Adding and Using EDTA Dipotassium Salt
Blood testing is a routine examination in clinical medicine, and the vast majority of outpatient or physical examination personnel undergo this examination one by one. In order to ensure the accuracy of the examination results, manual testing has gradually developed from the beginning to fully automated testing. Ethylenediamine dipotassium salt, as a blood processing reagent, has significant anticoagulant effects and minimal impact on blood cell morphology. Therefore, it has become one of the highly respected and popular reagents in blood analysis. Next, we will introduce it in detail.
1、 Color of Ethylenediamine Dipotassium Salt Test Tube
The color of the test tube containing ethylenediamine dipotassium salt is purple. As an additive to prevent blood coagulation, it is generally called EDTA salt or ethylenediamine tetraacetic acid salt. Although ethylenediamine dipotassium salt can be added to purple test tubes, it should be noted that sometimes it may not be added to the purple tube, but it may also be ethylenediamine disodium salt. These two types of EDTA salts belong to the same category, and the purple tube can contain any of them. When used, it is usually distinguished based on the test tube label and testing items.
EDTA K2 Powder
2、 The effect of ethylenediamine dipotassium salt on blood
The effect on blood in test tubes is mainly reflected in its ability to fully protect blood cell components without affecting white blood cell count and size, and has a small impact on red blood cell morphology. At the same time, it can inhibit platelet aggregation, making it very suitable for general blood routine testing. However, it should be noted that ethylenediamine dipotassium salt test tubes cannot be used for coagulation experiments or platelet function tests, and should be inverted and mixed as soon as possible after blood collection to ensure sufficient anticoagulation.
3、 Addition and Use of Ethylenediamine Dipotassium Salt
The form of ethylenediamine dipotassium salt added to the blood collection vessel is a prepared aqueous solution. Generally, 4 milligrams of ethylenediamine dipotassium salt are required to anticoagulate 2 milliliters of blood. However, due to the low concentration, in order not to dilute the blood, a 200g/L aqueous solution containing ethylenediamine dipotassium salt is usually pre placed in each blood collection vessel μ。 At the same time, it is important to pay attention to the expiration date and usage environment during use. If there are changes in the usage environment, such as high or low temperatures, water can easily evaporate on the tube wall, especially plastic test tubes that may leak out of the tube wall and cause crystallization inside the tube. Therefore, it is necessary to mix them upside down during blood collection.
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