineers in the technical department, sorts out several methods here for reference. 1. Sprinkle carbomer on the water (Note: carbomer sprinkles on the water, not carbomer adds water), and keep it still for one night to make it fully dissolve and swell; 2. Grind carbomer and glycerin (or propylene glycol, according to the prescription) in mortar, and then add water to grind evenly; 3. Add a certain amount of water into the mixer, slowly add carbomer under rapid stirring, and continue stirring for 1-2 hours after adding to make it fully dissolve;   Desheng friendly reminder:   1. If it is a laboratory test, it is recommended to use 2 and 3 methods;   2. If it is a pilot plant or production, it is more appropriate to use methods 1 and 3. If method 1 is used, there may be jelly like lumps, but it is not a big problem: after a colloid mill, a very uniform colloid can be obtained. After the colloid mill, there may be more bubbles, which can be eliminated by vacuumizing and placing overnight;   3, the above method is only to get carbomer colloid. To get gel matrix, we must adjust PH to 6-10 by triethanolamine or sodium hydroxide solution. The resin particles must be evenly dispersed in cold water. Under the high speed of 500-800rpm, the carbomer can be sieved into the stirring vortex by screen. The optional dispersion equipment can be ejector, flocculation disperser and conventional disperser.   The methods explained above are purely personal experience. The Carbomer manufacturing equipment used by each company is different, and the methods vary from person to person. If you know other good ways to avoid kapom forming a group, you are also welcome to leave a message for Desheng to learn together. Kapom has many different models. Desheng's current sales volume and feedback are Carbomer 980 and Carbomer 940, which are available from stock. Welcome to contact us Inquiry and order!

Situation Analysis of Tris   In the past few years, the development speed of tris base (tris(hydroxymethylaminomethane)) CAS77-86-1 is very fast and has achieved visible growth. This is not only the case in China, but also in North America, Europe, the Middle East, Africa and other regions. Large companies in many countries directly use mergers and acquisitions to promote the growth of the entire market.   The relatively large manufacturers of tris hydroxymethylaminomethane have certain plans for market liquidity and future development. Along with different influencing factors, all will be directly related to the market of tris. More new tris production capacity will also get new opportunities.   Factors affecting tris   TRIS (Tris(hydroxymethylaminomethane)) (CAS 77-86-1) market growth drivers, constraints, future opportunities and challenges. The use of tris in biological buffers is more and more widely used, and there are no alternative biological buffers, making the use of tris indispensable.   The limiting factor lies in the timely supply of raw materials. Compared with domestic considerations of environment and safety, more foreign sources are the timely supply of various raw materials. The future development of tris will be more rapid, but it will benefit from good manufacturers and good product quality.   The Challenge of Tris   Facing the demand for a large amount of tris, the manufacturers of tris is more need to consider how to produce the product more efficiently and ensure product quality. Large-scale production of TRIS (Tris(hydroxymethyl)aminomethane) (CAS 77-86-1) will be a better way to reduce costs, and improving the production process is also an issue. Although the competition is fierce, benign stimulation is necessary.   The impact of the new crown epidemic on the TRIS (Tris(hydroxymethyl)aminomethane) (CAS 77-86-1) market The impact of the COVID-19 epidemic on the tris is undoubtedly huge. It brought the global economy into a deadlock suddenly and caused a catastrophic disaster. However, there are also new opportunities in the tris. The newly developed virus preservation solution often requires the tris for debugging. Compared with other products, tris has shown some growth.   Global TRIS (Tris(hydroxymethyl)aminomethane) (CAS 77-86-1) market   North America (U.S., Canada, Mexico) Europe (Germany, United Kingdom, France, Italy, Russia, Spain and the rest of Europe) Asia Pacific (China, Japan, India, Russia and other Asia Pacific regions) Latin America (Cuba, Brazil, Argentina and The rest of Latin America) the Middle East and Africa (South Africa, the Gulf Cooperation Council, and the rest of the Middle East and Africa).   As a professional manufacturer of tris (tris (tris(hydroxymethylaminomethane)) CAS77-86-1, Desheng Biochemical not only maintains the quality of the products, but also pays attention to the timely supply of products. Friends in need can contact Desheng!

