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|Product Name:||ACETYL COENZYME A OXIDASE (ACOD)||Batch No.:||E008|
|Appearance:||White Amorphous Powder||Specific Activity:||≥1.0U/mg|
|PH Stability:||6.5～7.5||Molecular Weight:||60KD|
|Isoelectronic Point:||5.0||Most Appropriate Temperature:||37℃|
enzyme reactant complex,
citric acid crystals
Acs Acetyl Coenzyme A Synthetase Enzyme Substrate Product Specific Activity ≥1.0U / Mg
2. Enzyme Activity Definition
One unit of enzyme activity is equal to the amount of enzyme required to convert 1 micromolar palmitic acid in one minute at 37 degrees.
3. Accessory Figure 3.1,3.2,3.3,3.4
Application:Biochemical studies: Free fatty acids (NEFA) are low in serum, and most of them bind to albumin. NEFA is directly related to oxidative stress, which can lead to diabetes by damaging islet cells and reducing the sensitivity of peripheral tissues to insulin. Concentrations of free fatty acids are elevated by diseases such as diabetes, severe liver disorders, hyperthyroidism, such as insulin resistance/type 2 diabetes, obesity, metabolic syndrome, and cardiovascular diseases.
Enzymatic detection of free fatty acids has the advantages of fast, simple, high sensitivity, easy automation and mass detection. ACS is the first enzyme to start the free fatty acid enzymatic detection kit, so it has a very important role and significance.
4. Product specifications
|Product Name:||ACETYL COENZYME A OXIDASE (ACOD)|
|Appearance:||White amorphous powder|
|Stability:||Store it below -20℃, it could be stable for one year|
|Michaelis constant||Palmitic acid:1.1×10-5M；ATP:1.7×10-4M；CoA:3.2×10-4M|
|Inhibitor:||Not prohibited by Sodium Azide|
|pH Stability:||6.5～7.5 (Fig.3-1)|
|Most appropriate pH:||8.0 (Fig.3-2)|
|Most appropriate Temperature:||37℃ (Fig.3-3)|
|Heat stability:||Be stable under 50℃(2mM ATP) (Fig.3-4)|
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