China Wuhan Desheng Biochemical Technology Co., Ltd Company News

    The chromogenic substrate TOPS, the full name of N-ethyl-N- (3-sulfopropyl) -3-methylaniline sodium salt, is similar to TOOS, the difference in molecular structure is that the sulfopropyl group on the single atom connected to the benzene ring With a hydroxyl group, the stability is slightly stronger, and all have the characteristics of high water solubility and high detection sensitivity. Like TOOS, TOPS can also be used for uric acid detection in kidney function kits. The chromogen used by uric acid detection kits of different companies is slightly different (the chromogenic substrate ESPMT used by some companies refers to TOPS). In addition, TOPS is also commonly used for liver function series free fatty acid detection and renal function series creatinine detection. The following is an example of the principle of uric acid detection.        During clinical testing, first collect venous blood from the test subject. The subject should be fasting for 12 hours. It is best not to drink alcohol in the morning and 12 hours. The blood collection tube should be disinfected. The blood sample is not hemolytic. The detection of uric acid is to first catalyze the complete oxidation of uric acid with oxygen and water to allantoin, carbon dioxide and hydrogen peroxide H2O2. H2O2 reacts with TOPS and 4-AAP under the catalysis of POD. TOPS is oxidized into a chromogenic product, and the absorbance is measured with a spectrophotometer. The intensity of the absorbance is proportional to the uric acid content, so that uric acid analysis is performed. This is a typical biochemical detection method using two-step enzymatic reaction to measure.         Desheng Technology has more than ten years of experience in R&D, production and sales in the field of diagnostic raw materials, especially in the development and improvement of new Trinder's reagents in the aniline sodium salt derivative series. It performs functional groups on the basis of traditional chromogenic substrates. Replacement of the increase and decrease, continue to make optimization and performance improvement for the needs of clinical testing!

     The full name of TOPS (CAS No.:40567-80-4) is N-ethyl-N-(3-sulfopropyl) -3-methylaniline sodium salt, which is a white to light brown powder, also a member of the new Trinder's reagent family. Maybe you think the name sounds humongous, and it is a vocabulary that a fully professional pharmacist only understands. It is even more difficult to relate to your life. If you think so, you are wrong. You may not have heard of this name, but I think most of you or people around you have done biochemical tests in the hospital, that is, liver function, kidney function, uric acid, cholesterol and other tests. Most of these tests are carried out by automatic biochemical analyzers, uric acid analyzers, etc. in conjunction with the corresponding kits. And one of the core raw materials in these kits is the TOPS we talk about today.        In humans and primates, uric acid is the final product of purine metabolism. It is produced by the oxidation of xanthine and hypoxanthine by xanthine oxidase and is excreted in the urine. High serum uric acid and hyperuricemia are associated with insulin resistance, cardiovascular disease and gout. The mechanism leading to hyperuricemia is usually increased uric acid production or decreased urine excretion. Increased serum uric acid may be a sign of kidney disease, so the early diagnosis of kidney disease can be indirectly through the test of uric acid.   TOPS Product description Chinses name N-ethyl-N- (3-sulfopropyl) -3-methylaniline sodium salt English name Sodium 3-(N-ethyl-3-methylanilino)propanesulfonate CAS No. 40567-80-4 Hs code 2922199090 Molecular formula C12H18NNaO3S, H2O Molecular weight 297.34 Appearance White or light brown powder Storage condition 0-5℃，away from light and moisture Transport condition Room temperature（20-25℃） Maximum absorption wavelength 550 nm Oxidation reaction Applacation This product is used for the colorimetric determination of uric acid and cholesterol. It has the characteristics of good water solubility, high sensitivity and strong stability. Cholesterol colorimetric determination; water-soluble reagent, used for catalase photometric determination.         In the presence of hydrogen peroxide and peroxidase, TOPS reagent is formed during the oxidative coupling reaction with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazolone hydrazone (MBTH) Very stable purple or blue dye. The substrate is enzymatically oxidized by its oxidase to produce hydrogen peroxide. The concentration of hydrogen peroxide corresponds to the substrate concentration. Therefore, the amount of the substrate can be determined by the color development of the oxidative coupling reaction to obtain a relatively accurate test result of the concentration of the analyte. TOPS produced by Desheng has obtained the quality certification of more than 100 manufacturers nationwide. Because of its color rendering sensitivity, color stability and anti-interference, as well as the accuracy and precision of specific measurement items, it has been well received by customers. You are welcome to inquire, the company will provide you with quality products and excellent services, so that your products rank among the industry's leading level! Company website: https://www.vacutaineradditives.com, Phone No.: +86-711-3702650.