The 2019 new crown pneumonia (COVID-19) is affecting the entire global market. In addition to the cost of human life, the impact of the spread of the virus on the global economy has begun to be recognized and has a profound impact on the world's technology supply chain. Measures such as large-scale quarantine, travel restrictions, and social isolation have led to a sharp drop in personal and corporate spending. Companies lose income, lay off employees, and unemployment has risen sharply. Carbomer was once the darling of daily chemical products due to its application as a hand sanitizing gel. It is precisely because of the surge in market demand that carbomer was out of stock for a time.   Facing this severe situation, Desheng looked for opportunities in adversity, established a technology research and development center, increased investment in research and development, and received a good market feedback. There are currently hundreds of cooperative enterprises, so why Carbomer With so many manufacturers, does the customer prefer AdSung? Here are the following points.     1. Is a real manufacturer, not a trader It is also necessary to understand the supply background. Although there are some domestic manufacturers that produce carbomers, some of them are not of good quality, and some are not specialized in producing this product. Perhaps because they think it is the general trend, they did not spend too much. Human, material and financial resources to develop and produce, if the inventory is not enough, this will also bring a lot of inconvenience to subsequent cooperation, and the real manufacturer will not worry about this problem, for example, Desheng has its own professional R&D team. There is no need to worry about the quality of the product at all, and if there is a problem, you can return it without worry.   2. Product trial available If the product is good or not, you can tell when you try a sample. Nowadays, when looking for a product, some purchasers usually communicate in words first, and then communicate by phone when they think it’s suitable. This is actually low efficiency. More importantly, some customers don’t pay attention to the performance parameters of the product at all. Ask the price, if the price is right, if you don’t want to buy it, then you don’t need to talk about it. At this time, it is a very good experience to provide samples for trial. Customers will no longer struggle with the price. If the trial is good, they will naturally "visit" again, and they will naturally choose another home if they are not used well. . Desheng has done statistics, as long as the customers who have tried the product have 100% repurchased,   3. High daily output and fast delivery Many customers’ order quantity is very large. If Carbomer’s inventory is not enough, it will inevitably lose many important customers. Desheng has a professional production base and uses imported production equipment. The daily output can reach 3-5 tons. Satisfy customers' large-volume ordering needs, with good thickening performance, high transparency, and high cost-effectiveness. Customers no longer have to worry about the delivery cycle, because Desheng has the ability to deliver quickly.   During the epidemic, many companies closed down or fired their employees due to business stagnation. This phenomenon has never happened in Desheng. As a professional carbomer manufacturer, Desheng can provide high-quality products and services compared to other manufacturers. More cost-effective, the price is as low as 130 yuan/kg for ton and above. With the public efforts of all employees of the company, our carbomer sales continue to rise, and our products have been unanimously recognized by customers. At present, we mainly sell carbomers There are two models of 940 Carbomer 980, with business in more than 30 countries.

There are many different colors for disposable vacuum blood collection vessels. Different colors of the head cover indicate that the anticoagulant or accelerator added in the blood collection vessels are different, so the corresponding functional application is also different. Different colors of the head cover and labels can also strengthen the identification to prevent the wrong use of blood vessels.   1、 Coagulation promoting tube with golden safety head cover   Inert separation gel and accelerator were added into the blood collection vessel. After centrifugation of blood samples, the inert separation gel can completely separate the liquid components (serum or plasma) and solid components (red blood cells, white blood cells, platelets, fibrin, etc.) in the blood, and completely accumulate in the center of the test tube to form a barrier. The samples remain stable within 48 hours. Coagulant can quickly activate coagulation mechanism and accelerate coagulation process, which is suitable for emergency serum biochemical test.     Different anticoagulants and accelerators for blood collection   2、 Purple safety head cover, EDTA anticoagulant tube   EDTA tube is used for blood routine test of blood examination. EDTA chelates with calcium ion in blood to inhibit coagulation process. It is suitable for general hematology test, not for coagulation test and platelet function test, nor for determination of calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine aminopeptidase, and PCR test; it has the least effect on red blood cell morphology, and can inhibit platelet aggregation. The spray method is usually used to make the reagent uniformly attached to the tube wall, so that the blood sample can be quickly and fully mixed after sampling.   3、 Green safety head cover, heparin anticoagulant tube   Heparin sodium or heparin lithium can prolong the initial clotting time. Heparin anticoagulant tube is suitable for red blood cell fragility test, blood gas analysis, hematocrit test, erythrocyte sedimentation rate and biochemical determination. It is not suitable for hemagglutination test. It has less interference to blood components, does not affect the volume of red blood cells, and does not cause hemolysis.   In addition to the above three kinds of blood collection vessels, the common ones are the red head cap tube without additives, the blue head cap sodium citrate anticoagulant tube, and the black head cap blood sedimentation tube. According to the sales situation of Desheng's blood collection vessel additives, the above three kinds of blood collection vessels are the most widely used.

