The application of heparin lithium in blood testing is an important aspect of clinical diagnosis, playing a crucial role in blood collection and processing. Due to its extraction from pig intestinal mucosa, it is a mucopolysaccharide containing sulfate groups and a biologically active substance with strong negative charges, which has anticoagulant effects both in vivo and in vitro. Below, we will delve into the application of heparin lithium in blood.
Heparin lithium coagulation mechanism
Heparin lithium has an impact on all three stages of the coagulation process. Firstly, it binds to antithrombin III (AT - III) and forms a heparin AT - III complex, inactivating coagulation factors with serine protease activity, thereby inhibiting the formation of prothrombin kinase and preventing blood coagulation. In addition, it binds to the active center of thrombin at the reaction site of AT - III to form a stable thrombin antithrombin complex, which inactivates thrombin, inhibits the conversion of fibrinogen to fibrin, and also prevents platelet adhesion and aggregation.
Application fields of heparin lithium
1. Plasma separation tube: Heparin lithium is added to the PST plasma separation tube, which can achieve rapid separation of plasma. This is an ideal choice for electrolyte testing and is also suitable for routine plasma biochemical testing and emergency plasma biochemical testing.
2. Liver function examination: Heparin lithium is used in liver function examinations, such as detecting hepatitis, liver cancer, fatty liver, etc. The use of heparin lithium anticoagulation can effectively prevent blood clotting and improve examination efficiency.
3. Hemorheological testing: When detecting cardiovascular and cerebrovascular diseases, blood may exhibit abnormal viscosity, and heparin lithium can be used for anticoagulation. In addition, it is also used in some special projects, such as arterial blood gas analysis, electrolyte and calcium ion detection processes.
4. Blood gas and erythrocyte sedimentation rate detection: Heparin lithium is present in anticoagulant tubes, interfering with the coagulation process and prolonging the coagulation time of the sample. Therefore, it is also suitable for items such as blood gas analysis and erythrocyte sedimentation rate detection.
Precautions for use
1. The heparin lithium in the blood collection vessel needs to be thoroughly mixed with the blood sample to avoid partial coagulation.
2. The anticoagulant effect of heparin lithium is not irreversible, so the samples after blood collection should undergo relevant testing items as soon as possible, otherwise it will affect the test results.
3. Pay attention to the blood collection volume of heparin lithium tubes. Generally, 15-20IU of heparin lithium can anticoagulate 1ml of blood.
Heparin lithium plays an important role in clinical blood processing, and its anticoagulant properties make it an indispensable reagent in the medical field. As a professional manufacturer of heparin lithium, Desheng can provide analytical grade heparin lithium raw materials for preparation and use by blood collection manufacturers. At the same time, it has technical guidance and one-on-one service to timely solve customer problems. If you have any interest, please feel free to contact us for purchase!