The full name of the chromogenic substrate MAOS is N-ethyl-N- (2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt monohydrate, CAS No. is 82692-97-5. As other new Trinder ’s reagents, it has high water solubility and sensitivity much higher than traditional chromogenic reagents commonly used in some laboratories, so it is widely used in clinical testing and biochemical reagent kits.   First, the biggest feature of MAOS is that the maximum absorption wavelength of its oxidized product is far more than that of ordinary chromogenic reagents. Even in the new Trinder ’s reagent, its product UV absorption wavelength is 630nm, which is quite high. The maximum absorption wavelength of products of many chromogenic reagents is in the visible light region. If testing human blood or other body fluids, due to the complex components in the sample to be tested, the sample itself contains a small amount of components that absorb wavelengths in the visible light region or absorb with chromogenic products. The wavelengths are close, which will make the detection result high. If you use something like MAOS, the maximum absorption wavelength of the product is in the ultraviolet region and it is relatively high, which will greatly reduce interference. Therefore, it is recommended to use MAOS as a chromogenic substrate for some biochemical testing items that require highly accurate values.   Secondly, the chromogenic reaction of MAOS chromogenic substrate has a wider adaptability to the pH of the reaction system, which greatly improves its adaptability. The reaction of some chromogenic reagents is often performed under acidic conditions, but the biochemical detection usually requires the participation of enzymes. We know that the catalytic effect of enzymes is relatively sensitive to the pH of the reaction environment, and it may not match the chromogenic reagent Inactivation will be greatly restricted in use, and MAOS reagents do not have this problem.   Another point is that the MAOS chromogenic reaction has a larger molar absorption intensity, so its sensitivity is relatively high. It should be noted that MAOS will fade when the detection time is too long, so the MAOS detection needs to be completed in time and cannot be interrupted. In addition, MAOS is also relatively safe. Unlike DAB and other biphenyl chromogenic reagents, although the price is relatively low, it has certain carcinogenicity or mutagenicity.   Desheng Technology has been committed to the development, production and sales of various chromogenic substrates, especially TOOS, MAOS, ADPS, etc. have a good reputation at home and abroad, and have good cooperation with many biochemical kit manufacturers Relationship, and jointly contribute to the field of domestic diagnostic reagents!

As a new Trinder's reagent, MAOS occupies a pivotal position in it. It is a white powder that is easily soluble in water. Chinese name: N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3 , 5-Dimethylaniline sodium salt monohydrate, sensitive to light and humidity, CAS number: 82692-97-5, purity requirement> 99%, molecular structure is shown in the following figure:   Application of MAOS The new Trinder's reagent is a highly water-soluble aniline derivative, which is widely used in diagnostic tests and biochemical tests. It has several advantages over conventional color-generating reagents in the colorimetric determination of hydrogen peroxide activity. The new Trinder's reagent is stable enough to be used in both solutions and test line inspection systems. In the presence of hydrogen peroxide and peroxidase, the new Trinder's reagent during the oxidative coupling reaction with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazole sulfone hydrazone (MBTH), Forms a very stable purple or blue dye. The molar absorbance of the coupling dye with MBTH is 1.5-2 times higher than that with 4-AA; however, the 4-AA solution is more stable than the MBTH solution. The substrate is enzymatically oxidized by its oxidase to produce hydrogen peroxide. The concentration of hydrogen peroxide corresponds to the substrate concentration. Therefore, the amount of substrate can be determined by the color development of the oxidative coupling reaction. Glucose, alcohol, acyl-CoA and cholesterol can be used to detect those substrates coupled with the new Trinder's reagent and 4-AA. There are 10 new Trinder's reagents, and the use of MAOS members is also very important. For specific substrates, testing different types of new Trinder's reagents is necessary to develop the best detection system.   