Chroma TOOS:   TOOS has a higher molar absorbance of the color product, which means that the sensitivity of the color reaction is higher, and it can be used for the determination of very small amounts of test items in the serum. The absorbance of the color reaction product formed by TOOS does not change much with time, that is, the color reaction product is stable and not easy to fade.   Precautions for using TOOS   TOOS is reducible. Although peroxidase is needed for detection, the enzyme is its catalytic function. Without enzyme, it may be slowly oxidized by oxygen in the air after being prepared into a solution. . The chromogen substrate needs to be prepared into a solution when used. The chromogen substrate produced by Desheng is a pure white powder, sealed and stored. After the solution is prepared, it should be prepared for immediate use. It will be slowly oxidized and discolored if it is in contact with air for a long time.   Chroma MAOS:   The absorption wavelength of chromogen MAOS is higher, reaching 630nm, which is less interfered by other substances in serum or other samples, and can be targeted for detection items that require high-precision values. However, the product of MAOS fades significantly, and the data needs to be observed in time during the test. Therefore, the MAOS test must be completed in time without interruption. Furthermore, the color reaction of MAOS has a wide adaptability to the pH of the reaction system, which greatly improves its adaptability. The reaction of some color reagents is often under acidic conditions, but biochemical detection usually requires the participation of enzymes. We know that the catalysis of enzymes is more sensitive to the pH of the reaction environment, and does not match the color reagents. Inactivation will be greatly restricted in use, but MAOS reagent does not have this problem.   Precautions for using MAOS   As MAOS is sensitive to light and humidity. Therefore, when we store, the storage temperature of refrigeration is between 0-10, and the temperature of frozen storage is below 0. If the storage temperature is not indicated, it is best to store in a cool place below 15 in principle. In principle, the expiration date of the product is because the reagents may change over time due to storage conditions or trace impurities mixed into it. Therefore, it is difficult to strictly determine the expiration date or shelf life.

Carbomer is a kind of acrylic resin. It is a white loose powder with strong hygroscopicity. Its molecular structure contains 52% ~ 68% carboxyl group. In water dispersions, a transparent gel can be formed by neutralizing alkaline substances with a non sticky feel. Carbomer has thickening effect in a wide range of pH.   It has the functions of thickening, suspending, emulsifying and pseudothixotropy, regulating the release of water and active substances, etc. it is a rheological modified thickener widely used in cosmetics.   Thickener is an important part of cosmetic formula, which not only makes it easy for the formulator to change the viscosity of the product, but also improves the stability, aesthetic feeling and fluidity of the product.   Carbopol gel   At the same time, Carbopol resin used for liquid thickening has the following advantages:   ① It does not irritate people's skin and eyes. After a lot of research, it is found that it is safer than salt and has been used for more than 50 years;   ② Compared with cellulose and guar gum, the suspension stability of silicone oil was higher;   (3) improving foam stability in low foam system.   ④ The results show that the cross-linked copolymer of acrylic resin can maintain high viscosity in the presence of salt, and is less affected by the pH value or electrolyte concentration of the solution, so it can be widely used;   ⑤ Easy to use, short wetting time, saving a lot of manpower, material resources and time;   ⑥ Good corrosion resistance, not easy to be decomposed by microorganisms, easy to preserve;   It has excellent rheology, which can adjust the viscosity of products, improve thixotropy of gel system, and improve product stability.   Only a small amount of carbomer can reach the required viscosity. The conventional dosage is 0.1% to 0.5%, and has the effect of thickening and stabilization. Thus, emulsion, cream and gel products with wide viscosity range and different rheology are prepared.   Another key characteristic of carbomer is its high fluidity, which can make the insoluble components suspend permanently in the low concentration system and make the product uniform and stable, while other thickeners such as cellulose can not provide suspension.   Desheng Technology specializes in the production of carbomer 940 and 980, with advanced production equipment and mature production technology, with an average daily output of tons. The carbomer powder produced by the company is whiter and more fluffy than that on the market, with better transparency after dissolution. According to the customer response, the effect of product use is good, can support free application sample trial, welcome to inquire and order!

