Correct blood collection and timely treatment are the key pre analysis steps to ensure the integrity of blood testing laboratory results. EDTA, sodium citrate and potassium oxalate are important components of blood anticoagulants. Combined with the feedback from the end customers of EDTA, sodium citrate and potassium oxalate, we discuss again how the blood collection materials of EDTA, sodium citrate and potassium oxalate in the additive of blood collection tube (BCT) can change the chemical test results.

1) EDTA salt ethylenediamine tetraacetate

EDTA can maintain glucose concentration by inhibiting glyceraldehyde-3-phosphate dehydrogenase. Considering that any kind of additive may change the stability of the analyte. As an anticoagulant and chelating agent, potassium EDTA can interfere with the determination of calcium and the production of blood clot, but it is preferred in hematological tests. As the upstream and downstream enterprises of blood vessel additives, manufacturers, sellers, clinical laboratory physicians and a series of practitioners must consider the pre analysis challenges in laboratory testing.

EDTA and metal ions are immunoassay reagents, while zinc and magnesium are enzyme cofactors of immunoassay reagents, which bind to alkaline phosphatase. Insufficient sample size will lead to a relative increase in EDTA content, which will increase the chelation between magnesium and zinc, thus affecting the reagent enzymes used for signal generation, such as alkaline phosphatase. The reagent antibody recognized the binding site of divalent cation complex on protein; Therefore, the decrease of calcium and magnesium levels may lead to conformational changes, thus reducing antibody binding.

2) Citrate, disodium citrate, trisodium citrate, potassium citrate

Sodium citrate condensation test preferably uses trisodium citrate in 3.2% (109 mmol / L) or 3.8% (129 mmol / L) solution. It can inhibit aspartate aminotransferase and alkaline phosphatase by cation chelation. Sodium citrate can inhibit platelet activation and measure the plasma level of platelet-derived components. Citrate buffer solution (pH < 5.9) can inhibit glycolysis immediately and keep glucose concentration.

3) Potassium oxalate

Potassium oxalate, another calcium chelating anticoagulant, often used in combination with anti glycolytic agents (sodium fluoride and sodium iodoacetate), can actually reduce hematocrit by up to 10% by inhaling water from cells into plasma. Oxalate can also inhibit several enzymes, such as amylase, lactate dehydrogenase, acid and alkaline phosphatase.

4) Summary

Desheng biochemical is a professional manufacturer of EDTA, sodium citrate and potassium oxalate. It has been deeply engaged in the industry for 15 years and is supported by a strong R & D team. Desheng biochemical suggested that employees should decide which anticoagulant to use according to the actual demand, so as to achieve the best detection effect.