Product introduction Oxidizing chromogen reagents, high water solubility, stable aniline analogues, a wide pH range for color development and oxidation reactions   Preparation of detection solution-MAOS (other substrate configuration methods are basically the same) 1. Dissolve 20 mg MAOS in 10 ml PBS to prepare a 6.6 mM MAOS solution. (The specific solubility is adjusted as needed) 2. Dissolve 14 mg 4-aminoantipyrine (4-AA) in 10 ml PBS to prepare a 6.6 mM 4-AA solution. 3. Prepare 2 U / ml horseradish peroxidase solution in PBS. 4. Mix equal volumes of each solution to prepare a test solution. Store the test solution in the dark at 4 ℃   Detection steps 1. Prepare a sample solution for the enzymatic oxidation reaction. The pH value of the buffer solution should be 5.5-9.5. 2. Use the same buffer to prepare a standard solution containing a known amount of substrate. 3. Add appropriate units of oxidase to the sample solution, followed by an equal volume of detection solution. 4. Incubate the mixture at room temperature or 37 ° C for 30 minutes to 1 hour. 5. Determine the O.D. value at 555 nm. 6. Prepare a standard curve and determine the substrate concentration in the sample solution     MAOS belongs to one of Desheng Biochemical's key sales varieties. At the same time, TOOS, TOPS, ADOS, ADPS, ALPS, DAOS, HDAOS, MADB and other new Trinder's reagents are available for customers to choose. Desheng always upholds: Honesty first; quality first, technology leading; customer-oriented, symbiosis and win-win; pioneering and innovative, the pursuit of excellence in business philosophy, dedicated to providing our customers with quality products and services, welcome to consult and cooperate!  

TOOS is also called EHSPT (N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt), which is a highly water-soluble sodium salt of aniline. It is sensitive when performing Trinder reaction. It is much higher than traditional phenol, aniline, ADA and other color-developing substrates. Clinical biochemical testing is often used in liver function kits, blood glucose metabolism kits, etc. In the presence of hydrogen peroxide and peroxidase POD, Trinder's reagent forms a very stable orange or red quinone with 4-aminoantipyrine (4-AAP). The molar absorbance of TOOS and MBTH coupling dye is 1.5-2 times higher than that of 4-AA coupling dye; however, 4-AAP solution is more stable than MBTH solution, so Trinder ’s reagent is more used with 4-AAP. When tested with a biochemical kit, the measured indicators such as uric acid, glucose, glycated albumin, and 1,5-anhydroglucitol are equal to the enzymatic oxidation of its oxidase to produce hydrogen peroxide. The concentration of hydrogen peroxide corresponds to the concentration of the substance to be tested Therefore, the amount of the analyte index can be determined by the color development of the oxidative coupling reaction. Certainly, the indicators of enzyme activity can also be measured, such as the adenosine dehydrogenase detection kit and 5'-nucleotidase detection in the liver function series, which is the reaction of the enzyme to be detected and its corresponding catalytic substance to generate peroxide Hydrogen is then reacted with the generated hydrogen peroxide through the coloring substrate TOOS coupling 4-AAP, and the final coloring product production rate corresponds to the activity of the tested enzyme, thereby achieving the purpose of measuring the index. The TOOS color development substrate developed by Desheng Technology has the characteristics of high purity, high water solubility, and low interference ion. It is very convenient to use when it is used, and the company also produces Tris buffer used with it for biochemical detection. Wait, the quality and service are well received!