Acridine ester DMAE-NHS is a typical acridine chemiluminescence reagent that has been commercialized. It has the characteristics of high sensitivity of chemiluminescence analysis, simple equipment, no light source to avoid the influence of astigmatism, and high reproducibility. Important fields such as environmental monitoring, clinical analysis, and biochemistry have been widely used.   Acridine chemiluminescence reagents for detection Development of acridine chemiluminescence reagents: Acridine Since Grebe and Carlo discovered acridine in the high-boiling fraction of coal tar in 1870, acridine and acridine derivatives have attracted a lot of scientific and technological interest, and have subsequently been widely used in the development of anti-cancer drugs and antibacterial agents. , Immunodiagnosis and other fields. Acridine chemiluminescent reagents are a technologically advanced luminescent marker for proteins, antibodies, and nucleic acids in immunodiagnosis.   Advantages of acridine ester DMAE-NHS:   Compared with other luminescent substrates such as luminol and AMPPD, acridine-based luminescent reagents have high luminescence intensity, high efficiency, low background interference, no need for enzyme catalysis and enhancers, high signal-to-noise ratio and label binding Stability and other advantages. Moreover, after structural modification of acridine compounds, a chemiluminescence probe is made, which is not only suitable for labeling small molecules, but also for labeling biomacromolecules with chemiluminescence, which can label proteins, proteins under neutral or slightly alkaline conditions. Biological macromolecules such as nucleic acid and polypeptide, and the fluorescence quantum yield and biological activity of the labeled substance will not be damaged.   Comparison of acridine ester DMAE-NHS with other acridine luminescent reagents:   There are more than ten kinds of acridine chemiluminescence reagents commercialized on the market, of which 6 are developed and produced by Desheng, including 3 acridine esters and 3 acridine sulfonamides. Compared with other luminescent reagents, the acridinium ester DMAE-NHS is characterized by containing NHS groups, which can be directly connected to the primary amino groups of proteins or antibodies for labeling. There are also some differences in hydrophilicity from acridine sulfonamides. Choose the appropriate acridinium ester for the type of protein or antibody.   It should be noted that Desheng’s acridinium ester DMAE-NHS has improved hydrophilicity on the basis of traditional acridinium ester, but its dissolution process still needs to be strictly anhydrous. In addition, mercapto groups may cause acridine ester hydrolysis. Mercaptoethanol cannot be used, and care should be taken if the labeled protein contains sulfhydryl groups.

  Carbomer 940 is used in jelly gel formation. It can be used as an excellent floatation agent, stabilizer, emulsifier and matrix for pharmaceutical excipients. It is also an effective water-soluble thickener. 0.5% of the added amount can make jelly gel with jelly effect, slightly acidic, and the pH value of the dispersed system in 0.5% water is 2.7 - 3.5.   Recommended dosage of carbomer:   Generally, the addition amount is 0.2 to 1.0%, and in most cases, 0.2 to 0.5%. The 0.2 to 2% amount of carbomer resin is used as an emulsifier. The amount of 0.2 to 2% of carbomer resin is used as thickener. The 0.2 to 2% amount of carbomer resin is used as ointment. The 0.2 to 2% amount of carbomer resin is used as a gel.   carbomer gel     How to use carbomer 940:   2% carbomer gel (50ML):   1. Weigh 1g carbomer dry powder and place it in a dry beaker with a scale of 50ml. If there is caking, please grind it with a spoon.   2. Add 25ml pure water, stir while adding, make carbomer fully absorb water and dissolve. This step is very critical. You must make carbomer absorb water fully. Generally, after adding water and stirring, you can put it for a few hours (you can configure it before going to bed at night, and it can be deployed the next day). Carbomer can fully dissolve. If you want to speed up, you can use a water bath to heat. It will be faster to stir while heating. Of course, even if heating can accelerate the process of carbomer's water absorption and swelling, it will take 10-30 minutes.   