Application of ADPS as a chromogenic substrate in biochemical reactions

In the field of biochemistry, chromogenic substrates are widely used as important chemical reagents for monitoring and analyzing various biochemical reactions. Among them, ADPS reagent(N-ethyl-N - (3-sulfopropyl) -3-methoxyaniline sodium salt dihydrate) has become an indispensable and important reagent in biochemical experiments due to its high sensitivity, ease of use, high reliability, and wide range of applications. This article will delve into the specific applications of ADPS in biochemical reactions, in order to provide useful references for its research and application in related fields.

Basic characteristics and principles of ADPS

ADPS is a white crystalline powder with high purity and high water solubility. The color development principle is based on enzyme catalyzed reactions, specifically, hydrogen peroxide (H ₂ O ₂) participates in the coupled oxidation reaction of substrate ADPS under the catalytic action of horseradish peroxidase (HRP) or peroxidase (POD). This reaction leads to the formation of quinone imine compounds in ADPS, resulting in a significant increase in the absorbance of the reaction system at specific wavelengths (such as 500nm). The degree of color change is directly proportional to the content of specific substances in the reaction system, thus achieving quantitative detection of the target substance.

The specific application of ADPS in biochemical reactions

1. Enzyme activity assay

ADPS plays an important role in enzyme activity assays. Enzyme activity is a measure of the ability of enzymes in an organism to catalyze chemical reactions, and is of great significance for understanding the metabolic processes and physiological functions of organisms. By measuring the degree of color change of ADPS in the reaction system, the activity level of the enzyme can be accurately reflected. For example, in the activity measurement of glucose oxidase, glucose is oxidized to gluconic acid and hydrogen peroxide under the catalysis of glucose oxidase, and the latter undergoes coupled oxidation reaction with ADPS under the catalysis of HRP, producing colored products. By measuring the absorbance of the product, the activity of glucose oxidase can be calculated.

2. Protein structure and function analysis

ADPS can also be used for the analysis of protein structure and function. Proteins are one of the most important biomolecules in living organisms, with a variety of biological functions. ADPS can be used to detect the interactions between proteins and enzymes or other molecules, thereby revealing the structural characteristics and functional mechanisms of proteins. For example, in the study of the interaction between proteins and enzymes, the affinity between proteins and enzymes can be evaluated by measuring the color change after the reaction between ADPS and enzymes. In addition, ADPS can also be used for monitoring protein conformational changes, providing important information for understanding protein function and regulatory mechanisms.

3. Biochemical reagent kit and diagnostic testing

ADPS also has a wide range of applications in biochemical test kits and diagnostic testing. Due to its high sensitivity and stability, ADPS is often used in various biochemical test kits, such as blood glucose test kits, creatinine test kits, etc. In these reagent kits, ADPS serves as a chromogenic substrate to accurately determine the content of the target substance by measuring the degree of color change. For example, in blood glucose detection, blood glucose is oxidized to gluconic acid and hydrogen peroxide under the catalysis of glucose oxidase, and the latter undergoes coupled oxidation reaction with ADPS under the catalysis of HRP, producing colored products. By measuring the absorbance of the product, the concentration of blood glucose can be calculated.

Advantages of ADPS application

1. High sensitivity: ADPS can quickly detect small changes in acidity or alkalinity or changes in the content of specific substances in the reaction system, making experimental results more accurate and reliable.

2. Easy to use: ADPS has good water solubility and can be easily formulated into solutions for use. At the same time, its color change is stable and easy to observe, making it convenient for experimenters to quickly judge the experimental results.

3. High reliability: There is a stable and reliable relationship between the color change of ADPS and the changes in acidity or specific substance content in the reaction system, which enables it to provide consistent and reproducible experimental results.

4. Wide range of applications: ADPS can be applied in various reaction systems, including various biochemical reactions. This enables it to be applied in multiple fields, such as medical research.

Conclusion

In summary, the chromogenic substrate ADPS has broad application prospects and important research value in biochemical reactions. Its high sensitivity, ease of use, high reliability, and wide range of applications make it an indispensable and important reagent in biochemical experiments. With the continuous development of scientific research technology, it is believed that ADPS will be applied and promoted in more fields. Meanwhile, the improvement and optimization of ADPS will also become one of the important directions for future research to better meet the needs of biochemical research and applications.

Desheng is a manufacturer of the new Trinder's reagents, currently producing a wide range of product types, including ADPS, which can be used in various fields and can also be customized according to the company's own usage. At the same time, the company is equipped with its own R&D team, which can provide a series of technical services for subsequent products. If you have any relevant needs, please click on the website or call to inquire about details!