I believe that everyone must have had such experience. When going to the hospital for medical treatment or routine medical examination, sometimes the doctor will ask you to do a blood test and a complete biochemical examination. In fact, the clinical biochemical detection mentioned here refers to studying the biochemical changes under the pathological state on the basis of the normal metabolism of the human body, by analyzing the changes of related metabolites in the body, looking for characteristic markers, establishing corresponding detection methods for disease prevention , Diagnosis, treatment and prognosis to provide biochemical information and decision-making basis. Furthermore, biochemical diagnosis refers to an in vitro diagnostic method that uses Lamber-Beer's law to measure various biochemical indexes of various inorganic elements, proteins, and non-protein nitrogen and enzymes, sugar, and lipids in vitro through various biochemical reactions. Liver function, kidney function, blood sugar, blood lipids and other inspection items belong to the biochemical diagnosis. Clinical biochemical diagnostic reagents and biochemical analyzers are used in combination, and the relevant technical principles of chemistry, enzymes, immunology, etc. are applied to diagnose clinical biochemical indicators related to the human body. Through the specific reaction between the reagent and the related analyte, a specific optical signal is given, which is recorded by the biochemical analyzer and compared with the calibrator to give the level of the related analyte. Biochemical diagnosis is one of the most commonly used in vitro diagnostic methods at present, and it is also the earliest and most mature IVD subdivision field developed at home and abroad. Among them, Trinder's reaction is a routine biochemical detection project catalyzed by a specific substrate oxidase. The oxidase catalyzes the substrate to produce hydrogen peroxide (H202). In the presence of phenol and 4-aminoantipyrine, hydrogen peroxide Peroxidase catalyzes the production of quinone compounds and water, which reflects the amount of hydrogen peroxide produced by the coloration of the quinone compounds, and indirectly detects a certain substrate concentration or a certain oxidase activity. Among the new Trinder's reagents, TOOS is the most commonly used because of its high water solubility, high molar absorbance (that is, more sensitive color reaction), color reaction intensity and fading. TOOS produced by Desheng is favored by more than 100 manufacturers because of its crystal morphology, high crystal purity, and pure white crystal color (important for this color reaction). The following are the relevant indicators of TOOS, please refer to it. TOOS   Product description TOOS is a new type of high water-soluble aniline derivative, widely used in diagnostic tests and biochemical tests. molecular weight 295.33 Molecular forluma C12H18NNaO4S Chemical structure CAS No. 82692-93-1 purity ≥99.5% SMILES O=S(CC(O)CN(CC)C1=CC=CC(C)=C1)([O-])=O.[Na+] Packaging details 10g/bottle；50g/bottle Transpotation conditions Room temperature（20℃-25℃） Storage conditions powder -20°C 3 years 4°C 2 years solution -80°C 6 months -20°C 1 month   remarks In Vitro: DMSO : ≥ 47 mg/mL (159.14 mM) *"≥"means soluble and does not represent saturation.   As a domestic senior manufacturer specializing in blood test reagents for 15 years, Desheng is committed to providing raw materials to help accurate diagnosis and treatment. Its TOOS products have been evaluated by more than 100 professional manufacturers in the industry and received numerous praises. If you are interested, please call to consult!

Many customers who purchase lithium heparin are facing a problem. They inquire from many lithium heparin manufacturers. In order to reduce procurement costs, they often choose the lower price manufacturer. After receiving the goods, they find that the product quality is not guaranteed at all. Must look at the cost performance. Buying products that do not meet the test requirements at a low price is a waste of manpower, material resources, and financial resources. Wise people know that it is impossible to buy really good products at a low price. The heparin produced by Desheng Lithium is a very reliable choice. Lithium heparin not only has a variety of potency for its choice, but the price is also worthy of that quality. Really achieve high efficiency and price ratio, high quality and high guarantee. Desheng always believes that quality is the prerequisite for everything. Only good products can be repeatedly purchased by customers. Some issues that need to be paid attention to when purchasing lithium heparin, 1. In order to ensure adequate blood anticoagulation, the heparin lithium anticoagulant test tube must be reversed and mixed 5-8 times as soon as possible after blood collection, especially when the blood collection temperature is higher than 25 ℃, the blood and lithium heparin must be mixed in time and Otherwise, it may easily lead to blood clotting or local clotting. 2. Lithium heparin anticoagulation is not irreversible anticoagulation, so the test should be completed within 6 hours after the blood collection of the heparin lithium anticoagulation test tube, otherwise it may cause errors in the test results. The dosage of lithium heparin should ensure that the specimen is fully and partially anticoagulated, so that most of the plasma indicators can be repeated within 6 hours, especially sensitive indicators such as AST, ALT, TBIL, DBIL, and GGT. 3. Lithium heparin can be used together with the separation gel. The anticoagulation effect, tube wall siliconization, centrifugal conditions, separation gel quality, etc. will affect the blood separation effect. It is recommended to use with the blood separation gel produced by our company to obtain high-quality plasma specimens. Recommendation for irradiation sterilization after adding to blood collection tube: use γ-ray irradiation, the dose is 8-25kGy. The irradiation dose can be determined by the initial colony number. The lithium heparin product developed by Desheng has the characteristics of customizable potency. The conventional one is 160IU / mg, but it can be adjusted according to customer needs. 150IU / mg, 170IU / mg, 180IU / mg, the higher the potency, the price It will also change with it. Desheng's lithium heparin product has higher purity than other companies, and the appearance is pure white. It can be seen directly by the naked eye that there are no foreign substances in other magazines, and the price is also higher than other products. Manufacturers are more favorable, and products are sold all over the country. Desheng is the first choice for purchasing lithium heparin products.    

