Applications of Luminol
Luminol, also known as 3-Aminophthalohydrazide, a kind of white powder at room temperature. It is a strong acid after being configured into a solution, which has a certain stimulating effect on eyes, skin and respiratory tract. When the luminescent ammonia base solution is treated with an oxidizing agent, the released energy exists in the form of photons, and the wavelength acts as a visible blue light. Therefore, when luminol is treated with nitrogen dioxide, PAN, hydrogen peroxide, ozone and other atmospheric oxidants, it will emit blue light. Based on this principle, luminol is the most common chemiluminescent substance.
The following will introduce you the applications of Luminol:
1.Luminol can be used for Arabidopsis thaliana immune-related reactions in Luminol peroxidase-based analysis.
2. As a chemiluminescent substrate Luminol Can be used to detect opsonization and phagocytosis function.
3. As a biodetector. It can be used to detect polymorphonuclear leukocyte (PMNL) response in patients with myeloperoxidase deficiency (MPO).
4. Metal cation analysis, using simple and inexpensive instruments with luminol, can detect metal ions at the part-per-billion level.
5. For glucocorticoid detection and analysis.
6.In biology, luminol is used to detect the presence of copper, iron and cyanide in cells. Usually, a mixed aqueous solution of hydrogen peroxide and a hydroxide base is used as stimulated agent.
7.Testing blood in forensic medicine.
Iron in hemoglobin in the Blood catalyze the decomposition of hydrogen peroxide, turning it into water and monooxygen. Monooxygen reoxidizes luminol to make it glow. When detecting blood stains, luminol reacts with heme emitting blue-green fluorescence. This detection method is extremely sensitive. The investigator sprays a solution of luminol and an activator in the area to be investigated, and iron in the blood immediately catalyzes the luminol reaction, which emit a blue light. After the bloodstain is treated with luminol, the DNA of the genetic material it contains has not been destroyed, and can be extracted for identification.When using luminol to detect blood stains, the standard forensic solution containing alkaline luminol and sodium perborate may react with industrial substances which contains copper and living substances such as pesticides, glues, parsnips, radishes to emit light . That can interfere with the detection of hemoglobin in the blood. In this case, the light-emitting substances can be distinguished by the wavelength of the light.
Hubei New Desheng Material Technology Co., Ltd. is a professional manufacturer of chemiluminescence reagent Luminol, which can be stored in the dark at 2-8°C for up to 12 months. After 17 years of R&D and production, our products are shipped daily to top research centers and biotech companies in Europe, America and Asia.
Contact Person: Miss. Ankiwang