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|Product Name:||ACETYL COENZYME A OXIDASE (ACOD)||Cas No.:||61116-22-1|
|Appearance:||Yellowish Amorphous Powder||Specific Activity:||≥5U/mg|
|Molecular Weigh:||Around 70KD||Isoelectronic Point:||6.2|
enzyme reactant complex,
citric acid crystals
ACOD Acetyl Coenzyme Oxidase Enzyme Substrate Product CAS No 61116-22-1
2. Enzyme Activity Definition
One unit of enzyme activity is equal to the amount of enzyme required to produce 1 micromole of hydrogen peroxide (corresponding to 1/2 micromole of quinone dye) in one minute at 37 degrees.
|Product Name:||ACETYL COENZYME A OXIDASE (ACOD)|
|Appearance:||Yellowish amorphous powder|
|Stability:||Store it below -20℃, it could be stable for one year|
|Molecular weight:||Around 70KD|
|Inhibitor:||Not prohibited by Sodium Azide|
|pH Stability:||6.0～8.5(37℃,20hr.) (Fig.3-1)|
|Most appropriate pH:||7.0～9.0 (Fig.3-2)|
|Most appropriate Temperature:||35℃～50℃ (Fig.3-3)|
|Heat stability:||Be stable under 45℃(15min.) (Fig.3-4)|
Application:Biochemical research. Free fatty acids (NEFA) are low in serum, and most of them bind to albumin. NEFA is directly related to oxidative stress, which can lead to diabetes by damaging islet cells and reducing the sensitivity of peripheral tissues to insulin. Concentrations of free fatty acids are elevated by diseases such as diabetes, severe liver disorders, hyperthyroidism, such as insulin resistance/type 2 diabetes, obesity, metabolic syndrome, and cardiovascular diseases.
Enzymatic detection of free fatty acids has the advantages of fast, simple, high sensitivity, easy automation and large-scale detection. Acetyl coenzyme A, the product of the first step reaction of NEFA enzyme detection kit by ACO oxidation, produces hydrogen peroxide, which provides a substrate for the next color reaction. Therefore, it has a very important role and significance.
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