Heparin is a blood anticoagulant commonly used in vacuum blood collection tube. Heparin anticoagulant is usually its sodium, potassium, lithium, ammonium salts, such as sodium heparin, lithium heparin, etc., among which lithium heparin is the best. The appearance of the finished lithium heparin is white to almost white powder, odorless, soluble in water and hygroscopic. According to the study, there was no significant difference in the results of TP, ASO, UA, ALT, Mg, Cl, TC and CRP between lithium heparin anticoagulant plasma and serum (P > 0.05). There were significant differences in the results of HBD, LDH and TBA between lithium heparin anticoagulant plasma and serum (P < 0.05). Therefore, except for HBD, LDH and TBA, the correlation between lithium heparin anticoagulant plasma and serum is good. Therefore, it is feasible to replace serum with lithium heparin anticoagulant plasma in life detection, which can be used as an important detection method. [Scope of Application] 1. The product is suitable for blood sample collection and anticoagulation in clinical biochemical examination and emergency biochemical examination, and also for blood sample collection and anticoagulation in some hemorheology projects. 2. Lithium heparin is recommended as an anticoagulant when determining ionic content in blood in clinical tests, because it is least likely to interfere with the determination of other ions. 3. This product is not a drug and cannot be used as an injection. Direct injection into human body and animals is prohibited. [Precautions] Heparin salt test tube must be inverted and mixed 5-8 times as soon as possible after blood collection. Heparin anticoagulation is not irreversible anticoagulation, so the lithium heparin test tube should be completed within 6 hours after blood collection. 3. Heparin may be involved in the metabolism of cellular enzymes and ions, and the amount of heparin additive should ensure adequate anticoagulation of all and local specimens, especially sensitive indicators such as AST, ALT, TBIL, DBIL and GGT. 4. Lithium heparin can be used with separation gel at the same time. Anticoagulation effect, wall silicification, centrifugation conditions, separation gel quality, etc. will affect the blood separation effect. It is suggested to cooperate with the serum separation gel produced by our company. 5. Suggestions on Sterilization: use γ irradiation at a dose of 8-25 kGy. De Sheng's lithium heparin potency is ≥ 150 IU/mg and anhydrous potency is≥160 IU/mg) are controlled with reference to the standard of sodium heparin API. The lithium heparin aqueous solution can be sealed and stored at 0-4℃ in a sterile state, and should be disposed and used up as soon as possible. Its maximum shelf life should not exceed 7 days,  

[Product name]Virus Transport Media [Packaging specifications]1L/bottle、5L/bottle [Product performance] Non-inactivated type: it does not contain lysate, which can maintain the activity and integrity of pathogen, and can be used for virus culture and isolation. Inactivated type: it can split pathogen instantly and release nucleic acid, and the protective agent can prevent nucleic acid from degradation. [Scope of use] The Virus Transport Media can be used for the collection, preservation and transportation of nasopharyngeal pathogen specimens such as new coronavirus, influenza, bird flu, hand-foot-mouth disease, measles and so on. Non-inactivated type: On the basis of Hank's, BSA (bovine serum albumin) amino acids, vitamins and other nutrients are added to maintain the virus' activity over a wide