China Wuhan Desheng Biochemical Technology Co., Ltd Company News

Generally to the hospital, say nothing, the doctor will let you do a blood test, check whether there is a problem with blood. Why is blood test so important and common? What does it know about the body? How to read the blood routine report without a doctor? Mo Ji, Wuhan Desheng biochemical technology specialized in the production of in vitro diagnostic reagents for 12 years, let you read this article, absolutely understand! Key points of this paper: ·Why does the doctor always ask me to check the blood? ·What's in the blood? ·What index should blood routine check? ·What is the significance of changes in indicators? The continuous circulation of blood in the human body plays an important role in ensuring the balance of metabolism, environment and function regulation. Pathological changes of any components in the blood will affect the tissues and organs of all parts of the body. On the contrary, pathological changes of organs or tissues will also cause changes in blood composition to a large extent. In addition, checking the change of blood composition can also find the cause of disease for treatment, which is helpful for doctors to judge correctly. In the past, children had a cold. They went to the hospital directly to hang liquid medicine. As a result, many times they took medicine. The cold was not good. Now it's different. To go to the hospital, you need to take a blood sample first to determine whether the cold is caused by a virus or a bacterium. Then the doctor diagnosed and treated according to the results of blood test, the effect was naturally good. Therefore, the significance of blood test should not be underestimated. Why to draw a little blood, can know the body each part has what unusual? Before you answer this question, you need to know what's in your blood.

Application of luminol in chemiluminescence analysis Luminol chemiluminescence analysis has the advantages of high sensitivity, wide linear range, fast analysis speed and relatively simple instrument equipment. It is widely used in the fields of life science, environmental science, clinical medicine and physiology. Chemiluminescence analysis in addition to many efficient separation methods such as combination of high performance liquid chromatography (HPLC), capillary electrophoresis, etc, in recent years, it also and flow injection analysis, electrochemical analysis reagent, biological immune analysis, immobilized technology, sensor technology, analysis technology, the rapid development, become one of the hotspots in the field of chemical analysis. Luminol has become one of the most widely used chemiluminescence reagents due to its advantages of high quantum yield, easy synthesis and good water solubility. Zhang zhujun et al. made a detailed review on luminol chemiluminescence reaction and its application in analysis. The analytical application of luminol - iodide chemiluminescence system is introduced. However, due to the slow reaction rate of luminol oxidation luminescence, it is necessary to add some enzymes or inorganic catalysts. The detection of analyte by luminol chemiluminescence and reagent immobilization is an important research direction. A chemiluminescence sensor for the determination and measurement of glucose was designed by using a light-emitting system, which was fixed on the carrier. A sensor for the determination of riboflavin and rutin was designed based on a similar method. A time - resolved chemiluminescence analysis method for the determination of homobarine and homobarine in binary mixtures was proposed based on the different catalytic rates of peroxidase to the oxidation of the amine compounds. Due to the stable performance and low price of luminol, practical application research and analysis will have a broader application prospect. Another major use of chemiluminescence in immunoassay is immunoassay. Chemiluminescence immunity in which a luminescent reagent or sensitizer molecule is labeled on an antigen or antibody for enzymatic, antigen, or antibody determination. Application of luminol in organics analysis The organic compounds can be analyzed and determined by the promotion and inhibition of luminol luminescence system. Recently, the inhibitory effects of different antioxidants on superoxide anion expansion, radical transradical, peroxynitrite and luminescence of luminol were systematically investigated. The free radical scavengers in plant extracts were determined based on the inhibition of the antioxidant system and in combination with the technique. A new method for the determination of hemorazine and hemorazine has been developed based on the properties of the potential antioxidant hemorazine, which promotes the yao 10 system, and the glutorazine has an inhibitory effect on the fumino nitrite system. Based on the promotion of a luminescence reaction, the chemiluminescence method for the determination of isopropyl throat, chloropropyl throat, vanilla amygdalic acid, isosmoke well and skin was established based on the inhibition of the system. Based on the properties of peroxide dismutase inhibiting luminol superoxide anion