3. Drop the lye with the drop tube and stir it side by side to measure pH value. When the PH7 is adjusted, the gel is successful. (triethanolamine is enough for lye).   matters needing attention: 1, Carbomer must interact with the alkali triethanolamine to form a solid gel texture. 2. It is not resistant to acids, electrolytes, salts and other substances. Once it meets with salt, it will decompose into water   Product packaging Packaging and storage:   Carbomer has a strong hygroscopicity. When storing, pay attention to sealing, and be careful to avoid moisture absorption and caking in the exposed air. If there is slight caking, it can become loose powder by gently patting and pressing, which will not affect the thickening effect. Once the product is used, it is best to place it in a sealed container with desiccant.   Transportation:   Desheng carbomer is packed in non-toxic polypropylene and nylon co extruded plastic bags, moisture-proof and airtight, and then packed in cardboard boxes. Carbomer is a polyelectrolyte, and sometimes static electricity is carried by rubbing or extruding resin particles. In case of spilling, please sweep up the dry powder with a broom first, and do not wash it with water, otherwise it will form a very slippery thin layer on the ground or equipment.   Desheng carbomer is more than 100 yuan / kg in tonnage, with large quantity and more favorable price. The carbomer 940 and 980 powders produced by Desheng carbomer are whiter and more fluffy than those in the market, and the transparency after dissolution is better.  

Dipotassium ethylenediaminetetraacetate is usually referred to as dipotassium EDTA. When purchasing reagents, we will find that it has two CAS numbers. One of these is actually EDTA dipotassium crystalline hydrate, but many people on the Internet confuse the two. , It becomes a mixed use of the two numbers. What is the difference between the two and will it affect the use?   The dipotassium EDTA is CAS 2001-94-7, and the dipotassium EDTA dihydrate is CAS25102-12-9. The difference between the two is that one molecule has two crystal waters. Dipotassium EDTA powder is easy to absorb moisture and become crystal water. It is relatively stable when it contains two crystal waters. For blood anticoagulants, EDTA dipotassium containing crystal water is generally used.     Purchase details of blood anticoagulant EDTA dipotassium   Other anticoagulants such as tripotassium EDTA and sodium citrate have similar situations. In fact, when most salts or other compounds precipitate crystals from aqueous solutions, the crystals often contain a certain number of water molecules, which is crystal water; many mineral salts in nature are more stable when they exist in the form of crystal hydrates.   The number of crystal water of crystal hydrate is usually related to the vapor pressure of water in the environment. As the vapor pressure increases, the number of water molecules also increases. The stability of crystal hydrate is related to temperature, cation and anion. Generally, potassium ions do not have crystal water (dipotassium EDTA and tripotassium EDTA both have crystal water, with exceptions). Sodium ions often have crystal water, such as their carbonates. The number of crystal water in salts is related to temperature, 34 There are 10 crystal waters below ℃, and no crystallization water above 34℃. Some crystal hydrates will automatically lose crystal water in dry air, that is, weathering; some will automatically lose crystal water, deliquescence or moisture absorption.   When used as a blood anticoagulant, EDTA dipotassium or tripotassium both need to be prepared with water to prepare a solution. Whether it contains crystal water will not affect its anticoagulant effect. However, in the test and calculation, the molecular weight of EDTA dipotassium without water and with crystal water is different. Dipotassium without crystal water has stronger moisture absorption and is not as stable as hydrate. If it absorbs moisture, it may cause calculation errors.   Special attention should be paid to the EDTA dipotassium CAS number when purchasing. Don't think that the CAS number is not the same as if the CAS number is inconsistent. Be sure to check repeatedly. The EDTA dipotassium, tripotassium, and sodium citrate produced by Desheng are easy to absorb moisture in the air, so it is sealed and stored. Please pay attention to it when using it.

