Accurately locate the single product of biological buffer you need
Biological buffers have a very wide range of applications in the field of biochemical engineering, but we don't know much about it. Now we Desheng take you to understand the biological buffers we produce: Tris, BICINE, HEPES, CAPS, MOPS, etc. are used where and where are their advantages, so that you can clearly position when buying products. To the product you need, and help you get a general understanding of the product.
NO.1 tris (Tris(hydroxymethylaminomethane) or tromethamine)
Used in the preparation of buffers in biochemistry and molecular biology experiments; pharmaceutical intermediates; used in the preparation of surfactants and vulcanization accelerators; preparation of water-soluble polymers containing Tris structural units as coating dispersants; indoor formaldehyde adsorption Preparation of materials, etc.
NO.2 Bicine (N,N-dihydroxyethyl glycine)
Bicine is a very important buffer suitable for low-temperature biochemical environments. It can be used to prepare stable substrate solutions. It is one of the few buffers that can be used in special environments.
NO.3 HEPES (4-hydroxyethylpiperazine ethanesulfonic acid)
A non-toxic and harmless biological buffer, which can maintain a constant pH for a long time, and is loved by cell culturers.
NO.4 CAPS (3-(cyclohexylamine)-1-propanesulfonic acid)
In the application of nucleic acid hybridization, CAPS can reduce the yield of non-specific hybridization products and increase the specificity of nucleic acid hybridization; at the same time, it can maintain the yield of target hybridization products; it is useful in the identification of specific pathogens, the diagnosis of genetic diseases, and the analysis of gene sequences. Important value. It is commonly used as a buffer in detection kits, such as biochemical diagnostic kits and DNA/RNA extraction kits. It can also be used in a variety of environmentally friendly high-quality water-based two-component polyurethane coatings, and can be used in water-based isohydrogen ester curing agents. It can be combined with active groups (hydroxyl, carboxyl, amino, epoxy, etc.) Resin coexists stably for a long time.
NO.5 MOPS (3-morpholinopropane sulfonic acid)
MOPS does not form complexes with most metal ions, and is suitable for buffers in solution systems containing metal ions. It can be commonly used in the culture medium of bacteria, yeast and mammalian cells. It is also used as a running buffer in electrophoresis and a buffer in protein purification chromatography.
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