chemiluminescence, xu et al. established a simple method for determination. The content of penicillin 1 in human serum was determined based on the promotion effect of penicillin 1-10 system and the high-efficiency head-on analysis technique. Based on the inhibition effect of the system, theanine in tea was determined with the linear range of a. Based on the inhibition of catechin on yi-yi system, the catechin was measured and the oil was detected. Based on the inhibitory effect of p-ethyl-aminophenol on a luminescent system, an analytical method for the determination of p-ethyl-aminophenol in the drug was established, with a linear range of one. Years old. Recently, based on the inhibition of protein on luminol - tetrasulfonic manganese phthalide blue hydrogen peroxide luminescence system, a new high sensitive chemiluminescence method for the determination of protein was established in combination with flow injection. Many clinically important substances, such as glucose, urea, uric acid, lactic acid, choleol, choline, catecholamines and some enzymes and their substrates, can be produced by certain reactions. Indirect determination of these substances can be realized by the luminescence detection of the generated substances. Applications of luminol in pharmaceutical and environmental analysis The application of luminol in drug and environmental analysis and the study of its reaction mechanism include two parts: review of chemiluminescence analysis and research report. In 1877,Radziszewski discovered that loronine glows green when oxidized in alkaline media by reagents such as hydrogen peroxide. Since then, many similar luminescent compounds have been synthesized, and their luminescence mechanism and application have been further studied. DE sheng technology since the 05 years began to research and development production of luminol, luminol, separation of glue, heparin, EDTA potassium collects blood vessels such as additive, and in vitro diagnostic reagent, the color of the original substrate TOOS, MAOS new Trinder 's reagent, Bicine and Tris biological buffer and luminol, acridine esters such as chemiluminescence reagent has a deep research, the independent research and development and synthesis of professional advantage, DE sheng technology to become the industry's leading brand of the world, to make great contributions to human health. At present, the company has reached a long-term cooperative relationship with many large enterprises

Our production of blood collection additives, biological buffers, chromogenic substrates, chemiluminescent substrates, enzyme preparations and antigen antibodies all belong to biochemical reagents. Although we seldom hear about them, in fact, the commonly used vitamins also belong to the category of biochemical reagents. Vitamin is a kind of micro organic substance that human and animal must obtain from food to maintain normal physiological function, and it plays an important role in the process of human growth, metabolism and development. Vitamins are neither involved in the formation of human cells nor provide energy for the human body.   Vitamin A, an unsaturated monohydric alcohol, is a fat soluble vitamin, also known as anti dry eye alcohol. It is known that there are two kinds of A1 and A2. A1 exists in the liver, blood and retina of eyeball, also known as retinol. Natural vitamin A mainly exists in this form. A2 is mainly found in the liver of freshwater fish. The difference between A2 and A1 is only that there is a double bond at 3.4 of β - angelinone ring. In the blood, retinol (R) combines with retinol binding protein (RBP) and plasma prealbumin (PA) to form r-rbp-pa complex and transport it to various tissues.       There are many vitamins B, such as thiamine, riboflavin B2, niacin B3, pantothenic acid B5, pyridoxine B6, folic acid B9, cobalamin B12, inositol b-h. Vitamin C, ascorbic acid, water-soluble, mostly in fresh vegetables and fruits. Vitamin D, calciferol, also known as ossifying alcohol, anti rickets vitamin, mainly including vitamin D2, ergocalcitol and vitamin D3, cholecalciferol. This is the only vitamin that the human body can synthesize in small amounts. It mainly exists in cod liver oil, egg yolk, dairy products and yeast. Vitamin E, tocopherol fat soluble. There are mainly four kinds: α, β, γ, δ. It mainly exists in eggs, liver, fish and vegetable oil. Vitamin H, i.e. biotin or coenzyme R, is water soluble. Most of them are yeast, liver and grain. Vitamin K, naphthoquinones, fat soluble. It is a general term for a series of naphthoquinone derivatives, mainly including natural vitamin K1 from plants, vitamin K2 from animals, and synthetic vitamin K3 and vitamin K4. Also known as coagulation vitamins. It mainly exists in spinach, alfalfa, cabbage and liver. Since 2005, Desheng technology has developed and produced in vitro diagnostic reagents, such as separation gel, heparin, EDTA potassium salt, etc. it has a deep research on new Trinder's reagents, bicine, Tris and luminol, acridine ester and other biobuffers such as chromogenic substrate TOS, Maos, etc., and has a professional advantage in independent research and development and synthesis.