temperature range and maintain the originality of the sample to the greatest extent and can be used for virus nucleic acid extraction and detection, virus culture and isolation. Inactivated type: By fully mixing the collected samples with virus lysate and virus nucleic acid preservation solution, the virus in the samples can be inactivated, and the integrity of virus nucleic acid in the samples can be effectively guaranteed. The collected samples can be transported and preserved for a long time under normal temperature. The preserved viral RNA samples can be widely used for genetic testing, enzyme-linked immunoassay (ELISA), PCR testing, ect. [Storage conditions and time] Non-inactivated type: Store at 4-25 ℃. It shall be transported to the laboratory within 48 hours under 2-8 ℃ after sampling, or stored under - 70 ℃. Inactivated type: 30 days at normal temperature

In biochemical experiments or tests, almost all reactions need to be controlled within a certain pH range, especially those involving enzymes. PH changes have a greater impact on the reaction, so biological buffers are used to maintain the pH value of the reaction environment. In biochemical experiments, many of them simulate the detection reaction in the organism, which also requires high pH. Biological body is regulated by humoral fluid, so biological buffer is needed in the experiment. There are many types of biological buffer, and the preparation methods are somewhat different, but the principle is generally the same. Sodium hydroxide is usually added to the conjugate acid or hydrochloric acid is added to the conjugate base. Both methods can form a buffer solution with sufficient concentration of conjugate acid-base pairs. Here is the preparation method of a small amount (about 1-2l) of dihydroxyethylglycine Bicine or hydroxyethylpiperazine propionic acid EPPS:   Preparation of Bicine buffer   (1) 0.1M solution (A): Bicine 16.317g/ deionized water 1,000ml   (2) 0.1m NaOH solution (B):NaOH 4g/ deionized water 1,000ml   (3) the pH 5.1 1000 ml + 0 ml (B) (A) (A) : (B) = 5-0   PH 7.8 + 200 ml 1000 ml (A) (B) (A) : (B) = 5 to 1   PH 8.2 + 400 ml 1000 ml (A) (B) (A) : (B) = 5-2   PH 8.6 + 600 ml 1000 ml (A) (B) (A) : (B) = o   PH 10.4 + 800 ml 1000 ml (A) (B) (A) : (B) = five   Preparation of EPPS buffer   (1) 0.1M solution (A): EPPS 25.233g/ deionized water 1,000ml   (2) 0.1m NaOH solution (B):NaOH 4g/ deionized water 1,000ml   (3) the pH 5.2 1000 ml + 0 ml (B) (A) (A) : (B) = 5-0   PH 7.3 + 200 ml 1000 ml (A) (B) (A) : (B) = 5 to 1   PH 7.8 + 400 ml 1000 ml (A) (B) (A) : (B) = 5-2   PH 8.2 + 600 ml 1000 ml (A) (B) (A) : (B) = o   PH 8.8 + 800 ml 1000 ml (A) (B) (A) : (B) = five     It should be noted that: the preparation temperature is controlled at 20 degrees. If accurate pH value is needed, it can be measured with A pH meter, and then the proportion of solution A and solution B can be adjusted to A specific pH value. If it is necessary to exclude sodium ions in the test environment, it is also possible to replace sodium hydroxide with potassium hydroxide and change the weight according to the corresponding molecular weight.   DE sheng technology since the 05 years began to research and development production of separation, heparin, EDTA potash mining vessel additives, and in vitro diagnostic reagent, the color of the original substrate TOOS, MAOS new Trinder 's reagent, Bicine and Tris biological buffer and luminol, acridine esters such as chemiluminescence reagent has a deep research, in the independent research and development and synthesis of professional advantage.

