As a new Trinder's chromogenic reagent, MAOS plays an important role in it. It is a water-soluble white powder. Its Chinese name is N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt monohydrate, which is sensitive to light and humidity. CAS No. 82692-97-5, purity requirement >99%, and molecular structure is shown in the following figure:
||N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline, sodium salt, monohydrate
||Room Temperature (25℃)
Room Temprature (25℃), avoiding direct sunlight and moisture
Application of MAOS
The new Trinder's reagent is a highly water-soluble aniline derivative, which is widely used in diagnostic detection and biochemical tests.There are several advantages over conventional chromogenic reagents in the colorimetric determination of hydrogen peroxide activity.The new Trinder's reagent is stable enough to be used both in solution and in test pipeline detection systems.In the presence of hydrogen peroxide and peroxidase, new Trinder's reagents form very stable purple or blue dyes during the oxidative coupling reaction with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazole sulfone hydrazone (MBTH).The molar absorbance of dyes coupled with MBTH is 1.5-2 times higher than that of dyes coupled with 4-AA; however, the 4-AA solution is more stable than that of MBTH solution.Substrates are enzymatically oxidized by their oxidase to produce hydrogen peroxide.This concentration of hydrogen peroxide corresponds to the substrate concentration.Therefore, the amount of the substrate can be determined by the color development of the oxidative coupling reaction.Glucose, alcohol, acyl-coenzyme A and cholesterol can be used to detect those substrates coupled with the new Trinder's reagent and 4-AA.There are 10 new Trinder's reagents available, and the use of MAOS members is also very important. For specific substrates, testing different kinds of new Trinder's reagents is necessary to develop the best detection system.
Oxidative chromogen reagents, highly water soluble, stable aniline analogues, chromogenic processes and oxidation reactions with a wide range of pH
Preparation of detection solution - MAOS (other substrate configuration methods are basically the same)
1. Dissolve 20 mg MAOS with 10 ml PBS to prepare 6.6 mM MAOS solution.(Specific solubility adjusted as required)
2. Dissolve 14 mg of 4-aminoantipyrine (4-AA) with 10 ml PBS to prepare 6.6 mM 4-AA solution.
3. Prepare 2 U/ml horseradish peroxidase solution with PBS.
4. Mix equal volumes of each solution to prepare the detection solution.Store the test solution in the dark at 4 C
1. Prepare sample solution for enzymatic oxidation reaction.The pH range of the buffer should be 5.5-9.5.
2. Prepare a standard solution containing a known amount of substrate using the same buffer.
3. Add the appropriate unit of oxidase to the sample solution, followed by an equal volume of detection solution.
4. Incubate the mixture at room temperature or 37 C for 30 minutes to 1 hour.
5. Determine the O.D. value at 555 nm.
6. Prepare a standard curve and determine the substrate concentration in the sample solution
MAOS belongs to one of the key sales varieties of Desheng Biochemical Co., Ltd. At the same time, new Trinder's reagents such as TOOS, TOPS, ADOS, ADPS, ALPS, DAOS, HDAOS, MADB and so on are available for everyone to choose. Desheng always adheres to the following principles: taking virtue as the basis, taking sincerity as the first; quality as the top, leading technology; customer as the respect, win-win symbiosis; pioneering and innovating, pursuing excellent business philosophy, and sincerely providing customers with excellent results.Quality products and services, welcome to consult and cooperate!