Today I have good news to inform you that the new blood coagulant developed and produced by desheng technology has been successfully marketed. Then why did DE sheng develop the new coagulant? Of course, it is because the new coagulant has better performance advantages compared with the original coagulant. We all know that only better products can create better services for customers.   What are the advantages of the new blood coagulant by looking at the whole coagulation experiment between the new blood coagulant and the original blood coagulant? First of all, compared with the appearance, the labeled A on the test tube is the new coagulant, while the unlabeled is the original coagulant. The appearance of the new coagulant is suspension, with less low sediment in the cup and more sediment in the bottom of the original coagulant cup. You can see the effect of spraying the coagulant evenly onto the tube wall, as shown below After even spraying, want to use hair dryer to dry first, because if alcohol does not dry, can cause hemolysis and hemolysis phenomenon. Drying after finished the vacuum tube, the next step is to blood, blood is completed, let stand at the same time, every minute picked up blood GuanPing put later to see if the blood coagulation, if after the flat, blood flow, so this time is the initial blood coagulation time, after the initial solidification, the vascular upside down, if you see a large number of serum precipitation, so this time is deep blood coagulation time, the image below for the new type of coagulant deep frozen state. You can see that the amount of serum that's coming out is quite large. It means the blood is already clotting very well. In this experiment, we found that the deep solidification time of both the new coagulant and the original coagulant was 20 minutes. All achieved a very good solidification effect. After completing all the experimental comparisons, we can clearly understand the advantages of the new coagulant. Advantages of the new coagulant: 1. The initial coagulation time of blood is relatively short, which can be done in 3 minutes 2. 10 minutes can be centrifuged on the machine, after centrifugation, the serum is very clear, no magazine, the effect is very good. 3. The application amount is very small, 20UL per pipe will not cause the spraying machine to be blocked 4. The appearance is suspension, and the cup has less low sediment after standing DE sheng technology at present, a new type of blood coagulant, has a large number of production, and now the sample has been recognised by many enterprise quality, believe that better coagulating effect, better products and services can bring more customers for a long time, in the future on the way forward DE sheng technology also will graduate students produce a better product, also warmly welcome each big enterprise business to our company on-the-spot investigation, the trial order DE sheng science and technology, a new type of coagulant products.

There are many kinds of biochemical reagents, and many of the names are similar, or there are many abbreviations of reagents which have different meanings in different industries. In addition, new beginners of procurement are very headache when looking for biochemical reagents. For example, our Trinder's chromogenic substrates are all derivatives of aniline sodium salt. Their names are very similar and their abbreviations are hard to find. At this time, we need to know the CAS number of the substance, the application and purity level of the substance. All substances have their own CAS number. CAS is the only identification code of substances in chemical biology, also known as CAS registration number or CAS registration number. It is the only digital identification number of a substance (compound, polymer material, biological sequences, mixture or alloy). In most cases, CAS can be used to identify only one substance or chemical reagent, but there are a few exceptions. Such as optically isomerized lactate dehydrogenase. CAS is not completely included, most of them are common CAS, after all, some of them have similar chemical properties and complicated distinction except for different optical rotation. Some of them are industrialized and widely used, and their physical and chemical properties are different, so they are included in different CAS numbers. Therefore, different isomers of some substances are recorded in different CAS numbers due to different optical activity, physical properties and chemical properties, while some different isomers are not distinguished by CAS numbers for the time being. Another situation is that some substances' hydrates and themselves are two different CAS numbers, but they are often mixed in daily use, which also needs attention of many companies, such as the sodium citrate and EDTA dipotassium produced by us, which are usually in the form of hydrates, and CAS numbers are mixed in use. In addition to CAS number, biochemical reagent also has purity. According to different uses, biochemical reagents usually have pharmaceutical grade, food grade, industrial grade, chemical grade, injection grade, etc.; if biochemical reagents are classified according to purity, they are industrial grade, experimental grade (LR yellow bottle label), chemical grade (CP dark blue bottle label), analytical grade (AR red bottle label), superior grade grade (GR green bottle label) and high purity (EP). The purity of industrial purity is the lowest, others are reagent purity, and the purity of high purity is far higher than that of superior purity. They are respectively used in industrial production, general chemical test, analytical test and chromatographic analysis. Since 2005, Desheng technology has developed and produced in vitro diagnostic reagents, such as separation gel, heparin, EDTA potassium salt, etc. it has a deep research on new Trinder's reagents, bicine, Tris and luminol, acridine ester and other biobuffers such as chromogenic substrate TOS, Maos, etc., and has a professional advantage in independent research and development and synthesis.