Agarose gel electrophoresis is a commonly used method for isolation, identification and purification of DNA fragments. When DNA molecules are swimming in agarose gel, they have charge effect and molecular sieve effect. DNA molecules are negatively charged in pH solution above isoelectric point and move to the positive electrode in the electric field. Due to the repetitive structure of the glycophosphate backbone, the same amount of double stranded DNA has almost the same net charge, so they can move to the positive direction at the same speed.   Different concentrations of agarose gel can separate DNA fragments from 200bp to 50kb. In the agarose solution, low concentration of ethidum bromide (EB) was added into the agarose solution. The DNA band of 10NG could be detected under UV light. In the electric field, the negatively charged DNA migrated to the anode in pH8.0.     Agarose gel has the following characteristics: (1) the molecular size of DNA in the gel matrix is inversely proportional to the value of the base logarithm, and the larger the molecule, the slower the migration.   (2) agarose concentration in a specific size of linear DNA molecule is different from agarose gel in different concentrations. The logarithm of electrophoretic mobility (U) of DNA is linearly related to gel concentration (T).   (3) When the voltage is low, the migration rate of linear DNA fragments is proportional to the applied voltage. However, with the increase of electric field intensity, the mobility of DNA fragments with different molecular weights will increase in different ranges. With the increase of voltage, the effective separation scope of agarose gel will be reduced. In order to maximize the resolution of DNA fragments larger than 2KB, the applied voltage should not exceed 5V / cm.   (4) electrophoretic behavior of electrophoresis temperature DNA in agarose gel electrophoresis is not affected by the temperature of electrophoresis. The relative migration rate of DNA fragments of different sizes does not change significantly between 4 and 30 degrees, but gel with low concentration of 0.5% or low melting point gel is relatively weak, preferably at 4 degrees.   (5) dye embedded fluorescent dye ethidium bromide was used to detect DNA in agarose gel. The dye was embedded in the stacked base pairs and elongated and notched circular DNA, making it more rigid and reducing the linear mobility by 15%.   (6) The composition of ionic strength electrophoresis buffer and its ionic strength affect the mobility of DNA electrophoresis. In the absence of ions, such as misuse of distilled water to prepare gels, the conductivity is minimal and DNA hardly moves. In high ionic strength buffer (such as 10 x electrophoresis buffer), conductance is very high and obvious heat production.   Agarose gel electrophoresis is also commonly used in isolation and identification of nucleic acids, such as DNA identification, DNA restriction endonuclease map production, etc. Because of its convenient operation, simple equipment, small sample size and high resolution, this method has become one of the common experimental methods in genetic engineering research. Agarose gel electrophoresis kit contains 10 agarose preforms, high voltage fast electrophoresis solution 15mL, DNA loading buffer6x 200 L, scissors and user manuals, with 8 hole 6*6, 6 hole 6*6, 13 hole 6*12, 18 hole 6*12 and other specifications.

For many people, especially women, a good skin condition is very important. Many skin topical medicaments contain a special component, Carbomer 940. Although this professional term sounds strange to us, its application to skin is very extensive, mainly due to the gel produced by carbomer 940 for skin. There are many advantages.   Some experts have prepared Allantoin Gel with 1.5% carbomer 940 to treat skin diseases such as dryness and psoriasis. The clinical results show that the gel has good compatibility with the skin, has a lasting effect and has no greasy and stuffy feeling on the skin. Amin and other erythromycin gel prepared by 1% carbomer 940 were used to treat acne. The results showed that the number of acne and basal diameter were significantly reduced.   carbomer gel   The ultrasonic diagnostic gel made by carbomer 940 has the advantages of no irritation to human skin, fine appearance, fine texture, no damage to the probe, no pollution, good lubrication, good viscosity and stable preparation. It has been proved by clinical practice that all the quality indicators achieve the desired effect.   In addition, the preparation of ketoprofen from 4 different substrates showed that the drug was released most rapidly from carbomer gel. The release rate was carbomer gel > hydrophilic ointment > cold cream > white vaseline. It suggested that carbomer could promote the transdermal absorption of drugs.   Carbomer 940 gel matrix has the advantages of simple operation, stable quality and no need of open flame heating compared with Vaseline matrix and cream substrate. It is especially suitable for the preparation of volatile drugs with poor thermal stability and small quantities of urgent drugs.   Desheng is a manufacturer specializing in the production and development of carbomer 940. Since its launch, cooperative enterprises have exceeded 100+. So many companies have chosen to cooperate with De Sheng to show that our products are better than those of other manufacturers. The gel matrix made from the raw product of Kam Sheng 940 has the characteristics of water-soluble matrix, quick release, no greasy, easy to spread, and skin. Mucous membrane is not irritant, welcome the manufacturers who have great demand to choose to cooperate with Desheng.