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Application of acridine ester and luminol in chemiluminescence labeling tracking

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Application of acridine ester and luminol in chemiluminescence labeling tracking

The chemiluminescence labeling method represented by acridine ester and luminol is the preferred scheme for the quantitative detection of ligand conjugates. These assay markets have a high demand for technology, which requires detection technology to be highly sensitive, less susceptible to interference and easy to use. The chemiluminescence process has sufficient sensitivity, so it is very suitable for these applications. Old fashioned assays require the separation of bound markers from unbound markers. Although the assay is easier to develop, it is somewhat complex to implement. On the contrary, the chemiluminescence labeling method represented by acridine ester adopts homogeneous determination, which affects the luminescence reaction in some way through the combination of ligand and its receptor, which is easy to observe and the dose required for the reaction is small.

latest company news about Application of acridine ester and luminol in chemiluminescence labeling tracking  0

Chemiluminescence is a series of radioisotope based analytical procedures. Its feasibility is proved to be applicable to various analytes, including high molecular weight and low molecular weight substances. The stability and analytical performance of reagents are of great significance to improve and maintain high-quality analytical performance. Most importantly, the development of ICMA technology based on labeled antibodies has the potential to increase the sensitivity of determination. This technique may prove valuable in testing and monitoring infectious agents and tumor markers.

We have done experiments in which the natural luminol reaction product is incorporated into globulin, and the luminescence of its derivatives is 100 times that of diazonium salt. This also confirms the potential of Lumino.

 

Chemiluminescence of luminol and acridine ester occurs under alkaline conditions, although in the case of luminol, there are catalyst requirements. The catalyst can be a simple transition metal cation, such as Mn + + or Ni, but it can also be complex macromolecules, such as horseradish peroxidase and cytochrome. The most popular catalyst is micro peroxidase because of this efficient reaction. Under relatively mild conditions. This requirement for catalysts makes the reaction vulnerable to the interference of substances in biological samples and powerful.

 

Desheng bio produces chemiluminescent reagents, such as luminol, isoluminol, acridine ester, etc. In addition, it also independently develops and produces dipotassium and Tripotassium EDTA, with high purity, content of more than 99.5%, good anticoagulant effect and no impurities. After detection by SGS inspection organization, the content of trace elements is one order of magnitude lower than the national and industrial standards (10 times), with good clarity and high solubility. 80 grams of dipotassium and Tripotassium can be quickly dissolved per 100ml of water at 25 ℃, It can maintain the original properties of blood to the greatest extent and has no significant impact on the blood clinical test results. It has been favored and affirmed by well-known enterprises at home and abroad, including Yangpu!

Pub Time : 2021-08-18 14:12:45 >> News list
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