On November 21, 2019, the international medical exhibition held in Dusseldorf, Germany ended. Countless trade intentions and contracts were reached. The number of visitors also came from all over the world, which was quite diverse.   It is understood that this international medical exhibition in Germany was hosted by Dusseldorf exhibition company in Germany, and the exhibition organized by honger exhibition group and Beijing honger International Exhibition Co., Ltd. in China welcomed the participation of global medical enterprises. Including more than 5000 business units in more than 150 countries around the world, basically covering all aspects of international medical care. Although Desheng participated in the medical exhibition in the form of exhibition, it visited and communicated with global cooperative enterprises one by one.   Through this exhibition, the world is more inclined to high-end and more cutting-edge medical technology, and pays attention to quality and service. Professional technical ability is the fundamental for the survival of enterprises. Enterprises in the price war will eventually disappear in the industry competition. Desheng's visit to Germany has given us more confidence, because Desheng blood test reagent has always adhered to a high-quality, professional attitude towards the production and service of enterprises to achieve win-win results. Although the domestic medical treatment develops rapidly in recent years, there is still a certain gap from more advanced technology. This is what we need to see. We can't stop and strive for human health!

Antigens, antibodies, chromogenic or luminescent substrates and biological buffers are usually used in the in vitro diagnostic test kit, and the core of them is the various antibodies in the kit. So what are the commonly used antibodies? It is a large Y-shaped immunoglobulin secreted by plasma cells (effector B cells) and can specifically bind to antigens, which is used by the immune system to identify and neutralize foreign substances such as bacteria and viruses. According to their reaction forms, antibodies can be divided into agglutinin, sedimentin, antitoxin, lysin, opsonin, neutralizing antibody, complement binding antibody, etc. There are normal antibodies (natural antibodies) and immune antibodies (such as microbial antibodies). The function of antibody is very extensive, such as neutralizing toxin and preventing pathogen invasion, activating complement to produce membrane attacking complex to make cells dissolve and destroy, regulating phagocytosis and ADCC, mediating type I hypersensitivity reaction, crossing placenta barrier and mucosa, etc. X-ray crystallography showed that Ig consists of four polypeptide chains connected by disulfide bonds. Ig can form "Y" structure, called Ig monomer, which is the basic unit of antibody. The natural Ig molecule contains four heteropolypeptide chains, two heavy chains (H) and two light chains (L). The regions near the N-terminal amino acid sequence of Ig light chain and heavy chain are called variable region (V), accounting for 1 / 4 and 1 / 2 of heavy chain and light chain respectively; the regions near the C-terminal amino acid sequence are relatively stable, called constant region (c), accounting for 3 / 4 and 1 / 2 of heavy chain and light chain respectively. The hinge region is located between ch1 and CH2, which is rich in proline and easy to stretch and bend, thus changing the distance between antigen binding sites, which is conducive to antibody binding to antigen epitopes at different locations. The hinge region is easily hydrolyzed by papain and pepsin. Desheng technology has been developing and producing in vitro diagnostic reagents for blood detection since 2005. It has a deep research on the new Trinder's reagent, biological buffer and chemiluminescence reagent of chromogenic substrate.

