Heparin sodium, as a commonly used anticoagulant for blood collection, plays an important role in medical and laboratory research. However, some key issues need to be noted when using heparin sodium to ensure its effectiveness and safety. Let's take a look at the issues to pay attention to when using heparin sodium.
1. Selection of dosage: The dosage of heparin sodium should be determined based on the specific situation and purpose. Different biochemical projects may require different levels of heparin sodium. For example, the recommended dose range for conventional test tubes is 12-30IU of heparin sodium per milliliter of blood, and the anticoagulation of micro blood collectors can be less than 14IU per milliliter of blood. Therefore, suitable heparin sodium test tubes should be selected for blood collection based on the examination items before use.
2. Preparation and storage: Heparin sodium is generally supplied in the form of raw powder, and appropriate preparation and dissolution are required before use. Generally, deionized water can be used for dissolution. It is important to follow the recommendations in the product manual during the preparation process to ensure that the concentration of dissolved heparin sodium meets experimental or clinical requirements. The prepared heparin sodium solution should be added in a timely manner. If it needs to be placed, it should be stored in a sterile environment at 0-4 ℃ as much as possible. The addition should be completed within a week, avoiding direct sunlight to maintain its stability.
3. Addition method: Generally, the addition method is divided into machine and manual methods. Regardless of which method is used, attention should be paid to completing it in a sterile state. If using a machine, it is necessary to clean its conveying pipeline and container, and disinfect and air dry it with alcohol to ensure that there are no impurities, bacteria, or scale attached to the pipe wall.
4. Inverted mixing: To ensure sufficient anticoagulation effect on blood, the test tube should be inverted and mixed as soon as possible after blood collection, especially when the temperature of the blood collection environment is too high. If not inverted in a timely manner, it may cause abnormal test results.
5. Timely testing: Pay attention to completing the test within 6 hours, as heparin is a non irreversible anticoagulant. If the test is not completed within the specified time, it is prone to result errors.
In summary, as an important anticoagulant for blood collection, heparin sodium needs to be used with caution and follow professional advice. Reasonable dosage and correct usage methods will help ensure the effectiveness and safety of heparin sodium, thus providing reliable support for clinical and laboratory research.
As a supplier of heparin sodium raw materials, Desheng not only provides high-quality products, but also can customize production according to customers' specific needs to meet the requirements of different experiments, while paying attention to providing comprehensive after-sales service. If you have any interest, please feel free to contact us for purchase!
