Acridine ester derivatives are a class of chemiluminescence reagents with high quantum yield. Because of their low initiator requirements, low background and noise, and high sensitivity during the luminescence process, they are often used in clinical immunoassays. It can be used as nucleic acid molecular probes to measure the activity of biological enzymes or other applications. It can label antibodies and is widely used in immunoassays. Such compounds can quickly produce chemiluminescence in the presence of hydrogen peroxide and have high chemiluminescence efficiency. In addition, because acridine esters have a structure similar to acetophenone esters and have a single ester bond, the detection sensitivity is high, so they can be used for the detection of enzyme activity.
As an efficient chemiluminescent reagent, acridinium ester has a wide range of applications in clinical immunoassay, DNA analysis, biological enzyme activity determination, etc. This article focuses on the application of acridine esters in DNA determination. Acridinium esters can be used for genetic testing or microbial testing in terms of DNA.
Application of acridinium ester in DNA determination:
Acridine esters can be used to label oligonucleotide fragment probes for genetic or microbial assays. Acridine ester compounds are very suitable for labeling DNA strands to make chemiluminescent DNA probes. Modern medical research results show that many diseases such as cancer and genetic diseases are related to DNA mutations, and many infectious diseases are caused by viruses, bacteria or parasites in the environment. The analysis of specific sequence DNA of viruses is beneficial Control of the epidemic. The detection of DNA sequence and base mutations in its chain has far-reaching significance in gene screening, early diagnosis and treatment of genetic diseases, and pathogenic gene determination.
In nucleic acid hybridization analysis, the preparation of labeled probes with strong specificity and high sensitivity is the key to successful nucleic acid hybridization analysis. Acridinium ester derivatives can be directly labeled on nucleic acid probes without the need for catalysts and the luminescence quantum yield is not affected. In addition, under certain conditions, the labeled acridinium ester on the unhybridized single-stranded DNA is hydrolyzed and destroyed, and only the double-stranded protected acridinium ester formed by hybridization can produce chemiluminescence, and the entire hybridization process can be monitored without separation.
Based on this principle, some researchers have established a new gene microarray method for hybridization protection detection of aldehyde dehydrogenase and Alzheimer's disease-related gene ApoE. This method designed two biotin-labeled DNA probes to detect the A/G site polymorphism of aldehyde dehydrogenase, and three biotin-labeled DNA probes were used to detect C/T at two sites of ApoE Polymorphism, label the acridinium ester at the mutation site, and then fix the DNA probe on the microtiter plate coated with streptavidin. Only when the target DNA and the probe DNA are completely matched, can acridine be guaranteed The pyridine ester is not hydrolyzed, and the aldehyde dehydrogenase and ApoE genes can be determined based on the presence or absence of chemiluminescence. Wang Xiaojuan and others used acridine ester as a chemiluminescence indicator embedded in the double helix structure of DNA, and used 0.1mol/LHNO3 and 3%H2O2 as well as 0.3mol/LNaOH plus 2%TritonX-100 as the luminescence starting reagent, and established a simple evaluation A new method for chemiluminescence analysis of DNA break damage.
The results showed that low concentration of formaldehyde caused DNA breakage damage, high concentration of formaldehyde caused DNA strand cross-linking, and acetaldehyde only caused DNA strand breakage damage. At the same time, the damage behavior and possible mechanisms of formaldehyde and acetaldehyde to DNA were studied. Acridine esters are also used as DNA probes for the determination of HBV and HIV type I and II DNA, HIV-I DNA oligonucleotide probes for gag gene determination and immunoassays, DNA oligonucleotide probes for determination of ΔF508, ΔI507 Cystic fibrosis gene mutation, etc.
Desheng has been focusing on the development and production of chemiluminescent substrates for more than ten years. It can provide 6 kinds of acridinium ester products (acridinium ester DMAE-NHS, acridinium ester NSP-DMAE-NHS, acridinium salt NSP-SA , Acridine salt NSP-SA-NHS, acridine hydrazide NSP-SA-ADH, acridine ester ME-DMAE-NHS), as well as luminol and isoluminol. If you need it, you can click on the official website to consult our customer service or call us directly.