Blood anticoagulants, EDTA dipotassium and EDTA Tripotassium, are common anticoagulants in blood vessels. The anticoagulant principle is to use EDTA to chelate Ca ion in blood, so as to prevent coagulation reaction and achieve anticoagulant effect. Because of the difference of one potassium atom in chemical composition, they are different in application. International Committee for hematology standardization and NCCLS (CLSI) recommend K2 EDTA as a special anticoagulant for blood cell count and volume measurement. The reasons are as follows: ● if the concentration of EDTA increases, K3 EDTA is more likely to cause erythrocyte shrinkage than K2 EDTA. (when the concentration of K3 EDTA reaches 7.5mg/ml, 11% of red blood cells will shrink.) ● if the specimen is placed for a period of time, K3 EDTA is more likely to cause the problem of cell volume increase than K2 EDTA (4 hours, 1.6% increase in volume) ● K3 EDTA is more likely to reduce the mean corpuscular volume (MCV) than K2 EDTA (usually with a negative deviation of - 0.1 to - 1.3% compared with K2 EDTA) ● if K3 EDTA is used as a liquid additive, it will cause dilution of the sample. All direct test items, such as hemoglobin, red blood cell, white blood cell and platelet count, are 1-2% lower than K2 EDTA as anticoagulant. ● using some analytical instruments, K3 EDTA will reduce the white blood cell count at a higher concentration. Brunson et al reported that the plastic K2 EDTA tube has a good consistency with K3 EDTA glass tube in the detection of cell count and classification. However, as shown before, 1-2% error due to dilution is also reported. There are differences in the pH value environments in which two and three potassium are stable. The pH of EDTA two potassium should be controlled in a weak acid environment of 2.7-6.2, the pH of its aqueous solution should be about 4.8, and the pH of EDTA three potassium should be controlled in a weak alkaline environment of 6.2-10.2, usually the pH of its aqueous solution should be about 7.5. In terms of solubility, the solubility of Tripotassium EDTA is higher than that of dipotassium, and that of dipotassium is higher than that of disodium. Since 2005, Desheng technology has developed and produced in vitro diagnostic reagents, such as separation gel, heparin, EDTA potassium salt, etc. it has a deep research on new Trinder's reagents, bicine, Tris and luminol, acridine ester and other biobuffers such as chromogenic substrate TOS, Maos, etc., and has a professional advantage in independent research and development and synthesis.  

Blood collection, you are not new. When you go to the hospital to take a blood sample, you will definitely use blood collection. The blood collection additives added to the test tubes with different color caps are different, and the blood collection vessels with different color are also different in terms of test items. What's the difference? Let's introduce the classification of blood collection vessels and the meaning of different color test tube caps. Purple cap blood vessel The anticoagulant containing EDTA-K2 in the blood vessel is usually sprayed so that the anticoagulant can be evenly distributed to the tube wall and the specimen is whole blood. This can make the blood even contact with the two potassium solution and achieve better results, which is generally suitable for routine hematological examination. Green cap blood vessel It is also called heparin tube. The blood collection vessel contains heparin sodium or heparin lithium. Heparin has the function of antithrombin directly, which can prolong the clotting time of the sample. It is generally suitable for routine biochemistry and emergency plasma detection, which has certain influence on some special biochemical items, and it is not suitable for routine hemagglutination and blood test. Heparin is a kind of mucopolysaccharide containing sulfuric acid gene, with strong negative charge, with It has the function of strengthening antithrombin to inactivate serine protease, so as to prevent the formation of thrombin, and has many anticoagulant functions such as preventing platelet aggregation. Generally, 15iu heparin can anticoagulate 1ml blood. Red cap blood vessel There is no additive in the blood collection vessel, which is suitable for routine biochemical serum test and has no effect on the test results. Disadvantages: improper centrifugation or insufficient incubation time, easy to form fibrin, easy to block the pinhole of the equipment. Collecting blood vessels with orange test tube cap The coagulant added to the blood vessels can activate the fibrin, promote the soluble fibrin to form a stable fibrin clot, and the blood collected can quickly coagulate and centrifugate out, and the results can be obtained quickly. It is generally applicable to some emergency experiments in hospitals. Through understanding, we found that the additives in the blood vessels of different color caps are different, and the corresponding examination is also different. At present, Desheng technology has successfully developed and produced a new type of coagulant and put it into the market for trial use quickly. Its setting time is faster and it can be centrifuged in 10 minutes. It meets the demand of many hospitals for quick results in emergency experiments. Desheng technology has been used as an additive for blood collection for more than ten years. In terms of quality, it can completely compete with imported enterprises. Welcome to all major enterprises Business comes to order.

