ADA Biological Buffer Product Introduction

1,Product Overview

ADA (N (2-acetamido) iminodiacetic acid) is a zwitterionic biological buffer widely used in the fields of biochemistry and molecular biology. The CAS number is 26239-55-4, the molecular formula is C ₆ H ₁₀ N ₂ O ₅, and the molecular weight is 190.15 g/mol. This product is a white crystalline powder with a purity of over 99%, soluble in water and ethanol.

2,Core Features and Advantages

1. Suitable buffer range

The effective buffering range of ADA is pH 6.0-7.2, with a pKa value of approximately 6.6 at 25 ℃. This range covers the optimal pH range for numerous enzymatic reactions and biological processes, enabling it to stably maintain the acid-base environment of the reaction system in various experimental scenarios.

2. Characteristics of zwitterionic substances

ADA has a zwitterionic structure, carrying both positive and negative charges, which enables it to effectively resist pH changes caused by external acids or bases. Meanwhile, the zwitterionic properties endow ADA with the advantage of low cell membrane permeability, reducing interference with normal cellular metabolism.

3. Metal ion chelating ability

ADA has excellent metal ion chelating properties and can chelate metal ions such as manganese (II), copper (II), nickel (II), zinc (II), cobalt (II) in a ratio of 2:1. This characteristic makes it uniquely valuable in experimental systems that require control of metal ion concentration.

4. Prevent protein oxidative denaturation

In gel electrophoresis and other experimental processes, ADA can effectively prevent protein from being oxidized and irreversible denaturation, and ensure the accuracy and reliability of experimental results.

5. Low UV absorption

ADA has low UV absorption at wavelengths below 260nm, which makes it suitable for analytical experiments that require UV detection, avoiding interference from the buffer itself on the detection signal.

6. Low temperature coefficient

The pKa temperature coefficient of ADA (Δ pKa/° C=0.011) is relatively low, which means that its pKa value fluctuates less during temperature changes, ensuring the stability of pH value during transportation, storage, and use.

3,Main application areas

1. In vitro diagnostic reagents

ADA buffer has been widely used in biochemical diagnostic kits, DNA/RNA extraction kits, and PCR diagnostic kits. In ELISA (enzyme-linked immunosorbent assay), ADA buffer can be used as coating buffer, washing buffer, and sample/conjugate diluent. In lateral chromatography detection, ADA buffer can be used as a key component for the preparation of running buffer.

2. Cell culture

ADA can be added to protein free culture medium to support cell growth. ADA has been validated as an effective pH stabilization tool in mammalian cell culture. ADA exhibits low toxicity to cells at conventional working concentrations.

3. Protein research and electrophoresis

ADA is suitable for preparing solid phase pH gradient, which can prevent protein from being oxidized and irreversible denatured during gel electrophoresis. Its low UV absorption characteristics also make ADA an ideal choice for technologies such as protein crystallization, isoelectric focusing, and capillary electrophoresis.

4. Enzyme reaction system

In enzymatic reactions, ADA can provide a stable reaction environment for enzymes in the weakly acidic to neutral pH range, ensuring enzyme activity and reaction reproducibility.

5. Pharmaceutical intermediates and dye intermediates

ADA can also be used as a pharmaceutical intermediate and dye intermediate.

4, Hubei Xindesheng - a trusted supplier of ADA biological buffering agents

Hubei Xindesheng Material Technology Co., Ltd. has been deeply involved in the field of in vitro diagnostic raw materials for nearly 20 years. In the field of biological buffering agents, Xindesheng has a complete product line, covering dozens of commonly used and customized types such as Tris, HEPES, MOPS, Bicine, Caps, etc., with a daily output of up to 5 tons. The biological buffer products produced by the company have high purity, low impurities, and stable processes, which can accurately maintain the pH stability of the reaction system.

Xindesheng has established a comprehensive quality control system, strictly following standard operating procedures at every stage from raw material inspection to finished product inspection. The company is equipped with advanced production equipment and professional R&D laboratories, and the R&D team is composed of a group of high-quality professionals. In addition, Xindesheng can also provide personalized customized services according to customer needs.

5,Storage and usage recommendations

ADA biological buffer should be stored under sealed conditions at room temperature, away from light, and dry. When used, buffer solutions of corresponding concentrations can be prepared according to experimental requirements. High purity ADA products can effectively reduce inter batch differences, ensuring the stability and reproducibility of experimental results.

Hubei Xindesheng Material Technology Co., Ltd. is committed to providing high-quality biological buffer products for the global life science research and in vitro diagnostic industry. For more product information or technical support, please feel free to contact us through our official website.