  A lot of people know that the blood coagulant we produce is a suspending agent, which needs to be shaken before use. Is this of poor quality or can it not be shaken? The answer is: it needs to be shaken. The coagulant is different from our anticoagulants, such as EDTA dipotassium and heparin. The specific need is from its state characteristics. Different from the anticoagulants such as EDTA dipotassium, heparin and sodium citrate, the coagulant is not a solution, but a suspension, also known as a suspension. The reason why the anticoagulant is a solution state is that EDTA dipotassium and heparin are soluble in water, which exists in the size of molecules or ions in the water, and some components of the coagulant are insoluble in water, which is stored in small solid particles Yes. This is very similar to the muddy lime water, because the calcium hydroxide is slightly soluble in the water, a large number of small solid particles of calcium hydroxide are suspended in the water, forming a suspension.   Solution or suspension is essentially the dispersion system of mixture, and the dispersion phase is dispersed in the dispersion medium. Dispersion system is often called uniform single-phase solution, heterogeneous multi-phase colloid and coarse dispersion system suspension and emulsion with particle size more than one micron. Emulsion is similar to suspension except that the dispersed phase is liquid. Therefore, all suspensions are of large particle size. The coagulant components of the dispersed phase are insoluble in the dispersing medium such as alcohol, but for the naked eye, the particles are relatively small, and precipitation or stratification will occur after placement. Under the condition of carbon dioxide isolation, the turbid lime water will be slowly clarified after a long time of standing. Then our coagulant suspension will be gradually clarified from the upper layer after standing for a long time, so it is necessary to shake it well before use. It is worth noting that although the problem of long-term sedimentation of blood coagulant suspension is not the main quality problem of coagulant, but for the convenience of use, there are still differences. The coagulant newly developed by Desheng has made a great improvement in this aspect. Compared with the previous coagulant, in addition to the improvement of blood coagulation effect and speed, it is smaller in particle size, although long-term sedimentation is also possible, However, the time of long-term precipitation is greatly prolonged, and the suspension can be shaken into uniform suspension only by slight shaking.

There are two kinds of blood anticoagulant heparin salt produced by us, namely heparin sodium and heparin lithium. This is because heparin lithium is made from heparin sodium, which can only be worthwhile by conducting cation exchange with heparin sodium through ion exchange resin. Here is a brief introduction to the preparation process and the main equipment used. The preparation of heparin lithium in foreign countries is usually made with high-quality heparin sodium as the raw material, which has a high raw material cost, and the ion exchange link is carried out under the condition of strong acid pH1.5 and low temperature, resulting in high titer loss, low yield and high production cost. In China, the preparation of heparin lithium has been greatly improved according to the above problems. The crude heparin sodium is directly used as the raw material, and after enzymatic hydrolysis by heparin enzyme, deproteinization, decolorization by hydrogen peroxide oxidation, and multiple alcohol-precipitation steps, lithium chloride is added to conduct cation exchange through cation exchange resin, which is worthy of high-quality heparin lithium. This method greatly saves the cost of raw materials and production costs. In the preparation of heparin lithium, the exchange of sodium ions into lithium ions by cation exchange resin is the core step. Ion-exchange resin refers to the polymer compound with the active group that can adsorb ions, which has network structure and is insoluble. Its full name consists of classification name, skeleton name and basic name. Pore structure is divided into gel type and large pore type, properties are divided into strong acid cationic resin, weak acid cationic resin, strong basic anion resin, weak basic anion resin, in addition to the basic type of chelation, amphoteric, oxidation reducibility. Resin matrix manufacturing raw materials are mainly styrene and acrylic acid and divinylbenzene polymerization resin of two categories. In the use of reagents, sometimes do not use cation or anion to exchange resin hydrogen ions or hydroxide ions, grams to avoid the solution pH changes caused by the impact. For example, in the preparation of heparin lithium, lithium chloride is now used to exchange the hydrogen in the ion exchange resin, and the lithium ion resin is used to exchange the sodium in the heparin sodium, so that the heparin lithium is worth; Resin is regenerated not by acid, but by lithium chloride. Different resins have different adsorption rules for ions. Generally speaking, high-priced ions are adsorbed by some, while those with large diameter of isovalent ions are preferentially adsorbed. DE sheng technology since the 05 years began to research and development production of separation, heparin, EDTA potash mining vessel additives, and in vitro diagnostic reagent, the color of the original substrate TOOS, MAOS new Trinder 's reagent, Bicine and Tris biological buffer and luminol, acridine esters such as chemiluminescence reagent has a deep research, in the independent research and development and synthesis of professional advantage.