First, let's understand the basic concept of anticoagulation Anticoagulation: physical or chemical methods are used to remove or inhibit some coagulation factors in the blood and prevent blood coagulation. It is called the upper pale yellow liquid after anticoagulation centrifugal separation as plasma. Anticoagulant: chemical agent or substance that can prevent blood from clotting. It is called anticoagulant or anticoagulant. Coagulation: the process of helping blood clot quickly. Coagulant: substance that helps blood clot rapidly to precipitate out of serum. The general components are gum substances. In serum test, coagulant can be selected to make blood coagulate rapidly, while in whole blood or plasma test, different anticoagulants are usually needed to make the collected venous blood not coagulate. Anticoagulants account for a large proportion in medical examination, so what are the commonly used anticoagulants? I. EDTA (ethylenediaminetetraacetate) 1. Anticoagulation principle of EDTA: it can form chelate with calcium ion in blood, so as to prevent blood coagulation. Such as EDTA-K2, etc. 2. Blood collection: Purple head tube. 3. Application: commonly used and blood routine examination, blood cell morphology examination, etc. Two, heparin 1. Common heparin products include heparin lithium and heparin sodium. Anticoagulant principle: widely exists in liver, lung and other human tissues, is a physiological anticoagulant. By strengthening the activity of antithrombin Ⅲ (at - Ⅲ), the serine protease is inactivated and the formation of thrombin is prevented, so as to play an anticoagulant role. Such as heparin lithium, heparin sodium, etc. 2. Blood collection: green head tube. 3. Application: it is commonly used in biochemical tests. III. oxalate 1. Anticoagulation principle: oxalate reacts with calcium ion to form calcium oxalate precipitate, so as to prevent blood coagulation. Such as sodium oxalate, ammonium oxalate, etc. 2. Blood collection: gray head tube. 3. Application: it is often mixed with sodium fluoride (which can inhibit glucose fermentation) for blood glucose determination. Since 2005, Desheng technology has developed and produced in vitro diagnostic reagents, such as separation gel, heparin, EDTA potassium salt, etc. it has a deep research on new Trinder's reagents, bicine, Tris and luminol, acridine ester and other biobuffers such as chromogenic substrate TOS, Maos, etc., and has a professional advantage in independent research and development and synthesis.

The market share of in vitro diagnostic reagents in China can reach about 70%. Domestic in vitro diagnostic reagents are relatively mature. After years of development, China's independent innovation ability in the field of in vitro diagnostic reagents has been significantly improved, and the overall technical level has basically reached the international level at the same time. There are more than 200 kinds of in vitro diagnostic reagents. The main products on the market are liver function, kidney function, blood lipid, special protein, etc.                                                             Clinical biochemistry is based on the normal biochemistry of human body to study the biochemical changes in pathological state. By analyzing the changes of related metabolites, we can find the characteristic markers and establish the corresponding detection methods, so as to provide biochemical information and decision-making basis for disease prevention, diagnosis, treatment and prognosis. In the application of in vitro diagnosis, biochemical diagnosis is a commonly used diagnostic method. Biochemical diagnosis refers to the in vitro diagnostic method which uses the Lamber Beer law to determine various inorganic elements, protein and non protein nitrogen, enzyme, sugar, lipid and other biochemical indexes in vitro through various biochemical reactions. Our commonly used inspection items of liver function, renal function, blood glucose, blood lipid and so on belong to Biochemical diagnosis. Clinical biochemical diagnostic reagents are used in combination with biochemical analyzer to diagnose the relevant clinical biochemical indexes of human body by applying the principles of chemistry, enzymology, immunology and other related technologies. Through the specific reaction between reagents and related substances to be tested, specific optical signals are given, recorded by biochemical instrument, and compared with calibrator to give the level of related substances to be tested. Biochemical diagnosis is one of the most commonly used in vitro diagnosis methods, and it is also the earliest and most mature IVD subdivision field at home and abroad. In the 1990s, a large number of in vitro diagnostic reagent manufacturers and import agents have sprung up. With the increase of production enterprises and the fierce increase of the demand for commercial reagents in clinical tests, Desheng technology in vitro diagnostic business has also got the opportunity of rapid development, rapid growth and rapid development. Biochemical production line is increasingly rich and mature. In the 21st century, China's in vitro diagnostic reagent industry has entered a mature stage. In today's biochemical reagent production enterprises, a large number of national and excellent enterprises that can be compared with international famous manufacturers emerge, such as: Wuhan Desheng, Beijing Jiuqiang, Beijing Lidman, Shanghai Kehua, Ningbo Meikang, etc. In recent decades, the biochemical diagnosis industry in China has started from scratch and is growing and improving. At present, it has gone out of the country to the world.

