Application of PEP in the kit (CAS 35556-70-8)

PEP, namely phosphoenolpyruvate, is a very important substance in all life activities. It participates in many biochemical processes such as cell respiration and photosynthesis. The reagent we produce refers to its salt, phosphoenolpyruvate tricyclohexamine salt reagent.

Physical and chemical properties of PEP salt

English name: Phosphoenolpyruvic acid tricyclohexylammonium salt

Molecular weight: 465.56

Molecular formula: C21H44N306P

Molecular structure

Application of serum carbon dioxide determination kit

In the chloroplast, PEP can absorb carbon dioxide and convert it to oxaloacetate under the catalytic action of PEP carboxylase (carboxykinase). This process is the core of photosynthesis. The efficiency of PEP carboxylase to fix CO₂ is very high, and the reaction is very sensitive. With this feature, the content of trace carbon dioxide in serum can be determined, and the activity of PEP carboxylase in plant samples or serum or plasma can also be measured.

                                                             CO₂ fixation process of PEP in photosynthesis

Determination principle

PEP and bicarbonate (the form of serum CO₂) are catalyzed by enzyme to produce oxaloacetate. Oxaloacetate and NADH are catalyzed by malate dehydrogenase MDH to produce malate malate. NADH (nicotinamide) is measured with a spectrophotometer The reduced state of adenine dinucleotide, reduced coenzyme I) The absorbance at 340nm is attenuated, and the amount of attenuation is proportional to the concentration of CO₂, thereby measuring the serum CO₂ content. Similarly, the enzyme activity can be measured according to the rate of change of absorbance when a certain amount of CO₂ is generated, that is, a reagent kit for measuring the activity of a sample PEP carboxylase.

PEP is used in cell protectants and antioxidants

In cellular respiration, PEP participates in the last step of glycolysis, which is converted to pyruvate after removing high-energy phosphate groups. In the process of organ tissue refrigeration, it can reduce the oxidative stress and ATP consumption of tissue cells, reduce organ damage, and prolong the preservation time of clinical organs.

The use of PEP salt is not limited to this. Due to its carbon dioxide absorption and cell glycolysis characteristics, many applications are still under development. Desheng Technology's mature reagent is PEP tricyclohexylamine salt, mainly used Kit for carbon dioxide determination and PEP carboxylase activity determination.