Anticoagulation mechanism of EDTA-K2   EDTA-K2 is one of the most commonly used and important anticoagulants and reagents in the clinical work. Its mechanism is to prevent blood coagulation by forming stable chelates with calcium ions in the water phase. The salts of EDTA include potassium, sodium, lithium salt, which are all soluble in water. The solubility of potassium salt is higher than that of sodium salt. It is better to use potassium salt of EDTA for whole blood cell count. EDTA can affect the activity of some enzymes and inhibit lupus erythematosus factor, so it is not suitable for making histochemical staining and blood smear to examine lupus erythematosus cells. EDTA can also affect platelet aggregation and leukocyte phagocytosis, and is not suitable for hemostatic test and platelet function test. The smear prepared with EDTA-K2 anticoagulant has an effect on the morphology of granulocytes, the degree of which is related to the time of anticoagulant placement and the concentration of   anticoagulant.                                                                             Anticoagulation mechanism of heparin sodium Heparin is the best anticoagulant in the determination of blood chemical composition. Heparin is a kind of mucopolysaccharide with sulphuric acid group, with molecular weight of 15000. Its anticoagulation mechanism is to work with anticoagulant II to inhibit the action of factors ⅸ a, Ⅷ and PF3 in low concentration, and to strengthen the action of anticoagulant III to inactivate serine protease, so as to prevent the formation of thrombin; it also has the effect of inhibiting the self catalysis of thrombin and inhibiting factor X. The salts of heparin are sodium, lithium and ammonium. Generally, the anticoagulant dosage of heparin is 10.0-12.5iu/ml blood. Although the price of heparin lithium is expensive, its anticoagulation effect is better. Because heparin sodium can increase the content of plasma sodium, while heparin ammonium can increase the content of urea ammonia. There are obvious differences in some biochemical components between serum and heparin anticoagulant plasma. In the process of coagulation, the content of serum potassium is higher than that of plasma due to the dissolution and destruction of red blood cells. Therefore, when determining potassium ion, pay attention to the difference between serum and plasma. In addition, heparin excess can cause leukocyte aggregation and thrombocytopenia, so it is not suitable for leukocyte classification and platelet count, nor for hemostatic test. The results showed that heparin sodium anticoagulant had a significant effect on the determination of total protein in blood, which was 3% - 5% higher than that in serum. However, after adding anticoagulant, the total protein content is lower than the total protein content of serum. The reason is that after adding heparin sodium anticoagulant to blood, the production of thrombin is blocked, the blood coagulation is prevented, the fibrinogen cannot be hydrolyzed into fibrin monomer, and the formation of fibrin monomer is effectively blocked, and these fibrin remains in plasma. Serum is the blood after coagulation, fibrinogen hydrolysis into fibrin monomer, and because of coagulation and consumption, after centrifugation with the blood cells separated. Therefore, the total protein quality in plasma is all the proteins including fibrinogen, while the total protein content in serum is no fibrinogen. Theoretically, it should be lower than the total protein content in plasma. Therefore, when determining the total protein content of blood, it's better to take serum as the sample. For some emergency samples, or some patients who need to be determined urgently for other reasons, when it's necessary to use plasma as the sample to measure the total protein, after measuring the total protein, the content of fibrinogen should be deducted (generally 3% - 5%), so as to be consistent with the total protein content measured by taking serum as the sample, It truly reflects the actual total protein content in patients. The quality of EDTA 2k and heparin sodium produced by Desheng technology can fully compete with the imported enterprises. Our products have established long-term cooperative relations with many enterprises. Some customers order 100 kg of EDTA 2kg of potassium in Desheng technology every month. We believe that good products and good services will surely bring more long-term customers, and that customer-oriented, symbiotic and win-win situation is the common goal of Desheng and all major enterprises.